FastQCFastQC Report
Fri 27 May 2016
SRR939872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570973
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89090.34652250334795426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.3023368973536478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.19867964385468068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69450.041.479711
GTATCAA110350.034.148171
TATCAAC135850.027.4884282
TCAACGC135850.027.4825464
ATCAACG136450.027.3269313
CAACGCA138300.026.892785
AACGCAG143200.026.0056976
GTACATG108250.024.5515771
TACATGG107850.023.757922
ACGCAGA162400.022.8727057
ACATGGG108100.022.8640883
CGCAGAG164300.022.4927088
AGGTAAG11900.022.3293362
TAAGGTG14900.022.2871025
GAGTACT92100.021.78826112-13
GTAAGGT14750.020.5840024
GTACTTT97000.020.51644314-15
GGTAAGG14150.020.4510333
GCAGAGT179100.020.0247949
AGAGTAC147100.019.86597410-11
Produced by FastQC (version 0.11.5)