##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939875_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2938997 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.849858982503214 31.0 30.0 33.0 16.0 34.0 2 29.682632544368026 31.0 30.0 33.0 25.0 34.0 3 29.936239131921536 31.0 30.0 34.0 25.0 34.0 4 33.70108986160925 35.0 33.0 37.0 28.0 37.0 5 33.657851300971046 35.0 33.0 37.0 28.0 37.0 6 33.6900197584414 35.0 33.0 37.0 28.0 37.0 7 33.61087881341832 35.0 33.0 37.0 28.0 37.0 8 33.466578904299666 35.0 33.0 37.0 27.0 37.0 9 34.73869044439311 37.0 34.0 39.0 27.0 39.0 10-11 34.54959021734285 37.0 33.5 39.0 27.0 39.0 12-13 34.42846215902908 37.0 33.0 39.0 27.0 39.0 14-15 35.082565752874196 38.0 33.0 40.0 26.5 41.0 16-17 34.89996519220673 38.0 33.0 40.0 25.5 41.0 18-19 34.74777228421805 38.0 33.0 40.0 25.0 41.0 20-21 34.94337081664255 38.0 34.0 40.0 25.0 41.0 22-23 34.84584775009978 38.0 33.5 40.0 25.0 41.0 24-25 34.62556222411932 38.0 33.0 40.0 24.5 41.0 26-27 34.49894317687293 38.0 33.0 40.0 24.0 41.0 28-29 34.53882872286022 38.0 33.0 40.0 24.0 41.0 30-31 34.30606717189572 38.0 33.0 40.0 23.5 41.0 32-33 33.946956393626806 38.0 32.5 40.0 22.0 41.0 34-35 33.74716629516804 38.0 32.0 40.0 20.5 41.0 36-37 33.516617573954655 38.0 32.0 40.0 19.0 41.0 38-39 33.271230627319454 37.0 31.0 40.0 18.0 41.0 40-41 33.02461877300317 37.0 31.0 40.0 16.5 41.0 42-43 32.6246850541188 37.0 31.0 40.0 15.0 41.0 44-45 32.254054359361376 37.0 30.0 40.0 11.5 41.0 46-47 31.852519413936115 36.5 30.0 39.0 9.0 41.0 48-49 31.472481938566116 36.0 30.0 39.0 8.5 40.5 50-51 30.84632903674281 35.5 28.5 38.5 7.5 40.0 52-53 31.01074550263236 36.0 29.0 39.0 6.5 40.0 54-55 31.095055728195707 36.0 29.0 39.0 2.0 40.0 56-57 30.776483609884597 35.5 28.5 39.0 2.0 40.0 58-59 30.355953408594836 35.0 28.0 39.0 2.0 40.0 60-61 29.932959101353283 35.0 28.0 38.5 2.0 40.0 62-63 29.47573100619021 34.0 27.0 38.0 2.0 40.0 64-65 28.923198968899932 34.0 26.0 38.0 2.0 40.0 66-67 28.320620266029533 34.0 26.0 37.5 2.0 39.5 68-69 27.743432198127458 33.0 24.5 36.5 2.0 39.0 70-71 27.165494214522845 32.5 23.0 36.0 2.0 39.0 72-73 26.509343323589647 32.0 22.0 35.5 2.0 38.5 74-75 26.148712468913715 32.0 21.0 35.0 2.0 38.0 76-77 25.57609568842704 31.5 19.5 35.0 2.0 37.0 78-79 24.90941688610094 31.0 17.0 35.0 2.0 36.5 80-81 24.170560738918752 31.0 9.5 34.0 2.0 36.0 82-83 23.404836241751863 30.0 4.5 34.0 2.0 35.5 84-85 22.59808448256327 29.5 2.0 34.0 2.0 35.0 86-87 21.636696294688292 29.0 2.0 34.0 2.0 35.0 88-89 20.60338714193992 28.0 2.0 33.5 2.0 35.0 90-91 19.16739945634514 25.5 2.0 32.5 2.0 34.5 92-93 17.59346011581502 21.5 2.0 32.0 2.0 34.0 94-95 17.084935268732835 19.5 2.0 31.0 2.0 34.0 96-97 16.778099467267232 19.0 2.0 31.5 2.0 34.0 98-99 15.894056373654006 4.5 2.0 31.5 2.0 34.0 100-101 14.287071916031216 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8158627057948813 1101 2 1.107064963821312 1101 3 1.1703132395932734 1101 4 1.1339745202282927 1101 5 1.2026420696837476 1101 6 1.2351146781865694 1101 7 1.0227255448121966 1101 8 1.3136185421036757 1101 9 1.2403941145482946 1101 10-11 1.5528118286691779 1101 12-13 1.466801068796869 1101 14-15 1.6706038252871878 1101 16-17 1.5987359237268635 1101 18-19 1.4980078413956264 1101 20-21 1.5158164350761325 1101 22-23 1.6689278498153826 1101 24-25 1.9406636183382417 1101 26-27 1.6314287475273161 1101 28-29 1.6852162759588367 1101 30-31 1.6441561541050547 1101 32-33 1.8318936910618149 1101 34-35 1.722807482675087 1101 36-37 1.5236395864148236 1101 38-39 1.5588540394745323 1101 40-41 1.3218283464276297 1101 42-43 1.3593689921224765 1101 44-45 1.5361655669368943 1101 46-47 1.5958554412660106 1101 48-49 1.4755166046809798 1101 50-51 1.3091072929130014 1101 52-53 1.2863389485623955 1101 54-55 1.265094528408607 1101 56-57 1.4942927480718815 1101 58-59 1.0585399952325112 1101 60-61 1.2428786489334023 1101 62-63 0.718171080579399 1101 64-65 1.1441391060022212 1101 66-67 1.3419545179663785 1101 68-69 0.833375986627253 1101 70-71 1.032214967190729 1101 72-73 0.7264302165059782 1101 74-75 0.6869010805046862 1101 76-77 0.677809202265788 1101 78-79 0.7295095535360261 1101 80-81 0.36293259663679933 1101 82-83 0.17306897493499918 1101 84-85 0.30502318609620716 1101 86-87 -0.33472872901324635 1101 88-89 -4.42766694794261 1101 90-91 -7.53783548505951 1101 92-93 -9.95432425958083 1101 94-95 -7.937171467159299 1101 96-97 -4.36295757818486 1101 98-99 -2.670344227752066 1101 100-101 -1.959084930251752 1102 1 1.7547072586437409 1102 2 1.1071123879782299 1102 3 1.0951706613615109 1102 4 1.075382972760579 1102 5 1.0162491585468842 1102 6 1.0797057159693964 1102 7 1.0764740874553738 1102 8 1.174719176913456 1102 9 1.4728640869449379 1102 10-11 1.3703951834889807 1102 12-13 1.3956788110675546 1102 14-15 1.6123979110954636 1102 16-17 1.5476187977905198 1102 18-19 1.5889486543005873 1102 20-21 1.6959373766603036 1102 22-23 1.5366542633485452 1102 24-25 1.6300856850979812 1102 26-27 1.6741534200947115 1102 28-29 1.4675354020963667 1102 30-31 1.641595873633591 1102 32-33 1.8932256059968537 1102 34-35 1.896132417772634 1102 36-37 1.9270193563178069 1102 38-39 1.8708700478731046 1102 40-41 1.8300223671399962 1102 42-43 1.8104492270567576 1102 44-45 1.784895036332962 1102 46-47 1.7906542842164157 1102 48-49 1.6191905840694467 1102 50-51 1.7482961701011526 1102 52-53 1.8966522799671424 1102 54-55 1.9194913592768437 1102 56-57 1.9426473419875627 1102 58-59 1.8886444506821398 1102 60-61 1.7874768252998479 1102 62-63 1.9074938062104323 1102 64-65 1.6603567948633078 1102 66-67 1.562511542145458 1102 68-69 1.505903322285704 1102 70-71 1.5150195918946707 1102 72-73 1.5136364020138053 1102 74-75 1.3339762455469888 1102 76-77 1.210922371030641 1102 78-79 1.1272202646812275 1102 80-81 1.2895440882089027 1102 82-83 0.9076213670219531 1102 84-85 0.8775554388909228 1102 86-87 0.7425213537918296 1102 88-89 0.33029313439570984 1102 90-91 -5.7139449669705655 1102 92-93 -10.140400052868415 1102 94-95 -8.03422275788261 1102 96-97 -4.614740788885147 1102 98-99 -2.9618985844471215 1102 100-101 -2.302986695353912 1103 1 2.1282890585978294 1103 2 1.27783125330048 1103 3 0.9954282573027555 1103 4 0.9723276749753538 1103 5 0.9911604076223455 1103 6 0.7534788736090903 1103 7 0.7105071119220838 1103 8 0.8832898578652859 1103 9 1.002771113055708 1103 10-11 0.9359675619647021 1103 12-13 0.8554911221131789 1103 14-15 1.0434012404200033 1103 16-17 1.0173126500171605 1103 18-19 0.8778934256719211 1103 20-21 0.9201936615696553 1103 22-23 1.0014747235879398 1103 24-25 0.8365688555291868 1103 26-27 1.0228910307697419 1103 28-29 0.8329986627940471 1103 30-31 0.9001649115039214 1103 32-33 0.8543727368332341 1103 34-35 0.9355739147523039 1103 36-37 0.9638220521485934 1103 38-39 1.0526193239363053 1103 40-41 0.9704162628224751 1103 42-43 0.9197085676072234 1103 44-45 0.8421169435631128 1103 46-47 0.8467111363076967 1103 48-49 0.8969003565296312 1103 50-51 0.8101204221802654 1103 52-53 0.8719966158973662 1103 54-55 0.8693302882427538 1103 56-57 0.8475689596429881 1103 58-59 0.9137032828092337 1103 60-61 0.8379546186855364 1103 62-63 0.9052631391487758 1103 64-65 0.8581318172778865 1103 66-67 0.6585844179598865 1103 68-69 0.7422151659114249 1103 70-71 0.7107636306968992 1103 72-73 0.803414485464689 1103 74-75 0.7326181651174153 1103 76-77 0.5987871473851776 1103 78-79 0.15300836749594993 1103 80-81 0.1593552000502747 1103 82-83 -0.15974025363516375 1103 84-85 -0.12336505460247338 1103 86-87 0.022155554802985478 1103 88-89 -0.7531843568591761 1103 90-91 -6.447254876174371 1103 92-93 -9.784811466742498 1103 94-95 -8.547581317012945 1103 96-97 -5.268069782357944 1103 98-99 -3.8602645521583643 1103 100-101 -3.1138198259587817 1104 1 2.0455749216802346 1104 2 1.3412339255752954 1104 3 1.091757528265319 1104 4 0.8088755775580694 1104 5 0.910679109565514 1104 6 0.8839135660785544 1104 7 0.9397493813654094 1104 8 0.9221039364015056 1104 9 1.0083306123027214 1104 10-11 1.04858909308264 1104 12-13 0.889002778485704 1104 14-15 1.073932046547391 1104 16-17 1.0119161044697336 1104 18-19 0.9571343268017429 1104 20-21 0.8863973545234245 1104 22-23 0.8563503563292727 1104 24-25 0.9070824043207537 1104 26-27 0.9305300763005846 1104 28-29 0.9173182875610237 1104 30-31 1.0147574360340528 1104 32-33 1.085316276071488 1104 34-35 1.0571666067971748 1104 36-37 0.957743008409004 1104 38-39 0.8720461000901665 1104 40-41 0.7404900439453925 1104 42-43 0.9078264233521551 1104 44-45 0.9338810230944645 1104 46-47 1.0058225701012233 1104 48-49 0.8297275658998409 1104 50-51 0.8070952054062381 1104 52-53 0.8053908460924788 1104 54-55 0.7357944997956345 1104 56-57 0.9502343514284632 1104 58-59 0.7571338023371403 1104 60-61 0.7394613391780638 1104 62-63 0.6368332001175041 1104 64-65 0.7040033251759574 1104 66-67 0.8171215747205167 1104 68-69 0.7726607634249127 1104 70-71 0.47265365312065555 1104 72-73 0.5208857342954012 1104 74-75 0.5105643099563046 1104 76-77 0.6552846048098964 1104 78-79 0.5292131053436648 1104 80-81 0.30198135855571095 1104 82-83 0.11795005804177805 1104 84-85 0.3341189568615057 1104 86-87 0.3408254048026542 1104 88-89 -0.0987071521329348 1104 90-91 -3.101155523345197 1104 92-93 -7.793336474537739 1104 94-95 -5.457148855309136 1104 96-97 -2.8931466981627114 1104 98-99 -1.9792825342856997 1104 100-101 -1.5581680277460492 1105 1 1.9016200587600132 1105 2 1.16906585650489 1105 3 0.9722410886059443 1105 4 0.8490079850025865 1105 5 0.9305201868815942 1105 6 0.7843837527821194 1105 7 0.7773650559430507 1105 8 0.8313081206201929 1105 9 0.8024919861169764 1105 10-11 0.7822564860882721 1105 12-13 0.7113021053740951 1105 14-15 0.7868110277884384 1105 16-17 0.8048105160191596 1105 18-19 0.7807803515197591 1105 20-21 0.7889582794559757 1105 22-23 0.7855451220480418 1105 24-25 0.9029008223924606 1105 26-27 0.605340124247391 1105 28-29 0.818899551390821 1105 30-31 1.0940710668077642 1105 32-33 1.1696776638078177 1105 34-35 1.0279055529798953 1105 36-37 1.0969221750668865 1105 38-39 0.9714127507956505 1105 40-41 0.7844638915238917 1105 42-43 0.9163573904939284 1105 44-45 0.7447797462707229 1105 46-47 0.9327591107167876 1105 48-49 0.8282332623520325 1105 50-51 0.8222144290602778 1105 52-53 0.7232875779890264 1105 54-55 0.7568809706953168 1105 56-57 0.8590918019576073 1105 58-59 0.6389460250820278 1105 60-61 0.49996934753975 1105 62-63 0.613642068906092 1105 64-65 0.5762204379469331 1105 66-67 0.6046505526919859 1105 68-69 0.43714067871114537 1105 70-71 0.3728389117444344 1105 72-73 0.37559872845023534 1105 74-75 0.2782103388931425 1105 76-77 0.1854575023117242 1105 78-79 0.21231670673334335 1105 80-81 0.10326973129627248 1105 82-83 -0.19022478855163527 1105 84-85 -0.204653199816768 1105 86-87 -0.07727520122982767 1105 88-89 -0.19006976543442988 1105 90-91 -3.0927917794018285 1105 92-93 -7.846499160402816 1105 94-95 -5.697074119037682 1105 96-97 -3.212438855178492 1105 98-99 -2.2003428485450787 1105 100-101 -1.5886402500268666 1106 1 1.633447772175188 1106 2 1.019357591815158 1106 3 0.779735530688459 1106 4 0.8075831209612048 1106 5 0.9142062597589558 1106 6 0.8648743552515228 1106 7 0.9215181710340161 1106 8 -0.09293270328018366 1106 9 -0.367498470207984 1106 10-11 -0.3827201086576082 1106 12-13 -0.08528813439136229 1106 14-15 0.8107402983062357 1106 16-17 0.9047471867601615 1106 18-19 0.9535034875138138 1106 20-21 0.8781622461704686 1106 22-23 0.8451246135539492 1106 24-25 0.8618279228866967 1106 26-27 0.9875713402577304 1106 28-29 0.8375123195437268 1106 30-31 0.9307750778084554 1106 32-33 1.0808477493687505 1106 34-35 1.0608742448542614 1106 36-37 1.0599520593913425 1106 38-39 1.0145966625066976 1106 40-41 0.8215813695136589 1106 42-43 1.0570612752789614 1106 44-45 0.9130627483433216 1106 46-47 0.9923094286916694 1106 48-49 0.9473871524514088 1106 50-51 1.041550446137034 1106 52-53 1.1248580218252755 1106 54-55 1.2257237502740672 1106 56-57 1.2296497858728621 1106 58-59 1.1372115562506515 1106 60-61 0.8484983815692644 1106 62-63 1.0258800607042922 1106 64-65 0.755605214124504 1106 66-67 0.6370207166394835 1106 68-69 0.7360309773772471 1106 70-71 0.8860345371540852 1106 72-73 0.7893895049643795 1106 74-75 0.630560773340715 1106 76-77 0.5765557556641916 1106 78-79 0.43598026008049473 1106 80-81 0.20313897630521538 1106 82-83 0.017357008050723266 1106 84-85 -0.015093006020379107 1106 86-87 0.23901303989964973 1106 88-89 -0.030735508733929606 1106 90-91 -3.2986027924928294 1106 92-93 -8.247150942479308 1106 94-95 -5.8215250255760935 1106 96-97 -3.167833522677739 1106 98-99 -2.155170431315975 1106 100-101 -1.6257102864301167 1107 1 1.6979815865498367 1107 2 1.0564189953239307 1107 3 0.8463349930540502 1107 4 0.7700795838927306 1107 5 0.7452935546089847 1107 6 0.5894534441026806 1107 7 0.45002340589535805 1107 8 0.5087261818358328 1107 9 0.669605378122526 1107 10-11 0.5183339377487215 1107 12-13 0.451131291188716 1107 14-15 0.5438384165858068 1107 16-17 0.618018267688285 1107 18-19 0.647280015527393 1107 20-21 0.5473667594698455 1107 22-23 0.4965924969721769 1107 24-25 0.4591963439393325 1107 26-27 0.6636413034722182 1107 28-29 0.3422466857010562 1107 30-31 0.5415083121038649 1107 32-33 0.7424351857015168 1107 34-35 0.6406811209153815 1107 36-37 0.5869540401496849 1107 38-39 0.5228981622237328 1107 40-41 0.6938985030733633 1107 42-43 0.7217896284198773 1107 44-45 0.5099695819596235 1107 46-47 0.3659264349169149 1107 48-49 0.7046186889539605 1107 50-51 0.688772078847844 1107 52-53 0.6810213636475666 1107 54-55 0.48102581592205595 1107 56-57 0.5582699754597336 1107 58-59 0.378340588508717 1107 60-61 0.2113397086773574 1107 62-63 0.24219302052319236 1107 64-65 0.3318697161052633 1107 66-67 0.47540675229313223 1107 68-69 0.43619014545873824 1107 70-71 0.3778603719022442 1107 72-73 0.42441001996155236 1107 74-75 0.3721779210486922 1107 76-77 0.40470905221200937 1107 78-79 0.22405503370811175 1107 80-81 0.025774446027913456 1107 82-83 -0.13386504910830155 1107 84-85 0.0788037823214438 1107 86-87 0.4368925673473001 1107 88-89 0.20626222754224344 1107 90-91 -3.0048598666297828 1107 92-93 -4.546595622728319 1107 94-95 -2.8458317601544767 1107 96-97 -1.3223503591236927 1107 98-99 -1.0413228082259938 1107 100-101 -0.8127699214046515 1108 1 1.6627198929677078 1108 2 1.0638760072894584 1108 3 -0.3983788384244882 1108 4 -0.2536093333526921 1108 5 0.4446832500319644 1108 6 0.623830797462233 1108 7 0.6216863420513334 1108 8 0.5107103254221457 1108 9 0.7645090472715452 1108 10-11 0.6938176838787626 1108 12-13 0.601055983683068 1108 14-15 0.6267130191295252 1108 16-17 0.6980806055679594 1108 18-19 0.583482268476935 1108 20-21 0.5412187383837406 1108 22-23 0.42015511725499977 1108 24-25 0.4843140119169007 1108 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1.3453076118579723 2311 86-87 1.597206949384482 2311 88-89 2.1165046012002726 2311 90-91 2.8702154148575154 2311 92-93 3.7833467705683574 2311 94-95 3.361202967522928 2311 96-97 2.400110527233995 2311 98-99 2.107224750035334 2311 100-101 1.9647421890954782 2312 1 1.2464486594043862 2312 2 0.5814983964128615 2312 3 -0.010684711379447265 2312 4 -0.4411224513678391 2312 5 -0.07797420530805255 2312 6 -0.21877348096862193 2312 7 0.1627551274406045 2312 8 0.1899129850507677 2312 9 0.2625451629293707 2312 10-11 0.265869263131151 2312 12-13 0.4714125979037789 2312 14-15 0.37285627372866514 2312 16-17 0.08453855547671907 2312 18-19 -0.3352995476045706 2312 20-21 0.3232045219795694 2312 22-23 0.6279032631116621 2312 24-25 0.5456333416059138 2312 26-27 0.535123349103543 2312 28-29 0.5517343056129036 2312 30-31 0.5737240886141066 2312 32-33 0.6851302855200245 2312 34-35 0.663739035097386 2312 36-37 0.761111416348804 2312 38-39 0.630515805469777 2312 40-41 0.7245870239445011 2312 42-43 0.8864302169601999 2312 44-45 0.6183156086643109 2312 46-47 0.7273407465580846 2312 48-49 0.6576108136622771 2312 50-51 0.48704875113200075 2312 52-53 0.4967038889262838 2312 54-55 0.7185663266817137 2312 56-57 0.658805527702409 2312 58-59 0.7397014259471391 2312 60-61 0.7000331556568753 2312 62-63 0.5281933122006635 2312 64-65 0.4631059424469832 2312 66-67 0.7113965111730884 2312 68-69 0.7987792691299873 2312 70-71 0.905012303455262 2312 72-73 0.8832826865417935 2312 74-75 0.984915314100288 2312 76-77 1.255547624936927 2312 78-79 1.1765286206928138 2312 80-81 1.3386301304977088 2312 82-83 1.343738172307308 2312 84-85 1.620227654501896 2312 86-87 1.8366049409537304 2312 88-89 2.1349851663547916 2312 90-91 3.001399855952535 2312 92-93 4.0630167703232765 2312 94-95 3.6937788504236906 2312 96-97 2.975067485055721 2312 98-99 2.878669251665114 2312 100-101 2.729374232082513 2313 1 -6.986295569803186 2313 2 -2.567841821293804 2313 3 -1.6452277990930355 2313 4 -0.7547294441697971 2313 5 -0.4918842635340681 2313 6 -0.1657774238509404 2313 7 -0.1299979362181034 2313 8 -0.14139006195220105 2313 9 -0.07458499516970107 2313 10-11 -0.12456627300720413 2313 12-13 -0.3025622680443689 2313 14-15 -0.3139550938535862 2313 16-17 -0.5441846648534181 2313 18-19 -0.5901662191579433 2313 20-21 -0.39460360348959256 2313 22-23 -1.0889877891474598 2313 24-25 -0.4495716564214973 2313 26-27 -0.46963701736883223 2313 28-29 -0.7346872255026327 2313 30-31 -0.3226378598467292 2313 32-33 -0.6169961423068813 2313 34-35 -0.44088444919172076 2313 36-37 -0.4049316514085959 2313 38-39 -0.3583333863813465 2313 40-41 -0.2806011053470385 2313 42-43 -0.5853293297733586 2313 44-45 -0.7596568080454666 2313 46-47 -1.6570390937770583 2313 48-49 -1.1818112839019932 2313 50-51 -1.0944365879773699 2313 52-53 -1.4179799033482148 2313 54-55 -1.1890736986012413 2313 56-57 -0.8896594192525065 2313 58-59 -0.7030204273840859 2313 60-61 -0.5689638885245927 2313 62-63 -0.650938088938684 2313 64-65 -0.7955002118112837 2313 66-67 -0.5405847641655157 2313 68-69 -0.6390384468228341 2313 70-71 -0.5817169271415636 2313 72-73 -0.5846866714983214 2313 74-75 -0.4188546497636594 2313 76-77 -0.13125799564680563 2313 78-79 -0.5190260519719629 2313 80-81 -0.2783697885262235 2313 82-83 0.20566475809512497 2313 84-85 0.2686035949372787 2313 86-87 0.9335806957198081 2313 88-89 1.2469368412930457 2313 90-91 1.8801570881519964 2313 92-93 2.9413148183010236 2313 94-95 2.564450228413861 2313 96-97 1.603467088925381 2313 98-99 1.258132721862081 2313 100-101 1.088571905037428 2314 1 0.41697776191232094 2314 2 -0.04126695626871779 2314 3 -1.3331738386782064 2314 4 -0.49558012530434326 2314 5 -0.34113030737786687 2314 6 -3.474489663394916 2314 7 -2.739013890436574 2314 8 -2.6894000312021866 2314 9 -3.2508860959531205 2314 10-11 -2.4738831491659568 2314 12-13 -1.2587320330655203 2314 14-15 -1.3345248077711815 2314 16-17 -2.357191312661726 2314 18-19 -3.224672150000309 2314 20-21 -3.1210905823663033 2314 22-23 -4.191112343355329 2314 24-25 -3.3144484173674087 2314 26-27 -2.098054217869091 2314 28-29 -1.977582918134999 2314 30-31 -1.9948554076619232 2314 32-33 -2.104997862754768 2314 34-35 -1.6587480886323505 2314 36-37 -2.0643236252719994 2314 38-39 -3.2312519568374825 2314 40-41 -2.472846671277921 2314 42-43 -1.749791439216672 2314 44-45 -2.177407840038981 2314 46-47 -1.824811153359633 2314 48-49 -2.4522659134142195 2314 50-51 -3.150895640477586 2314 52-53 -2.070232346821662 2314 54-55 -2.5284079501634373 2314 56-57 -1.7490075900647106 2314 58-59 -1.2038603721358463 2314 60-61 -1.522797780517429 2314 62-63 -1.1437412939778078 2314 64-65 -1.636523864782962 2314 66-67 -1.1014865044387214 2314 68-69 -1.0170463215221588 2314 70-71 -1.1581542171734824 2314 72-73 -2.0505447714366873 2314 74-75 -2.0915759423244893 2314 76-77 -1.2222434802851758 2314 78-79 -2.0610996745103733 2314 80-81 -1.0246787103173993 2314 82-83 -0.7699550198322882 2314 84-85 -1.5857395751231422 2314 86-87 -0.3709260742947649 2314 88-89 0.720222001570594 2314 90-91 1.8633345823451464 2314 92-93 2.9161718572514594 2314 94-95 2.741081445446291 2314 96-97 1.9756214718909355 2314 98-99 1.5762184860346036 2314 100-101 1.4299214735686832 2315 1 -2.524422727485824 2315 2 -1.633326035539735 2315 3 -2.6585081954464407 2315 4 -3.9423347672824995 2315 5 -3.696419572336115 2315 6 -2.9357921111854104 2315 7 -5.536916814019012 2315 8 -2.8284681904883158 2315 9 -2.263980244910883 2315 10-11 -3.5867365371562734 2315 12-13 -2.6688939843490367 2315 14-15 -1.6771259332998554 2315 16-17 -2.2523642182554227 2315 18-19 -1.759491883490817 2315 20-21 -1.0909759733831947 2315 22-23 -0.6109799015388049 2315 24-25 -1.9018624560744257 2315 26-27 -3.524511218063566 2315 28-29 -2.9424622587443423 2315 30-31 -2.804789444907083 2315 32-33 -3.6236085478564917 2315 34-35 -3.3827915466855067 2315 36-37 -2.598986441976894 2315 38-39 -1.5206547487616717 2315 40-41 -1.266322668628355 2315 42-43 -2.397274488913638 2315 44-45 -5.704805055241845 2315 46-47 -2.615326789003717 2315 48-49 -1.678234942900101 2315 50-51 -2.7754845817721794 2315 52-53 -2.985057510645319 2315 54-55 -1.4618117070752454 2315 56-57 -0.8128100057125707 2315 58-59 -2.208244677744144 2315 60-61 -2.4339271997117145 2315 62-63 -4.270600517450294 2315 64-65 -3.6046669226780708 2315 66-67 -3.132848956195666 2315 68-69 -1.5313748487811836 2315 70-71 -3.244188451151796 2315 72-73 -2.2500109101610413 2315 74-75 -1.369996280278702 2315 76-77 -2.9060718995753305 2315 78-79 -1.5090947689879428 2315 80-81 -1.1795611963490202 2315 82-83 -0.9056985679961755 2315 84-85 -2.3398417089180903 2315 86-87 -1.7701977475652662 2315 88-89 -0.9575914429988579 2315 90-91 0.014469794299735383 2315 92-93 0.5699252510831307 2315 94-95 0.529596645165423 2315 96-97 0.05202339429366276 2315 98-99 -0.2328195563647153 2315 100-101 -0.5461188011996967 2316 1 -7.167006365980562 2316 2 -6.063660084952517 2316 3 -3.449828252302744 2316 4 -4.296970479560137 2316 5 -2.1828734078852285 2316 6 -2.9424325851199384 2316 7 -3.238733539620924 2316 8 -2.7705862549256715 2316 9 -7.095595977539361 2316 10-11 -6.900183044467319 2316 12-13 -6.135472437095828 2316 14-15 -5.05288588286027 2316 16-17 -5.175205566579692 2316 18-19 -4.422810051098114 2316 20-21 -3.1269035325848265 2316 22-23 -3.904656463344061 2316 24-25 -3.677893892036728 2316 26-27 -4.6420362273538665 2316 28-29 -4.762321706197554 2316 30-31 -7.484155477747102 2316 32-33 -5.159438174384917 2316 34-35 -5.933255561271515 2316 36-37 -4.824375083000319 2316 38-39 -6.651621975486137 2316 40-41 -4.2976044401963485 2316 42-43 -4.987782321983985 2316 44-45 -3.5985554497668133 2316 46-47 -4.867457596397934 2316 48-49 -5.8901311759943376 2316 50-51 -5.172872004172515 2316 52-53 -6.784085241452669 2316 54-55 -5.700687540048516 2316 56-57 -5.000565675189375 2316 58-59 -3.8540926144890015 2316 60-61 -3.90017789734895 2316 62-63 -4.837741029227285 2316 64-65 -3.725740501092176 2316 66-67 -4.76821612760946 2316 68-69 -3.7339409190059385 2316 70-71 -3.233741855807793 2316 72-73 -2.8353406595843325 2316 74-75 -2.842969896258502 2316 76-77 -3.5110338500705787 2316 78-79 -5.315068534315074 2316 80-81 -6.189425784203092 2316 82-83 -3.75667362081861 2316 84-85 -2.4311690894351727 2316 86-87 -2.4352497492824945 2316 88-89 -1.3679534543188119 2316 90-91 -1.1566795275856947 2316 92-93 -1.2750407274255444 2316 94-95 -1.9557961643835249 2316 96-97 -3.428662579373732 2316 98-99 -4.615962831120472 2316 100-101 -3.887079014028684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24449.0 3 8649.0 4 8358.0 5 9232.0 6 10480.0 7 12275.0 8 14769.0 9 17721.0 10 20990.0 11 24558.0 12 28313.0 13 31776.0 14 34764.0 15 38068.0 16 42256.0 17 45044.0 18 42721.0 19 41809.0 20 44579.0 21 48986.0 22 54317.0 23 60299.0 24 67634.0 25 75835.0 26 86030.0 27 96931.0 28 109876.0 29 125202.0 30 141270.0 31 159713.0 32 178868.0 33 197118.0 34 224910.0 35 260273.0 36 274805.0 37 213473.0 38 61627.0 39 1019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90042893305033 19.609871933333107 13.662693045424657 22.827006088191908 2 15.771729433033254 19.80018183868272 40.595026040948106 23.83306268733591 3 19.491369234076377 24.299858518735793 29.679144476115628 26.529627771072207 4 12.809747282332795 16.445796227258512 37.30152835348174 33.44292813692695 5 13.62376220084675 37.47102660850867 34.41674399717659 14.48846719346799 6 32.09318061947783 36.29268671626596 17.45070167237481 14.163430991881398 7 28.551919040168066 32.83374748687586 20.66577894323194 17.948554529724138 8 26.39902129167689 35.17747607753333 19.613832774460892 18.809669856328888 9 26.753513468195344 16.62284452130029 20.054910880581446 36.568731129922924 10-11 25.063178419332267 26.437742483552995 27.453828337022678 21.04525076009206 12-13 26.01234423605685 23.851983661419133 27.798418141317754 22.33725396120626 14-15 22.87155274719564 25.356599139374186 25.93364951520578 25.838198598224395 16-17 22.203656745750827 28.524289964232636 26.756895223072803 22.515158066943737 18-19 22.203169230328804 26.94835823154003 29.141892854440748 21.706579683690418 20-21 22.891455804436504 26.211974630923287 29.28464445580154 21.61192510883867 22-23 22.50373711316042 26.19286316651151 29.153625055168124 22.149774665159946 24-25 22.207230679097012 26.41304340412646 29.379663851446974 22.000062065329555 26-27 21.92223122994791 26.646535138713478 29.19400105932673 22.237232572011877 28-29 21.906099445241445 26.998374756771977 29.163507039907046 21.932018758079536 30-31 22.1886857051856 26.581944808426105 29.02663413651037 22.202735349877926 32-33 21.889589619748502 26.62989833122159 29.038783568966803 22.4417284800631 34-35 22.46899760000668 26.84865720910971 28.802886334222894 21.87945885666071 36-37 22.575887546396302 26.39279272274786 28.858248162263994 22.173071568591844 38-39 22.069830750734614 26.782269299933105 28.80807437447875 22.339825574853535 40-41 22.42714872909971 26.59120617164123 28.96602777402224 22.015617325236825 42-43 22.164414873631145 26.578323596740727 28.782320157167497 22.474941372460638 44-45 22.170440197216042 26.859085785024423 28.814898494900582 22.155575522858953 46-47 22.524287205615508 26.96845241982081 28.24508966331141 22.26217071125227 48-49 22.218523019972622 27.12338094047442 28.148977183843567 22.509118855709396 50-51 22.264619759359675 27.31605737926367 28.097599464030846 22.321723397345806 52-53 22.512778151974004 27.105220472265014 28.03362887917779 22.348372496583195 54-55 22.043924076065842 27.302062109224828 28.17238061028554 22.48163320442379 56-57 22.065624109053854 27.438208408758246 27.997108730556057 22.499058751631836 58-59 22.08152728091206 27.076974494163224 28.028916163927605 22.812582060997112 60-61 21.935328580234454 27.222764688875678 28.254623770585702 22.58728296030417 62-63 21.990063455542682 27.084796766523596 28.378180511561414 22.546959266372312 64-65 21.769862659704906 27.153494497507296 28.16985461405333 22.906788228734463 66-67 21.792324124421484 27.288946833948934 27.98712874139568 22.931600300233903 68-69 21.997281344067492 26.956373652562316 28.1786607670401 22.867684236330092 70-71 21.886748771219068 26.959566965854286 27.941568826478623 23.21211543644802 72-73 21.839441909578383 27.00356548674317 27.95117868429651 23.20581391938194 74-75 21.809294380955567 27.296360544380104 27.775159093591167 23.11918598107317 76-77 21.81683406765993 26.995610414383975 27.91403603089766 23.27351948705843 78-79 21.922676373480368 27.18729927420623 27.725939426562547 23.164084925750856 80-81 22.012302899277046 27.10237075251972 27.592719516617713 23.292606831585516 82-83 21.986981713365363 27.113083465063355 27.67272070386562 23.227214117705657 84-85 21.8728858693919 27.096155968032427 27.623628090712927 23.40733007186275 86-87 21.64339884342197 26.966816214467475 27.964279069259785 23.42550587285077 88-89 21.796097769459603 26.934150051496836 27.629131365453237 23.64062081359032 90-91 21.573674837818064 26.896758890146877 27.968887177144058 23.560679094891 92-93 22.021322068155737 26.47933312411898 28.004755576834427 23.494589230890853 94-95 21.53053529064541 26.452185793748907 28.13383478103826 23.883444134567426 96-97 21.944937581526847 26.209736442445585 27.731769974099073 24.113556001928497 98-99 21.729962630289705 26.35050938372573 27.527284939859992 24.39224304612458 100-101 21.857101965637824 26.34090547934707 27.671283895479366 24.130708659535742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3701.0 1 2179.0 2 650.0 3 638.0 4 572.5 5 533.5 6 655.5 7 1146.0 8 1839.5 9 2251.5 10 2217.0 11 2019.0 12 2008.0 13 2172.5 14 2384.5 15 2620.5 16 2938.0 17 3286.0 18 3643.0 19 4172.0 20 4889.5 21 5788.5 22 6939.0 23 8532.0 24 10668.5 25 13484.0 26 17614.0 27 22446.0 28 26588.5 29 30649.0 30 35518.5 31 41995.0 32 50049.5 33 55373.0 34 59347.5 35 64789.0 36 71358.5 37 77535.5 38 81009.0 39 83759.5 40 87159.5 41 90976.0 42 93923.0 43 96363.0 44 99775.5 45 103713.0 46 105895.0 47 107375.0 48 109130.0 49 108019.0 50 105489.5 51 103694.0 52 101782.0 53 100595.5 54 98738.5 55 92697.5 56 84633.0 57 76716.5 58 69533.5 59 61778.0 60 51682.0 61 42296.5 62 34579.0 63 26596.0 64 19616.0 65 14871.0 66 11008.5 67 8118.0 68 6130.0 69 4632.5 70 3445.0 71 2531.5 72 1881.0 73 1434.5 74 1094.5 75 830.5 76 632.5 77 465.5 78 320.5 79 199.5 80 143.5 81 111.5 82 85.5 83 56.0 84 34.0 85 22.5 86 19.5 87 14.5 88 7.5 89 6.5 90 7.5 91 4.5 92 2.5 93 3.5 94 4.0 95 3.5 96 3.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12953398727525073 2 0.11663843141044376 3 0.12337542365643789 4 0.13416141629270123 5 0.12061938137398576 6 0.13640708037469926 7 0.16672354548167284 8 0.15369188876341147 9 0.16291272158494888 10-11 0.15816620432072573 12-13 0.14794162770496191 14-15 0.1532665735963664 16-17 0.14761838817800768 18-19 0.16274259551813083 20-21 0.1685098691832622 22-23 0.20134420007914267 24-25 0.22466848383989502 26-27 0.23633913202361212 28-29 0.2320689677464795 30-31 0.2224738575779424 32-33 0.22397096696594113 34-35 0.2136953525301319 36-37 0.22172530288394307 38-39 0.20969738995990808 40-41 0.21410365509049517 42-43 0.2067031711839107 44-45 0.19967696462432594 46-47 0.21543063841167584 48-49 0.2529434361450522 50-51 0.25467872202659614 52-53 0.256158818807913 54-55 0.25464469681323254 56-57 0.22868345901680062 58-59 0.2288876102969823 60-61 0.2005105823517343 62-63 0.19338230015205868 64-65 0.19515161124696623 66-67 0.20246703212014167 68-69 0.20176951524618772 70-71 0.20791106625831873 72-73 0.2381424683318833 74-75 0.24072838454751738 76-77 0.25563142800077715 78-79 0.2534197891321427 80-81 0.26600911807667715 82-83 0.2658219794031773 84-85 0.24118772492792612 86-87 0.24356948986337854 88-89 0.24714213726655726 90-91 0.43579493276107467 92-93 0.576863467366588 94-95 0.33610105760570697 96-97 0.27948310256866543 98-99 0.30908503819500327 100-101 0.3280881198585776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2938997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.165341225696935 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59023392046544 45.13062431765517 2 12.518760016612335 14.563158961777356 3 4.260053889032742 7.433624642863402 4 1.9349623656962456 4.501909850384156 5 1.050263335252648 3.05444626359044 6 0.6070516023721921 2.118561815615079 7 0.41211366025976054 1.6779512170945938 8 0.2789236545033411 1.2978951632084188 9 0.20104968459680994 1.0524711157102975 >10 1.0434337166657586 10.794292391986254 >50 0.06769784558971717 2.7009565508898428 >100 0.032172447864363636 3.2828749544716103 >500 0.0019703166533562894 0.7729181060889055 >1k 0.0011941313048537364 1.0806475873408004 >5k 1.1941313048537364E-4 0.5376670613237224 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8672 0.2950666502891973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6810 0.23171170300616165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4141 0.14089840853869534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.361008534544268E-4 2 0.0 0.0 0.0 0.0 1.361008534544268E-4 3 0.0 0.0 0.0 0.0 1.361008534544268E-4 4 0.0 0.0 0.0 0.0 1.361008534544268E-4 5 0.0 0.0 0.0 0.0 1.361008534544268E-4 6 1.361008534544268E-4 0.0 0.0 0.0 1.361008534544268E-4 7 1.701260668180335E-4 0.0 0.0 3.40252133636067E-5 1.361008534544268E-4 8 1.701260668180335E-4 0.0 0.0 3.40252133636067E-5 1.361008534544268E-4 9 1.701260668180335E-4 0.0 0.0 6.80504267272134E-5 1.361008534544268E-4 10-11 1.701260668180335E-4 0.0 0.0 6.80504267272134E-5 1.361008534544268E-4 12-13 1.701260668180335E-4 0.0 0.0 6.80504267272134E-5 2.041512801816402E-4 14-15 2.722017069088536E-4 0.0 0.0 6.80504267272134E-5 2.5518910022705025E-4 16-17 2.892143135906569E-4 0.0 0.0 1.361008534544268E-4 3.40252133636067E-4 18-19 3.2323952695426366E-4 0.0 0.0 2.3817649354524687E-4 3.40252133636067E-4 20-21 3.40252133636067E-4 0.0 0.0 3.742773469996737E-4 4.083025603632804E-4 22-23 4.083025603632804E-4 0.0 0.0 6.80504267272134E-4 4.423277737268871E-4 24-25 5.103782004541005E-4 0.0 0.0 0.0010547816142718078 4.423277737268871E-4 26-27 5.103782004541005E-4 0.0 0.0 0.0024838405755432894 4.5934038040869047E-4 28-29 6.80504267272134E-4 0.0 0.0 0.006617903999221502 4.763529870904938E-4 30-31 7.825799073629541E-4 0.0 0.0 0.023171170300616162 4.763529870904938E-4 32-33 7.825799073629541E-4 0.0 0.0 0.06003748898008403 4.763529870904938E-4 34-35 8.166051207265609E-4 0.0 0.0 0.11406952780149146 5.444034138177072E-4 36-37 9.186807608173809E-4 0.0 0.0 0.20455958274200348 5.444034138177072E-4 38-39 9.527059741809876E-4 0.0 0.0 0.3436036171523823 5.614160204995105E-4 40-41 9.527059741809876E-4 0.0 0.0 0.4968021403220214 5.784286271813139E-4 42-43 0.0010377690075900042 0.0 0.0 0.6440292385463475 5.784286271813139E-4 44-45 0.0011568572543626278 0.0 0.0 0.8269317729824155 5.954412338631172E-4 46-47 0.0012759455011352513 0.0 0.0 1.0414777558466375 6.124538405449206E-4 48-49 0.001361008534544268 0.0 0.0 1.272934269752572 6.124538405449206E-4 50-51 0.001361008534544268 0.0 0.0 1.51932785232513 6.124538405449206E-4 52-53 0.001361008534544268 0.0 0.0 1.7536084589402439 6.464790539085273E-4 54-55 0.0013950337479078747 0.0 0.0 2.00932494997443 6.634916605903307E-4 56-57 0.0013950337479078747 0.0 0.0 2.3054293692712173 7.31542087317544E-4 58-59 0.0013950337479078747 0.0 0.0 2.6235991394343037 7.825799073629541E-4 60-61 0.0013950337479078747 0.0 0.0 2.9573014194978766 7.825799073629541E-4 62-63 0.001463084174635088 0.0 0.0 3.2792479883443226 7.825799073629541E-4 64-65 0.001463084174635088 0.0 0.0 3.6059580870616745 7.825799073629541E-4 66-67 0.0015651598147259083 0.0 0.0 3.979418828940622 7.825799073629541E-4 68-69 0.0015651598147259083 0.0 0.0 4.389048372625083 7.825799073629541E-4 70-71 0.0015821724214077114 0.0 0.0 4.82326113296475 7.825799073629541E-4 72-73 0.0016161976347713182 0.0 0.0 5.253322817274056 7.825799073629541E-4 74-75 0.0016332102414531216 0.0 0.0 5.672615521553782 7.825799073629541E-4 76-77 0.0016332102414531216 0.0 0.0 6.115725875188032 7.825799073629541E-4 78-79 0.0016332102414531216 0.0 0.0 6.608938355500193 7.995925140447575E-4 80-81 0.0017182732748621385 0.0 0.0 7.10938119365212 8.336177274083642E-4 82-83 0.0017693110949075484 0.0 0.0 7.587622580084294 8.506303340901675E-4 84-85 0.0018033363082711551 0.0 0.0 8.03738486293113 8.506303340901675E-4 86-87 0.001854374128316565 0.0 0.0 8.493901150630641 9.356933674991843E-4 88-89 0.0018713867349983684 0.0 0.0 8.974218075077994 0.001020756400908201 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5330 0.0 51.887573 1 GTATCAA 9645 0.0 41.707645 1 ATCAACG 12225 0.0 33.837982 3 TATCAAC 12415 0.0 33.66345 2 TCAACGC 12510 0.0 33.25783 4 CAACGCA 12585 0.0 33.022495 5 AACGCAG 13030 0.0 31.96862 6 ACGCAGA 14860 0.0 27.712437 7 CGCAGAG 15015 0.0 27.362701 8 TACATGG 10660 0.0 26.478243 2 GTACATG 10845 0.0 25.326258 1 ACATGGG 10920 0.0 24.892517 3 GCAGAGT 15740 0.0 24.806273 9 TGGTATC 2495 0.0 23.576565 2 GTGGTAT 2680 0.0 22.656729 1 GAGTACT 9555 0.0 22.46518 12-13 CAGAGTA 15165 0.0 21.56875 10-11 CATGGGG 6080 0.0 21.145468 4 AGAGTAC 14295 0.0 20.973265 10-11 GTACTTT 10345 0.0 20.752445 14-15 >>END_MODULE