FastQCFastQC Report
Fri 27 May 2016
SRR939874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056990
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89900.294080124566976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69620.22774035898056585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.14455395666979612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.052.2557641
GTATCAA98450.041.806721
ATCAACG127700.031.4598273
TATCAAC128500.031.3351352
TCAACGC129400.031.265164
CAACGCA131700.030.6864435
AACGCAG137150.029.5013436
ACGCAGA153000.025.9839067
CGCAGAG153200.025.9197038
TACATGG114450.025.358892
GTACATG115900.025.244151
TGGTATC26450.024.3644032
ACATGGG118600.023.9178413
GCAGAGT163150.023.2719469
GAGTACT96750.021.8571212-13
GTGGTAT29950.021.673851
AGAGTAC146500.020.857710-11
CAGAGTA156950.020.77288410-11
GTACTTT103600.020.43916314-15
AGTACTT102250.019.9222712-13
Produced by FastQC (version 0.11.5)