##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939877_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2068 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.40909090909091 31.0 30.0 31.0 27.0 34.0 2 30.584622823984525 31.0 30.0 31.0 27.0 34.0 3 30.629110251450676 31.0 30.0 31.0 27.0 34.0 4 34.64458413926499 35.0 35.0 37.0 32.0 37.0 5 34.30754352030948 35.0 35.0 37.0 30.0 37.0 6 34.20261121856866 35.0 33.0 37.0 30.0 37.0 7 33.9830754352031 35.0 33.0 37.0 30.0 37.0 8 33.7321083172147 35.0 33.0 37.0 28.0 37.0 9 35.31382978723404 37.0 34.0 39.0 30.0 39.0 10-11 35.135880077369436 37.0 33.5 39.0 29.5 39.0 12-13 35.093326885880074 37.0 34.0 39.0 29.0 39.0 14-15 35.74419729206963 37.5 33.5 40.0 29.5 41.0 16-17 35.57543520309478 37.0 33.0 40.0 28.0 41.0 18-19 35.75265957446808 38.0 34.0 40.0 28.0 41.0 20-21 35.54811411992263 38.0 34.0 40.0 27.0 41.0 22-23 35.42311411992263 37.5 33.5 40.0 27.0 41.0 24-25 35.08486460348162 37.0 33.0 39.5 27.0 40.0 26-27 34.62862669245648 37.0 32.5 39.0 25.5 40.0 28-29 34.169729206963254 37.0 32.5 39.0 24.5 40.0 30-31 33.842843326885884 36.0 31.5 39.0 24.5 40.0 32-33 33.90449709864603 36.0 32.0 39.0 24.0 40.0 34-35 33.969777562862674 36.5 32.0 39.0 24.5 40.0 36-37 33.946083172147 37.0 32.0 39.0 24.0 40.0 38-39 33.58679883945841 36.0 31.5 39.0 24.0 40.0 40-41 33.1791586073501 36.0 31.0 39.0 22.5 40.0 42-43 32.977756286266924 36.0 31.0 39.0 21.5 40.0 44-45 32.61895551257253 36.0 31.0 39.0 20.5 40.0 46-47 32.06237911025145 35.5 30.0 39.0 16.5 40.0 48-49 31.7137330754352 35.0 30.0 39.0 13.5 40.0 50-51 30.968810444874272 35.0 29.0 38.5 8.5 40.0 52-53 30.593810444874276 35.0 28.5 38.0 4.5 40.0 54-55 30.101547388781434 34.0 27.5 38.0 2.0 40.0 56-57 28.875725338491296 33.5 25.0 38.0 2.0 40.0 58-59 25.563346228239844 31.0 13.0 37.0 2.0 39.0 60-61 25.592117988394584 31.0 15.5 36.0 2.0 39.0 62-63 26.516924564796906 32.0 21.0 36.5 2.0 39.0 64-65 25.783607350096712 32.0 17.5 36.0 2.0 39.0 66-67 21.070116054158607 22.5 5.0 33.5 2.0 37.5 68-69 14.50169245647969 10.5 2.0 26.0 2.0 33.5 70-71 13.545938104448743 10.5 2.0 23.0 2.0 31.5 72-73 15.131286266924565 12.0 2.0 27.0 2.0 33.0 74-75 15.900628626692455 14.0 2.0 28.5 2.0 34.0 76-77 16.9997582205029 19.0 2.0 29.0 2.0 33.5 78-79 19.571083172147002 24.5 2.0 31.5 2.0 34.5 80-81 21.177949709864606 28.5 2.0 33.5 2.0 35.0 82-83 21.514023210831724 29.0 2.0 34.0 2.0 35.0 84-85 21.597437137330754 29.5 2.0 34.0 2.0 35.0 86-87 21.09284332688588 29.0 2.0 34.0 2.0 35.0 88-89 20.66247582205029 29.0 2.0 34.0 2.0 35.0 90-91 19.52151837524178 27.0 2.0 33.5 2.0 35.0 92-93 17.82809477756286 22.0 2.0 33.0 2.0 34.5 94-95 14.470502901353965 2.0 2.0 31.0 2.0 34.0 96-97 13.5178916827853 2.0 2.0 30.0 2.0 34.0 98-99 12.523694390715669 2.0 2.0 29.5 2.0 34.0 100-101 11.777804642166345 2.0 2.0 28.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -0.12056737588652666 1102 2 0.6400709219858243 1102 3 0.2996453900709355 1102 4 0.35106382978723616 1102 5 0.7482269503546135 1102 6 1.1276595744680762 1102 7 -7.0567375886524815 1102 8 1.5487588652482316 1102 9 2.2171985815602966 1102 10-11 -0.24556737588653021 1102 12-13 1.0828900709219909 1102 14-15 -0.6817375886524815 1102 16-17 4.494237588652474 1102 18-19 2.953457446808514 1102 20-21 -0.21631205673757847 1102 22-23 2.5172872340425414 1102 24-25 0.5274822695035439 1102 26-27 2.4640957446808542 1102 28-29 3.3847517730496435 1102 30-31 5.0403368794326155 1102 32-33 -0.5199468085106389 1102 34-35 -0.7553191489361737 1102 36-37 1.097960992907801 1102 38-39 -0.21453900709219909 1102 40-41 5.364804964539012 1102 42-43 2.6511524822695023 1102 44-45 7.5044326241134875 1102 46-47 3.636081560283696 1102 48-49 4.736258865248235 1102 50-51 -13.615248226950346 1102 52-53 2.355496453900706 1102 54-55 -7.851950354609929 1102 56-57 -1.3998226950354642 1102 58-59 10.78945035460993 1102 60-61 -0.5562943262411331 1102 62-63 -4.756648936170208 1102 64-65 -5.851950354609929 1102 66-67 15.30540780141844 1102 68-69 -12.270833333333332 1102 70-71 -10.11214539007092 1102 72-73 -12.25088652482269 1102 74-75 -12.699024822695032 1102 76-77 -13.326684397163122 1102 78-79 -16.109042553191486 1102 80-81 -17.32801418439716 1102 82-83 -17.455230496453904 1102 84-85 -17.21276595744681 1102 86-87 -16.707890070921987 1102 88-89 -15.865691489361701 1102 90-91 -14.894060283687946 1102 92-93 -13.808510638297872 1102 94-95 -10.932180851063828 1102 96-97 -9.773049645390069 1102 98-99 -8.192375886524824 1102 100-101 -8.029255319148934 1103 1 -0.12056737588652666 1103 2 0.6400709219858243 1103 3 0.2996453900709355 1103 4 -2.648936170212764 1103 5 0.7482269503546135 1103 6 1.1276595744680762 1103 7 -2.0567375886524815 1103 8 -1.4512411347517684 1103 9 0.2171985815602966 1103 10-11 -5.24556737588653 1103 12-13 -11.41710992907801 1103 14-15 -1.1817375886524815 1103 16-17 -2.5057624113475256 1103 18-19 -0.546542553191486 1103 20-21 -0.7163120567375785 1103 22-23 -6.982712765957459 1103 24-25 -4.472517730496456 1103 26-27 -4.035904255319146 1103 28-29 -7.1152482269503565 1103 30-31 -20.959663120567384 1103 32-33 -9.519946808510639 1103 34-35 -4.255319148936174 1103 36-37 -2.402039007092199 1103 38-39 -6.214539007092199 1103 40-41 0.3648049645390117 1103 42-43 1.1511524822695023 1103 44-45 0.5044326241134875 1103 46-47 -0.3639184397163042 1103 48-49 -12.763741134751765 1103 50-51 -9.615248226950346 1103 52-53 -17.144503546099294 1103 54-55 -0.8519503546099294 1103 56-57 5.600177304964536 1103 58-59 8.78945035460993 1103 60-61 -5.056294326241133 1103 62-63 5.743351063829792 1103 64-65 6.648049645390071 1103 66-67 -17.69459219858156 1103 68-69 -12.270833333333332 1103 70-71 -10.11214539007092 1103 72-73 -12.25088652482269 1103 74-75 -12.699024822695032 1103 76-77 -13.326684397163122 1103 78-79 -16.109042553191486 1103 80-81 -17.32801418439716 1103 82-83 -17.455230496453904 1103 84-85 -17.21276595744681 1103 86-87 -16.707890070921987 1103 88-89 -15.865691489361701 1103 90-91 -14.894060283687946 1103 92-93 -13.808510638297872 1103 94-95 -10.932180851063828 1103 96-97 -9.773049645390069 1103 98-99 -8.192375886524824 1103 100-101 -8.029255319148934 1104 1 -0.6205673758865267 1104 2 0.6400709219858243 1104 3 -1.7003546099290645 1104 4 -1.1489361702127638 1104 5 0.7482269503546135 1104 6 2.127659574468076 1104 7 2.9432624113475185 1104 8 2.5487588652482316 1104 9 -4.782801418439703 1104 10-11 0.2544326241134698 1104 12-13 1.5828900709219909 1104 14-15 -2.4317375886524815 1104 16-17 -6.255762411347526 1104 18-19 -2.046542553191486 1104 20-21 2.7836879432624215 1104 22-23 -1.4827127659574586 1104 24-25 -0.47251773049645607 1104 26-27 2.9640957446808542 1104 28-29 0.8847517730496435 1104 30-31 -2.2096631205673845 1104 32-33 -2.519946808510639 1104 34-35 -2.2553191489361737 1104 36-37 3.097960992907801 1104 38-39 2.035460992907801 1104 40-41 5.114804964539012 1104 42-43 -0.09884751773049771 1104 44-45 -1.2455673758865125 1104 46-47 -2.613918439716304 1104 48-49 -10.263741134751765 1104 50-51 -12.115248226950346 1104 52-53 -9.894503546099294 1104 54-55 -9.35195035460993 1104 56-57 -7.399822695035464 1104 58-59 -6.960549645390071 1104 60-61 -10.556294326241133 1104 62-63 -8.006648936170208 1104 64-65 -4.351950354609929 1104 66-67 -3.19459219858156 1104 68-69 -3.520833333333332 1104 70-71 -5.1121453900709195 1104 72-73 -8.00088652482269 1104 74-75 -8.199024822695032 1104 76-77 -5.326684397163122 1104 78-79 -8.859042553191486 1104 80-81 -2.0780141843971585 1104 82-83 -2.205230496453904 1104 84-85 -2.462765957446809 1104 86-87 -1.9578900709219873 1104 88-89 -0.6156914893617014 1104 90-91 -1.6440602836879457 1104 92-93 2.4414893617021285 1104 94-95 -2.932180851063828 1104 96-97 -9.773049645390069 1104 98-99 -8.192375886524824 1104 100-101 -8.029255319148934 1105 1 -2.1205673758865267 1105 2 0.14007092198582427 1105 3 0.2996453900709355 1105 4 -0.6489361702127638 1105 5 0.7482269503546135 1105 6 -1.3723404255319238 1105 7 1.9432624113475185 1105 8 -0.4512411347517684 1105 9 -2.2828014184397034 1105 10-11 1.7544326241134698 1105 12-13 1.0828900709219909 1105 14-15 -5.6817375886524815 1105 16-17 -6.755762411347526 1105 18-19 -0.546542553191486 1105 20-21 -5.2163120567375785 1105 22-23 -9.732712765957459 1105 24-25 -4.222517730496456 1105 26-27 -10.035904255319146 1105 28-29 -11.115248226950357 1105 30-31 -2.4596631205673845 1105 32-33 -1.769946808510639 1105 34-35 -1.7553191489361737 1105 36-37 -0.6520390070921991 1105 38-39 -3.714539007092199 1105 40-41 -8.385195035460988 1105 42-43 -3.8488475177304977 1105 44-45 -4.2455673758865125 1105 46-47 2.886081560283696 1105 48-49 0.9862588652482351 1105 50-51 -12.615248226950346 1105 52-53 -9.894503546099294 1105 54-55 -12.10195035460993 1105 56-57 -7.399822695035464 1105 58-59 -4.960549645390071 1105 60-61 -10.306294326241133 1105 62-63 -7.256648936170208 1105 64-65 -5.351950354609929 1105 66-67 -6.69459219858156 1105 68-69 2.979166666666668 1105 70-71 -5.8621453900709195 1105 72-73 -8.25088652482269 1105 74-75 -0.9490248226950317 1105 76-77 0.6733156028368779 1105 78-79 -11.859042553191486 1105 80-81 -11.828014184397158 1105 82-83 -3.705230496453904 1105 84-85 -5.462765957446809 1105 86-87 -4.707890070921987 1105 88-89 -0.6156914893617014 1105 90-91 -14.894060283687946 1105 92-93 -13.808510638297872 1105 94-95 -10.932180851063828 1105 96-97 -9.773049645390069 1105 98-99 -8.192375886524824 1105 100-101 -8.029255319148934 1106 1 -14.120567375886527 1106 2 -2.3599290780141757 1106 3 -4.7003546099290645 1106 4 0.35106382978723616 1106 5 0.7482269503546135 1106 6 1.1276595744680762 1106 7 -7.0567375886524815 1106 8 -17.45124113475177 1106 9 -11.782801418439703 1106 10-11 -17.24556737588653 1106 12-13 -4.917109929078009 1106 14-15 0.8182624113475185 1106 16-17 -5.005762411347526 1106 18-19 -24.546542553191486 1106 20-21 -10.216312056737578 1106 22-23 -1.9827127659574586 1106 24-25 -18.972517730496456 1106 26-27 -7.535904255319146 1106 28-29 -2.1152482269503565 1106 30-31 1.5403368794326155 1106 32-33 2.480053191489361 1106 34-35 -4.255319148936174 1106 36-37 -4.402039007092199 1106 38-39 1.785460992907801 1106 40-41 -7.635195035460988 1106 42-43 2.6511524822695023 1106 44-45 -0.9955673758865125 1106 46-47 -6.363918439716304 1106 48-49 4.236258865248235 1106 50-51 -3.115248226950346 1106 52-53 0.8554964539007059 1106 54-55 5.148049645390071 1106 56-57 7.600177304964536 1106 58-59 -4.710549645390071 1106 60-61 -6.056294326241133 1106 62-63 2.2433510638297918 1106 64-65 11.14804964539007 1106 66-67 15.80540780141844 1106 68-69 4.229166666666668 1106 70-71 12.88785460992908 1106 72-73 2.7491134751773103 1106 74-75 -12.699024822695032 1106 76-77 -13.326684397163122 1106 78-79 -16.109042553191486 1106 80-81 -17.32801418439716 1106 82-83 -17.455230496453904 1106 84-85 -17.21276595744681 1106 86-87 -16.707890070921987 1106 88-89 -15.865691489361701 1106 90-91 -14.894060283687946 1106 92-93 -13.808510638297872 1106 94-95 -10.932180851063828 1106 96-97 -9.773049645390069 1106 98-99 -8.192375886524824 1106 100-101 -8.029255319148934 1107 1 0.8794326241134733 1107 2 3.6400709219858243 1107 3 3.2996453900709355 1107 4 2.351063829787236 1107 5 0.7482269503546135 1107 6 3.127659574468076 1107 7 3.9432624113475185 1107 8 3.5487588652482316 1107 9 4.217198581560297 1107 10-11 3.25443262411347 1107 12-13 3.582890070921991 1107 14-15 5.8182624113475185 1107 16-17 5.494237588652474 1107 18-19 4.453457446808514 1107 20-21 3.7836879432624215 1107 22-23 -0.9827127659574586 1107 24-25 3.527482269503544 1107 26-27 5.464095744680854 1107 28-29 2.3847517730496435 1107 30-31 4.5403368794326155 1107 32-33 5.480053191489361 1107 34-35 4.744680851063826 1107 36-37 3.097960992907801 1107 38-39 -4.214539007092199 1107 40-41 -6.135195035460988 1107 42-43 -0.3488475177304977 1107 44-45 -1.9955673758865125 1107 46-47 2.136081560283696 1107 48-49 -28.763741134751765 1107 50-51 -28.115248226950346 1107 52-53 -27.644503546099294 1107 54-55 -27.85195035460993 1107 56-57 -25.399822695035464 1107 58-59 -22.21054964539007 1107 60-61 -22.056294326241133 1107 62-63 -22.75664893617021 1107 64-65 -21.85195035460993 1107 66-67 -17.69459219858156 1107 68-69 -12.270833333333332 1107 70-71 -10.11214539007092 1107 72-73 -12.25088652482269 1107 74-75 -12.699024822695032 1107 76-77 -13.326684397163122 1107 78-79 -16.109042553191486 1107 80-81 -17.32801418439716 1107 82-83 -17.455230496453904 1107 84-85 -17.21276595744681 1107 86-87 -16.707890070921987 1107 88-89 -15.865691489361701 1107 90-91 -14.894060283687946 1107 92-93 -13.808510638297872 1107 94-95 -10.932180851063828 1107 96-97 -9.773049645390069 1107 98-99 -8.192375886524824 1107 100-101 -8.029255319148934 1108 1 -0.7872340425531945 1108 2 0.3067375886524921 1108 3 -0.03368794326239666 1108 4 0.35106382978723616 1108 5 -4.585106382978719 1108 6 -3.5390070921985917 1108 7 -0.39007092198581717 1108 8 -0.7845744680851041 1108 9 -1.1161347517730391 1108 10-11 -0.07890070921986592 1108 12-13 0.9162234042553266 1108 14-15 -2.348404255319153 1108 16-17 -3.67242907801419 1108 18-19 -3.046542553191486 1108 20-21 -2.049645390070914 1108 22-23 2.35062056737587 1108 24-25 0.027482269503543932 1108 26-27 -1.5359042553191458 1108 28-29 -0.4485815602836851 1108 30-31 -2.6263297872340488 1108 32-33 -2.519946808510639 1108 34-35 -1.7553191489361737 1108 36-37 -0.7353723404255348 1108 38-39 -1.214539007092199 1108 40-41 -2.968528368794324 1108 42-43 -14.18218085106383 1108 44-45 -11.495567375886512 1108 46-47 -9.530585106382972 1108 48-49 -11.763741134751765 1108 50-51 -5.948581560283678 1108 52-53 -8.144503546099294 1108 54-55 -6.018617021276594 1108 56-57 -13.733156028368796 1108 58-59 -10.543882978723403 1108 60-61 -17.7229609929078 1108 62-63 -12.923315602836876 1108 64-65 -14.185283687943263 1108 66-67 -7.527925531914894 1108 68-69 -8.604166666666664 1108 70-71 -5.7788120567375865 1108 72-73 -10.25088652482269 1108 74-75 -9.865691489361698 1108 76-77 -8.993351063829788 1108 78-79 -8.775709219858154 1108 80-81 -7.161347517730492 1108 82-83 -8.788563829787236 1108 84-85 -9.046099290780143 1108 86-87 -6.707890070921987 1108 88-89 -5.5323581560283674 1108 90-91 -9.727393617021278 1108 92-93 -13.808510638297872 1108 94-95 -10.932180851063828 1108 96-97 -9.773049645390069 1108 98-99 -8.192375886524824 1108 100-101 -8.029255319148934 1109 1 2.3794326241134733 1109 2 0.6400709219858243 1109 3 0.2996453900709355 1109 4 1.3510638297872362 1109 5 1.7482269503546135 1109 6 2.627659574468076 1109 7 3.9432624113475185 1109 8 2.5487588652482316 1109 9 -1.2828014184397034 1109 10-11 3.75443262411347 1109 12-13 3.582890070921991 1109 14-15 3.0682624113475185 1109 16-17 1.4942375886524744 1109 18-19 3.703457446808514 1109 20-21 3.0336879432624215 1109 22-23 -0.9827127659574586 1109 24-25 3.027482269503544 1109 26-27 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11.300975177304968 2314 76-77 15.673315602836878 2314 78-79 7.640957446808514 2314 80-81 -1.0780141843971585 2314 82-83 -2.705230496453904 2314 84-85 -8.462765957446809 2314 86-87 -11.207890070921987 2314 88-89 -6.365691489361701 2314 90-91 -7.644060283687946 2314 92-93 0.9414893617021285 2314 94-95 4.067819148936172 2314 96-97 -9.773049645390069 2314 98-99 -8.192375886524824 2314 100-101 -8.029255319148934 2315 1 0.8794326241134733 2315 2 -8.359929078014176 2315 3 -4.7003546099290645 2315 4 0.35106382978723616 2315 5 0.7482269503546135 2315 6 -6.872340425531924 2315 7 1.9432624113475185 2315 8 -2.4512411347517684 2315 9 -0.7828014184397034 2315 10-11 1.2544326241134698 2315 12-13 -4.917109929078009 2315 14-15 -11.181737588652481 2315 16-17 -4.005762411347526 2315 18-19 -8.546542553191486 2315 20-21 -7.2163120567375785 2315 22-23 -15.982712765957459 2315 24-25 -10.472517730496456 2315 26-27 -5.535904255319146 2315 28-29 -17.615248226950357 2315 30-31 -3.4596631205673845 2315 32-33 -17.51994680851064 2315 34-35 -31.755319148936174 2315 36-37 -32.4020390070922 2315 38-39 -31.2145390070922 2315 40-41 -30.63519503546099 2315 42-43 -30.348847517730498 2315 44-45 -29.995567375886512 2315 46-47 -28.863918439716304 2315 48-49 -28.763741134751765 2315 50-51 -28.115248226950346 2315 52-53 -27.644503546099294 2315 54-55 -27.85195035460993 2315 56-57 -25.399822695035464 2315 58-59 -22.21054964539007 2315 60-61 -22.056294326241133 2315 62-63 -22.75664893617021 2315 64-65 -21.85195035460993 2315 66-67 -17.69459219858156 2315 68-69 -12.270833333333332 2315 70-71 -10.11214539007092 2315 72-73 -12.25088652482269 2315 74-75 -12.699024822695032 2315 76-77 -13.326684397163122 2315 78-79 -16.109042553191486 2315 80-81 -17.32801418439716 2315 82-83 -17.455230496453904 2315 84-85 -17.21276595744681 2315 86-87 -16.707890070921987 2315 88-89 -15.865691489361701 2315 90-91 -14.894060283687946 2315 92-93 -13.808510638297872 2315 94-95 -10.932180851063828 2315 96-97 -9.773049645390069 2315 98-99 -8.192375886524824 2315 100-101 -8.029255319148934 2316 1 -2.1205673758865267 2316 2 -1.3599290780141757 2316 3 -4.7003546099290645 2316 4 -1.1489361702127638 2316 5 -7.2517730496453865 2316 6 -2.872340425531924 2316 7 -2.5567375886524815 2316 8 -7.451241134751768 2316 9 -2.7828014184397034 2316 10-11 3.25443262411347 2316 12-13 -1.1671099290780091 2316 14-15 -0.9317375886524815 2316 16-17 -10.255762411347526 2316 18-19 -0.29654255319148604 2316 20-21 -5.2163120567375785 2316 22-23 1.0172872340425414 2316 24-25 -1.722517730496456 2316 26-27 -14.035904255319146 2316 28-29 -11.115248226950357 2316 30-31 -1.7096631205673845 2316 32-33 0.2300531914893611 2316 34-35 -2.0053191489361737 2316 36-37 -2.652039007092199 2316 38-39 -0.4645390070921991 2316 40-41 1.3648049645390117 2316 42-43 -1.0988475177304977 2316 44-45 -6.2455673758865125 2316 46-47 -18.863918439716304 2316 48-49 -15.513741134751765 2316 50-51 -19.365248226950346 2316 52-53 -13.644503546099294 2316 54-55 -14.35195035460993 2316 56-57 -11.649822695035464 2316 58-59 -12.71054964539007 2316 60-61 -13.556294326241133 2316 62-63 -10.506648936170208 2316 64-65 -10.10195035460993 2316 66-67 -5.19459219858156 2316 68-69 0.22916666666666785 2316 70-71 -5.8621453900709195 2316 72-73 -5.75088652482269 2316 74-75 2.0509751773049683 2316 76-77 -5.326684397163122 2316 78-79 -2.609042553191486 2316 80-81 -10.828014184397158 2316 82-83 -10.955230496453904 2316 84-85 -8.462765957446809 2316 86-87 -3.4578900709219873 2316 88-89 -0.36569148936170137 2316 90-91 -10.894060283687946 2316 92-93 -4.5585106382978715 2316 94-95 2.067819148936172 2316 96-97 2.226950354609931 2316 98-99 4.5576241134751765 2316 100-101 6.970744680851066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 4.0 8 4.0 9 10.0 10 12.0 11 10.0 12 18.0 13 31.0 14 33.0 15 54.0 16 46.0 17 54.0 18 70.0 19 63.0 20 72.0 21 61.0 22 49.0 23 41.0 24 49.0 25 58.0 26 73.0 27 100.0 28 107.0 29 152.0 30 177.0 31 141.0 32 138.0 33 124.0 34 102.0 35 110.0 36 58.0 37 40.0 38 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5705996131528 19.97098646034816 16.054158607350097 23.404255319148938 2 15.81237911025145 20.502901353965182 37.33075435203095 26.353965183752422 3 18.32688588007737 25.09671179883946 30.174081237911025 26.402321083172147 4 12.475822050290136 17.06963249516441 33.849129593810446 36.60541586073501 5 13.062409288824384 37.0585389453314 32.365747460087086 17.513304305757135 6 32.15667311411992 36.07350096711799 15.667311411992262 16.10251450676983 7 26.402321083172147 35.20309477756286 20.6963249516441 17.69825918762089 8 27.36943907156673 33.26885880077369 18.810444874274662 20.551257253384914 9 26.063829787234045 14.748549323017407 18.32688588007737 40.86073500967118 10-11 23.815280464216634 25.94294003868472 24.709864603481623 25.53191489361702 12-13 25.77369439071567 24.129593810444874 26.716634429400386 23.38007736943907 14-15 22.775628626692455 26.25725338491296 24.68568665377176 26.281431334622823 16-17 21.953578336557058 29.908123791102515 25.94294003868472 22.195357833655706 18-19 21.252417794970988 26.136363636363637 27.901353965183752 24.709864603481623 20-21 23.452611218568663 23.984526112185687 30.270793036750483 22.292069632495163 22-23 22.219535783365572 26.64410058027079 27.34526112185687 23.791102514506772 24-25 22.920696324951646 26.64410058027079 28.264023210831724 22.17117988394584 26-27 21.155705996131527 27.949709864603484 27.58704061895551 23.30754352030948 28-29 20.623791102514506 27.20019342359768 28.892649903288202 23.283365570599614 30-31 22.00193423597679 24.492263056092845 28.965183752417794 24.54061895551257 32-33 22.147001934235977 25.024177949709863 29.110251450676984 23.718568665377177 34-35 22.38878143133462 27.248549323017407 29.738878143133462 20.623791102514506 36-37 23.549323017408124 25.4110251450677 27.151837524177953 23.88781431334623 38-39 21.639264990328822 26.20889748549323 27.20019342359768 24.95164410058027 40-41 21.300773694390713 25.09671179883946 29.78723404255319 23.815280464216634 42-43 22.872340425531913 25.241779497098648 29.013539651837522 22.872340425531913 44-45 21.27659574468085 25.604448742746616 28.892649903288202 24.22630560928433 46-47 22.89651837524178 25.507736943907155 27.41779497098646 24.177949709864606 48-49 21.445841392649903 26.1605415860735 29.47292069632495 22.920696324951646 50-51 22.292069632495163 27.68375241779497 27.490328820116055 22.53384912959381 52-53 21.155705996131527 28.433268858800776 27.466150870406192 22.944874274661508 54-55 21.034816247582206 30.609284332688585 25.749516441005802 22.606382978723406 56-57 20.581113801452787 29.273607748184023 26.610169491525426 23.53510895883777 58-59 20.380302291565087 27.766942954656265 28.668941979522184 23.18381277425646 60-61 20.90710647586709 28.64419112296871 27.06766917293233 23.38103322823187 62-63 21.663442940038685 28.022243713733076 27.538684719535784 22.775628626692455 64-65 22.106537530266344 26.803874092009682 27.627118644067792 23.462469733656174 66-67 23.428290766208253 24.386051080550097 27.971512770137526 24.21414538310413 68-69 23.749066467513067 24.22205626089121 28.553647000248944 23.475230271346774 70-71 20.568603213844252 25.06798516687268 29.690976514215077 24.672435105067986 72-73 21.095484826054776 25.857389587959535 29.583024919812484 23.464100666173206 74-75 22.74403737398574 26.28473075977379 27.51413818539464 23.457093680845833 76-77 19.476388549057987 26.963542941032543 28.65182285294837 24.908245656961096 78-79 21.68528411850413 26.93054881010199 27.659057795046138 23.72510927634774 80-81 21.760154738878143 26.547388781431337 27.006769825918763 24.68568665377176 82-83 21.59090909090909 27.055125725338492 28.60251450676983 22.751450676982593 84-85 21.39748549323017 26.740812379110253 26.4990328820116 25.362669245647968 86-87 22.195357833655706 26.740812379110253 26.95841392649903 24.10541586073501 88-89 21.8568665377176 27.36943907156673 27.538684719535784 23.235009671179885 90-91 20.377998546159436 27.186818512236492 28.301429609886114 24.133753331717955 92-93 20.348837209302324 28.0765503875969 26.332364341085274 25.242248062015506 94-95 20.392156862745097 25.857843137254903 28.480392156862745 25.269607843137255 96-97 20.460671404067632 25.777995589316344 27.860818426856166 25.900514579759864 98-99 19.936476911800636 24.651844612753482 28.90300513071097 26.508673344734913 100-101 19.50509461426492 26.589034449296456 27.996118389131492 25.90975254730713 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 3.0 9 3.5 10 1.0 11 1.0 12 2.0 13 2.0 14 2.0 15 3.0 16 2.0 17 0.5 18 1.5 19 2.0 20 2.5 21 4.0 22 5.0 23 5.0 24 6.5 25 6.0 26 6.0 27 11.5 28 14.5 29 19.5 30 19.5 31 20.0 32 28.0 33 35.0 34 43.5 35 48.0 36 51.5 37 54.5 38 55.5 39 55.5 40 57.5 41 59.0 42 65.0 43 80.0 44 83.5 45 81.0 46 79.5 47 79.5 48 77.5 49 79.5 50 81.0 51 80.0 52 82.0 53 71.5 54 66.0 55 62.5 56 55.0 57 52.0 58 41.5 59 26.5 60 27.5 61 27.0 62 23.5 63 24.0 64 20.0 65 14.0 66 11.5 67 11.5 68 8.0 69 6.0 70 5.5 71 5.0 72 3.5 73 2.0 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.04835589941972921 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.14506769825918764 58-59 0.8220502901353964 60-61 0.3143133462282398 62-63 0.0 64-65 0.14506769825918764 66-67 1.5473887814313347 68-69 2.877176015473888 70-71 2.2001934235976788 72-73 2.006769825918762 74-75 1.6682785299806577 76-77 1.1847195357833655 78-79 0.43520309477756286 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.21760154738878143 92-93 0.19342359767891684 94-95 1.3539651837524178 96-97 1.3297872340425532 98-99 1.039651837524178 100-101 0.33849129593810445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2068.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.64990328820116 #Duplication Level Percentage of deduplicated Percentage of total 1 96.3465553235908 89.26499032882012 2 2.0876826722338206 3.8684719535783367 3 0.6784968684759917 1.885880077369439 4 0.2609603340292276 0.9671179883945842 5 0.31315240083507306 1.4506769825918762 6 0.10438413361169101 0.5802707930367506 7 0.052192066805845504 0.33849129593810445 8 0.0 0.0 9 0.0 0.0 >10 0.15657620041753653 1.6441005802707929 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 12 0.5802707930367506 RNA PCR Primer, Index 17 (96% over 28bp) TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 12 0.5802707930367506 RNA PCR Primer, Index 31 (96% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.4835589941972921 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 7 0.33849129593810445 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 6 0.2901353965183753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.2901353965183753 No Hit ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC 5 0.24177949709864605 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 5 0.24177949709864605 TruSeq Adapter, Index 3 (96% over 28bp) ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA 5 0.24177949709864605 No Hit TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATA 5 0.24177949709864605 No Hit ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA 5 0.24177949709864605 No Hit GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA 5 0.24177949709864605 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.19342359767891684 No Hit TCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATAC 4 0.19342359767891684 No Hit GTAGAGATGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCT 4 0.19342359767891684 No Hit GTTCAAGCCGCCGCAGGGAGGTGTGGGTCTCCGGCCCCTCGTAGTTGTGT 4 0.19342359767891684 No Hit GATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCACACTGA 4 0.19342359767891684 No Hit TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA 3 0.14506769825918764 TruSeq Adapter, Index 3 (96% over 28bp) TGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCAC 3 0.14506769825918764 No Hit ATACTCACGCCACCCACCGGAGAATGGGAAGCCGAACATACTGAACTGCT 3 0.14506769825918764 No Hit CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 3 0.14506769825918764 RNA PCR Primer, Index 17 (96% over 28bp) ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT 3 0.14506769825918764 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 3 0.14506769825918764 No Hit AACATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAAT 3 0.14506769825918764 No Hit GTCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCAGGATCTCCGGC 3 0.14506769825918764 No Hit ATCCAGAAAACCCCTCAAATTCAAGTATACTCACGCCACCCACCGGAGAA 3 0.14506769825918764 No Hit ACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTT 3 0.14506769825918764 No Hit GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA 3 0.14506769825918764 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 3 0.14506769825918764 No Hit TTCCAGTACTCGGCGTCTGGCCGCCCCAGCTCGGTCACCGCGCGGTGCTC 3 0.14506769825918764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0725338491295938 0.0 34-35 0.0 0.0 0.0 0.09671179883945841 0.0 36-37 0.0 0.0 0.0 0.1450676982591876 0.0 38-39 0.0 0.0 0.0 0.2901353965183753 0.0 40-41 0.0 0.0 0.0 0.4835589941972921 0.0 42-43 0.0 0.0 0.0 0.5560928433268859 0.0 44-45 0.0 0.0 0.0 0.6769825918762089 0.0 46-47 0.0 0.0 0.0 0.8462282398452611 0.0 48-49 0.0 0.0 0.0 1.0638297872340425 0.0 50-51 0.0 0.0 0.0 1.2330754352030948 0.0 52-53 0.0 0.0 0.0 1.3781431334622825 0.0 54-55 0.0 0.0 0.0 1.4264990328820115 0.0 56-57 0.0 0.0 0.0 1.5473887814313345 0.0 58-59 0.0 0.0 0.0 1.5473887814313345 0.0 60-61 0.0 0.0 0.0 1.5473887814313345 0.0 62-63 0.0 0.0 0.0 1.5957446808510638 0.0 64-65 0.0 0.0 0.0 1.6924564796905222 0.0 66-67 0.0 0.0 0.0 1.7408123791102514 0.0 68-69 0.0 0.0 0.0 1.7408123791102514 0.0 70-71 0.0 0.0 0.0 1.764990328820116 0.0 72-73 0.0 0.0 0.0 1.8617021276595747 0.0 74-75 0.0 0.0 0.0 2.0309477756286265 0.0 76-77 0.0 0.0 0.0 2.1518375241779495 0.0 78-79 0.0 0.0 0.0 2.3452611218568666 0.0 80-81 0.0 0.0 0.0 2.9980657640232105 0.0 82-83 0.0 0.0 0.0 3.36073500967118 0.0 84-85 0.0 0.0 0.0 3.7475822050290137 0.0 86-87 0.0 0.0 0.0 3.8201160541586074 0.0 88-89 0.0 0.0 0.0 3.965183752417795 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE