##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939874_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3056990 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.08769868399962 31.0 30.0 33.0 26.0 34.0 2 30.15662367230511 31.0 30.0 33.0 26.0 34.0 3 30.104240445667145 31.0 30.0 34.0 26.0 34.0 4 33.75141528104442 35.0 35.0 37.0 28.0 37.0 5 33.62105796878629 35.0 33.0 37.0 28.0 37.0 6 33.60780571738867 35.0 33.0 37.0 28.0 37.0 7 33.59441771153978 35.0 33.0 37.0 28.0 37.0 8 33.43137138165319 35.0 33.0 37.0 27.0 37.0 9 34.742646524849604 37.0 34.0 39.0 27.0 39.0 10-11 34.53350321721693 37.0 33.5 39.0 27.0 39.0 12-13 34.44014766158868 37.0 33.0 39.0 27.0 39.0 14-15 35.043873058138885 38.0 33.0 40.0 26.0 41.0 16-17 34.839673011687964 38.0 33.0 40.0 25.0 41.0 18-19 34.881406219843704 38.0 33.5 40.0 25.0 41.0 20-21 34.99341378283867 38.0 34.0 40.0 25.0 41.0 22-23 34.90841334122781 38.0 33.5 40.0 25.0 41.0 24-25 34.724702076225306 38.0 33.0 40.0 25.0 41.0 26-27 34.61216605222785 38.0 33.0 40.0 24.0 41.0 28-29 34.49414849247135 38.0 33.0 40.0 24.0 41.0 30-31 34.29775792527944 38.0 33.0 40.0 23.5 41.0 32-33 33.99698919525416 38.0 33.0 40.0 23.0 41.0 34-35 33.71511895688242 38.0 32.0 40.0 21.0 41.0 36-37 33.483111164904045 37.5 32.0 40.0 19.0 41.0 38-39 33.1925946764628 37.0 31.0 40.0 18.0 41.0 40-41 32.894531221888194 37.0 31.0 40.0 16.0 41.0 42-43 32.57445117582982 37.0 30.5 40.0 15.0 41.0 44-45 32.15784938779649 37.0 30.0 39.5 11.0 41.0 46-47 31.75914085423897 36.5 30.0 39.0 9.0 40.5 48-49 31.39879276674114 36.0 30.0 39.0 8.0 40.0 50-51 30.66149316811635 35.5 28.5 38.5 7.0 40.0 52-53 30.7815393573417 35.5 29.0 38.5 6.0 40.0 54-55 30.852502625131258 36.0 29.0 39.0 2.0 40.0 56-57 30.623116366098678 35.0 28.0 39.0 2.0 40.0 58-59 30.07898210331077 35.0 28.0 38.5 2.0 40.0 60-61 29.590946813695822 34.5 27.0 38.0 2.0 40.0 62-63 29.14837209150177 34.0 26.0 38.0 2.0 40.0 64-65 28.61319615046173 34.0 26.0 38.0 2.0 40.0 66-67 28.080637653377998 33.5 25.5 37.0 2.0 39.0 68-69 27.535128999440627 33.0 24.0 36.5 2.0 39.0 70-71 26.976915037340653 32.5 22.5 36.0 2.0 39.0 72-73 26.49954890267878 32.0 22.0 35.5 2.0 38.5 74-75 25.914457521941518 31.5 20.0 35.0 2.0 38.0 76-77 25.268674578588744 31.0 18.5 35.0 2.0 37.0 78-79 24.617457204635933 31.0 15.0 34.5 2.0 36.0 80-81 23.94555199722603 30.0 8.5 34.0 2.0 36.0 82-83 23.190599249588647 30.0 4.5 34.0 2.0 35.5 84-85 22.440214557456844 29.0 2.0 34.0 2.0 35.0 86-87 21.656618929077297 29.0 2.0 34.0 2.0 35.0 88-89 20.803121371021824 27.5 2.0 33.0 2.0 35.0 90-91 19.85658131037393 26.0 2.0 33.0 2.0 34.0 92-93 18.870110468140233 25.0 2.0 32.0 2.0 34.0 94-95 17.496170088878273 22.0 2.0 32.0 2.0 34.0 96-97 14.925453305375548 2.0 2.0 31.0 2.0 34.0 98-99 13.537970192902169 2.0 2.0 30.0 2.0 34.0 100-101 12.262416789063753 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8606995969732054 1101 2 0.8128756023688766 1101 3 0.9917435877355345 1101 4 1.1065081425045449 1101 5 1.042573887126288 1101 6 1.2123204658433764 1101 7 0.9903951144325092 1101 8 1.0028022779758885 1101 9 1.1248434428496168 1101 10-11 1.1276401550715747 1101 12-13 0.9379967134194729 1101 14-15 1.09371319737906 1101 16-17 1.1584863641174934 1101 18-19 1.0493608439767215 1101 20-21 1.2050286331814206 1101 22-23 1.0838539226750825 1101 24-25 1.1074294752125056 1101 26-27 1.2314480616644659 1101 28-29 1.3643041652310899 1101 30-31 1.4001058040923695 1101 32-33 1.1742466987878828 1101 34-35 1.1418552893208584 1101 36-37 1.3044514248931947 1101 38-39 1.2532044017458759 1101 40-41 1.3900262462364736 1101 42-43 1.3152235389153901 1101 44-45 1.183210582024806 1101 46-47 1.2038755298597756 1101 48-49 1.0532236573333584 1101 50-51 1.1692881889952709 1101 52-53 1.2185423922607939 1101 54-55 1.158671139659969 1101 56-57 0.9694189658139578 1101 58-59 0.8083152521708996 1101 60-61 1.0191012785398428 1101 62-63 0.9752110008670591 1101 64-65 1.2102914962113864 1101 66-67 0.7766452479408095 1101 68-69 0.8384658781665912 1101 70-71 0.8734548709522798 1101 72-73 0.67908886775475 1101 74-75 0.6424604879069911 1101 76-77 0.3513456500050225 1101 78-79 0.4952410575008237 1101 80-81 0.12340055849504594 1101 82-83 0.23114364629493167 1101 84-85 0.2341514851555111 1101 86-87 0.2001753458178861 1101 88-89 0.11890239071984965 1101 90-91 -0.21094514582126322 1101 92-93 -0.2035103831972549 1101 94-95 0.15545449084574514 1101 96-97 1.8594897365933587 1101 98-99 1.6642610289447504 1101 100-101 1.0446085308682154 1102 1 0.7727365170190374 1102 2 0.7979670994082078 1102 3 0.8904912186635698 1102 4 0.9777938124299084 1102 5 1.010603782940663 1102 6 0.9296268444023426 1102 7 0.8421313631074412 1102 8 0.7425300890069337 1102 9 1.0516638002716405 1102 10-11 0.8965168134219468 1102 12-13 0.7557927537231421 1102 14-15 0.9883728540911605 1102 16-17 0.9510611304225876 1102 18-19 1.0472404041056365 1102 20-21 0.8771085980602535 1102 22-23 0.6795050025435927 1102 24-25 0.7641224113626706 1102 26-27 1.029191848539007 1102 28-29 1.1665263279662526 1102 30-31 1.032677465173201 1102 32-33 1.181193406714769 1102 34-35 0.9606072033168545 1102 36-37 0.9262575946525899 1102 38-39 0.8384535156861901 1102 40-41 0.9195987960539043 1102 42-43 0.5919201156733251 1102 44-45 0.5726016368373692 1102 46-47 0.7862921438803916 1102 48-49 0.5187084470882546 1102 50-51 0.6990247803156358 1102 52-53 0.5118197958649979 1102 54-55 0.4626326562943355 1102 56-57 0.3422809118258847 1102 58-59 0.259397369291392 1102 60-61 0.5605538810390129 1102 62-63 0.37946237412572614 1102 64-65 0.19179463891250492 1102 66-67 0.03955644923429347 1102 68-69 -0.06529893857164026 1102 70-71 0.20661245573025866 1102 72-73 0.17620015256806099 1102 74-75 0.042711689842935385 1102 76-77 -0.127821507429843 1102 78-79 -0.2680155732237637 1102 80-81 -0.34669184869622427 1102 82-83 -0.4369888289971229 1102 84-85 -0.5319356110314857 1102 86-87 -0.6732397584212819 1102 88-89 -0.6731037983133525 1102 90-91 -0.5703562239765496 1102 92-93 -0.7115261448873547 1102 94-95 -0.5334489366793917 1102 96-97 0.6456602455289104 1102 98-99 0.5320278373095828 1102 100-101 0.14664555799074463 1103 1 0.6898536953338628 1103 2 0.6449359302377289 1103 3 0.8408740795388141 1103 4 0.6424215851274937 1103 5 0.8779889690935008 1103 6 0.7703847281384597 1103 7 0.7009079013177555 1103 8 0.608255392729987 1103 9 0.9930113117020767 1103 10-11 0.8712808108092815 1103 12-13 0.682779664239149 1103 14-15 0.9958889350839755 1103 16-17 1.045934232969941 1103 18-19 0.8833752702062228 1103 20-21 0.8667663380994455 1103 22-23 0.7739011357898349 1103 24-25 0.8960170161961116 1103 26-27 1.08927101248414 1103 28-29 1.050992689821257 1103 30-31 0.9398146565513912 1103 32-33 0.8815083381321429 1103 34-35 0.9598120106323336 1103 36-37 0.7460724084997494 1103 38-39 0.8306299755163664 1103 40-41 0.8986885413184353 1103 42-43 0.7060733749809671 1103 44-45 0.7552014016969437 1103 46-47 0.5742348741220731 1103 48-49 0.5212525097923724 1103 50-51 0.794715074241175 1103 52-53 0.8771732119329236 1103 54-55 0.7095314675288229 1103 56-57 0.6827055231910073 1103 58-59 0.49526213741679825 1103 60-61 0.6897570162447586 1103 62-63 0.6242064107031275 1103 64-65 0.5175767421130253 1103 66-67 0.32823344466212134 1103 68-69 0.21285014046167205 1103 70-71 0.5377119201326082 1103 72-73 0.41363837595147146 1103 74-75 0.22719884856272898 1103 76-77 0.0198059778738795 1103 78-79 -0.0871025490565529 1103 80-81 -0.0899253431442979 1103 82-83 -0.041539632393593706 1103 84-85 -0.2020058918936698 1103 86-87 -0.34777875254276935 1103 88-89 -0.20490351091949321 1103 90-91 -0.22069858844421475 1103 92-93 -0.25891890778742166 1103 94-95 -0.1798700993181903 1103 96-97 1.4368379333146688 1103 98-99 1.4505049633709817 1103 100-101 1.0534832849665765 1104 1 0.602006725636894 1104 2 0.6293034049852047 1104 3 0.6772943825691193 1104 4 0.8288738073497157 1104 5 0.9113841206086519 1104 6 0.9877379604616934 1104 7 0.7458647700046299 1104 8 0.7658360998006941 1104 9 1.031383432914204 1104 10-11 0.9687975279809962 1104 12-13 0.8899062804007656 1104 14-15 0.9482704502354906 1104 16-17 1.071705647111358 1104 18-19 1.1195642096001635 1104 20-21 1.0411830047661184 1104 22-23 1.0277529539716568 1104 24-25 1.0784065616506595 1104 26-27 1.2614510629891882 1104 28-29 1.261313396891964 1104 30-31 1.084350919177652 1104 32-33 1.3317581866169874 1104 34-35 1.25923130356162 1104 36-37 1.16578660041894 1104 38-39 0.9693204805668714 1104 40-41 1.0409110160659125 1104 42-43 1.0084553446779339 1104 44-45 1.124425290585826 1104 46-47 1.0627623488695441 1104 48-49 1.0192932673681305 1104 50-51 1.115345276261376 1104 52-53 1.1383871513268637 1104 54-55 1.0743161139934685 1104 56-57 1.0292881494536346 1104 58-59 0.6676425414572051 1104 60-61 1.0397633293760755 1104 62-63 0.7958783298950429 1104 64-65 0.9592600754463625 1104 66-67 0.8195751618338356 1104 68-69 0.4975386758152105 1104 70-71 0.7072397484154394 1104 72-73 0.8037274668605647 1104 74-75 0.7804856162394955 1104 76-77 0.6201598162577184 1104 78-79 0.56871452165052 1104 80-81 0.6212985962496411 1104 82-83 0.6775095595255962 1104 84-85 0.5006307242679462 1104 86-87 0.4806927121036928 1104 88-89 0.6768383072623259 1104 90-91 0.48794550246487844 1104 92-93 0.642075082111571 1104 94-95 0.9397076784595875 1104 96-97 2.174540862607598 1104 98-99 2.212531023977041 1104 100-101 2.052016214259508 1105 1 0.7239845010210679 1105 2 0.735894792796973 1105 3 0.7007747430459226 1105 4 0.7020801001353902 1105 5 0.6824993008470557 1105 6 0.8694846333517319 1105 7 0.7768323246984821 1105 8 0.793174950391915 1105 9 0.9581570936925985 1105 10-11 1.0411350920099096 1105 12-13 0.7728313546025021 1105 14-15 0.8420940535673864 1105 16-17 0.9670132851026452 1105 18-19 1.0726317630972204 1105 20-21 1.014178660374327 1105 22-23 0.9621086555370724 1105 24-25 0.9733005549006961 1105 26-27 1.10601414044622 1105 28-29 1.1043512996158782 1105 30-31 1.1091752884629216 1105 32-33 1.099974562460737 1105 34-35 1.0519161496528682 1105 36-37 1.079164604392325 1105 38-39 0.9732352203820867 1105 40-41 1.0166424117765729 1105 42-43 0.9890693939383155 1105 44-45 0.8955926813177868 1105 46-47 0.6649303559546134 1105 48-49 0.9248839789866814 1105 50-51 0.7864984549678731 1105 52-53 0.8810824694368762 1105 54-55 0.9814849588400385 1105 56-57 0.790229314660202 1105 58-59 0.7373580615874182 1105 60-61 0.9836274743806221 1105 62-63 0.9776342780017053 1105 64-65 0.6850598384795354 1105 66-67 0.5971803009022452 1105 68-69 0.4800218308250237 1105 70-71 0.7080199138134944 1105 72-73 0.8408002084949118 1105 74-75 0.5526883904268658 1105 76-77 0.3342542559149493 1105 78-79 0.007101052839182387 1105 80-81 -6.28913444455037E-4 1105 82-83 -0.136694387527875 1105 84-85 -0.2902889882601798 1105 86-87 0.06736437541931295 1105 88-89 0.16225048592094637 1105 90-91 0.14133439733777564 1105 92-93 0.28454674782079437 1105 94-95 0.43503416608465173 1105 96-97 1.816170129207249 1105 98-99 1.927774094492623 1105 100-101 1.4333472976048078 1106 1 0.923128036341538 1106 2 1.0038844253995585 1106 3 1.0036121776369136 1106 4 0.9535922758181883 1106 5 0.9901409076987733 1106 6 1.1507371338775343 1106 7 0.9526387788619672 1106 8 1.0748335442148047 1106 9 1.163716523580625 1106 10-11 1.1775256690008078 1106 12-13 1.1540364143225617 1106 14-15 1.4420132460616202 1106 16-17 1.470929681752068 1106 18-19 1.4277563980006889 1106 20-21 1.3404093144090936 1106 22-23 0.9239626105979752 1106 24-25 1.0922039764897349 1106 26-27 1.5488724806189325 1106 28-29 1.36133102815959 1106 30-31 1.2444911730679067 1106 32-33 1.44055874854255 1106 34-35 1.339081039661366 1106 36-37 1.3898302463375884 1106 38-39 1.4241882271012898 1106 40-41 1.4901343871642823 1106 42-43 1.3802196279005514 1106 44-45 1.5578430833786214 1106 46-47 1.5057451043523038 1106 48-49 1.0900614787613385 1106 50-51 1.328187853687286 1106 52-53 1.3791108295505445 1106 54-55 1.3193262756702957 1106 56-57 1.334290121122276 1106 58-59 1.0370213900027174 1106 60-61 1.379373299194377 1106 62-63 1.3519950526784363 1106 64-65 1.4236932586295445 1106 66-67 1.2062468714222128 1106 68-69 1.109608605593472 1106 70-71 1.3621432314439197 1106 72-73 1.275902293202055 1106 74-75 1.2483673237045352 1106 76-77 1.2788876729022363 1106 78-79 1.1808673942200585 1106 80-81 1.1575573929251064 1106 82-83 1.3877320926647982 1106 84-85 1.3607005478794356 1106 86-87 1.2859984913678062 1106 88-89 1.282038204128888 1106 90-91 1.2255576411186802 1106 92-93 1.4050383294498197 1106 94-95 1.861087391505965 1106 96-97 3.143218820868894 1106 98-99 3.458085290364643 1106 100-101 2.7917673290106197 1107 1 0.49577115565132246 1107 2 0.4401511683329673 1107 3 0.53218074620548 1107 4 0.11750475973067154 1107 5 0.5445515087760455 1107 6 0.6421409186146505 1107 7 0.5017612346510916 1107 8 0.5141423768569737 1107 9 0.5807268672576313 1107 10-11 0.675842080650547 1107 12-13 0.46331807693756133 1107 14-15 0.5688133795284145 1107 16-17 0.5227824869381976 1107 18-19 0.5007555876665393 1107 20-21 0.4790203063534122 1107 22-23 0.45471002467872523 1107 24-25 0.454767492386587 1107 26-27 0.6708964093095346 1107 28-29 0.6347387215672811 1107 30-31 0.6892444978212282 1107 32-33 0.7755451635289674 1107 34-35 0.7375123280926488 1107 36-37 0.45643685294419356 1107 38-39 0.5303810866274787 1107 40-41 0.6233361603660583 1107 42-43 0.5328555972031879 1107 44-45 0.42552648106202184 1107 46-47 0.3756736042808022 1107 48-49 0.40315600185586575 1107 50-51 0.4833449155110152 1107 52-53 0.5232392436789581 1107 54-55 0.5034070230843781 1107 56-57 0.32842234858783215 1107 58-59 0.1718957882104526 1107 60-61 0.44547130195904927 1107 62-63 0.26938482340154124 1107 64-65 0.36606563100191636 1107 66-67 0.45535979386847103 1107 68-69 0.3573339499854846 1107 70-71 0.49432398362467467 1107 72-73 0.15754059817369281 1107 74-75 0.3317969438008248 1107 76-77 0.2714783588262577 1107 78-79 0.2612415779275743 1107 80-81 0.2300594187604652 1107 82-83 0.2890229072889454 1107 84-85 0.26918140969363336 1107 86-87 0.2420879141238963 1107 88-89 0.2903231557471706 1107 90-91 0.19488363377800866 1107 92-93 0.005737600149089417 1107 94-95 0.2153425990945088 1107 96-97 1.7309026952194326 1107 98-99 2.059028137974156 1107 100-101 1.6096280468713378 1108 1 0.5663280991494446 1108 2 0.4980177744348673 1108 3 0.5815077202383385 1108 4 0.565922252854044 1108 5 0.7228657894432615 1108 6 0.5967203720176357 1108 7 0.5699541334322262 1108 8 0.6097292242085288 1108 9 0.7764272735462754 1108 10-11 0.5731232273434657 1108 12-13 0.42006322544741437 1108 14-15 0.48599055670559466 1108 16-17 0.5600383983117609 1108 18-19 0.6760615659136988 1108 20-21 0.5151860519309253 1108 22-23 0.5205906111475471 1108 24-25 0.6570825805840244 1108 26-27 0.7706687867289688 1108 28-29 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2.1071407089209053 2311 92-93 2.1444322089001595 2311 94-95 2.553084229414555 2311 96-97 4.0875863900273455 2311 98-99 4.224462604525311 2311 100-101 3.833200072921409 2312 1 -0.18439174516072754 2312 2 -0.3987477791903977 2312 3 -0.8400809745570115 2312 4 -0.39552190791732755 2312 5 -1.8256410772743479 2312 6 -0.5493862764983248 2312 7 -0.029902856023817037 2312 8 0.13159189968516927 2312 9 0.05333205358770243 2312 10-11 0.04349694682164085 2312 12-13 -0.004959933906143021 2312 14-15 0.35957759041627213 2312 16-17 -0.5090219339543154 2312 18-19 -0.08695202500243226 2312 20-21 0.189653509660495 2312 22-23 0.26203442790730236 2312 24-25 0.31913343041558306 2312 26-27 0.5652302087747074 2312 28-29 0.4926990267609739 2312 30-31 0.3196284432592691 2312 32-33 -0.43322105908578834 2312 34-35 0.1456006968765351 2312 36-37 0.5138162106635846 2312 38-39 0.38131016098777337 2312 40-41 0.6660814315811905 2312 42-43 0.5921635140845183 2312 44-45 0.62531132441719 2312 46-47 0.21940977365839132 2312 48-49 0.3652965283395133 2312 50-51 0.34869022107270453 2312 52-53 0.11991670497775075 2312 54-55 0.13658407958446261 2312 56-57 0.36593124189270654 2312 58-59 0.6619213027954736 2312 60-61 0.5806070935245131 2312 62-63 0.5533479253862801 2312 64-65 0.5656002351578486 2312 66-67 0.5812384523900924 2312 68-69 0.47348247431020596 2312 70-71 0.6820529402253435 2312 72-73 0.6913152847613624 2312 74-75 0.7138231453169794 2312 76-77 0.6296604446435836 2312 78-79 0.6637628605261341 2312 80-81 0.6714622302714695 2312 82-83 0.8149663336033157 2312 84-85 0.7205152827585692 2312 86-87 0.8508462860971058 2312 88-89 0.7506580346755598 2312 90-91 1.067298196718074 2312 92-93 1.240289500249677 2312 94-95 1.8523858512227669 2312 96-97 3.27462389931158 2312 98-99 3.4608700947465607 2312 100-101 2.9568422957857425 2313 1 -0.15331421297559444 2313 2 -0.5262980971420852 2313 3 -0.7754792346923232 2313 4 -0.24330662881710907 2313 5 -0.42156210381321557 2313 6 0.06273126745715274 2313 7 -0.10510282138201887 2313 8 0.17995490244027224 2313 9 0.3310165839083865 2313 10-11 -0.6064856584680243 2313 12-13 -0.1067123956144016 2313 14-15 0.006393767939748329 2313 16-17 -0.5889635448078323 2313 18-19 -0.2377649017135397 2313 20-21 -2.51667078383624 2313 22-23 -0.5433638371487177 2313 24-25 -0.022021519302136028 2313 26-27 0.1504606972978948 2313 28-29 -0.011709634279355896 2313 30-31 0.2715913391365561 2313 32-33 0.3424692550375923 2313 34-35 0.19718986481889544 2313 36-37 -0.6987239238784682 2313 38-39 -0.21453454533686767 2313 40-41 0.1309170035915983 2313 42-43 0.13191640586284592 2313 44-45 0.2566134870200827 2313 46-47 0.11951360064228567 2313 48-49 0.009663028735385382 2313 50-51 0.23578408411064444 2313 52-53 0.05407519219398793 2313 54-55 0.1520635019127745 2313 56-57 0.4101888469732451 2313 58-59 0.2492555917307122 2313 60-61 0.017149566388027893 2313 62-63 0.1089372862204776 2313 64-65 -0.09775799204807001 2313 66-67 0.26929893972738483 2313 68-69 0.400446243613537 2313 70-71 0.33959346104314747 2313 72-73 0.17940942975599228 2313 74-75 0.2948746715490387 2313 76-77 0.3812168406564318 2313 78-79 0.06089546431498505 2313 80-81 0.38598974122373875 2313 82-83 0.532301208103064 2313 84-85 0.43111527970773267 2313 86-87 0.4457810054960696 2313 88-89 0.8826536237509934 2313 90-91 0.9259533567961533 2313 92-93 0.8039042440750457 2313 94-95 1.1394450232542361 2313 96-97 2.624336997307031 2313 98-99 2.6798022317568453 2313 100-101 2.532664386526589 2314 1 -0.19195018137411424 2314 2 -0.3349380322662405 2314 3 -1.2260397812810133 2314 4 -0.24520137006793163 2314 5 -0.5789060547387166 2314 6 -0.45519259629750763 2314 7 -0.3920169668106155 2314 8 -1.9476395167075715 2314 9 -1.6506405726322058 2314 10-11 -0.9488577846784878 2314 12-13 -0.608598638906706 2314 14-15 -0.8823104421006391 2314 16-17 -0.8107091226067951 2314 18-19 -1.8986399061522121 2314 20-21 -1.4733645802354758 2314 22-23 -2.0869116255701883 2314 24-25 -1.7134979758598163 2314 26-27 -1.8399804651135767 2314 28-29 -2.9088471576525343 2314 30-31 -1.651116541414929 2314 32-33 -1.3478306895132377 2314 34-35 -1.6623026128185714 2314 36-37 -3.3050142645221747 2314 38-39 -3.7360398433587534 2314 40-41 -4.327430556234788 2314 42-43 -3.066376475670449 2314 44-45 -3.507537359027559 2314 46-47 -3.483620810657083 2314 48-49 -1.6062668801027655 2314 50-51 -1.2022909886758875 2314 52-53 -2.6110838455821685 2314 54-55 -2.0809664543014925 2314 56-57 -2.1900790208191587 2314 58-59 -4.086076127597497 2314 60-61 -2.5424387257317242 2314 62-63 -2.377365066894587 2314 64-65 -1.7374053996092478 2314 66-67 -2.802496062805311 2314 68-69 -2.7029969774283806 2314 70-71 -2.3752881942620547 2314 72-73 -1.8038840390469417 2314 74-75 -2.1779978721236404 2314 76-77 -1.2968207015033073 2314 78-79 -0.9807123615764155 2314 80-81 -0.885312136916145 2314 82-83 -0.7507358955013146 2314 84-85 -0.6461205407656081 2314 86-87 -0.4237525688757877 2314 88-89 -0.33227932280383143 2314 90-91 0.15439957488593947 2314 92-93 0.5370416387648511 2314 94-95 1.015639783109517 2314 96-97 2.595194310817055 2314 98-99 2.8055434889508994 2314 100-101 2.552934597327555 2315 1 -2.410195655315487 2315 2 -1.8728791346973352 2315 3 -1.531174232149496 2315 4 -1.663397116171204 2315 5 -1.9031180438935102 2315 6 -3.1730755316018 2315 7 -2.1483902805004256 2315 8 -1.4596599319453354 2315 9 -1.5596338831031176 2315 10-11 -3.501499189190721 2315 12-13 -2.0297050110855253 2315 14-15 -1.2905011947861524 2315 16-17 -1.9173545981988838 2315 18-19 -1.197061872650913 2315 20-21 -0.9406622333291281 2315 22-23 -1.1151853577166548 2315 24-25 -3.0238255812064843 2315 26-27 -4.304435481022356 2315 28-29 -5.755578738750177 2315 30-31 -8.461960408383675 2315 32-33 -6.967416596932793 2315 34-35 -6.273389547809224 2315 36-37 -3.985345253837913 2315 38-39 -6.376801453055062 2315 40-41 -4.282984185954287 2315 42-43 -4.042613378265788 2315 44-45 -3.1407217151861744 2315 46-47 -2.345102008994811 2315 48-49 -2.8811802174803525 2315 50-51 -2.8932496010835003 2315 52-53 -2.0157795153398013 2315 54-55 -3.45055762338027 2315 56-57 -3.3768446066791213 2315 58-59 -2.211884875570213 2315 60-61 -2.5158598668721233 2315 62-63 -3.0515603425436275 2315 64-65 -2.361816764380478 2315 66-67 -2.6517361657274883 2315 68-69 -1.9747872621357274 2315 70-71 -2.4009537941531036 2315 72-73 -1.5836101954770996 2315 74-75 -2.0379216709177896 2315 76-77 -1.3689545416066409 2315 78-79 -1.407755536069537 2315 80-81 -1.1988583301572824 2315 82-83 -0.9842959960299567 2315 84-85 -0.8224708269586039 2315 86-87 -1.3630527785167992 2315 88-89 -1.8626097446857273 2315 90-91 -1.6845788481844757 2315 92-93 -1.128789816878328 2315 94-95 -0.43612204737013727 2315 96-97 1.2617252060419428 2315 98-99 1.2522496386956554 2315 100-101 0.97256094730424 2316 1 -3.626432848201759 2316 2 -2.3264796898150415 2316 3 -2.492572991701291 2316 4 -3.0679031642128756 2316 5 -2.651080898980375 2316 6 -5.973229730964441 2316 7 -3.630820119790421 2316 8 -4.805433156082678 2316 9 -6.5825727516224575 2316 10-11 -5.59348467731737 2316 12-13 -3.8299410217766834 2316 14-15 -5.101099402646895 2316 16-17 -4.781667297373062 2316 18-19 -2.876269373593246 2316 20-21 -3.5159025273359106 2316 22-23 -4.305353006690375 2316 24-25 -3.1898794481838344 2316 26-27 -4.0877429716847296 2316 28-29 -3.903293589862127 2316 30-31 -3.596398694372091 2316 32-33 -3.460287018068385 2316 34-35 -5.9819994405550005 2316 36-37 -6.0649271165662135 2316 38-39 -5.837346119470439 2316 40-41 -5.212406709341195 2316 42-43 -4.532440503647003 2316 44-45 -3.9383174901236373 2316 46-47 -3.992130244611438 2316 48-49 -4.3868230574899485 2316 50-51 -5.265352366392069 2316 52-53 -3.329700719465489 2316 54-55 -3.6922013029197274 2316 56-57 -2.9937422868353742 2316 58-59 -3.5465891554591913 2316 60-61 -3.393461981116719 2316 62-63 -2.7125286288747112 2316 64-65 -3.066932756567713 2316 66-67 -3.7151262387151895 2316 68-69 -3.1335025244459764 2316 70-71 -2.8032248080995785 2316 72-73 -2.7362251596685816 2316 74-75 -3.8236852147618094 2316 76-77 -4.252112544553562 2316 78-79 -3.990696374914073 2316 80-81 -4.135954841825036 2316 82-83 -3.3103364133962323 2316 84-85 -3.0204204037670195 2316 86-87 -2.815541813228787 2316 88-89 -2.933394962106828 2316 90-91 -2.496804287652658 2316 92-93 -2.133979910425939 2316 94-95 -2.186519967392069 2316 96-97 -0.44926064188321924 2316 98-99 -0.05239561710395435 2316 100-101 -0.2288598284898864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25215.0 3 8458.0 4 8223.0 5 9086.0 6 10350.0 7 12647.0 8 15478.0 9 18589.0 10 22202.0 11 26052.0 12 30370.0 13 34098.0 14 37096.0 15 40263.0 16 44242.0 17 47229.0 18 44478.0 19 44787.0 20 48146.0 21 52254.0 22 57941.0 23 64937.0 24 72539.0 25 81093.0 26 91159.0 27 102239.0 28 114787.0 29 130422.0 30 147591.0 31 167345.0 32 191263.0 33 219084.0 34 251176.0 35 276020.0 36 270013.0 37 194344.0 38 45367.0 39 407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.999136691307214 18.837258123731523 13.898451186214308 23.265153998746957 2 15.776579401796647 19.823210614960747 40.57320316468194 23.82700681856066 3 19.447199313502644 24.313535395882326 29.6372306906242 26.60203459999083 4 12.864510515854038 16.417304676086015 37.20699938808749 33.511185419972456 5 13.674366499829668 37.42934540499463 34.404955320877335 14.491332774298368 6 32.13942127376897 36.29170123788151 17.43899279270013 14.129884695649384 7 28.579271884129376 32.83826555715926 20.629381854299517 17.953080704411846 8 26.434005072274342 35.154883882130875 19.57998135405222 18.83112969154256 9 26.694194380297198 16.672503529145054 20.011603835490245 36.6216982550675 10-11 25.113907015971144 26.393652360881276 27.467392954758722 21.02504766838886 12-13 26.040004822147285 23.8519063533756 27.752227458590795 22.355861365886323 14-15 22.929139228055213 25.36530632733602 25.882838295989963 25.822716148618802 16-17 22.30831744126705 28.507959353870405 26.708874815502448 22.4748483893601 18-19 22.216159449573773 26.906714241321904 29.14273068190139 21.734395627202932 20-21 22.863513223660377 26.190406355366402 29.276131933300903 21.66994848767232 22-23 22.505713538854113 26.17884913061613 29.13851552058554 22.17692180994422 24-25 22.22458630081658 26.411360384803512 29.315860424799787 22.04819288958012 26-27 22.020091777498088 26.63519444443534 29.158616656006174 22.1860971220604 28-29 21.982410910749856 26.97557595505371 29.124812569433526 21.917200564762908 30-31 22.192673657016073 26.601954161383468 28.97743805033019 22.22793413127027 32-33 21.817925195460226 26.66934198089314 29.02560203586167 22.487130787784963 34-35 22.478182051187087 26.799196209296294 28.81983308658711 21.902788652929505 36-37 22.605756870417572 26.398461688455864 28.835541747674633 22.160239693451935 38-39 22.10548965893377 26.809731657681063 28.76396554431003 22.320813139075142 40-41 22.5272032346471 26.586931140852165 28.905406235790043 21.98045938871069 42-43 22.180354595041727 26.60870417317827 28.764256090920426 22.446685140859575 44-45 22.260686910691383 26.79936902251913 28.80253898626054 22.137405080528943 46-47 22.517996849332288 26.998970156840045 28.232372479666623 22.25066051416104 48-49 22.253920394708366 27.120068932322784 28.12738118813829 22.498629484830563 50-51 22.249424031609845 27.278151536213578 28.1285237123862 22.343900719790373 52-53 22.505465620341962 27.086123679227164 28.047033116468757 22.361377583962113 54-55 22.123522587045912 27.2182778991731 28.170709609179944 22.487489904601045 56-57 22.076289094480533 27.471474131879496 27.94562929043778 22.50660748320219 58-59 22.150884982662298 27.037571498661784 28.004787969881427 22.80675554879449 60-61 21.997136524456558 27.174156739885895 28.18858294016267 22.640123795494876 62-63 21.966522808434323 27.086441449000247 28.361484449152318 22.58555129341311 64-65 21.773139826210635 27.14278223566598 28.178030048551566 22.906047889571816 66-67 21.79313264791659 27.26247904531684 27.97340215574145 22.97098615102512 68-69 22.062831176709345 26.93605799821095 28.07773841978373 22.923372405295975 70-71 21.85925110947897 26.920903760380728 27.973126700351695 23.246718429788604 72-73 21.879979357999694 27.003283835372834 27.88797846097757 23.2287583456499 74-75 21.79119702832856 27.273909194956996 27.741310997377965 23.193582779336477 76-77 21.754609146175284 26.995935667250098 27.920700089760984 23.328755096813634 78-79 21.924900641404005 27.125421372263542 27.716998517818965 23.23267946851349 80-81 21.997559197174926 27.05757661367876 27.567151181713555 23.377713007432757 82-83 21.930927421258914 27.070495112622183 27.690484435419975 23.308093030698924 84-85 21.86727188046066 27.111979033040466 27.58879145714762 23.431957629351253 86-87 21.85968256278766 26.917479635215706 27.678550645670292 23.544287156326334 88-89 21.88661102173549 26.911604686029282 27.434804517645397 23.766979774589835 90-91 21.80106914352129 26.958383399915743 27.585558529952248 23.654988926610724 92-93 21.973280312772346 26.640962973698034 27.65059087454416 23.735165838985456 94-95 21.77928166836327 26.487255631214605 27.70582671619472 24.027635984227402 96-97 21.98331299673515 26.163630993262398 27.659141647393497 24.193914362608954 98-99 21.77686961805453 26.267224030011267 27.434084599277803 24.5218217526564 100-101 21.827178229013917 26.24066513124024 27.657002492798384 24.27515414694746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3833.0 1 2234.5 2 601.0 3 535.5 4 481.0 5 513.0 6 700.0 7 1211.5 8 1947.0 9 2327.5 10 2140.0 11 1924.5 12 2048.5 13 2206.0 14 2351.0 15 2638.0 16 2956.5 17 3260.5 18 3670.0 19 4320.5 20 5069.5 21 6003.5 22 7277.0 23 8945.5 24 11128.5 25 14031.5 26 18221.0 27 23300.5 28 27814.0 29 31847.5 30 36803.0 31 43830.5 32 52492.5 33 57890.0 34 61809.5 35 67678.0 36 74569.5 37 81003.0 38 84597.0 39 87204.0 40 90855.0 41 94974.0 42 97536.5 43 100205.0 44 103651.0 45 107603.0 46 110590.5 47 111890.5 48 113197.5 49 112181.5 50 109696.5 51 107678.5 52 105751.5 53 104504.0 54 102512.5 55 96450.5 56 87599.0 57 79388.0 58 72541.0 59 64250.5 60 53686.5 61 44084.0 62 36013.0 63 27711.5 64 20376.5 65 15354.0 66 11495.5 67 8549.0 68 6378.5 69 4836.5 70 3644.0 71 2682.0 72 1945.5 73 1489.5 74 1150.5 75 866.0 76 653.0 77 455.5 78 324.5 79 223.0 80 152.0 81 108.0 82 79.0 83 51.0 84 29.0 85 20.5 86 14.5 87 13.5 88 12.0 89 8.0 90 7.5 91 4.0 92 4.0 93 3.5 94 4.0 95 3.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11844984772603115 2 0.11164576920434806 3 0.1246323998442913 4 0.13954903352644268 5 0.13444597463518035 6 0.13454411038308925 7 0.14210056297207382 8 0.1411846293249242 9 0.1488719295777873 10-11 0.14362166706466165 12-13 0.14419412559413017 14-15 0.13210707264335178 16-17 0.13424970313936258 18-19 0.15258473204034034 20-21 0.18267969473240017 22-23 0.20027870552406127 24-25 0.2193661084923405 26-27 0.22201577368588057 28-29 0.2244200995096484 30-31 0.22350416586249872 32-33 0.22509069378702581 34-35 0.19453776427139113 36-37 0.1829413900601572 38-39 0.19623878390181193 40-41 0.18490410501833504 42-43 0.18667054848069506 44-45 0.19139742033830665 46-47 0.21346160765982222 48-49 0.23035731225813627 50-51 0.24731844068838957 52-53 0.2669128783541981 54-55 0.2601415117484846 56-57 0.23025917651022737 58-59 0.22392942077010392 60-61 0.20124370704516534 62-63 0.19097216543070145 64-65 0.1830231698500813 66-67 0.17875426481604456 68-69 0.1849695288502743 70-71 0.18917301005237178 72-73 0.22564679635850954 74-75 0.2374394420655612 76-77 0.254531418159693 78-79 0.2430495356543528 80-81 0.24807081475569107 82-83 0.24540479360416617 84-85 0.23056993971193887 86-87 0.22077272087903463 88-89 0.22299713116496947 90-91 0.2252215414509043 92-93 0.23238545104825337 94-95 0.23783198505719677 96-97 8.743044628866956 98-99 0.29663165401260716 100-101 0.3078518411901903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3056990.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.11771556613591 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14687281258715 44.06453138126216 2 12.521168555467193 14.303610882136397 3 4.467329514255919 7.654909696064209 4 2.00620675018928 4.583597860966927 5 1.0726842225893694 3.0634636159070605 6 0.666240997711551 2.2832498283472185 7 0.4254381917196147 1.7010040337928503 8 0.28623035538015223 1.3079059219998028 9 0.20725122691949371 1.0653944966928297 >10 1.092737061102858 11.15860015196866 >50 0.06961949649477134 2.738125172412324 >100 0.034792316530973366 3.516532853273571 >500 0.0020919655234794693 0.8292487446128088 >1k 0.0012203132214794721 1.2003594636063533 >5k 1.1622030680756877E-4 0.5294658969569624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8990 0.294080124566976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6962 0.22774035898056585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4419 0.14455395666979612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2711915969630255E-5 2 3.2711915969630255E-5 0.0 0.0 3.2711915969630255E-5 3.2711915969630255E-5 3 3.2711915969630255E-5 0.0 0.0 3.2711915969630255E-5 3.2711915969630255E-5 4 3.2711915969630255E-5 0.0 0.0 9.813574790889077E-5 3.2711915969630255E-5 5 6.542383193926051E-5 0.0 0.0 9.813574790889077E-5 3.2711915969630255E-5 6 6.542383193926051E-5 0.0 0.0 9.813574790889077E-5 3.2711915969630255E-5 7 6.542383193926051E-5 0.0 0.0 9.813574790889077E-5 3.2711915969630255E-5 8 6.542383193926051E-5 0.0 0.0 9.813574790889077E-5 3.2711915969630255E-5 9 6.542383193926051E-5 0.0 0.0 1.3084766387852102E-4 3.2711915969630255E-5 10-11 6.542383193926051E-5 0.0 0.0 2.1262745380259667E-4 3.2711915969630255E-5 12-13 8.177978992407564E-5 0.0 0.0 2.289834117874118E-4 1.3084766387852102E-4 14-15 1.3084766387852102E-4 0.0 0.0 2.289834117874118E-4 1.7991553783296642E-4 16-17 1.4720362186333615E-4 0.0 0.0 3.271191596963026E-4 1.9627149581778153E-4 18-19 2.1262745380259667E-4 0.0 0.0 4.088989496203782E-4 1.9627149581778153E-4 20-21 2.6169532775704204E-4 0.0 0.0 5.07034697529269E-4 2.6169532775704204E-4 22-23 2.944072437266723E-4 0.0 0.0 7.850859832711262E-4 2.6169532775704204E-4 24-25 3.271191596963026E-4 0.0 0.0 0.0012430528068459498 2.6169532775704204E-4 26-27 3.271191596963026E-4 0.0 0.0 0.0021099185800411515 2.6169532775704204E-4 28-29 4.4161086559000846E-4 0.0 0.0 0.006395179572062716 2.6169532775704204E-4 30-31 5.233906555140841E-4 0.0 0.0 0.021295457296229295 2.780512857418572E-4 32-33 5.233906555140841E-4 0.0 0.0 0.056002800140007 3.271191596963026E-4 34-35 5.561025714837144E-4 0.0 0.0 0.10837457760738504 3.271191596963026E-4 36-37 6.215264034229749E-4 0.0 0.0 0.2000006542383194 3.271191596963026E-4 38-39 6.869502353622354E-4 0.0 0.0 0.34053104524385097 3.5983107566593285E-4 40-41 6.869502353622354E-4 0.0 0.0 0.49100585870415014 3.5983107566593285E-4 42-43 7.523740673014959E-4 0.0 0.0 0.6405320266013301 3.5983107566593285E-4 44-45 8.832217311800169E-4 0.0 0.0 0.8237351119892443 3.7618703365074793E-4 46-47 9.813574790889076E-4 0.0 0.0 1.042381558330253 3.9254299163556306E-4 48-49 0.0010467813110281682 0.0 0.0 1.2752086202440962 3.9254299163556306E-4 50-51 0.0010467813110281682 0.0 0.0 1.520122735108718 3.9254299163556306E-4 52-53 0.0010631372690129834 0.0 0.0 1.7562864124514639 4.906787395444538E-4 54-55 0.0010794932269977985 0.0 0.0 2.012388002577699 4.906787395444538E-4 56-57 0.001144917058937059 0.0 0.0 2.3034749868334536 4.906787395444538E-4 58-59 0.001144917058937059 0.0 0.0 2.613289542981822 4.906787395444538E-4 60-61 0.001161273016921874 0.0 0.0 2.945953372434977 4.906787395444538E-4 62-63 0.0011776289749066892 0.0 1.6355957984815128E-5 3.269212526046863 5.561025714837144E-4 64-65 0.0011776289749066892 0.0 3.2711915969630255E-5 3.5935184609697775 5.561025714837144E-4 66-67 0.0012103408908763196 0.0 3.2711915969630255E-5 3.9680862547800286 5.561025714837144E-4 68-69 0.00127576472281558 0.0 3.2711915969630255E-5 4.381352245182352 5.888144874533446E-4 70-71 0.0013084766387852103 0.0 3.2711915969630255E-5 4.812119110628428 5.888144874533446E-4 72-73 0.0013411885547548407 0.0 3.2711915969630255E-5 5.2389605461581485 5.888144874533446E-4 74-75 0.0013902564287092858 0.0 3.2711915969630255E-5 5.652030265064655 6.051704454381598E-4 76-77 0.001406612386694101 0.0 3.2711915969630255E-5 6.095145878789267 6.215264034229749E-4 78-79 0.001406612386694101 0.0 3.2711915969630255E-5 6.587525637964141 6.215264034229749E-4 80-81 0.0015538160085574373 0.0 3.2711915969630255E-5 7.106467472906356 6.215264034229749E-4 82-83 0.0015701719665422522 0.0 3.2711915969630255E-5 7.633718134504856 6.542383193926052E-4 84-85 0.0015865279245270674 0.0 3.2711915969630255E-5 8.137448928521192 6.542383193926052E-4 86-87 0.0016192398404966976 0.0 3.2711915969630255E-5 8.58805557100285 6.542383193926052E-4 88-89 0.001651951756466328 0.0 3.2711915969630255E-5 9.043241881720254 6.542383193926052E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5635 0.0 52.255764 1 GTATCAA 9845 0.0 41.80672 1 ATCAACG 12770 0.0 31.459827 3 TATCAAC 12850 0.0 31.335135 2 TCAACGC 12940 0.0 31.26516 4 CAACGCA 13170 0.0 30.686443 5 AACGCAG 13715 0.0 29.501343 6 ACGCAGA 15300 0.0 25.983906 7 CGCAGAG 15320 0.0 25.919703 8 TACATGG 11445 0.0 25.35889 2 GTACATG 11590 0.0 25.24415 1 TGGTATC 2645 0.0 24.364403 2 ACATGGG 11860 0.0 23.917841 3 GCAGAGT 16315 0.0 23.271946 9 GAGTACT 9675 0.0 21.85712 12-13 GTGGTAT 2995 0.0 21.67385 1 AGAGTAC 14650 0.0 20.8577 10-11 CAGAGTA 15695 0.0 20.772884 10-11 GTACTTT 10360 0.0 20.439163 14-15 AGTACTT 10225 0.0 19.92227 12-13 >>END_MODULE