FastQCFastQC Report
Fri 27 May 2016
SRR939873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484637
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.31815512688573827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.2473198298181988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.15716581536860313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.042.5535961
GTATCAA104800.034.4551961
TATCAAC129550.027.9825522
ATCAACG130300.027.7133563
TCAACGC130650.027.4575184
CAACGCA131850.027.171635
AACGCAG136350.026.2748766
TACATGG103400.023.9082932
GTACATG103450.023.850871
ACGCAGA150450.023.6862687
CGCAGAG153300.023.1221058
GAGTACT82100.022.39658512-13
ACATGGG105500.022.1289863
GCAGAGT157350.021.2000779
GTACTTT87900.020.78485514-15
AGGTAAG13400.020.183732
AGAGTAC136650.019.9318110-11
AGTACTT86950.019.89212612-13
CATGGGG49200.019.3856264
CAGAGTA153450.019.29577410-11
Produced by FastQC (version 0.11.5)