##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939874_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3056990 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405477283209954 31.0 31.0 34.0 30.0 34.0 2 31.63810611091302 31.0 31.0 34.0 30.0 34.0 3 31.676135348823514 31.0 31.0 34.0 30.0 34.0 4 35.490755285427824 37.0 35.0 37.0 33.0 37.0 5 35.231201933928475 37.0 35.0 37.0 32.0 37.0 6 35.256889293062784 37.0 35.0 37.0 32.0 37.0 7 35.160023748850996 37.0 35.0 37.0 32.0 37.0 8 35.123411918259464 37.0 35.0 37.0 32.0 37.0 9 36.70099411512632 39.0 35.0 39.0 32.0 39.0 10-11 36.567239343275574 38.5 35.0 39.0 32.0 39.0 12-13 36.48920474061086 38.0 35.0 39.0 32.0 39.0 14-15 37.52420894409207 39.0 36.0 41.0 32.0 41.0 16-17 37.44509174056834 39.0 36.0 41.0 32.0 41.0 18-19 37.431867948537615 39.0 36.0 41.0 32.0 41.0 20-21 37.34552451921662 39.0 36.0 41.0 31.5 41.0 22-23 37.22400465817684 39.0 36.0 40.0 31.0 41.0 24-25 37.07498029107063 39.0 36.0 40.0 31.0 41.0 26-27 36.77709691559345 39.0 35.5 40.0 30.5 41.0 28-29 36.552188427178365 38.0 35.0 40.0 30.0 41.0 30-31 36.27124655298185 38.0 35.0 40.0 30.0 41.0 32-33 36.238385797794564 38.0 35.0 40.0 30.0 41.0 34-35 36.0889430452831 38.0 35.0 40.0 30.0 41.0 36-37 36.11929904906461 38.0 35.0 40.0 30.0 41.0 38-39 36.00912204488729 38.0 35.0 40.0 29.0 41.0 40-41 35.9082502723267 38.0 35.0 40.0 29.0 41.0 42-43 35.67620551588327 38.0 34.5 40.0 28.0 41.0 44-45 35.42895053631186 38.0 34.0 40.0 27.0 41.0 46-47 35.129809060546485 38.0 34.0 40.0 26.5 41.0 48-49 34.80998056912192 38.0 33.0 40.0 25.5 41.0 50-51 34.578777980955124 38.0 33.0 40.0 24.5 41.0 52-53 34.32452019797252 38.0 33.0 40.0 24.0 41.0 54-55 34.048398424594126 37.0 33.0 40.0 24.0 41.0 56-57 33.68235372048976 37.0 32.5 40.0 23.0 41.0 58-59 33.31071756858871 36.5 32.0 39.5 22.5 41.0 60-61 32.994300275761454 36.0 31.0 39.0 22.0 40.5 62-63 32.69910680113445 36.0 31.0 39.0 21.0 40.0 64-65 32.26534597757926 35.0 31.0 39.0 19.5 40.0 66-67 31.353049895485427 35.0 29.5 38.0 14.0 40.0 68-69 29.434647152918394 34.0 26.0 37.0 7.5 39.5 70-71 28.787300579982272 33.5 25.5 36.5 7.5 39.0 72-73 29.358371142856207 34.0 27.0 36.0 7.5 39.0 74-75 29.471023294155362 34.0 28.0 36.0 8.0 39.0 76-77 29.029506638883348 32.5 27.5 35.0 8.0 37.5 78-79 29.56471545539894 34.0 29.0 35.0 7.0 37.5 80-81 29.383450878151386 34.0 29.0 35.0 7.0 37.0 82-83 28.93846446995247 33.5 28.5 35.0 2.0 36.5 84-85 28.57209542720127 33.0 28.0 35.0 2.0 36.0 86-87 28.19103088331987 33.0 27.0 35.0 2.0 36.0 88-89 27.734342932099874 33.0 26.5 35.0 2.0 35.0 90-91 27.24211773672796 33.0 26.0 35.0 2.0 35.0 92-93 26.7500657509511 32.0 25.0 34.5 2.0 35.0 94-95 25.934654676659065 32.0 24.0 34.0 2.0 35.0 96-97 25.403131348156194 32.0 22.0 34.0 2.0 35.0 98-99 24.727254914147576 31.5 19.0 34.0 2.0 35.0 100-101 23.250058554329584 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2919214318323924 1101 2 0.359258397636971 1101 3 0.3271898019574664 1101 4 0.27324146614894573 1101 5 0.35391665984155196 1101 6 0.3207786947484408 1101 7 0.23338641319742237 1101 8 0.152309143061224 1101 9 0.2787450951241084 1101 10-11 0.3537558679013486 1101 12-13 0.43622202116100794 1101 14-15 0.44207055595877875 1101 16-17 0.4925002114058188 1101 18-19 0.34321846922831156 1101 20-21 0.3983376477092051 1101 22-23 0.36318332959638866 1101 24-25 0.3565837999274777 1101 26-27 0.27168034968730836 1101 28-29 0.4529433948484325 1101 30-31 0.3052677542443192 1101 32-33 0.3279489299307059 1101 34-35 0.2502152452351538 1101 36-37 0.38172853546560503 1101 38-39 0.47533855476697084 1101 40-41 0.440000027359595 1101 42-43 0.39041642926612496 1101 44-45 0.1777717490919457 1101 46-47 0.2598964178754031 1101 48-49 0.41412600225381624 1101 50-51 0.4088769327188544 1101 52-53 0.2237441182436939 1101 54-55 0.09654030787063306 1101 56-57 0.047458709565994184 1101 58-59 0.15063324246033716 1101 60-61 0.10076191133730816 1101 62-63 0.07810060295557975 1101 64-65 -9.814374902248346E-4 1101 66-67 9.311830988352199E-4 1101 68-69 -8.570286193292969 1101 70-71 -6.463861460566452 1101 72-73 -1.6000179523180122 1101 74-75 -0.3774308920717324 1101 76-77 -0.24741285599091256 1101 78-79 -0.31859521509282374 1101 80-81 -0.29637695880302317 1101 82-83 -0.050539156315320355 1101 84-85 -0.009136915436712911 1101 86-87 -0.12737889309411443 1101 88-89 -0.08579742274049451 1101 90-91 -0.29796336333556894 1101 92-93 -0.4019475993259043 1101 94-95 0.04651017485698716 1101 96-97 -0.1889790410303469 1101 98-99 -0.14553186809429164 1101 100-101 -0.007009819566217601 1102 1 0.10271155079460215 1102 2 0.03661788623865547 1102 3 0.1596535297256665 1102 4 0.1298049465699691 1102 5 0.16778330652056894 1102 6 0.06836807702296 1102 7 0.18155829271471902 1102 8 0.05617975157809951 1102 9 0.150473420123312 1102 10-11 0.19287130729058788 1102 12-13 0.05309645231604776 1102 14-15 0.2681259574428623 1102 16-17 0.2422544703815248 1102 18-19 0.1455491006596148 1102 20-21 0.1085297433072796 1102 22-23 0.10776985292304886 1102 24-25 0.05767191334312116 1102 26-27 0.21415735496618993 1102 28-29 0.03032772524429106 1102 30-31 0.018880200839809902 1102 32-33 -0.13148955985952426 1102 34-35 -0.1001087688561384 1102 36-37 -0.1820080130240811 1102 38-39 0.14638812057095407 1102 40-41 0.16970192582947874 1102 42-43 0.10213297582843239 1102 44-45 0.08623916029858947 1102 46-47 -0.04029043096442564 1102 48-49 0.02611848546954576 1102 50-51 -0.09707942281862358 1102 52-53 -0.1128255726258729 1102 54-55 -0.15510578222370697 1102 56-57 -0.15901054335987652 1102 58-59 -0.2352013668703279 1102 60-61 -0.1379745836140316 1102 62-63 -0.10297144895520915 1102 64-65 -0.1475359234685314 1102 66-67 -0.01691496909998591 1102 68-69 -5.061699605720289 1102 70-71 -0.26326482919940375 1102 72-73 0.210055502297525 1102 74-75 0.05423361521483727 1102 76-77 -0.17472662284210472 1102 78-79 -0.44953866788805996 1102 80-81 -0.2813564536151567 1102 82-83 -0.2872304863149999 1102 84-85 -0.4135556495759758 1102 86-87 -0.6108463578391081 1102 88-89 -0.6066617458551455 1102 90-91 -0.7147707644770058 1102 92-93 -0.6634741918241076 1102 94-95 -0.6217558250787683 1102 96-97 -1.2387761306856682 1102 98-99 -0.8268075798866512 1102 100-101 -0.724364275563044 1103 1 0.16384012035697992 1103 2 0.20100790583369132 1103 3 0.17647767080992693 1103 4 0.18155622024730178 1103 5 0.21171600410384883 1103 6 0.0778960717976176 1103 7 0.15743100336135285 1103 8 0.20033274961859604 1103 9 0.26128935741919435 1103 10-11 0.30877881872102364 1103 12-13 0.2502810375544584 1103 14-15 0.2306738346473054 1103 16-17 0.30007529337303396 1103 18-19 0.24405125611356482 1103 20-21 0.3008740411518218 1103 22-23 0.17959119844885407 1103 24-25 0.19228740648485854 1103 26-27 0.2424095300151734 1103 28-29 0.01588503419269216 1103 30-31 0.054950377195133626 1103 32-33 0.20010761845529856 1103 34-35 -0.012889017059926289 1103 36-37 0.217725912514787 1103 38-39 0.24528609575057914 1103 40-41 0.18913445358911218 1103 42-43 0.022568560413674277 1103 44-45 0.07122617532145625 1103 46-47 -0.002407844419678895 1103 48-49 -0.07519203053306711 1103 50-51 -0.11571978859262089 1103 52-53 -0.09628866864155583 1103 54-55 -0.18846034786707833 1103 56-57 -0.13507899535203904 1103 58-59 -0.16229790508064212 1103 60-61 -0.20782038374466794 1103 62-63 -0.3251046429460658 1103 64-65 -0.3833211096213702 1103 66-67 -0.23327865296673878 1103 68-69 -4.334221930997888 1103 70-71 0.06875230992535108 1103 72-73 0.31083188374756077 1103 74-75 0.18760255055121888 1103 76-77 -0.0710823641876317 1103 78-79 -0.4660929200108548 1103 80-81 -0.30383138503253093 1103 82-83 -0.3977968612333562 1103 84-85 -0.2712772433055619 1103 86-87 -0.43665364719247535 1103 88-89 -0.5332505374945917 1103 90-91 -0.47873188792573274 1103 92-93 -0.3969364517849172 1103 94-95 -0.44783080874957193 1103 96-97 -0.741456418079526 1103 98-99 -0.17726432711068796 1103 100-101 -0.17548850809080463 1104 1 0.1731672920741545 1104 2 0.23322780482359207 1104 3 0.16811110515336125 1104 4 0.14335116974225315 1104 5 0.16823277178061602 1104 6 0.23344718290873345 1104 7 0.15283272053306973 1104 8 0.14917658800244027 1104 9 0.25957925640908996 1104 10-11 0.24029927326647282 1104 12-13 0.19902194664535955 1104 14-15 0.25780691545539014 1104 16-17 0.2836463034740433 1104 18-19 0.24139014500244826 1104 20-21 0.24731616236395126 1104 22-23 0.11915513784278886 1104 24-25 0.08833760850505712 1104 26-27 0.2791777623384064 1104 28-29 0.1645962968189565 1104 30-31 0.21513714487190327 1104 32-33 0.25065312350580626 1104 34-35 0.285182851626935 1104 36-37 0.2434437407976091 1104 38-39 0.3255580654475452 1104 40-41 0.3410935444982002 1104 42-43 0.2915784594035671 1104 44-45 0.32833496320024125 1104 46-47 0.22582362022678382 1104 48-49 0.21246554522451078 1104 50-51 0.2629087467609139 1104 52-53 0.34553456368168156 1104 54-55 0.29583116728444026 1104 56-57 0.25385484303179595 1104 58-59 0.23464477168703723 1104 60-61 0.2772319394876632 1104 62-63 0.2555461651347386 1104 64-65 0.30206252674226874 1104 66-67 0.47780670056861396 1104 68-69 -3.9038964764524344 1104 70-71 0.8280885725516143 1104 72-73 1.1438136009192768 1104 74-75 0.7164057323693989 1104 76-77 0.7370331408628736 1104 78-79 0.42335647392853915 1104 80-81 0.31760528163413326 1104 82-83 0.34570546199897123 1104 84-85 0.24649336275504297 1104 86-87 0.43208211038328415 1104 88-89 0.22416117967712168 1104 90-91 0.13282412217527906 1104 92-93 0.14012041690195076 1104 94-95 0.4643290397352793 1104 96-97 0.024128985960878424 1104 98-99 0.3145424405660826 1104 100-101 0.2567569464546473 1105 1 0.23848606032538555 1105 2 0.1759144461180533 1105 3 0.2011353643328313 1105 4 0.10224879528679764 1105 5 0.21757812100748453 1105 6 0.20389215394612137 1105 7 0.1714528675003706 1105 8 0.22116004819680057 1105 9 0.24532474446500885 1105 10-11 0.31121294194376503 1105 12-13 0.20062416551654394 1105 14-15 0.2609196482334042 1105 16-17 0.31293115435249774 1105 18-19 0.22717542672967994 1105 20-21 0.2758374534741037 1105 22-23 0.28994089829087244 1105 24-25 0.2989260162794878 1105 26-27 0.25869793443855116 1105 28-29 0.29481659817373185 1105 30-31 0.3441922097385799 1105 32-33 0.2623334636369776 1105 34-35 0.3433670334929886 1105 36-37 0.1344786455321625 1105 38-39 0.39853980823082935 1105 40-41 0.33643050414202946 1105 42-43 0.15726075630624337 1105 44-45 0.11530807105604879 1105 46-47 0.0217369897035411 1105 48-49 0.11263608953012039 1105 50-51 0.17902144363486627 1105 52-53 -0.0013818755925782966 1105 54-55 0.07797428088489511 1105 56-57 -0.08096461936467136 1105 58-59 -8.659935482597803E-4 1105 60-61 0.16815838402784777 1105 62-63 0.20527538864951111 1105 64-65 0.16737740538652446 1105 66-67 0.5120774291817618 1105 68-69 1.7806378478330736 1105 70-71 1.885061441046993 1105 72-73 1.0886778237159653 1105 74-75 0.5472405916254672 1105 76-77 0.15371940516465799 1105 78-79 0.09585665344886252 1105 80-81 0.275296829817389 1105 82-83 0.15672471854547965 1105 84-85 -0.010544731457223122 1105 86-87 -0.08201023487019654 1105 88-89 -0.11299473970628426 1105 90-91 -0.026699281290660792 1105 92-93 -0.026799074217777274 1105 94-95 0.29629936502609766 1105 96-97 -0.09553641807952573 1105 98-99 0.24191899042486043 1105 100-101 0.11348716331519881 1106 1 0.37397889913576066 1106 2 0.3237795307919846 1106 3 0.34353368682594265 1106 4 0.21005992395100748 1106 5 0.2927398546210327 1106 6 0.33058598838086795 1106 7 0.3547571828742022 1106 8 0.3063925160517016 1106 9 0.39084878351195584 1106 10-11 0.40652563220116633 1106 12-13 0.46964199851541366 1106 14-15 0.6103436377837781 1106 16-17 0.5015754068198106 1106 18-19 0.4968435150391528 1106 20-21 0.5258892430203659 1106 22-23 0.44751853912286066 1106 24-25 0.5463628686136488 1106 26-27 0.5637412083601916 1106 28-29 0.5269231122707723 1106 30-31 0.6861678346525935 1106 32-33 0.7100097339040801 1106 34-35 0.7830865318222848 1106 36-37 0.8329172104794154 1106 38-39 0.8538434531079346 1106 40-41 0.7652724582604478 1106 42-43 0.653578717237167 1106 44-45 0.9752764122250213 1106 46-47 0.8553703070651366 1106 48-49 0.8827248196504485 1106 50-51 0.8596091403363033 1106 52-53 0.9034390590861747 1106 54-55 0.8901015740548459 1106 56-57 0.8976302472238658 1106 58-59 0.8866902831075478 1106 60-61 0.9827992492216353 1106 62-63 1.0479721138106939 1106 64-65 0.909546905059976 1106 66-67 1.524890716402627 1106 68-69 3.0123246689353813 1106 70-71 3.1686592568722993 1106 72-73 2.180760585782931 1106 74-75 1.5524960840847513 1106 76-77 1.1982562010442663 1106 78-79 1.150548628204028 1106 80-81 1.1764120050241935 1106 82-83 1.2464158314593377 1106 84-85 1.3758035441485603 1106 86-87 1.1790525656870727 1106 88-89 1.37377291262219 1106 90-91 1.3087268241196455 1106 92-93 1.2126560206875538 1106 94-95 1.565298854246759 1106 96-97 1.363722841179733 1106 98-99 1.5190357596427368 1106 100-101 1.474946727144431 1107 1 0.0729778981347593 1107 2 0.06548790583369168 1107 3 0.013549416841669881 1107 4 0.016832410723495173 1107 5 0.018413146960988058 1107 6 0.009240833702378382 1107 7 -0.05967629822594489 1107 8 0.08440386072970796 1107 9 -0.08825984893001504 1107 10-11 -0.013938641596439538 1107 12-13 0.028839767713179754 1107 14-15 0.031969072742541016 1107 16-17 0.00971592829367296 1107 18-19 0.04379093865324535 1107 20-21 -0.03851897472119248 1107 22-23 0.12001913495678451 1107 24-25 0.035186454103900644 1107 26-27 0.07698349826913642 1107 28-29 0.04725773260539512 1107 30-31 -0.03652771804296151 1107 32-33 -0.025915238687552744 1107 34-35 0.26658526865435306 1107 36-37 0.06241289664176719 1107 38-39 0.09394641321089381 1107 40-41 0.00536302501767949 1107 42-43 0.028818719143828275 1107 44-45 0.1736109372262149 1107 46-47 0.08245691748508222 1107 48-49 0.08190638216534296 1107 50-51 0.11972783045499114 1107 52-53 0.24713990278701914 1107 54-55 0.25944949340276224 1107 56-57 0.14045116337812402 1107 58-59 0.2507243171415823 1107 60-61 0.2561821559378785 1107 62-63 0.28137408721266155 1107 64-65 0.05931000148974164 1107 66-67 0.7329816644935825 1107 68-69 1.990010449954493 1107 70-71 2.2502837384967798 1107 72-73 1.2201537885094673 1107 74-75 0.6749942965829661 1107 76-77 0.39651636597109885 1107 78-79 0.43884168316374783 1107 80-81 0.3246092498881019 1107 82-83 0.5289612340047398 1107 84-85 0.48136529637697834 1107 86-87 0.433474606775782 1107 88-89 0.34381485933080214 1107 90-91 0.2582687469949043 1107 92-93 0.13272323075476677 1107 94-95 0.5288104610916982 1107 96-97 0.12954294699983748 1107 98-99 0.41815535542899696 1107 100-101 0.6749495871472924 1108 1 -0.19144534864143026 1108 2 -0.14134834217902537 1108 3 -0.15801492060501943 1108 4 -0.08855633937113794 1108 5 -0.05083509287548793 1108 6 -0.03358602677153755 1108 7 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48.0 5 153.0 6 294.0 7 786.0 8 1755.0 9 3044.0 10 5351.0 11 8029.0 12 11185.0 13 14274.0 14 17744.0 15 21650.0 16 26069.0 17 30457.0 18 28002.0 19 23999.0 20 23965.0 21 24279.0 22 26532.0 23 30047.0 24 36371.0 25 44556.0 26 55085.0 27 68138.0 28 83167.0 29 101266.0 30 119979.0 31 143641.0 32 172257.0 33 208804.0 34 259541.0 35 326717.0 36 409553.0 37 447661.0 38 260318.0 39 22247.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.96343471670036 19.776676732257148 14.882437415072602 23.37745113596989 2 15.89668418262844 20.705797281644887 39.58737149326301 23.810147042463665 3 19.218099645795142 24.556753248622503 30.3127457484295 25.91240135715286 4 13.214599982335566 17.193023202561996 36.96037605618599 32.632000758916455 5 13.342012895037275 37.78200124959519 34.64231155483008 14.233674300537459 6 32.08435885256991 36.851371349838466 17.040008007882268 14.024261789709346 7 28.2401970565818 32.91626730869254 20.734971328005653 18.10856430672001 8 26.228741343609236 35.72805275777808 19.379225970644328 18.66397992796836 9 26.141400527970323 16.636658935750525 19.97520436769502 37.246736168584135 10-11 24.922979793849507 26.638932413910414 27.515448202316655 20.922639589923424 12-13 25.79851422477666 23.88748409383086 27.942436841468243 22.371564839924236 14-15 22.473871357119258 25.525271590682337 26.005498873074494 25.99535817912391 16-17 21.94664341932234 28.674394336894583 26.882838618505843 22.496123625277235 18-19 21.92102362127452 27.044282120648088 29.29010235558507 21.74459190249232 20-21 22.55815360861501 26.28850601408575 29.44615455071819 21.70718582658105 22-23 22.241289634575185 26.246258574610977 29.334148950438177 22.178302840375665 24-25 22.05633092829757 26.52100261417405 29.411756046841525 22.010910410686858 26-27 21.83993680037161 26.784800685644 29.22598446833149 22.1492780456529 28-29 21.799640397731295 27.03724596935841 29.201009424798297 21.962104208112006 30-31 22.07539220156926 26.651109888405987 29.081585253914493 22.191912656110258 32-33 21.74030594979063 26.693364392720238 29.086117013437786 22.480212644051345 34-35 22.328452539844548 26.882638507236134 28.906352228415898 21.882556724503416 36-37 22.427672527610703 26.408696062200054 29.018467605884695 22.145163804304545 38-39 21.954932983600457 26.79819412368226 28.902927026441645 22.343945866275636 40-41 22.31227605131551 26.652318669614854 29.00619790903457 22.02920737003506 42-43 22.063890471606054 26.639553084333723 28.841549272714172 22.455007171346054 44-45 22.113813231801615 26.836275847481073 28.87152707856298 22.17838384215433 46-47 22.369619134150234 26.96220808879503 28.35639155595937 22.311781221095366 48-49 22.10777291069434 27.152710349927418 28.23604159596463 22.503475143413613 50-51 22.174945116830347 27.24050206026753 28.176552731225534 22.408000091676588 52-53 22.452313587693602 27.03451059496183 28.14983692882122 22.363338888523344 54-55 22.06520260865424 27.154595446327555 28.242762153008776 22.53743979200943 56-57 22.042872912845954 27.35001524058279 28.050722099303155 22.556389747268106 58-59 22.07219733773217 26.99746153118842 28.05901195687842 22.87132917420099 60-61 21.919169927403654 27.18220471021575 28.27369351193355 22.624931850447044 62-63 21.889450744143808 27.11677640617926 28.36374395800239 22.630028891674538 64-65 21.66155871376092 27.12758901406013 28.211553487766512 22.999298784412435 66-67 21.528919446477516 27.267809977112385 28.21879588743724 22.98447468897286 68-69 21.655527073350513 26.919346234852497 28.555056569513297 22.870070122283696 70-71 21.53364880473253 26.96695891942343 28.32009345257272 23.179298823271317 72-73 21.57355656817396 27.118314137824246 28.12565644053161 23.182472853470184 74-75 21.812790802116886 27.46048919626013 27.528680804863853 23.198039196759133 76-77 21.700813841872566 27.30834353573825 27.700564174040405 23.29027844834878 78-79 21.90620224308158 27.46737092761223 27.439024489980852 23.18740233932534 80-81 21.927237695211552 27.42880135036998 27.251107316466793 23.392853637951678 82-83 21.938695232465673 27.554449819740928 27.243801008116158 23.263053939677246 84-85 21.870036967893444 27.542389098773068 27.224035378741807 23.363538554591674 86-87 21.80879487005441 27.452700033775468 27.23419811070012 23.504306985470006 88-89 21.820569793906248 27.549994152730672 26.971973179434293 23.657462873928782 90-91 21.69299067147884 27.530799298259158 27.1106118511506 23.6655981791114 92-93 21.76207982162415 27.33851698901769 27.191964057893642 23.707439131464522 94-95 21.447847916403063 27.15785765741906 27.413291289770424 23.981003136407452 96-97 21.718433738861922 26.837372669426614 27.359037659046148 24.085155932665316 98-99 21.49125002576553 27.088079507683688 27.054805165146416 24.36586530140436 100-101 21.3430000336967 27.091150232000157 27.26138400532081 24.304465728982336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 628.0 1 450.5 2 313.5 3 394.5 4 391.5 5 385.5 6 640.0 7 1332.0 8 2187.0 9 2623.0 10 2394.0 11 2173.5 12 2264.0 13 2433.5 14 2685.0 15 2937.0 16 3240.5 17 3529.0 18 3949.0 19 4518.5 20 5113.5 21 6037.0 22 7291.5 23 8950.0 24 11152.5 25 14017.0 26 18388.0 27 23730.0 28 27898.0 29 31886.5 30 36965.0 31 43946.0 32 52815.5 33 58343.0 34 62276.5 35 67833.5 36 74960.0 37 81437.0 38 84606.0 39 86826.5 40 90092.0 41 94579.5 42 97771.5 43 100753.5 44 103841.0 45 108025.0 46 110943.5 47 112343.5 48 113964.5 49 112404.0 50 110222.5 51 108309.5 52 106046.5 53 105436.5 54 104371.0 55 98017.5 56 88892.0 57 79998.0 58 72938.5 59 64613.0 60 53375.0 61 43549.5 62 35168.5 63 26402.5 64 18948.5 65 14112.0 66 10178.5 67 7512.5 68 5543.0 69 4033.5 70 2969.0 71 2082.5 72 1539.0 73 1254.5 74 991.5 75 744.5 76 508.5 77 325.5 78 211.5 79 126.5 80 78.0 81 55.5 82 34.5 83 19.0 84 14.5 85 8.0 86 3.5 87 4.0 88 2.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02299647692665007 2 0.023879698657830087 3 6.542383193926052E-5 4 0.0 5 0.0 6 6.542383193926052E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.271191596963026E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.906787395444539E-5 26-27 3.271191596963026E-4 28-29 4.906787395444539E-5 30-31 5.233906555140842E-4 32-33 0.001651951756466328 34-35 0.0035983107566593285 36-37 0.10147236333779305 38-39 0.09321260455546142 40-41 0.10263363635471492 42-43 0.10369677362372792 44-45 0.09712167851383223 46-47 0.09044844765602766 48-49 0.09157700875697991 50-51 0.09077556681572396 52-53 0.07160638405752064 54-55 0.06056611241777042 56-57 0.032859119591493596 58-59 0.0138371404551536 60-61 0.00960094733708648 62-63 0.0017664434623600339 64-65 0.005086702933277505 66-67 0.15322261440174814 68-69 0.4119738697215235 70-71 0.3932953657028646 72-73 0.17975197825311826 74-75 0.029293520750803895 76-77 0.030405725893771322 78-79 0.005511957840882698 80-81 0.0016355957984815128 82-83 3.271191596963026E-5 84-85 4.2525490760519333E-4 86-87 0.0012266968488611346 88-89 2.453393697722269E-4 90-91 6.869502353622353E-4 92-93 0.0024206817817526392 94-95 0.07787071596570483 96-97 0.01722282375801033 98-99 0.01900562317835518 100-101 0.01017340586655501 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3056990.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.84840725497231 #Duplication Level Percentage of deduplicated Percentage of total 1 72.41400597730971 36.82136866898244 2 14.670172427991726 14.919098042383784 3 5.364633795101409 8.183492519613122 4 2.564359747208055 5.21574435097172 5 1.3842563699747739 3.519361582288346 6 0.8384504792243681 2.558032285843642 7 0.5346641166959749 1.9030773125263876 8 0.37674609418569327 1.5325551063099427 9 0.28199543629092677 1.2905116909708176 >10 1.4164738020697718 12.946793315960589 >50 0.09994033403481549 3.494528309537712 >100 0.049669820396974956 4.5306554254460965 >500 0.002701766384475414 0.9222157876314141 >1k 0.0017368498180876865 1.372529497624756 >5k 1.9298331312085404E-4 0.7900361039091235 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9871 0.3228993225362203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8594 0.28112620584300246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5688 0.1860653780352569 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.542383193926051E-5 2 0.0 0.0 0.0 0.0 6.542383193926051E-5 3 0.0 0.0 0.0 0.0 6.542383193926051E-5 4 0.0 0.0 0.0 0.0 6.542383193926051E-5 5 3.2711915969630255E-5 0.0 0.0 0.0 6.542383193926051E-5 6 6.542383193926051E-5 0.0 0.0 0.0 6.542383193926051E-5 7 9.813574790889077E-5 0.0 0.0 6.542383193926051E-5 6.542383193926051E-5 8 9.813574790889077E-5 0.0 0.0 6.542383193926051E-5 6.542383193926051E-5 9 9.813574790889077E-5 0.0 0.0 1.635595798481513E-4 6.542383193926051E-5 10-11 1.3084766387852102E-4 0.0 0.0 1.9627149581778156E-4 6.542383193926051E-5 12-13 1.3084766387852102E-4 0.0 0.0 3.271191596963026E-4 3.1076320171148744E-4 14-15 2.289834117874118E-4 0.0 0.0 3.271191596963026E-4 3.5983107566593285E-4 16-17 2.6169532775704204E-4 0.0 0.0 3.7618703365074793E-4 4.4161086559000846E-4 18-19 3.4347511768111766E-4 0.0 0.0 4.4161086559000846E-4 4.906787395444538E-4 20-21 3.9254299163556306E-4 0.0 0.0 6.051704454381598E-4 5.233906555140841E-4 22-23 3.9254299163556306E-4 0.0 0.0 0.0010958491849826137 5.561025714837144E-4 24-25 4.579668235748236E-4 0.0 0.0 0.0016355957984815128 5.561025714837144E-4 26-27 4.7432278155963873E-4 0.0 0.0 0.0025024615716767147 5.561025714837144E-4 28-29 6.3788236140779E-4 0.0 0.0 0.007147553639364211 5.561025714837144E-4 30-31 7.52374067301496E-4 0.0 0.0 0.02296376501068044 5.561025714837144E-4 32-33 7.68730025286311E-4 0.0 0.0 0.05938848344286374 5.561025714837144E-4 34-35 8.014419412559412E-4 0.0 0.0 0.11432814631385775 5.888144874533446E-4 36-37 9.159336471496472E-4 0.0 0.0 0.2100759243569655 5.888144874533446E-4 38-39 9.977134370737228E-4 0.0 0.0 0.35736132601022574 6.215264034229749E-4 40-41 0.001014069395058538 0.0 0.0 0.514378522664451 6.542383193926052E-4 42-43 0.0011449170589370589 0.0 0.0 0.671591990814494 6.542383193926052E-4 44-45 0.0012594087648307647 0.0 0.0 0.8636272935142084 6.542383193926052E-4 46-47 0.0013739004707244706 0.0 0.0 1.09131858462082 6.705942773774203E-4 48-49 0.0014393243026637314 0.0 0.0 1.3321109980732682 6.869502353622354E-4 50-51 0.0014393243026637314 0.0 0.0 1.5866424162329613 7.196621513318657E-4 52-53 0.0014393243026637314 0.0 0.0 1.8320962777110816 7.850859832711261E-4 54-55 0.0014556802606485466 0.0 0.0 2.096686610031436 7.850859832711261E-4 56-57 0.0014720362186333615 0.0 0.0 2.3945449608929046 7.850859832711261E-4 58-59 0.0014720362186333615 0.0 0.0 2.6992564581500105 7.850859832711261E-4 60-61 0.0014883921766181767 1.6355957984815128E-5 0.0 3.012178646315493 8.177978992407564E-4 62-63 0.001504748134602992 3.2711915969630255E-5 0.0 3.3110183546560505 8.505098152103867E-4 64-65 0.001504748134602992 3.2711915969630255E-5 0.0 3.619720705661451 8.505098152103867E-4 66-67 0.0015701719665422522 3.2711915969630255E-5 0.0 3.958943274266517 8.505098152103867E-4 68-69 0.0015701719665422522 3.2711915969630255E-5 0.0 4.339333789119363 8.505098152103867E-4 70-71 0.0015865279245270674 3.2711915969630255E-5 0.0 4.7627568294302565 8.505098152103867E-4 72-73 0.0016028838825118826 3.2711915969630255E-5 0.0 5.198070651196112 8.832217311800169E-4 74-75 0.0016028838825118826 3.2711915969630255E-5 0.0 5.62857582131443 9.650015211040925E-4 76-77 0.0016028838825118826 3.2711915969630255E-5 0.0 6.093608418738694 9.813574790889076E-4 78-79 0.0016192398404966976 3.2711915969630255E-5 0.0 6.613809662445739 9.813574790889076E-4 80-81 0.001946359000193 3.2711915969630255E-5 0.0 7.1637787496851475 9.977134370737228E-4 82-83 0.0019627149581778153 3.2711915969630255E-5 0.0 7.724935312186171 0.001030425353043353 84-85 0.0020281387901170756 3.2711915969630255E-5 0.0 8.260511156398941 0.0010794932269977985 86-87 0.0021262745380259667 3.2711915969630255E-5 0.0 8.806767441175797 0.0011285611009522439 88-89 0.002158986453995597 3.2711915969630255E-5 0.0 9.388123611788066 0.001144917058937059 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5595 0.0 53.51398 1 GTATCAA 9980 0.0 42.22021 1 ATCAACG 12735 0.0 32.552174 3 TATCAAC 12965 0.0 32.161823 2 TCAACGC 12900 0.0 32.099037 4 CAACGCA 13100 0.0 31.572767 5 AACGCAG 13725 0.0 30.10047 6 ACGCAGA 15785 0.0 25.961908 7 TGGTATC 2615 0.0 25.941116 2 CGCAGAG 15930 0.0 25.487396 8 GTGGTAT 2865 0.0 25.008654 1 TACATGG 11215 0.0 23.644863 2 GTACATG 11615 0.0 23.531048 1 GCAGAGT 17660 0.0 22.614597 9 GTATAGG 805 0.0 22.398848 1 ACATGGG 11410 0.0 22.364752 3 GAGTACT 10560 0.0 20.998693 12-13 CAGAGTA 16835 0.0 20.046232 10-11 AGAGTAC 15960 0.0 20.001066 10-11 GTACTTT 11305 0.0 19.468029 14-15 >>END_MODULE