FastQCFastQC Report
Fri 27 May 2016
SRR939873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484637
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87540.35232510825525015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75990.3058394445546774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.20453692028252016No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25200.1014232662557951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60350.043.3826451
GTATCAA99450.036.240061
TATCAAC126050.028.435272
ATCAACG126050.028.3575843
TCAACGC126750.028.2004074
CAACGCA128950.027.6827145
AACGCAG131100.027.3201276
GTACATG103800.025.5865291
TACATGG102100.025.3590622
ACATGGG100550.024.856313
ACGCAGA147750.024.1138277
CGCAGAG147850.024.0337688
CATGGGA56100.021.6865754
GCAGAGT161450.021.4546369
GAGTACT90050.021.01251612-13
AGAGTAC138200.020.85260410-11
AGTACTT94200.020.18686512-13
TAAGGTG15200.019.8550285
GTACTTT94400.019.79483214-15
CATGGGG47200.019.2818834
Produced by FastQC (version 0.11.5)