FastQCFastQC Report
Fri 27 May 2016
SRR939875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938997
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86720.2950666502891973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.23171170300616165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.14089840853869534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.051.8875731
GTATCAA96450.041.7076451
ATCAACG122250.033.8379823
TATCAAC124150.033.663452
TCAACGC125100.033.257834
CAACGCA125850.033.0224955
AACGCAG130300.031.968626
ACGCAGA148600.027.7124377
CGCAGAG150150.027.3627018
TACATGG106600.026.4782432
GTACATG108450.025.3262581
ACATGGG109200.024.8925173
GCAGAGT157400.024.8062739
TGGTATC24950.023.5765652
GTGGTAT26800.022.6567291
GAGTACT95550.022.4651812-13
CAGAGTA151650.021.5687510-11
CATGGGG60800.021.1454684
AGAGTAC142950.020.97326510-11
GTACTTT103450.020.75244514-15
Produced by FastQC (version 0.11.5)