FastQCFastQC Report
Fri 27 May 2016
SRR939876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG150.6105006105006106RNA PCR Primer, Index 17 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.3663003663003663No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC80.3256003256003256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.2849002849002849No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT70.2849002849002849No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC60.2442002442002442No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA60.2442002442002442RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG50.20350020350020348RNA PCR Primer, Index 33 (96% over 29bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50.20350020350020348No Hit
GCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAG50.20350020350020348No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG50.20350020350020348RNA PCR Primer, Index 31 (96% over 29bp)
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA40.1628001628001628No Hit
CCGCATGACGGTCCCCAGTTCCTTGGTTGTGATGGTGCCGTCACCATCTT40.1628001628001628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1628001628001628No Hit
GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC40.1628001628001628No Hit
ACTTAGTGGTGACGTAGGAGACAGGGAAGGACAGGTCCTCTTGGCAGGGG30.1221001221001221No Hit
ATGTTATATTGATGTGTTTTTCCATTGACAGCCCTGATAGTTTAGAAAAC30.1221001221001221No Hit
AGCAAGGACTGGTCTTTCTATATCCTGGCTCACACTGAATTCACCCCCAC30.1221001221001221No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT30.1221001221001221No Hit
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA30.1221001221001221No Hit
GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT30.1221001221001221No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA30.1221001221001221No Hit
GTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCACA30.1221001221001221No Hit
ACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGA30.1221001221001221No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)