##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939873_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2484637 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.476933652682465 31.0 31.0 34.0 30.0 34.0 2 31.690815600025275 31.0 31.0 34.0 30.0 34.0 3 31.744674574193333 31.0 31.0 34.0 30.0 34.0 4 35.551761887148906 37.0 35.0 37.0 33.0 37.0 5 35.28449346926734 37.0 35.0 37.0 33.0 37.0 6 35.31640316070315 37.0 35.0 37.0 32.0 37.0 7 35.23627918283435 37.0 35.0 37.0 32.0 37.0 8 35.17219296017889 37.0 35.0 37.0 32.0 37.0 9 36.785038619323466 39.0 37.0 39.0 32.0 39.0 10-11 36.6442379711805 39.0 35.0 39.0 32.0 39.0 12-13 36.58959679019511 39.0 35.0 39.0 32.0 39.0 14-15 37.64150034793815 39.0 36.0 41.0 32.0 41.0 16-17 37.568231697427024 39.0 36.0 41.0 32.0 41.0 18-19 37.55405779596778 39.0 36.0 41.0 32.0 41.0 20-21 37.48140734441289 39.0 36.0 41.0 32.0 41.0 22-23 37.34544422384437 39.0 36.0 41.0 31.5 41.0 24-25 37.18113591643367 39.0 36.0 40.5 31.0 41.0 26-27 36.89488947480055 39.0 36.0 40.0 31.0 41.0 28-29 36.671211126615276 38.5 35.0 40.0 30.0 41.0 30-31 36.40098352395138 38.0 35.0 40.0 30.0 41.0 32-33 36.33581424570269 38.0 35.0 40.0 30.0 41.0 34-35 36.41334126474008 38.0 35.0 40.0 30.0 41.0 36-37 36.37243468563014 38.0 35.0 40.0 30.0 41.0 38-39 36.23073752825866 38.0 35.0 40.0 30.0 41.0 40-41 36.04459242939713 38.0 35.0 40.0 29.5 41.0 42-43 35.829128560832025 38.0 35.0 40.0 29.0 41.0 44-45 35.57416817828922 38.0 34.0 40.0 27.5 41.0 46-47 35.25403388905502 38.0 34.0 40.0 27.0 41.0 48-49 34.914380450745924 38.0 34.0 40.0 26.0 41.0 50-51 34.627609183957254 38.0 33.0 40.0 25.5 41.0 52-53 34.33356965222686 38.0 33.0 40.0 24.0 41.0 54-55 34.01048402643927 37.0 33.0 40.0 23.5 41.0 56-57 32.650250117019105 36.5 31.5 39.5 15.5 41.0 58-59 29.22293799858893 34.5 23.5 38.5 7.0 40.0 60-61 29.19583102078895 34.0 24.0 38.0 8.0 40.0 62-63 31.13754685292057 35.0 29.0 38.0 8.0 40.0 64-65 30.720861236470355 35.0 29.0 38.0 4.0 40.0 66-67 25.283141159050597 28.5 18.0 36.0 2.0 39.0 68-69 17.61591793891824 13.0 7.0 29.5 2.0 35.5 70-71 16.45991446637879 14.5 7.5 26.0 2.0 33.5 72-73 18.617373886004273 19.0 7.0 30.0 2.0 34.5 74-75 19.32644003932969 19.0 7.5 31.0 2.0 35.0 76-77 20.257562171053557 23.0 8.5 31.5 2.0 34.5 78-79 23.331445398261394 28.5 12.0 34.0 2.0 36.0 80-81 26.007971586996412 31.5 22.0 34.5 2.0 36.0 82-83 27.037843556221695 33.0 26.0 35.0 2.0 36.0 84-85 27.046262291030843 33.0 26.0 35.0 2.0 36.0 86-87 26.772573820642613 33.0 25.5 35.0 2.0 35.5 88-89 26.342348198147256 33.0 24.5 35.0 2.0 35.0 90-91 24.7131192604795 32.0 12.5 34.5 2.0 35.0 92-93 22.601665353932987 31.0 2.0 34.0 2.0 35.0 94-95 18.20599830075782 23.0 2.0 33.0 2.0 35.0 96-97 16.893232089838477 15.0 2.0 32.5 2.0 35.0 98-99 15.80661843158578 4.5 2.0 32.0 2.0 34.0 100-101 15.18352902254937 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28796371534547305 1101 2 0.4463477553581825 1101 3 0.33572676305369953 1101 4 0.326943667000144 1101 5 0.3130005223534056 1101 6 0.3887687441673435 1101 7 0.4307219778390561 1101 8 0.4955013811828195 1101 9 0.657274442085594 1101 10-11 0.48835239403359054 1101 12-13 0.4288465789372964 1101 14-15 0.521504093516441 1101 16-17 0.5026551922035978 1101 18-19 0.5021314572817559 1101 20-21 0.6631421074639903 1101 22-23 0.6973010579376435 1101 24-25 0.6480818793075827 1101 26-27 0.5834415560801673 1101 28-29 0.45559094462157645 1101 30-31 0.6451757379624468 1101 32-33 0.5648407789674081 1101 34-35 0.7578933355356199 1101 36-37 0.7703214573708124 1101 38-39 0.6446689462265667 1101 40-41 0.8229288908981047 1101 42-43 0.7313689655548501 1101 44-45 0.5525960807256425 1101 46-47 0.34913000270800154 1101 48-49 0.38568500322777766 1101 50-51 0.21729993562077965 1101 52-53 0.4134966797926367 1101 54-55 0.4644908412194937 1101 56-57 1.1769798164501708 1101 58-59 -8.365359008962432 1101 60-61 -9.831178044476754 1101 62-63 -2.4590950182066393 1101 64-65 0.13301832458040508 1101 66-67 -0.9670953281126842 1101 68-69 -3.4199948620325564 1101 70-71 -3.960812611397813 1101 72-73 -6.6862438110826865 1101 74-75 -7.368460486589685 1101 76-77 -8.618138083272168 1101 78-79 -11.931837842520302 1101 80-81 -7.895197027607956 1101 82-83 -3.9354468700289473 1101 84-85 -2.92793004924566 1101 86-87 -2.951885940117105 1101 88-89 -2.996840479345636 1101 90-91 -8.748568781983113 1101 92-93 -14.845867262554709 1101 94-95 -12.776932012952464 1101 96-97 -12.632625300087504 1101 98-99 -12.09533697427743 1101 100-101 -11.771160479713195 1102 1 0.4465647056420359 1102 2 0.4662088760999268 1102 3 0.4602397870526147 1102 4 0.33495604075431373 1102 5 0.36538873898467017 1102 6 0.44881980146954703 1102 7 0.3563881779899063 1102 8 0.48783995562558147 1102 9 0.5114483906914984 1102 10-11 0.5734711291483592 1102 12-13 0.6433484518402821 1102 14-15 0.6853866419097869 1102 16-17 0.7359069678175061 1102 18-19 0.6419227563136758 1102 20-21 0.7197378108957793 1102 22-23 0.6765961756538772 1102 24-25 0.7359940488608387 1102 26-27 0.7681283984945253 1102 28-29 0.7972857130877458 1102 30-31 0.652139866245335 1102 32-33 0.6659969976137887 1102 34-35 0.807104268138005 1102 36-37 0.8269659822251683 1102 38-39 0.6878851034287194 1102 40-41 0.7082579826639801 1102 42-43 0.7990365185544022 1102 44-45 0.7517146064035884 1102 46-47 0.6674824707370419 1102 48-49 0.6713869066972151 1102 50-51 0.6828397944207367 1102 52-53 0.7016704331128452 1102 54-55 0.5551554069140252 1102 56-57 1.0711852088824259 1102 58-59 -8.699543769036982 1102 60-61 -9.686988218295173 1102 62-63 -2.8968841436239217 1102 64-65 -0.2651140083618877 1102 66-67 -1.4816101493059932 1102 68-69 -3.8131721743637144 1102 70-71 -4.229481498849575 1102 72-73 -6.976267825562131 1102 74-75 -7.883216938446809 1102 76-77 -8.879413001655486 1102 78-79 -12.109299821953039 1102 80-81 -8.136012939712415 1102 82-83 -3.764269410525962 1102 84-85 -2.896223719300579 1102 86-87 -2.708353363492236 1102 88-89 -2.8517948631556003 1102 90-91 -8.486679350476738 1102 92-93 -14.730971351242715 1102 94-95 -12.544245575260629 1102 96-97 -12.279780497021802 1102 98-99 -11.795085225550679 1102 100-101 -11.581136535989437 1103 1 0.28256279914695526 1103 2 0.2882982213921821 1103 3 0.22110081936362747 1103 4 0.1462741985906959 1103 5 0.2774236166299957 1103 6 0.23745627516730394 1103 7 0.2667599700188177 1103 8 0.3646390829198154 1103 9 0.30410175777081605 1103 10-11 0.3183321308738911 1103 12-13 0.3948267501659757 1103 14-15 0.49212080054139307 1103 16-17 0.4542076638921131 1103 18-19 0.4725849574998833 1103 20-21 0.5094549716454324 1103 22-23 0.3952779158658828 1103 24-25 0.5574283550674011 1103 26-27 0.4712070821973029 1103 28-29 0.5539915801479509 1103 30-31 0.5937069389342824 1103 32-33 0.49887473469588883 1103 34-35 0.4657428828195407 1103 36-37 0.3051865698232561 1103 38-39 0.3650043587741649 1103 40-41 0.6217367499670772 1103 42-43 0.7736441960202924 1103 44-45 0.7556429293251128 1103 46-47 0.5572479310612977 1103 48-49 0.5608732744927423 1103 50-51 0.5670539896529689 1103 52-53 0.6041244083335116 1103 54-55 0.48539438148474545 1103 56-57 1.1050669144308856 1103 58-59 -6.446025801340351 1103 60-61 -8.40960210461403 1103 62-63 -2.279663369964137 1103 64-65 -0.10174929942098387 1103 66-67 -1.7868991438847175 1103 68-69 -4.089963782726805 1103 70-71 -4.849476630660261 1103 72-73 -7.458771017921876 1103 74-75 -7.904558596128991 1103 76-77 -8.966723084924462 1103 78-79 -12.082588500481776 1103 80-81 -8.191368840034329 1103 82-83 -4.1238852313765975 1103 84-85 -3.304408012125993 1103 86-87 -2.9671709032469913 1103 88-89 -3.137944354799391 1103 90-91 -8.458344449637423 1103 92-93 -14.436368159647605 1103 94-95 -12.734103684109263 1103 96-97 -12.645217117529878 1103 98-99 -12.18728541772946 1103 100-101 -11.827289774354847 1104 1 0.24125143892211298 1104 2 0.22672241736164978 1104 3 0.18261829020172726 1104 4 0.19641068511018744 1104 5 0.17884662714875788 1104 6 0.11052428171264239 1104 7 0.1952895978323852 1104 8 0.259262190378756 1104 9 0.32854345933407103 1104 10-11 0.3440473465376215 1104 12-13 0.20563957294767476 1104 14-15 0.33402493308643244 1104 16-17 0.3453185683477926 1104 18-19 0.4126086195468588 1104 20-21 0.32200534390701563 1104 22-23 0.3024922100342593 1104 24-25 0.3631161231323716 1104 26-27 0.30490229737257124 1104 28-29 0.15990178460531723 1104 30-31 0.2426395166602049 1104 32-33 0.27171321281013405 1104 34-35 0.1756193216579902 1104 36-37 0.29294106129120934 1104 38-39 0.3124819413564879 1104 40-41 0.3527970144484627 1104 42-43 0.41399235406285584 1104 44-45 0.3021385228677005 1104 46-47 0.4151786620391391 1104 48-49 0.4159373658569834 1104 50-51 0.4881778382534421 1104 52-53 0.4588670095993663 1104 54-55 0.2832160923287077 1104 56-57 0.9800680886094781 1104 58-59 -6.647731835866836 1104 60-61 -8.639063372689336 1104 62-63 -2.3270372490932516 1104 64-65 -0.1650069968661576 1104 66-67 -1.7228890327980295 1104 68-69 -4.0813612806931285 1104 70-71 -4.8912306562141445 1104 72-73 -7.749946877937244 1104 74-75 -8.532615452674424 1104 76-77 -9.260196107936483 1104 78-79 -12.263537171029807 1104 80-81 -8.39612492087042 1104 82-83 -4.499628486425095 1104 84-85 -3.6218953356939174 1104 86-87 -3.3518240602844536 1104 88-89 -3.480997538514192 1104 90-91 -9.201425719704137 1104 92-93 -15.111273332984545 1104 94-95 -12.710057680731387 1104 96-97 -12.546783612672535 1104 98-99 -11.831118302996243 1104 100-101 -11.512482396004447 1105 1 0.023846293830427356 1105 2 0.08987629770000538 1105 3 0.07038504078205676 1105 4 0.06628402514133569 1105 5 0.07540139195493367 1105 6 0.06945465629355141 1105 7 0.06743809318020766 1105 8 0.1442422179085483 1105 9 0.25382273647962705 1105 10-11 0.27147937838152103 1105 12-13 0.2284816930218838 1105 14-15 0.22981807635977702 1105 16-17 0.3367333348602628 1105 18-19 0.23360718575652584 1105 20-21 0.34205294167151834 1105 22-23 0.27999071066647474 1105 24-25 0.27568913374827275 1105 26-27 0.30270306545634895 1105 28-29 0.24357685282495822 1105 30-31 0.2708975059666301 1105 32-33 0.2181238664071543 1105 34-35 0.21819308991621256 1105 36-37 0.08363488975334832 1105 38-39 0.2215104274343389 1105 40-41 0.032146072361619815 1105 42-43 0.1081878588882006 1105 44-45 0.08805872721465136 1105 46-47 0.07697079172713472 1105 48-49 0.09538431243110068 1105 50-51 0.10088127158282845 1105 52-53 0.23706228649535177 1105 54-55 0.04388563803618695 1105 56-57 0.6013866791146327 1105 58-59 -6.58817541952774 1105 60-61 -8.786244624925654 1105 62-63 -2.6605921450543164 1105 64-65 -0.15598213983135523 1105 66-67 -1.7719212243198186 1105 68-69 -4.0650719708352465 1105 70-71 -4.772141221313447 1105 72-73 -6.2598568713031 1105 74-75 -6.644806363258624 1105 76-77 -8.617132068696979 1105 78-79 -11.812352415811944 1105 80-81 -8.105743540280578 1105 82-83 -4.136105335040519 1105 84-85 -3.209494863790983 1105 86-87 -2.9917193719428496 1105 88-89 -3.004865818258658 1105 90-91 -8.242447594556904 1105 92-93 -11.4834049564656 1105 94-95 -11.869001011632625 1105 96-97 -12.171582423619768 1105 98-99 -11.73762508166664 1105 100-101 -11.378726215489479 1106 1 0.03358320731022246 1106 2 0.10019468729561964 1106 3 0.1119800979043113 1106 4 0.09869321497774308 1106 5 0.06979666340968294 1106 6 0.019927312362668204 1106 7 0.03992679772551355 1106 8 0.10656170562743483 1106 9 0.026520481357806602 1106 10-11 0.15424430061983685 1106 12-13 0.10623692399160234 1106 14-15 0.21687924050274177 1106 16-17 0.007302804387343542 1106 18-19 0.19634177730097235 1106 20-21 0.13240268731706095 1106 22-23 0.09611410399967468 1106 24-25 0.1453959811422152 1106 26-27 0.27587065494347485 1106 28-29 0.13893628973910666 1106 30-31 0.1566887170113631 1106 32-33 0.04811784657478313 1106 34-35 0.05482558907277735 1106 36-37 0.13117916198207524 1106 38-39 0.06784378722847606 1106 40-41 0.0074412756798025725 1106 42-43 -0.004404340467473844 1106 44-45 -0.07268303520704222 1106 46-47 -0.20910470755512023 1106 48-49 0.014861943130931365 1106 50-51 0.12097775428076574 1106 52-53 -0.020541555613043272 1106 54-55 -0.2972440397374001 1106 56-57 0.41330313574868427 1106 58-59 -6.5760153581386795 1106 60-61 -8.882472472273289 1106 62-63 -2.6738195690291278 1106 64-65 -0.43944876945289835 1106 66-67 -1.76411198023877 1106 68-69 -4.353193220843515 1106 70-71 -4.886641740606677 1106 72-73 -6.484729694075265 1106 74-75 -6.9894201017068305 1106 76-77 -8.641187748838808 1106 78-79 -11.95288027572846 1106 80-81 -8.352352257791676 1106 82-83 -4.45042145562239 1106 84-85 -3.5764775716571187 1106 86-87 -3.2383464466350773 1106 88-89 -3.44644946416021 1106 90-91 -8.564632330643484 1106 92-93 -11.685967023583423 1106 94-95 -12.035665528359159 1106 96-97 -12.215194620726278 1106 98-99 -11.77423922364207 1106 100-101 -11.604384758690045 1107 1 0.03861917853324215 1107 2 0.10163938519583482 1107 3 -0.004305410274511701 1107 4 0.06907593319341032 1107 5 0.020514138210330657 1107 6 0.12724048621383588 1107 7 0.16219776144234288 1107 8 0.05260779558473416 1107 9 -0.030588953045885603 1107 10-11 0.1592641871496454 1107 12-13 0.077447760200684 1107 14-15 0.09602519203420457 1107 16-17 0.14137749585368198 1107 18-19 0.09679507320083758 1107 20-21 0.13490994979976279 1107 22-23 0.09597714039981753 1107 24-25 0.21954787913601592 1107 26-27 0.26066867018981554 1107 28-29 0.16997682316601725 1107 30-31 0.13501434918855182 1107 32-33 0.18406079952949028 1107 34-35 0.20022780388629258 1107 36-37 0.07408093812213679 1107 38-39 0.009304764787891884 1107 40-41 -0.052195892634323116 1107 42-43 0.14736058905232596 1107 44-45 0.375091428149112 1107 46-47 0.15274776168899962 1107 48-49 0.13140724347601207 1107 50-51 -0.007383459187479957 1107 52-53 0.11355311986608996 1107 54-55 0.12124848331732352 1107 56-57 0.8931207450758549 1107 58-59 -6.643290318658071 1107 60-61 -8.991221083140694 1107 62-63 -2.502066239985048 1107 64-65 -0.11649932413110875 1107 66-67 -1.6325781828864052 1107 68-69 -4.281147930246725 1107 70-71 -4.675525755346108 1107 72-73 -6.086242630067934 1107 74-75 -6.906994822418067 1107 76-77 -9.13067859518612 1107 78-79 -12.336480225037306 1107 80-81 -8.317759444237641 1107 82-83 -4.288898478881833 1107 84-85 -3.335435770951346 1107 86-87 -3.019280236188802 1107 88-89 -3.130967703227494 1107 90-91 -8.6751565941595 1107 92-93 -11.691901787967037 1107 94-95 -12.4722718530555 1107 96-97 -12.839564880615928 1107 98-99 -12.192084459648386 1107 100-101 -11.622587269851847 1108 1 0.02280339079645799 1108 2 0.03417134128234167 1108 3 0.02244291583524216 1108 4 0.056116575908596644 1108 5 -0.03630462696101944 1108 6 0.11068400662686173 1108 7 0.13565477564749528 1108 8 0.06059131702446052 1108 9 -0.018700298831646478 1108 10-11 0.11247134619304688 1108 12-13 0.08951058700346692 1108 14-15 0.07154844315807907 1108 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2.0996844048112138 2312 84-85 1.7309411275298707 2312 86-87 1.6248607010613974 2312 88-89 1.8746802783872134 2312 90-91 3.306958963394454 2312 92-93 5.316294155278367 2312 94-95 9.396317025841384 2312 96-97 9.868227635371284 2312 98-99 10.341563621886388 2312 100-101 9.63973787003624 2313 1 -0.33151606123732336 2313 2 -0.3273515913019658 2313 3 -0.23094792071756842 2313 4 -0.23220279304189972 2313 5 -0.4145484700103026 2313 6 -0.370425330975678 2313 7 -0.4745204501952429 2313 8 -0.4619310435633821 2313 9 -0.5113366432949391 2313 10-11 -0.5526227296492081 2313 12-13 -0.42760337130789594 2313 14-15 -0.5069632635962762 2313 16-17 -0.583735203955122 2313 18-19 -0.5730646503056249 2313 20-21 -0.5523158181752024 2313 22-23 -0.5704019820075814 2313 24-25 -0.6420637719819524 2313 26-27 -0.5450315971824935 2313 28-29 -0.6739117023771826 2313 30-31 -0.6560174022128393 2313 32-33 -0.3608932059181882 2313 34-35 -0.5156302306891831 2313 36-37 -0.6239531111274914 2313 38-39 -0.8779728739161925 2313 40-41 -0.9666486564420822 2313 42-43 -0.7248707794578806 2313 44-45 -0.7260157738635726 2313 46-47 -0.5881259969843171 2313 48-49 -0.8384569905286341 2313 50-51 -0.7140569918347168 2313 52-53 -0.8344896997966842 2313 54-55 -0.7659245368871979 2313 56-57 0.409533946802874 2313 58-59 3.93557574209834 2313 60-61 3.6767354607151326 2313 62-63 1.0008850040354105 2313 64-65 0.7567788558327564 2313 66-67 1.4836579817868696 2313 68-69 -0.08055918494202885 2313 70-71 2.5736899191362497 2313 72-73 7.9714579179575935 2313 74-75 9.419776362514863 2313 76-77 8.445548197674263 2313 78-79 6.007310573045235 2313 80-81 3.0806854362216285 2313 82-83 1.5140353496039687 2313 84-85 1.0373543981672775 2313 86-87 0.8171145977872776 2313 88-89 0.8834740349180414 2313 90-91 2.465537746919363 2313 92-93 4.29741898112302 2313 94-95 8.440156824691961 2313 96-97 9.023537740735268 2313 98-99 9.399655314676354 2313 100-101 8.603237086337183 2314 1 -0.3879057356097739 2314 2 -0.2857153367924674 2314 3 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1.2489052648534553 2314 64-65 1.1360377222998252 2314 66-67 1.6666376025262473 2314 68-69 -0.27697780334026234 2314 70-71 2.5928105308177365 2314 72-73 8.286810785483748 2314 74-75 9.679235949824157 2314 76-77 8.739674861541374 2314 78-79 6.420700136960004 2314 80-81 3.3618173528172477 2314 82-83 1.8641319970500305 2314 84-85 1.4676827623567767 2314 86-87 1.294266009135189 2314 88-89 1.5603445474235293 2314 90-91 2.921612187411281 2314 92-93 5.024943353495338 2314 94-95 8.958582629735481 2314 96-97 9.544828125993025 2314 98-99 9.803829889108803 2314 100-101 9.001696352919959 2315 1 -0.7122899772452165 2315 2 -0.5881467242800262 2315 3 -0.690471886232956 2315 4 -0.44253433226053573 2315 5 -0.670522405562096 2315 6 -0.6963268023570137 2315 7 -0.6419831233928264 2315 8 -0.7065073352648881 2315 9 -1.113817163251369 2315 10-11 -0.9651264172637468 2315 12-13 -0.7608165911237776 2315 14-15 -0.9431859470247943 2315 16-17 -1.056318969715626 2315 18-19 -0.92572867188386 2315 20-21 -1.0762898470046665 2315 22-23 -1.0219584792234997 2315 24-25 -0.967619708791517 2315 26-27 -0.9961494672237379 2315 28-29 -0.9523603761499828 2315 30-31 -1.0637768835001111 2315 32-33 -0.847481023335277 2315 34-35 -0.9918988348664755 2315 36-37 -1.1395315937439747 2315 38-39 -1.010561170306751 2315 40-41 -1.0565781466610744 2315 42-43 -0.8598489461887695 2315 44-45 -0.9126547829585192 2315 46-47 -1.047575932596807 2315 48-49 -0.988575197776079 2315 50-51 -0.8589784849734414 2315 52-53 -0.9547531278031514 2315 54-55 -0.8752319094141896 2315 56-57 0.471788098122218 2315 58-59 3.9152817397419746 2315 60-61 3.5798580790343486 2315 62-63 0.9453405529652912 2315 64-65 0.8454017182655811 2315 66-67 1.7642849854524627 2315 68-69 5.950413260757962 2315 70-71 4.615209775251731 2315 72-73 8.752752486272573 2315 74-75 10.122077137867052 2315 76-77 8.93784575642717 2315 78-79 6.290204187262823 2315 80-81 3.428977066659911 2315 82-83 1.5104684038075504 2315 84-85 0.9599572451352536 2315 86-87 0.8820053240983583 2315 88-89 1.0022266976811096 2315 90-91 2.2732848652128546 2315 92-93 4.29679694486391 2315 94-95 8.385680254971149 2315 96-97 9.088620944793885 2315 98-99 9.396569378174023 2315 100-101 8.514861113630525 2316 1 -0.48769766235774625 2316 2 -0.4334991824328682 2316 3 -0.505021887783748 2316 4 -0.22904416696892582 2316 5 -0.46268979846308866 2316 6 -0.481046510562372 2316 7 -0.5142770105302219 2316 8 -0.48212145752465574 2316 9 -0.630857834006342 2316 10-11 -0.5736251483156991 2316 12-13 -0.646231856899675 2316 14-15 -0.7590372309093993 2316 16-17 -0.823028292525187 2316 18-19 -0.8642773308397693 2316 20-21 -0.7395642390165023 2316 22-23 -0.8063496334168576 2316 24-25 -1.9803688978287965 2316 26-27 -1.301569522240996 2316 28-29 -1.102121485425677 2316 30-31 -0.735955447111948 2316 32-33 -0.695554898199461 2316 34-35 -0.7712452126667984 2316 36-37 -0.7049003433539198 2316 38-39 -1.0835651434684266 2316 40-41 -0.871148741698569 2316 42-43 -0.9995012904621419 2316 44-45 -0.9467124545157688 2316 46-47 -0.7095764165437402 2316 48-49 -0.9373816105878419 2316 50-51 -1.061515552307739 2316 52-53 -0.8679676542979919 2316 54-55 -0.7498009373375751 2316 56-57 0.4990572649135103 2316 58-59 3.9764632935531914 2316 60-61 3.8474769056501223 2316 62-63 1.1233807260861965 2316 64-65 0.811488452255368 2316 66-67 6.005816949997005 2316 68-69 8.769735928423163 2316 70-71 5.608278183424179 2316 72-73 9.228191111843177 2316 74-75 10.255640967567455 2316 76-77 8.811219357561214 2316 78-79 6.141109415409147 2316 80-81 3.1413935453168342 2316 82-83 1.7470904541829881 2316 84-85 1.3082792464002502 2316 86-87 1.1056671649827834 2316 88-89 1.2357644339270166 2316 90-91 2.7389374407862412 2316 92-93 4.6340401525606545 2316 94-95 8.861931255921341 2316 96-97 9.521023075817933 2316 98-99 9.96829542502948 2316 100-101 9.216352496449256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 25.0 4 54.0 5 106.0 6 266.0 7 633.0 8 1471.0 9 2732.0 10 4338.0 11 6524.0 12 9290.0 13 12378.0 14 16203.0 15 22480.0 16 29781.0 17 38565.0 18 41529.0 19 41585.0 20 42928.0 21 43414.0 22 41484.0 23 40037.0 24 41199.0 25 44932.0 26 58915.0 27 85508.0 28 120417.0 29 158846.0 30 188096.0 31 200733.0 32 207160.0 33 215869.0 34 220590.0 35 213998.0 36 180691.0 37 113067.0 38 36864.0 39 1925.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57384171768719 19.325108055895605 14.70345162956382 23.397598596853385 2 15.946337996082935 20.646935376496167 38.9844049983216 24.42232162909929 3 19.344297397386477 24.552865096611406 30.126047426868784 25.976790079133327 4 13.300212465643874 16.952415986721604 36.341888171189595 33.40548337644493 5 13.52057234129442 37.73436423930143 34.21550756868522 14.52955585071893 6 32.538273242658676 36.289409224859675 16.840790233088658 14.33152729939299 7 28.464600664000418 32.920181096876526 20.407045375239925 18.208172863883135 8 26.78561366735409 34.95433536340936 19.295260955729532 18.964790013507006 9 26.538283057042133 16.265474594477986 19.768602013090845 37.427640335389036 10-11 25.24944287636383 26.430862938932325 27.070493597253844 21.249200587449998 12-13 26.127156602755253 23.712739526940958 27.55567513483861 22.60442873546518 14-15 22.756544478911874 25.227131175334783 25.754291630653626 26.26203271509972 16-17 22.286816947505812 28.41340606293797 26.606965122068132 22.69281186748809 18-19 22.29277675346927 26.64443776329659 28.944261920314833 22.118523562919307 20-21 22.933611630189844 25.812724353698346 29.125924632048868 22.127739384062945 22-23 22.550235708475725 25.928012824408555 29.127111928221304 22.394639538894413 24-25 22.49594206316657 26.1671825703312 29.1599537477708 22.17692161873143 26-27 22.187457425813506 26.386165203036988 28.993833496972492 22.432543874177018 28-29 22.143413862753782 26.700424236948038 28.900716962655835 22.25544493764234 30-31 22.26619197314453 26.336997516512973 28.78405738183311 22.612753128509386 32-33 21.99462052266979 26.413692512520985 28.76438645946194 22.827300505347278 34-35 22.637443265675415 26.472174472663077 28.586693992249074 22.303688269412426 36-37 22.78941148598014 26.02172670741691 28.686085531222204 22.502776275380754 38-39 22.26979956526211 26.365792138607276 28.674665376824837 22.68974291930578 40-41 22.560319454682258 26.183754075611628 28.873929491465923 22.38199697824019 42-43 22.348918919190933 26.31999750399813 28.485842737791984 22.845240839018953 44-45 22.35962169240013 26.359327806534534 28.677644444399714 22.603406056665623 46-47 22.703109100845744 26.71350799694434 28.04630185986451 22.53708104234541 48-49 22.39524832100203 26.851064566439685 27.900151454031164 22.853535658527118 50-51 22.41473046330196 26.905301202733682 27.96522372038077 22.714744613583587 52-53 22.793442179477786 26.67896613606922 27.825771561576385 22.701820122876608 54-55 22.299918828146524 26.755112387694325 27.948477509479275 22.996491274679876 56-57 22.294149503179085 26.849290165140726 27.867843077107256 22.988717254572926 58-59 20.74760545836283 26.503466418839412 29.578154384377154 23.170773738420607 60-61 22.02238373202014 26.668843933751063 28.322705699763617 22.986066634465182 62-63 22.02543439610163 26.72601828644334 28.272145422201117 22.976401895253918 64-65 21.245141189393713 26.79657349693504 28.596900235512134 23.361385078159117 66-67 21.214958782359965 26.669452667204414 28.828473850535012 23.287114699900606 68-69 21.962981284337214 25.98197953115856 29.1921507373955 22.862888447108723 70-71 21.123653407631583 26.289949481001447 29.255799444079212 23.330597667287762 72-73 20.90201560910512 26.408237692521453 29.220983352309847 23.468763346063582 74-75 21.39197118100856 26.66518866488421 28.504483555867953 23.43835659823927 76-77 20.04759425410066 26.82570618727338 29.22682328496795 23.89987627365801 78-79 21.141646998159064 26.93860495810057 28.069736940740693 23.85001110299967 80-81 21.89344337511551 26.886255187891006 27.360993499238518 23.859307937754966 82-83 21.98653563183602 26.92300107219097 27.1764390058568 23.914024290116203 84-85 21.86205544796099 26.965539419053737 27.074971947601178 24.097433185384094 86-87 22.02212771917623 26.835303085918888 27.013341511791143 24.129227683113736 88-89 21.869939526365325 26.897254387110547 26.740712373822085 24.49209371270204 90-91 21.43362341767365 26.925266661328966 27.12682186726761 24.514288053729768 92-93 21.306190836434062 26.605661963716877 27.46003823074267 24.628108969106393 94-95 19.88507065857867 26.591048357827667 28.531874358212 24.99200662538166 96-97 19.834822207118886 26.177815699102435 28.794061129392666 25.193300964386005 98-99 19.96427642487889 26.40811441297916 28.09779074682186 25.529818415320086 100-101 19.91393817690351 26.25495714080805 28.26001579820321 25.57108888408523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 385.0 1 292.0 2 218.0 3 243.5 4 262.5 5 323.0 6 447.0 7 698.0 8 1013.0 9 1318.5 10 1480.5 11 1595.0 12 1853.5 13 2096.0 14 2347.5 15 2603.0 16 2852.0 17 3191.0 18 3504.0 19 3879.5 20 4452.0 21 5206.0 22 6186.5 23 7483.0 24 9119.0 25 11183.0 26 14067.5 27 17317.0 28 20418.5 29 23740.0 30 27897.5 31 32941.5 32 38407.5 33 42915.5 34 46766.0 35 51186.5 36 55925.0 37 60787.0 38 64735.5 39 67936.5 40 71652.0 41 75952.5 42 79922.5 43 83411.5 44 86569.5 45 90072.5 46 92918.0 47 95023.0 48 97001.0 49 97028.0 50 95718.5 51 93977.0 52 92855.5 53 92087.5 54 89378.5 55 84143.5 56 77133.5 57 69022.5 58 60232.5 59 50995.5 60 41899.5 61 33524.5 62 26110.5 63 19521.5 64 14081.5 65 10244.0 66 7512.5 67 5567.5 68 3902.5 69 2584.0 70 1746.0 71 1180.5 72 840.0 73 620.0 74 426.5 75 272.0 76 175.5 77 95.0 78 60.5 79 39.5 80 25.5 81 14.5 82 6.5 83 4.0 84 2.5 85 1.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02036514790691759 2 0.0049504213291519045 3 0.0038637434764112423 4 0.0 5 0.06612635970566323 6 1.2074198363785132E-4 7 0.0 8 4.024732787928377E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.81112975456777E-4 16-17 0.0 18-19 0.0013684091478956483 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.408656475774932E-4 28-29 0.002354468680938101 30-31 0.009136143428597417 32-33 3.62225950913554E-4 34-35 0.0012074198363785133 36-37 0.02589915549031911 38-39 0.02461124099818203 40-41 0.02632175243305159 42-43 0.026744349375784068 44-45 0.027348059293973326 46-47 0.02895795240914468 48-49 0.0287768394336879 50-51 0.023645305129079218 52-53 0.02088836316934828 54-55 0.016159302143532436 56-57 0.15348318486764867 58-59 0.7406715749624593 60-61 0.30288126595555004 62-63 3.8234961485319584E-4 64-65 0.1387929101917101 66-67 1.3801009966445803 68-69 2.6517958156463095 70-71 2.1401717836448544 72-73 1.802235095106448 74-75 1.5148892977123016 76-77 1.0946266999968206 78-79 0.4057131886871201 80-81 2.0123663939641885E-4 82-83 2.0123663939641885E-4 84-85 4.024732787928377E-5 86-87 0.0024550870006363103 88-89 2.0123663939641886E-5 90-91 0.21795940413026127 92-93 0.16793197557631157 94-95 1.2493776756926664 96-97 1.224846929350243 98-99 0.9690349133495155 100-101 0.38927215524843267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2484637.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.85921660441123 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90800237436461 35.13368663599598 2 14.59149565268957 14.258580933541692 3 5.4874326459507765 8.043349807518794 4 2.629630670494222 5.139267780771213 5 1.4321567125789394 3.498702751567796 6 0.9081751619899668 2.662363617264843 7 0.5835935438174144 1.9959746356417705 8 0.41974625376368846 1.6406778505224175 9 0.31286705953665334 1.3757795487257913 >10 1.560161354813874 13.806856637284673 >50 0.10512049903471708 3.522243276623797 >100 0.0548351108648248 4.799249265442894 >500 0.004218670306620173 1.4217898168796772 >1k 0.0023161327173580617 1.835163313767103 >5k 2.481570768597923E-4 0.8663141284515129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8754 0.35232510825525015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7599 0.3058394445546774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5082 0.20453692028252016 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2520 0.1014232662557951 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.024732787928377E-5 6 0.0 0.0 0.0 0.0 8.049465575856754E-5 7 0.0 0.0 0.0 0.0 8.049465575856754E-5 8 0.0 0.0 0.0 0.0 1.2074198363785132E-4 9 0.0 0.0 0.0 0.0 1.2074198363785132E-4 10-11 0.0 0.0 0.0 0.0 1.6098931151713509E-4 12-13 0.0 0.0 0.0 0.0 3.219786230342702E-4 14-15 0.0 0.0 0.0 0.0 3.62225950913554E-4 16-17 0.0 0.0 0.0 0.0 4.0247327879283775E-4 18-19 0.0 0.0 0.0 2.0123663939641886E-5 4.0247327879283775E-4 20-21 0.0 0.0 0.0 1.0061831969820944E-4 5.030915984910472E-4 22-23 0.0 0.0 0.0 2.4148396727570264E-4 5.433389263703309E-4 24-25 0.0 0.0 0.0 9.05564877283885E-4 6.037099181892566E-4 26-27 0.0 0.0 0.0 0.002314221353058817 6.037099181892566E-4 28-29 0.0 0.0 0.0 0.008431815190709952 6.037099181892566E-4 30-31 0.0 0.0 0.0 0.027448677613671535 6.238335821288985E-4 32-33 0.0 0.0 0.0 0.06860157037023919 6.439572460685403E-4 34-35 0.0 0.0 0.0 0.13185024613253365 7.24451901827108E-4 36-37 0.0 0.0 0.0 0.23973320851295382 7.24451901827108E-4 38-39 0.0 0.0 0.0 0.4012859826203989 7.24451901827108E-4 40-41 0.0 0.0 0.0 0.575758148977094 7.24451901827108E-4 42-43 0.0 4.024732787928377E-5 0.0 0.7492643794646864 7.646992297063917E-4 44-45 0.0 4.024732787928377E-5 0.0 0.9616696523476065 8.250702215253174E-4 46-47 0.0 4.024732787928377E-5 0.0 1.2075607020260906 8.85441213344243E-4 48-49 0.0 4.024732787928377E-5 0.0 1.4438125166774864 0.0010464305248613782 50-51 0.0 4.024732787928377E-5 0.0 1.6889187434623247 0.001086677852740662 52-53 0.0 4.024732787928377E-5 0.0 1.9175638131445358 0.001247667164257797 54-55 0.0 4.024732787928377E-5 0.0 2.159027656756299 0.0012879144921370807 56-57 0.0 4.024732787928377E-5 0.0 2.3785567066738524 0.0012879144921370807 58-59 0.0 4.024732787928377E-5 0.0 2.533669908320612 0.0012879144921370807 60-61 0.0 4.024732787928377E-5 0.0 2.6507493851214483 0.0012879144921370807 62-63 8.049465575856754E-5 4.024732787928377E-5 0.0 2.7523537643527 0.0013482854839560064 64-65 8.049465575856754E-5 4.024732787928377E-5 0.0 2.857962752707941 0.0013885328118352902 66-67 8.049465575856754E-5 4.024732787928377E-5 0.0 2.9490223320348203 0.0014086564757749321 68-69 8.049465575856754E-5 4.024732787928377E-5 0.0 3.0481716242654358 0.0014086564757749321 70-71 8.049465575856754E-5 4.024732787928377E-5 0.0 3.225581845557319 0.001428780139714574 72-73 8.049465575856754E-5 4.024732787928377E-5 0.0 3.4456743580651823 0.0015696457872920672 74-75 8.049465575856754E-5 4.024732787928377E-5 0.0 3.696032861138267 0.0016501404430506348 76-77 8.049465575856754E-5 4.024732787928377E-5 0.0 4.052342454853566 0.0016501404430506348 78-79 8.049465575856754E-5 4.024732787928377E-5 0.0 4.5641073525025995 0.0016501404430506348 80-81 8.049465575856754E-5 4.024732787928377E-5 0.0 5.136806704560867 0.0017105114348695606 82-83 8.049465575856754E-5 4.024732787928377E-5 0.0 5.675034220290529 0.0019318717382056211 84-85 1.0061831969820944E-4 4.024732787928377E-5 0.0 6.1115567384692415 0.001972119066084905 86-87 1.2074198363785132E-4 4.024732787928377E-5 0.0 6.494328950265169 0.001972119066084905 88-89 1.2074198363785132E-4 4.024732787928377E-5 0.0 6.885492729923929 0.001972119066084905 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6035 0.0 43.382645 1 GTATCAA 9945 0.0 36.24006 1 TATCAAC 12605 0.0 28.43527 2 ATCAACG 12605 0.0 28.357584 3 TCAACGC 12675 0.0 28.200407 4 CAACGCA 12895 0.0 27.682714 5 AACGCAG 13110 0.0 27.320127 6 GTACATG 10380 0.0 25.586529 1 TACATGG 10210 0.0 25.359062 2 ACATGGG 10055 0.0 24.85631 3 ACGCAGA 14775 0.0 24.113827 7 CGCAGAG 14785 0.0 24.033768 8 CATGGGA 5610 0.0 21.686575 4 GCAGAGT 16145 0.0 21.454636 9 GAGTACT 9005 0.0 21.012516 12-13 AGAGTAC 13820 0.0 20.852604 10-11 AGTACTT 9420 0.0 20.186865 12-13 TAAGGTG 1520 0.0 19.855028 5 GTACTTT 9440 0.0 19.794832 14-15 CATGGGG 4720 0.0 19.281883 4 >>END_MODULE