FastQCFastQC Report
Fri 27 May 2016
SRR939874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056990
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98710.3228993225362203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85940.28112620584300246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56880.1860653780352569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55950.053.513981
GTATCAA99800.042.220211
ATCAACG127350.032.5521743
TATCAAC129650.032.1618232
TCAACGC129000.032.0990374
CAACGCA131000.031.5727675
AACGCAG137250.030.100476
ACGCAGA157850.025.9619087
TGGTATC26150.025.9411162
CGCAGAG159300.025.4873968
GTGGTAT28650.025.0086541
TACATGG112150.023.6448632
GTACATG116150.023.5310481
GCAGAGT176600.022.6145979
GTATAGG8050.022.3988481
ACATGGG114100.022.3647523
GAGTACT105600.020.99869312-13
CAGAGTA168350.020.04623210-11
AGAGTAC159600.020.00106610-11
GTACTTT113050.019.46802914-15
Produced by FastQC (version 0.11.5)