FastQCFastQC Report
Fri 27 May 2016
SRR939877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG120.5802707930367506RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG120.5802707930367506RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.4835589941972921No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT70.33849129593810445No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT60.2901353965183753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2901353965183753No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC50.24177949709864605No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG50.24177949709864605TruSeq Adapter, Index 3 (96% over 28bp)
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA50.24177949709864605No Hit
TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATA50.24177949709864605No Hit
ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA50.24177949709864605No Hit
GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA50.24177949709864605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.19342359767891684No Hit
TCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATAC40.19342359767891684No Hit
GTAGAGATGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCT40.19342359767891684No Hit
GTTCAAGCCGCCGCAGGGAGGTGTGGGTCTCCGGCCCCTCGTAGTTGTGT40.19342359767891684No Hit
GATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCACACTGA40.19342359767891684No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA30.14506769825918764TruSeq Adapter, Index 3 (96% over 28bp)
TGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCAC30.14506769825918764No Hit
ATACTCACGCCACCCACCGGAGAATGGGAAGCCGAACATACTGAACTGCT30.14506769825918764No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA30.14506769825918764RNA PCR Primer, Index 17 (96% over 28bp)
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT30.14506769825918764No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT30.14506769825918764No Hit
AACATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAAT30.14506769825918764No Hit
GTCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCAGGATCTCCGGC30.14506769825918764No Hit
ATCCAGAAAACCCCTCAAATTCAAGTATACTCACGCCACCCACCGGAGAA30.14506769825918764No Hit
ACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTT30.14506769825918764No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.14506769825918764No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT30.14506769825918764No Hit
TTCCAGTACTCGGCGTCTGGCCGCCCCAGCTCGGTCACCGCGCGGTGCTC30.14506769825918764No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)