##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939877_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2068 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.13346228239845 26.0 10.0 31.0 2.0 33.0 2 20.910058027079305 27.0 10.0 31.0 2.0 33.0 3 21.065280464216634 27.0 10.0 31.0 2.0 33.0 4 23.513539651837526 32.0 10.0 35.0 2.0 37.0 5 23.363636363636363 32.0 10.0 35.0 2.0 37.0 6 23.166344294003867 30.0 8.0 35.0 2.0 37.0 7 23.18568665377176 32.0 2.0 35.0 2.0 37.0 8 22.84816247582205 30.0 2.0 35.0 2.0 37.0 9 23.2683752417795 30.0 2.0 37.0 2.0 39.0 10-11 23.06068665377176 30.5 2.0 37.0 2.0 39.0 12-13 22.881286266924565 30.0 2.0 37.0 2.0 39.0 14-15 22.63056092843327 29.5 2.0 37.5 2.0 40.0 16-17 22.285299806576404 28.0 2.0 37.0 2.0 40.0 18-19 21.97437137330754 27.0 2.0 37.0 2.0 40.0 20-21 21.889023210831724 27.0 2.0 37.5 2.0 40.0 22-23 21.443665377176018 27.0 2.0 37.0 2.0 40.0 24-25 21.190764023210832 27.0 2.0 37.0 2.0 40.0 26-27 21.107833655705996 27.0 2.0 37.0 2.0 40.0 28-29 20.906189555125724 26.5 2.0 37.0 2.0 40.0 30-31 20.543278529980658 25.5 2.0 37.0 2.0 40.0 32-33 20.163926499032883 24.0 2.0 36.5 2.0 40.0 34-35 19.77998065764023 23.5 2.0 36.0 2.0 40.0 36-37 19.46808510638298 22.0 2.0 36.5 2.0 39.0 38-39 19.35880077369439 21.5 2.0 36.5 2.0 39.5 40-41 18.942214700193425 18.5 2.0 36.0 2.0 39.5 42-43 18.685203094777563 17.5 2.0 36.0 2.0 39.0 44-45 18.29303675048356 15.0 2.0 35.0 2.0 39.0 46-47 17.95551257253385 11.0 2.0 35.5 2.0 39.0 48-49 17.661025145067697 9.0 2.0 35.0 2.0 39.0 50-51 17.254110251450676 8.5 2.0 34.5 2.0 38.5 52-53 17.245647969052225 7.5 2.0 34.5 2.0 39.0 54-55 17.124274661508704 3.5 2.0 35.0 2.0 39.0 56-57 16.81237911025145 2.0 2.0 34.0 2.0 39.0 58-59 16.438346228239844 2.0 2.0 34.0 2.0 39.0 60-61 16.08389748549323 2.0 2.0 34.0 2.0 38.0 62-63 15.916586073500966 2.0 2.0 33.5 2.0 38.0 64-65 15.70188588007737 2.0 2.0 33.5 2.0 38.0 66-67 15.078094777562862 2.0 2.0 32.5 2.0 37.5 68-69 14.559961315280464 2.0 2.0 31.0 2.0 36.5 70-71 14.183268858800773 2.0 2.0 31.0 2.0 36.0 72-73 13.81407156673114 2.0 2.0 31.0 2.0 36.0 74-75 13.502901353965184 2.0 2.0 30.0 2.0 35.0 76-77 13.190764023210832 2.0 2.0 30.0 2.0 35.0 78-79 12.57301740812379 2.0 2.0 29.0 2.0 34.0 80-81 12.12161508704062 2.0 2.0 29.0 2.0 34.0 82-83 11.767166344294004 2.0 2.0 28.5 2.0 34.0 84-85 11.342359767891683 2.0 2.0 27.0 2.0 34.0 86-87 10.724613152804643 2.0 2.0 25.5 2.0 33.5 88-89 10.296905222437136 2.0 2.0 25.0 2.0 33.0 90-91 9.632736943907156 2.0 2.0 20.0 2.0 32.5 92-93 8.870889748549324 2.0 2.0 10.5 2.0 32.0 94-95 8.397485493230175 2.0 2.0 4.5 2.0 31.5 96-97 8.068907156673113 2.0 2.0 2.0 2.0 31.0 98-99 7.7645067698259185 2.0 2.0 2.0 2.0 31.0 100-101 7.0103965183752415 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 8.344858156028373 1102 2 9.515070921985817 1102 3 6.7854609929078045 1102 4 -12.671099290780145 1102 5 -4.485815602836883 1102 6 -15.1072695035461 1102 7 -7.813829787234049 1102 8 10.019503546099287 1102 9 12.234042553191493 1102 10-11 -20.396719858156033 1102 12-13 -19.659574468085104 1102 14-15 -19.48936170212766 1102 16-17 -18.566932624113477 1102 18-19 -18.93617021276596 1102 20-21 -18.999113475177303 1102 22-23 -17.88519503546099 1102 24-25 -17.96941489361702 1102 26-27 -17.67287234042553 1102 28-29 -17.11613475177305 1102 30-31 -17.25531914893617 1102 32-33 -16.58599290780142 1102 34-35 -16.06205673758865 1102 36-37 -15.359929078014183 1102 38-39 -15.142287234042556 1102 40-41 -15.242021276595747 1102 42-43 -15.40868794326241 1102 44-45 -14.70478723404256 1102 46-47 -15.15381205673759 1102 48-49 -15.15336879432624 1102 50-51 -14.338209219858154 1102 52-53 -13.865691489361701 1102 54-55 -13.764627659574465 1102 56-57 -13.302304964539008 1102 58-59 -12.762411347517729 1102 60-61 -12.438386524822697 1102 62-63 -12.371453900709218 1102 64-65 -12.03900709219858 1102 66-67 -12.113918439716311 1102 68-69 -10.851507092198585 1102 70-71 -11.145833333333332 1102 72-73 -10.48359929078014 1102 74-75 -9.868794326241135 1102 76-77 -9.502216312056738 1102 78-79 -9.38519503546099 1102 80-81 -8.48049645390071 1102 82-83 -8.230053191489361 1102 84-85 -7.529255319148936 1102 86-87 -6.535460992907803 1102 88-89 -6.198581560283685 1102 90-91 -5.848847517730497 1102 92-93 -5.3528368794326235 1102 94-95 -4.862145390070921 1102 96-97 -4.617021276595745 1102 98-99 -4.519060283687941 1102 100-101 -3.6445035460992914 1103 1 -9.655141843971627 1103 2 -10.484929078014183 1103 3 -4.2145390070921955 1103 4 -12.671099290780145 1103 5 -4.485815602836883 1103 6 9.8927304964539 1103 7 6.186170212765951 1103 8 -3.980496453900713 1103 9 9.234042553191493 1103 10-11 -2.8967198581560325 1103 12-13 -19.659574468085104 1103 14-15 -19.48936170212766 1103 16-17 -18.566932624113477 1103 18-19 -18.93617021276596 1103 20-21 -18.999113475177303 1103 22-23 -17.88519503546099 1103 24-25 -17.96941489361702 1103 26-27 -17.67287234042553 1103 28-29 -17.11613475177305 1103 30-31 -17.25531914893617 1103 32-33 -16.58599290780142 1103 34-35 -16.06205673758865 1103 36-37 -15.359929078014183 1103 38-39 -15.142287234042556 1103 40-41 -15.242021276595747 1103 42-43 -15.40868794326241 1103 44-45 -14.70478723404256 1103 46-47 -15.15381205673759 1103 48-49 -15.15336879432624 1103 50-51 -14.338209219858154 1103 52-53 -13.865691489361701 1103 54-55 -13.764627659574465 1103 56-57 -13.302304964539008 1103 58-59 -12.762411347517729 1103 60-61 -12.438386524822697 1103 62-63 -12.371453900709218 1103 64-65 -12.03900709219858 1103 66-67 -12.113918439716311 1103 68-69 -10.851507092198585 1103 70-71 -11.145833333333332 1103 72-73 -10.48359929078014 1103 74-75 -9.868794326241135 1103 76-77 -9.502216312056738 1103 78-79 -9.38519503546099 1103 80-81 -8.48049645390071 1103 82-83 -8.230053191489361 1103 84-85 -7.529255319148936 1103 86-87 -6.535460992907803 1103 88-89 -6.198581560283685 1103 90-91 -5.848847517730497 1103 92-93 -5.3528368794326235 1103 94-95 -4.862145390070921 1103 96-97 -4.617021276595745 1103 98-99 -4.519060283687941 1103 100-101 -3.6445035460992914 1104 1 -13.655141843971627 1104 2 -5.484929078014183 1104 3 -4.2145390070921955 1104 4 -4.171099290780145 1104 5 -14.485815602836883 1104 6 -9.1072695035461 1104 7 -7.813829787234049 1104 8 -15.480496453900713 1104 9 -7.765957446808507 1104 10-11 -5.3967198581560325 1104 12-13 -3.659574468085104 1104 14-15 -4.23936170212766 1104 16-17 -8.316932624113477 1104 18-19 -11.186170212765958 1104 20-21 -10.749113475177303 1104 22-23 -13.885195035460988 1104 24-25 -3.719414893617021 1104 26-27 -1.4228723404255312 1104 28-29 1.1338652482269502 1104 30-31 -3.00531914893617 1104 32-33 -6.585992907801419 1104 34-35 -10.812056737588652 1104 36-37 -3.109929078014183 1104 38-39 -6.642287234042556 1104 40-41 -8.492021276595747 1104 42-43 -0.6586879432624109 1104 44-45 -6.454787234042559 1104 46-47 -15.15381205673759 1104 48-49 -15.15336879432624 1104 50-51 -14.338209219858154 1104 52-53 -13.865691489361701 1104 54-55 -13.764627659574465 1104 56-57 -13.302304964539008 1104 58-59 -12.762411347517729 1104 60-61 -12.438386524822697 1104 62-63 -12.371453900709218 1104 64-65 -12.03900709219858 1104 66-67 -12.113918439716311 1104 68-69 -10.851507092198585 1104 70-71 -11.145833333333332 1104 72-73 -10.48359929078014 1104 74-75 -9.868794326241135 1104 76-77 -9.502216312056738 1104 78-79 -9.38519503546099 1104 80-81 -8.48049645390071 1104 82-83 -8.230053191489361 1104 84-85 -7.529255319148936 1104 86-87 -6.535460992907803 1104 88-89 -6.198581560283685 1104 90-91 -5.848847517730497 1104 92-93 -5.3528368794326235 1104 94-95 -4.862145390070921 1104 96-97 -4.617021276595745 1104 98-99 -4.519060283687941 1104 100-101 -3.6445035460992914 1105 1 10.844858156028373 1105 2 9.515070921985817 1105 3 8.785460992907804 1105 4 12.328900709219855 1105 5 9.014184397163117 1105 6 10.3927304964539 1105 7 8.186170212765951 1105 8 6.519503546099287 1105 9 11.734042553191493 1105 10-11 8.103280141843967 1105 12-13 8.840425531914896 1105 14-15 13.26063829787234 1105 16-17 13.183067375886523 1105 18-19 13.563829787234042 1105 20-21 11.750886524822697 1105 22-23 17.11480496453901 1105 24-25 15.28058510638298 1105 26-27 3.8271276595744688 1105 28-29 6.38386524822695 1105 30-31 6.24468085106383 1105 32-33 5.914007092198581 1105 34-35 -2.3120567375886516 1105 36-37 13.890070921985817 1105 38-39 6.857712765957444 1105 40-41 13.257978723404253 1105 42-43 1.5913120567375891 1105 44-45 11.29521276595744 1105 46-47 18.84618794326241 1105 48-49 5.846631205673759 1105 50-51 6.411790780141846 1105 52-53 15.884308510638299 1105 54-55 20.735372340425535 1105 56-57 18.197695035460992 1105 58-59 10.487588652482271 1105 60-61 13.561613475177303 1105 62-63 12.628546099290782 1105 64-65 15.46099290780142 1105 66-67 17.63608156028369 1105 68-69 14.398492907801415 1105 70-71 15.354166666666668 1105 72-73 11.01640070921986 1105 74-75 3.381205673758865 1105 76-77 2.4977836879432616 1105 78-79 4.36480496453901 1105 80-81 6.0195035460992905 1105 82-83 3.019946808510639 1105 84-85 5.970744680851064 1105 86-87 7.214539007092197 1105 88-89 7.801418439716315 1105 90-91 9.151152482269502 1105 92-93 3.6471631205673765 1105 94-95 7.637854609929079 1105 96-97 10.882978723404255 1105 98-99 7.730939716312059 1105 100-101 9.60549645390071 1106 1 10.344858156028373 1106 2 7.515070921985817 1106 3 9.785460992907804 1106 4 10.328900709219855 1106 5 9.514184397163117 1106 6 11.8927304964539 1106 7 10.186170212765951 1106 8 8.019503546099287 1106 9 10.234042553191493 1106 10-11 7.6032801418439675 1106 12-13 0.3404255319148959 1106 14-15 14.51063829787234 1106 16-17 17.433067375886523 1106 18-19 18.56382978723404 1106 20-21 8.500886524822697 1106 22-23 14.114804964539012 1106 24-25 12.03058510638298 1106 26-27 17.82712765957447 1106 28-29 4.38386524822695 1106 30-31 8.24468085106383 1106 32-33 14.914007092198581 1106 34-35 16.93794326241135 1106 36-37 4.640070921985817 1106 38-39 -1.1422872340425556 1106 40-41 13.257978723404253 1106 42-43 17.09131205673759 1106 44-45 21.79521276595744 1106 46-47 13.34618794326241 1106 48-49 18.84663120567376 1106 50-51 17.161790780141846 1106 52-53 14.134308510638299 1106 54-55 14.235372340425535 1106 56-57 20.197695035460992 1106 58-59 19.237588652482273 1106 60-61 17.561613475177303 1106 62-63 22.12854609929078 1106 64-65 13.46099290780142 1106 66-67 20.38608156028369 1106 68-69 14.648492907801415 1106 70-71 19.354166666666668 1106 72-73 12.51640070921986 1106 74-75 16.131205673758863 1106 76-77 14.997783687943262 1106 78-79 26.11480496453901 1106 80-81 23.01950354609929 1106 82-83 13.769946808510639 1106 84-85 14.470744680851064 1106 86-87 21.4645390070922 1106 88-89 8.301418439716315 1106 90-91 -5.848847517730497 1106 92-93 -5.3528368794326235 1106 94-95 -4.862145390070921 1106 96-97 -4.617021276595745 1106 98-99 -4.519060283687941 1106 100-101 -3.6445035460992914 1107 1 11.344858156028373 1107 2 10.515070921985817 1107 3 13.785460992907804 1107 4 14.328900709219855 1107 5 13.514184397163117 1107 6 11.8927304964539 1107 7 14.186170212765951 1107 8 13.019503546099287 1107 9 14.234042553191493 1107 10-11 15.603280141843967 1107 12-13 16.840425531914896 1107 14-15 18.01063829787234 1107 16-17 19.433067375886523 1107 18-19 20.06382978723404 1107 20-21 18.000886524822697 1107 22-23 20.61480496453901 1107 24-25 17.03058510638298 1107 26-27 17.82712765957447 1107 28-29 17.38386524822695 1107 30-31 18.74468085106383 1107 32-33 16.91400709219858 1107 34-35 12.937943262411348 1107 36-37 15.140070921985817 1107 38-39 15.357712765957444 1107 40-41 17.757978723404253 1107 42-43 6.091312056737589 1107 44-45 10.29521276595744 1107 46-47 17.34618794326241 1107 48-49 14.34663120567376 1107 50-51 8.661790780141846 1107 52-53 10.634308510638299 1107 54-55 10.735372340425535 1107 56-57 10.197695035460992 1107 58-59 16.237588652482273 1107 60-61 20.561613475177303 1107 62-63 18.12854609929078 1107 64-65 13.96099290780142 1107 66-67 10.386081560283689 1107 68-69 15.148492907801415 1107 70-71 10.854166666666668 1107 72-73 6.516400709219861 1107 74-75 10.131205673758865 1107 76-77 9.497783687943262 1107 78-79 18.61480496453901 1107 80-81 20.51950354609929 1107 82-83 13.269946808510639 1107 84-85 21.970744680851062 1107 86-87 23.9645390070922 1107 88-89 21.301418439716315 1107 90-91 23.651152482269502 1107 92-93 22.647163120567377 1107 94-95 24.13785460992908 1107 96-97 25.882978723404257 1107 98-99 9.980939716312058 1107 100-101 -3.6445035460992914 1108 1 -3.6551418439716272 1108 2 0.5150709219858172 1108 3 -0.5478723404255277 1108 4 -0.004432624113476891 1108 5 -1.152482269503551 1108 6 -5.1072695035460995 1108 7 -5.813829787234049 1108 8 -6.313829787234047 1108 9 -6.765957446808507 1108 10-11 2.4366134751772996 1108 12-13 1.8404255319148959 1108 14-15 1.0106382978723403 1108 16-17 0.26640070921985526 1108 18-19 -3.102836879432626 1108 20-21 -5.165780141843969 1108 22-23 -12.21852836879432 1108 24-25 -7.969414893617021 1108 26-27 -12.006205673758863 1108 28-29 -8.949468085106384 1108 30-31 -6.25531914893617 1108 32-33 -16.58599290780142 1108 34-35 -16.06205673758865 1108 36-37 -15.359929078014183 1108 38-39 -15.142287234042556 1108 40-41 -15.242021276595747 1108 42-43 -15.40868794326241 1108 44-45 -14.70478723404256 1108 46-47 -15.15381205673759 1108 48-49 -15.15336879432624 1108 50-51 -14.338209219858154 1108 52-53 -13.865691489361701 1108 54-55 -13.764627659574465 1108 56-57 -13.302304964539008 1108 58-59 -12.762411347517729 1108 60-61 -12.438386524822697 1108 62-63 -12.371453900709218 1108 64-65 -12.03900709219858 1108 66-67 -12.113918439716311 1108 68-69 -10.851507092198585 1108 70-71 -11.145833333333332 1108 72-73 -10.48359929078014 1108 74-75 -9.868794326241135 1108 76-77 -9.502216312056738 1108 78-79 -9.38519503546099 1108 80-81 -8.48049645390071 1108 82-83 -8.230053191489361 1108 84-85 -7.529255319148936 1108 86-87 -6.535460992907803 1108 88-89 -6.198581560283685 1108 90-91 -5.848847517730497 1108 92-93 -5.3528368794326235 1108 94-95 -4.862145390070921 1108 96-97 -4.617021276595745 1108 98-99 -4.519060283687941 1108 100-101 -3.6445035460992914 1109 1 11.344858156028373 1109 2 10.515070921985817 1109 3 12.285460992907804 1109 4 14.328900709219855 1109 5 13.514184397163117 1109 6 12.8927304964539 1109 7 14.186170212765951 1109 8 14.519503546099287 1109 9 15.734042553191493 1109 10-11 14.353280141843967 1109 12-13 12.840425531914896 1109 14-15 8.26063829787234 1109 16-17 8.433067375886523 1109 18-19 13.563829787234042 1109 20-21 11.250886524822697 1109 22-23 10.864804964539012 1109 24-25 12.28058510638298 1109 26-27 8.827127659574469 1109 28-29 15.38386524822695 1109 30-31 16.24468085106383 1109 32-33 16.66400709219858 1109 34-35 18.18794326241135 1109 36-37 13.640070921985817 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96-97 -4.617021276595745 2314 98-99 -4.519060283687941 2314 100-101 -3.6445035460992914 2315 1 -9.655141843971627 2315 2 4.515070921985817 2315 3 -10.214539007092196 2315 4 -3.6710992907801447 2315 5 -4.485815602836883 2315 6 -6.1072695035460995 2315 7 -5.813829787234049 2315 8 -4.980496453900713 2315 9 9.234042553191493 2315 10-11 12.103280141843967 2315 12-13 -19.659574468085104 2315 14-15 -19.48936170212766 2315 16-17 -18.566932624113477 2315 18-19 -18.93617021276596 2315 20-21 -18.999113475177303 2315 22-23 -17.88519503546099 2315 24-25 -17.96941489361702 2315 26-27 -17.67287234042553 2315 28-29 -17.11613475177305 2315 30-31 -17.25531914893617 2315 32-33 -16.58599290780142 2315 34-35 -16.06205673758865 2315 36-37 -15.359929078014183 2315 38-39 -15.142287234042556 2315 40-41 -15.242021276595747 2315 42-43 -15.40868794326241 2315 44-45 -14.70478723404256 2315 46-47 -15.15381205673759 2315 48-49 -15.15336879432624 2315 50-51 -14.338209219858154 2315 52-53 -13.865691489361701 2315 54-55 -13.764627659574465 2315 56-57 -13.302304964539008 2315 58-59 -12.762411347517729 2315 60-61 -12.438386524822697 2315 62-63 -12.371453900709218 2315 64-65 -12.03900709219858 2315 66-67 -12.113918439716311 2315 68-69 -10.851507092198585 2315 70-71 -11.145833333333332 2315 72-73 -10.48359929078014 2315 74-75 -9.868794326241135 2315 76-77 -9.502216312056738 2315 78-79 -9.38519503546099 2315 80-81 -8.48049645390071 2315 82-83 -8.230053191489361 2315 84-85 -7.529255319148936 2315 86-87 -6.535460992907803 2315 88-89 -6.198581560283685 2315 90-91 -5.848847517730497 2315 92-93 -5.3528368794326235 2315 94-95 -4.862145390070921 2315 96-97 -4.617021276595745 2315 98-99 -4.519060283687941 2315 100-101 -3.6445035460992914 2316 1 0.3448581560283728 2316 2 -7.484929078014183 2316 3 -4.2145390070921955 2316 4 -8.171099290780145 2316 5 -4.485815602836883 2316 6 -2.1072695035460995 2316 7 -0.8138297872340488 2316 8 0.519503546099287 2316 9 9.234042553191493 2316 10-11 1.8532801418439675 2316 12-13 6.340425531914896 2316 14-15 -8.23936170212766 2316 16-17 -4.816932624113477 2316 18-19 -5.686170212765958 2316 20-21 -18.999113475177303 2316 22-23 -17.88519503546099 2316 24-25 -17.96941489361702 2316 26-27 -17.67287234042553 2316 28-29 -17.11613475177305 2316 30-31 -17.25531914893617 2316 32-33 -16.58599290780142 2316 34-35 -16.06205673758865 2316 36-37 -15.359929078014183 2316 38-39 -15.142287234042556 2316 40-41 -15.242021276595747 2316 42-43 -15.40868794326241 2316 44-45 -14.70478723404256 2316 46-47 -15.15381205673759 2316 48-49 -15.15336879432624 2316 50-51 -14.338209219858154 2316 52-53 -13.865691489361701 2316 54-55 -13.764627659574465 2316 56-57 -13.302304964539008 2316 58-59 -12.762411347517729 2316 60-61 -12.438386524822697 2316 62-63 -12.371453900709218 2316 64-65 -12.03900709219858 2316 66-67 -12.113918439716311 2316 68-69 -10.851507092198585 2316 70-71 -11.145833333333332 2316 72-73 -10.48359929078014 2316 74-75 -9.868794326241135 2316 76-77 -9.502216312056738 2316 78-79 -9.38519503546099 2316 80-81 -8.48049645390071 2316 82-83 -8.230053191489361 2316 84-85 -7.529255319148936 2316 86-87 -6.535460992907803 2316 88-89 -6.198581560283685 2316 90-91 -5.848847517730497 2316 92-93 -5.3528368794326235 2316 94-95 -4.862145390070921 2316 96-97 -4.617021276595745 2316 98-99 -4.519060283687941 2316 100-101 -3.6445035460992914 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 491.0 3 85.0 4 58.0 5 56.0 6 40.0 7 60.0 8 37.0 9 33.0 10 28.0 11 31.0 12 51.0 13 33.0 14 26.0 15 35.0 16 30.0 17 27.0 18 25.0 19 34.0 20 28.0 21 38.0 22 27.0 23 38.0 24 36.0 25 37.0 26 43.0 27 60.0 28 51.0 29 54.0 30 55.0 31 65.0 32 58.0 33 56.0 34 75.0 35 62.0 36 60.0 37 37.0 38 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.577314590402324 20.116335433834223 13.911778962675713 22.394571013087734 2 17.4503150751333 22.782355792535142 37.61512360639845 22.15220552593311 3 22.34609791565681 25.206010664081436 27.193407658749397 25.254483761512358 4 16.093068347067376 18.177411536597187 35.04604944255938 30.683470673776053 5 15.996122152205524 37.66359670382938 30.538051381483278 15.802229762481822 6 32.34723569350145 35.93598448108632 15.761396702230842 15.955383123181377 7 30.940834141610086 30.940834141610086 20.61105722599418 17.507274490785647 8 27.982541222114456 33.17167798254122 18.962172647914645 19.88360814742968 9 28.855480116391853 18.28322017458778 20.46556741028128 32.39573229873909 10-11 25.630455868089236 27.424830261881667 26.212415130940837 20.732298739088264 12-13 27.78855480116392 26.576139670223085 24.733268671193017 20.90203685741998 14-15 25.7274490785645 27.036857419980603 24.19980601357905 23.035887487875847 16-17 24.612027158098932 29.24345295829292 24.587778855480117 21.55674102812803 18-19 23.714839961202717 27.885548011639184 26.867119301648884 21.532492725509215 20-21 24.61799660441426 27.33446519524618 26.99490662139219 21.052631578947366 22-23 25.157690441533237 26.322173702086367 26.92867540029112 21.591460456089276 24-25 24.290220820189273 26.42562484833778 27.202135404028148 22.082018927444793 26-27 23.20388349514563 27.20873786407767 28.7378640776699 20.849514563106798 28-29 22.184466019417474 27.378640776699033 29.36893203883495 21.067961165048544 30-31 23.95631067961165 25.898058252427187 28.398058252427184 21.74757281553398 32-33 23.373786407766993 27.45145631067961 27.47572815533981 21.699029126213592 34-35 23.95631067961165 26.941747572815533 27.669902912621357 21.432038834951456 36-37 23.392380490172286 27.784518320795925 26.377092938607134 22.446008250424654 38-39 22.518806115020627 27.808784275661246 27.663188546469303 22.009221062848823 40-41 26.322173702086367 26.637554585152838 26.540514313440077 20.499757399320718 42-43 23.926231497209415 26.59548653239505 28.24557146323708 21.232710507158455 44-45 24.163027656477436 26.952935468219312 26.80737506065017 22.076661814653082 46-47 25.16990291262136 26.820388349514563 26.06796116504854 21.941747572815533 48-49 23.689320388349515 28.058252427184467 26.019417475728158 22.233009708737864 50-51 24.684466019417474 26.480582524271846 27.16019417475728 21.674757281553397 52-53 23.737864077669904 27.548543689320386 27.111650485436893 21.601941747572813 54-55 24.393203883495147 26.820388349514563 26.62621359223301 22.16019417475728 56-57 23.980582524271846 27.71844660194175 25.436893203883493 22.864077669902912 58-59 24.490291262135923 26.359223300970875 26.09223300970874 23.058252427184467 60-61 23.847646773410965 27.341096555070354 25.812712275594375 22.99854439592431 62-63 24.61799660441426 28.110599078341014 25.248605384428814 22.02279893281591 64-65 24.38136826783115 26.9771955361475 25.37603105288695 23.2654051431344 66-67 24.411550594515894 27.008007765105557 24.8240718272264 23.756369813152148 68-69 24.799805872361077 27.056539674836205 24.993933511283668 23.149720941519046 70-71 24.12035913613201 26.789614171317645 25.212327105071587 23.87769958747877 72-73 24.83009708737864 26.2621359223301 24.5873786407767 24.320388349514563 74-75 24.004854368932037 27.233009708737864 25.218446601941746 23.54368932038835 76-77 24.126213592233007 27.791262135922327 24.004854368932037 24.07766990291262 78-79 24.417475728155342 26.941747572815533 24.563106796116504 24.07766990291262 80-81 24.393203883495147 26.601941747572816 24.927184466019416 24.07766990291262 82-83 24.75728155339806 26.820388349514563 24.563106796116504 23.859223300970875 84-85 25.04854368932039 27.063106796116504 24.247572815533978 23.640776699029125 86-87 23.519417475728154 27.184466019417474 25.0 24.296116504854368 88-89 24.271844660194176 25.8252427184466 24.805825242718445 25.097087378640776 90-91 25.0546780072904 26.318347509113 25.6865127582017 22.940461725394897 92-93 25.57856272838002 25.77344701583435 24.3605359317905 24.287454323995128 94-95 24.91500728508985 26.59057795046139 23.409422049538612 25.084992714910147 96-97 24.07766990291262 27.014563106796118 24.004854368932037 24.902912621359224 98-99 24.368932038834952 26.480582524271846 24.75728155339806 24.393203883495147 100-101 25.776699029126217 26.06796116504854 24.150485436893206 24.004854368932037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 3.5 2 1.0 3 0.5 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 2.0 10 2.0 11 1.5 12 2.0 13 1.5 14 1.5 15 2.0 16 4.5 17 4.5 18 1.5 19 3.0 20 4.0 21 2.0 22 1.5 23 3.5 24 5.5 25 5.5 26 7.5 27 12.0 28 10.0 29 12.5 30 18.5 31 17.0 32 24.0 33 28.0 34 26.0 35 30.5 36 40.0 37 44.5 38 48.0 39 60.0 40 65.0 41 60.0 42 57.5 43 60.5 44 62.5 45 69.0 46 75.0 47 80.0 48 78.0 49 74.5 50 81.5 51 88.0 52 84.0 53 75.5 54 71.5 55 73.0 56 72.0 57 61.5 58 45.0 59 37.0 60 44.5 61 40.0 62 27.5 63 30.0 64 28.0 65 23.0 66 19.0 67 12.0 68 9.0 69 5.5 70 4.0 71 4.5 72 6.0 73 4.0 74 1.0 75 0.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24177949709864605 2 0.24177949709864605 3 0.24177949709864605 4 0.24177949709864605 5 0.24177949709864605 6 0.2901353965183753 7 0.2901353965183753 8 0.2901353965183753 9 0.2901353965183753 10-11 0.2901353965183753 12-13 0.2901353965183753 14-15 0.2901353965183753 16-17 0.2901353965183753 18-19 0.2901353965183753 20-21 0.3143133462282398 22-23 0.33849129593810445 24-25 0.36266924564796904 26-27 0.3868471953578337 28-29 0.3868471953578337 30-31 0.3868471953578337 32-33 0.3868471953578337 34-35 0.3868471953578337 36-37 0.36266924564796904 38-39 0.36266924564796904 40-41 0.33849129593810445 42-43 0.36266924564796904 44-45 0.33849129593810445 46-47 0.3868471953578337 48-49 0.3868471953578337 50-51 0.3868471953578337 52-53 0.3868471953578337 54-55 0.3868471953578337 56-57 0.3868471953578337 58-59 0.3868471953578337 60-61 0.33849129593810445 62-63 0.3143133462282398 64-65 0.33849129593810445 66-67 0.36266924564796904 68-69 0.36266924564796904 70-71 0.36266924564796904 72-73 0.3868471953578337 74-75 0.3868471953578337 76-77 0.3868471953578337 78-79 0.3868471953578337 80-81 0.3868471953578337 82-83 0.3868471953578337 84-85 0.3868471953578337 86-87 0.3868471953578337 88-89 0.3868471953578337 90-91 0.5077369439071566 92-93 0.7495164410058027 94-95 0.43520309477756286 96-97 0.3868471953578337 98-99 0.3868471953578337 100-101 0.3868471953578337 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2068.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.12959381044487 #Duplication Level Percentage of deduplicated Percentage of total 1 99.46341463414635 98.59767891682786 2 0.3902439024390244 0.7736943907156674 3 0.04878048780487805 0.14506769825918764 4 0.0 0.0 5 0.0975609756097561 0.4835589941972921 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.24177949709864605 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5 0.24177949709864605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.14506769825918764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.09671179883945841 0.0 34-35 0.0 0.0 0.0 0.21760154738878143 0.0 36-37 0.0 0.0 0.0 0.33849129593810445 0.0 38-39 0.0 0.0 0.0 0.43520309477756286 0.0 40-41 0.0 0.0 0.0 0.6044487427466151 0.0 42-43 0.0 0.0 0.0 0.7011605415860735 0.0 44-45 0.0 0.0 0.0 0.7736943907156673 0.0 46-47 0.0 0.0 0.0 0.8945841392649904 0.0 48-49 0.0 0.0 0.0 1.160541586073501 0.0 50-51 0.0 0.0 0.0 1.4264990328820115 0.0 52-53 0.0 0.0 0.0 1.5957446808510638 0.0 54-55 0.0 0.0 0.0 1.6682785299806575 0.0 56-57 0.0 0.0 0.0 1.8133462282398454 0.0 58-59 0.0 0.0 0.0 1.8617021276595744 0.0 60-61 0.0 0.0 0.0 2.006769825918762 0.0 62-63 0.0 0.0 0.0 2.2727272727272725 0.0 64-65 0.0 0.0 0.0 2.5386847195357833 0.0 66-67 0.0 0.0 0.0 2.804642166344294 0.0 68-69 0.0 0.0 0.0 2.973887814313346 0.0 70-71 0.0 0.0 0.0 3.1914893617021276 0.0 72-73 0.0 0.0 0.0 3.578336557059961 0.0 74-75 0.0 0.0 0.0 4.182785299806577 0.0 76-77 0.0 0.0 0.0 4.376208897485493 0.0 78-79 0.0 0.0 0.0 4.5212765957446805 0.0 80-81 0.0 0.0 0.0 5.029013539651837 0.0 82-83 0.0 0.0 0.0 5.319148936170213 0.0 84-85 0.0 0.0 0.0 5.633462282398453 0.0 86-87 0.0 0.0 0.0 5.947775628626692 0.0 88-89 0.0 0.0 0.0 6.262088974854932 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE