FastQCFastQC Report
Fri 27 May 2016
SRR939872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570973
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82790.32201816199547795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65030.25293925684944957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.1587336778721519No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26590.10342387881941972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61200.040.9736331
GTATCAA102900.037.765341
TATCAAC127100.030.2416292
ATCAACG128600.030.0363963
TCAACGC130150.029.750984
CAACGCA133250.028.989355
AACGCAG137400.028.1838976
GTACATG108050.026.037851
TACATGG108600.025.429452
ACGCAGA153650.024.8663127
CGCAGAG155450.024.4875728
ACATGGG108300.023.9369473
TAAGGTG14050.022.7716835
GCAGAGT163850.022.0834949
GAGTACT86500.020.96866612-13
GTATAGG6000.020.386881
CATGGGA59800.020.2198334
AGAGTAC139450.020.10977410-11
GTACTTT89350.020.06970814-15
AGTACTT92000.020.02195512-13
Produced by FastQC (version 0.11.5)