FastQCFastQC Report
Fri 27 May 2016
SRR939875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938997
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95370.3244984598487171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80610.2742772449240336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.18091205945429684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.053.6932331
GTATCAA93300.043.0846251
ATCAACG120650.032.9585843
TCAACGC122150.032.6283074
TATCAAC123100.032.496432
CAACGCA124950.031.897145
AACGCAG131100.030.4535946
ACGCAGA151850.026.1680037
CGCAGAG152800.025.8823578
TACATGG106750.023.8629262
TGGTATC24600.023.7784982
GTACATG111000.023.4628331
TAAGGTG16550.023.0843855
GCAGAGT168650.022.8349979
ACATGGG110100.022.6211593
GTGGTAT27850.022.5315381
GAGTACT102050.020.90135612-13
AGAGTAC153450.020.51987810-11
AGTACTT107700.019.69544212-13
CAGAGTA162100.019.5702910-11
Produced by FastQC (version 0.11.5)