##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939872_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2570973 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.435434755635317 31.0 31.0 34.0 30.0 34.0 2 31.668520439537872 31.0 31.0 34.0 30.0 34.0 3 31.707763947734964 31.0 31.0 34.0 30.0 34.0 4 35.518408011286 37.0 35.0 37.0 33.0 37.0 5 35.25175682513974 37.0 35.0 37.0 33.0 37.0 6 35.27801147658882 37.0 35.0 37.0 32.0 37.0 7 35.188987593413074 37.0 35.0 37.0 32.0 37.0 8 35.146027982401996 37.0 35.0 37.0 32.0 37.0 9 36.73180115077054 39.0 35.0 39.0 32.0 39.0 10-11 36.59388857837091 39.0 35.0 39.0 32.0 39.0 12-13 36.51692102561948 38.0 35.0 39.0 32.0 39.0 14-15 37.55468260460145 39.0 36.0 41.0 32.0 41.0 16-17 37.47931911381411 39.0 36.0 41.0 32.0 41.0 18-19 37.46985693743186 39.0 36.0 41.0 32.0 41.0 20-21 37.38755910699957 39.0 36.0 41.0 32.0 41.0 22-23 37.267591686104836 39.0 36.0 40.5 31.0 41.0 24-25 37.11211611323806 39.0 36.0 40.0 31.0 41.0 26-27 36.81737264452019 39.0 36.0 40.0 30.5 41.0 28-29 36.59085490201569 38.5 35.0 40.0 30.0 41.0 30-31 36.31193987645922 38.0 35.0 40.0 30.0 41.0 32-33 36.27130953922892 38.0 35.0 40.0 30.0 41.0 34-35 36.121180580270575 38.0 35.0 40.0 30.0 41.0 36-37 36.154756000938164 38.0 35.0 40.0 30.0 41.0 38-39 36.04763974573051 38.0 35.0 40.0 30.0 41.0 40-41 35.95294310753167 38.0 35.0 40.0 29.0 41.0 42-43 35.71434900327619 38.0 34.5 40.0 28.5 41.0 44-45 35.45834787063108 38.0 34.0 40.0 27.0 41.0 46-47 35.159881686816625 38.0 34.0 40.0 26.5 41.0 48-49 34.827472517214304 38.0 33.5 40.0 26.0 41.0 50-51 34.58343708782628 38.0 33.0 40.0 24.5 41.0 52-53 34.322457878787525 38.0 33.0 40.0 24.0 41.0 54-55 34.0485711051808 37.0 33.0 40.0 24.0 41.0 56-57 33.67648668422422 37.0 32.5 40.0 23.0 41.0 58-59 33.30159398795709 36.5 32.0 39.5 22.5 41.0 60-61 32.980842855992655 36.0 31.0 39.0 22.0 41.0 62-63 32.684787821575725 36.0 31.0 39.0 21.0 40.0 64-65 32.24136931815309 35.0 31.0 39.0 19.5 40.0 66-67 31.371396354609715 35.0 29.5 38.0 14.5 40.0 68-69 29.570911674296074 34.0 26.5 37.0 7.5 39.5 70-71 28.92077260243495 33.5 25.5 36.0 7.5 39.0 72-73 29.453053182588846 34.0 27.5 36.0 7.5 39.0 74-75 29.52667258660437 34.0 28.0 36.0 8.0 39.0 76-77 29.039448099999497 32.5 27.5 35.0 8.0 37.0 78-79 29.558195282486437 34.0 29.0 35.0 7.0 37.0 80-81 29.387879997183944 34.0 29.0 35.0 6.5 37.0 82-83 28.956577723686713 33.5 28.5 35.0 2.0 36.5 84-85 28.618192995414574 33.0 28.0 35.0 2.0 36.0 86-87 28.256273597583483 33.0 27.0 35.0 2.0 36.0 88-89 27.810988874640067 33.0 26.5 35.0 2.0 35.0 90-91 27.344458887744057 33.0 26.0 35.0 2.0 35.0 92-93 26.87559165343238 32.5 25.0 35.0 2.0 35.0 94-95 26.094919511017814 32.0 24.0 34.0 2.0 35.0 96-97 25.580641259165304 32.0 23.0 34.0 2.0 35.0 98-99 24.923638249020897 32.0 19.0 34.0 2.0 35.0 100-101 23.450870351419482 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23935424200886501 1101 2 0.28572222764216093 1101 3 0.3277792078620223 1101 4 0.17786649725216108 1101 5 0.26309469394369245 1101 6 0.18771852690603197 1101 7 0.21600127076634124 1101 8 0.21684508817230608 1101 9 0.4222479474848271 1101 10-11 0.2827725231571847 1101 12-13 0.380560542000687 1101 14-15 0.43213854499220616 1101 16-17 0.46738730503909665 1101 18-19 0.26845230200250825 1101 20-21 0.3094686406116125 1101 22-23 0.26308846478313797 1101 24-25 0.19030997145948447 1101 26-27 0.2587283960602207 1101 28-29 0.2865147961409136 1101 30-31 0.2212359195778575 1101 32-33 0.13170488869648267 1101 34-35 0.16165509109936238 1101 36-37 0.18718329165800895 1101 38-39 0.3350609504563664 1101 40-41 0.34487904829993 1101 42-43 0.2511494914790191 1101 44-45 0.1967477555116801 1101 46-47 0.24526628670724193 1101 48-49 0.4240339691242738 1101 50-51 0.34815731684173556 1101 52-53 0.1222715975531159 1101 54-55 -0.11278659185467887 1101 56-57 -0.13371841694358721 1101 58-59 0.07182013132159426 1101 60-61 -0.1556407040917378 1101 62-63 -0.007263975104258691 1101 64-65 -0.26621250733578705 1101 66-67 -0.2759517503560076 1101 68-69 -8.754421486433447 1101 70-71 -6.757085472359286 1101 72-73 -1.85340700730994 1101 74-75 -0.5800982816011562 1101 76-77 -0.48631587664486986 1101 78-79 -0.5761507782391782 1101 80-81 -0.5461131880609216 1101 82-83 -0.5470639735362077 1101 84-85 -0.5040789289635192 1101 86-87 -0.5307280259124596 1101 88-89 -0.29911345394054933 1101 90-91 -0.6235929424863684 1101 92-93 -1.0429187831574254 1101 94-95 -0.8555644536501106 1101 96-97 -1.2766234341722118 1101 98-99 -1.1028924113096394 1101 100-101 -1.0601923310206338 1102 1 0.25148961947752824 1102 2 0.18925783348650072 1102 3 0.27262388644920676 1102 4 0.21100562475326967 1102 5 0.38208523451930176 1102 6 0.29650871972776827 1102 7 0.3633765724557989 1102 8 0.24878491896669175 1102 9 0.4827061910682531 1102 10-11 0.3828925501470408 1102 12-13 0.3672231104485135 1102 14-15 0.5188301469956826 1102 16-17 0.42816443113880354 1102 18-19 0.332943614643618 1102 20-21 0.2825431806162726 1102 22-23 0.3688304586585147 1102 24-25 0.2922840910452962 1102 26-27 0.43332841422647306 1102 28-29 0.3003586118313635 1102 30-31 0.23223590660197146 1102 32-33 0.23996728716014104 1102 34-35 0.019590983710891408 1102 36-37 0.09186061369366172 1102 38-39 0.23073770804077753 1102 40-41 0.20063564005246093 1102 42-43 0.231323790132123 1102 44-45 0.3019932593655241 1102 46-47 0.22288887643552613 1102 48-49 0.17261586645903293 1102 50-51 0.33471413177959164 1102 52-53 0.31874734561321816 1102 54-55 0.10440224628406014 1102 56-57 0.11740253782245702 1102 58-59 -0.1333324327144254 1102 60-61 0.07840203200460394 1102 62-63 0.027045415547910068 1102 64-65 0.05853332740326067 1102 66-67 0.33124046151433717 1102 68-69 -4.755263297348769 1102 70-71 0.26811857352345925 1102 72-73 0.5625189522571823 1102 74-75 0.2903968135121211 1102 76-77 -0.0407187585902129 1102 78-79 -0.07423391477150076 1102 80-81 0.1089138342316609 1102 82-83 -0.002550180164288207 1102 84-85 -0.07359710169857792 1102 86-87 -0.1973279208077443 1102 88-89 -0.40736352530795017 1102 90-91 -0.3325806932454576 1102 92-93 -0.29317894976786363 1102 94-95 -0.14561275640359383 1102 96-97 -0.6941112043951385 1102 98-99 -0.5366728722132805 1102 100-101 -0.5780732707346452 1103 1 0.19437861378492727 1103 2 0.2612012237458323 1103 3 0.21746892123731598 1103 4 0.22820360071784762 1103 5 0.27641635406389753 1103 6 0.26424274882327836 1103 7 0.2095040234361889 1103 8 0.3479895362974972 1103 9 0.40186020751353624 1103 10-11 0.3564947006846708 1103 12-13 0.3500322502334612 1103 14-15 0.45706069728031196 1103 16-17 0.4891163602975652 1103 18-19 0.3783175868131323 1103 20-21 0.35434504652393173 1103 22-23 0.1714957021752781 1103 24-25 0.16904642501113898 1103 26-27 0.35444637754082464 1103 28-29 0.34146313428550457 1103 30-31 0.22065304765193616 1103 32-33 0.3289331315624153 1103 34-35 0.13638810578552096 1103 36-37 0.30055685025280354 1103 38-39 0.4741580116460824 1103 40-41 0.3454877905900915 1103 42-43 0.28969428349076054 1103 44-45 0.31684145670897124 1103 46-47 0.14655664999656892 1103 48-49 0.10273209036794384 1103 50-51 0.10683067194403861 1103 52-53 0.24268788957273557 1103 54-55 0.1787681539374404 1103 56-57 0.32197322093441016 1103 58-59 0.2558603250338365 1103 60-61 0.22257739569846535 1103 62-63 0.06152122199952004 1103 64-65 0.19531305542350452 1103 66-67 0.290679107940651 1103 68-69 -3.71784552378773 1103 70-71 0.763477523555089 1103 72-73 0.8644512735728078 1103 74-75 0.46938005196879473 1103 76-77 0.18432630782344717 1103 78-79 0.1378201649248929 1103 80-81 0.06761955845683332 1103 82-83 -0.07766719471204553 1103 84-85 -0.08029302200218424 1103 86-87 -0.30316362605758584 1103 88-89 -0.33673101423900675 1103 90-91 -0.4243422365724676 1103 92-93 -0.3820499175098 1103 94-95 -0.3306973547590637 1103 96-97 -0.5985411537942475 1103 98-99 -0.2224587672164411 1103 100-101 -0.41038430805280157 1104 1 0.20956320740515366 1104 2 0.23822521255004148 1104 3 0.24465521856462402 1104 4 0.15918125477404033 1104 5 0.21636067935025238 1104 6 0.08922031160944499 1104 7 0.0762838649305877 1104 8 0.11874631689957482 1104 9 0.20178293222794252 1104 10-11 0.2184859083391828 1104 12-13 0.2251473265199806 1104 14-15 0.2595528252408812 1104 16-17 0.25236861542769873 1104 18-19 0.16156466997534125 1104 20-21 0.3184751671524779 1104 22-23 0.33885176174418774 1104 24-25 0.1658455853269274 1104 26-27 0.11645142781539874 1104 28-29 0.08024346255334791 1104 30-31 0.12705716088760965 1104 32-33 0.19078500031612577 1104 34-35 0.16600903095932296 1104 36-37 0.14346866819953874 1104 38-39 0.27399235655549603 1104 40-41 0.16014773369844448 1104 42-43 0.23081279761478868 1104 44-45 0.15370313699008875 1104 46-47 0.19429847752665097 1104 48-49 0.2175789849054368 1104 50-51 0.21518583402438196 1104 52-53 0.09906842471930588 1104 54-55 0.09210057457055854 1104 56-57 0.13032625338090753 1104 58-59 0.08168624267785418 1104 60-61 -0.02493154115789764 1104 62-63 0.16121090392349657 1104 64-65 0.21342224479359118 1104 66-67 0.2815880052466646 1104 68-69 -4.295922160183174 1104 70-71 0.44342169672474085 1104 72-73 0.6667852001612857 1104 74-75 0.37832162394281355 1104 76-77 0.46510392841396353 1104 78-79 0.07038713430380383 1104 80-81 -0.08787459265589703 1104 82-83 0.021899881148556233 1104 84-85 0.04533058042559546 1104 86-87 0.0026590971356483806 1104 88-89 -0.04217420625592183 1104 90-91 -0.028968866122657033 1104 92-93 -0.04095604629180016 1104 94-95 0.3016905732593109 1104 96-97 0.02671310927637549 1104 98-99 0.17777412345728294 1104 100-101 0.3364145480295839 1105 1 0.15452874447905884 1105 2 0.20508287667285785 1105 3 0.1020812697847866 1105 4 0.1552560198624846 1105 5 0.2209886379717645 1105 6 0.15976861583548185 1105 7 0.2015602032951449 1105 8 0.19817280595933084 1105 9 0.22667526228592294 1105 10-11 0.24639089007573745 1105 12-13 0.15900047405926188 1105 14-15 0.2665928793137766 1105 16-17 0.3012960852069 1105 18-19 0.275006743296089 1105 20-21 0.31668130651216586 1105 22-23 0.26294279525482267 1105 24-25 0.2869877131861145 1105 26-27 0.31856618421776517 1105 28-29 0.25721352711259016 1105 30-31 0.353647916697156 1105 32-33 0.33792466437 1105 34-35 0.4283908164786183 1105 36-37 0.32829833651688745 1105 38-39 0.44925776366234516 1105 40-41 0.32721079350185533 1105 42-43 0.4496339631225794 1105 44-45 0.4463805644143477 1105 46-47 0.2772265784312893 1105 48-49 0.2588854381483827 1105 50-51 0.22503081641504252 1105 52-53 0.10931434266061757 1105 54-55 0.41099092226999545 1105 56-57 0.16751241800328387 1105 58-59 0.0319681718945688 1105 60-61 0.18933208542091506 1105 62-63 0.17802331087153078 1105 64-65 0.13283537768321452 1105 66-67 0.7832017653135104 1105 68-69 2.0376971733518943 1105 70-71 2.147150065872431 1105 72-73 1.2630338193902197 1105 74-75 0.6238394399777007 1105 76-77 0.3585451590555486 1105 78-79 0.3913886732245295 1105 80-81 0.3199362329428084 1105 82-83 0.3290628455760043 1105 84-85 0.054570586943103905 1105 86-87 0.04137968917963519 1105 88-89 0.38680539766719235 1105 90-91 0.34425579891974323 1105 92-93 0.2770803521445906 1105 94-95 0.5806622365518237 1105 96-97 0.11325765916047814 1105 98-99 0.5770357332043119 1105 100-101 0.5289439762423065 1106 1 0.4103141094785343 1106 2 0.3570487590216267 1106 3 0.3710285693956479 1106 4 0.28059410832275944 1106 5 0.4113348074265488 1106 6 0.3671335313044892 1106 7 0.32268342603835265 1106 8 0.33202655297332484 1106 9 0.48346365262990076 1106 10-11 0.5141193112765734 1106 12-13 0.4889652720403106 1106 14-15 0.7189632994426702 1106 16-17 0.560739046755323 1106 18-19 0.6243094321494027 1106 20-21 0.5983160928864066 1106 22-23 0.663679594423769 1106 24-25 0.6340757451504047 1106 26-27 0.726234906036332 1106 28-29 0.6132750272670009 1106 30-31 0.6993020306096014 1106 32-33 0.8468371997317448 1106 34-35 0.762716674596227 1106 36-37 0.7202063829630845 1106 38-39 0.795093049212511 1106 40-41 0.6331279775059784 1106 42-43 0.5125562039598748 1106 44-45 0.7365500878249733 1106 46-47 0.6312781086734987 1106 48-49 0.706250048953251 1106 50-51 0.7526099462705957 1106 52-53 0.7353097514015801 1106 54-55 0.794175887124176 1106 56-57 0.7662713701006254 1106 58-59 0.895082425088809 1106 60-61 0.9172480022588445 1106 62-63 0.8841458325914218 1106 64-65 0.8514982304280849 1106 66-67 1.2460103338889716 1106 68-69 2.8419693928331213 1106 70-71 3.1646113883159437 1106 72-73 1.9561916987148287 1106 74-75 1.5755427787417453 1106 76-77 1.0751697199546548 1106 78-79 0.8854397361178563 1106 80-81 0.753387379603982 1106 82-83 0.9390260433308377 1106 84-85 1.0657689167920275 1106 86-87 1.1350789980948797 1106 88-89 1.1360328670144284 1106 90-91 1.0673954398064964 1106 92-93 1.1489236027394227 1106 94-95 1.5682558246435363 1106 96-97 1.3663745508053466 1106 98-99 1.627026755964792 1106 100-101 1.6185633738236866 1107 1 0.07023478812990547 1107 2 -0.0157147735393508 1107 3 0.13260253649399445 1107 4 0.06080930332601042 1107 5 0.07945038623895329 1107 6 0.03871675295516752 1107 7 0.016167557621258766 1107 8 0.011181236712921816 1107 9 0.02083349937878154 1107 10-11 0.16418710590846075 1107 12-13 0.05809868867646628 1107 14-15 0.11019675885594893 1107 16-17 0.06837627422890336 1107 18-19 0.08272267634990271 1107 20-21 0.06158257067014006 1107 22-23 0.10001374004847463 1107 24-25 0.21039988080958238 1107 26-27 0.1168081194989199 1107 28-29 0.0751815391065449 1107 30-31 0.1766666664885932 1107 32-33 0.1741686270538807 1107 34-35 0.2270548022853447 1107 36-37 0.00154455153339228 1107 38-39 0.028746425939147002 1107 40-41 -0.15125495062964234 1107 42-43 -0.16174598679765495 1107 44-45 -0.12335275715001615 1107 46-47 -0.06841922243685161 1107 48-49 -0.07838038809624237 1107 50-51 -0.010122691371620363 1107 52-53 0.007103644463512637 1107 54-55 -0.13281002012411847 1107 56-57 -0.19293202149729183 1107 58-59 0.007556489235540198 1107 60-61 0.14622211261908546 1107 62-63 0.17192441713074658 1107 64-65 -2.9155081610099387E-4 1107 66-67 0.5062755846446798 1107 68-69 1.893641137061472 1107 70-71 2.0458267355868003 1107 72-73 1.0070290075952855 1107 74-75 0.3466875348706644 1107 76-77 0.02398195859649732 1107 78-79 0.05768994318994558 1107 80-81 0.1928063513177598 1107 82-83 0.2830760238382126 1107 84-85 0.20087382739257365 1107 86-87 0.16525819988085289 1107 88-89 0.26633001694410297 1107 90-91 0.3464156748081386 1107 92-93 0.2865959554456978 1107 94-95 0.5333178899847049 1107 96-97 0.28923575384974853 1107 98-99 0.5075680080429201 1107 100-101 0.690713999661309 1108 1 -0.19896512042149084 1108 2 -0.09927003834884474 1108 3 -0.13760663158373632 1108 4 -0.07287076552472627 1108 5 -0.09162073750041344 1108 6 0.018826823768158363 1108 7 -0.03774875440262804 1108 8 -0.04291453395966727 1108 9 -0.1350214349454646 1108 10-11 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68-69 1.4470835105875253 2316 70-71 1.6465462740678447 2316 72-73 0.6020429545966799 2316 74-75 0.0898091273646422 2316 76-77 -0.2476992890261691 2316 78-79 -0.2859926991328656 2316 80-81 -0.4208458611838246 2316 82-83 -0.4211683022680397 2316 84-85 -0.5481435429637074 2316 86-87 -0.3531898792366803 2316 88-89 -0.27559023156610607 2316 90-91 -0.389827510179817 2316 92-93 -0.48105968041077674 2316 94-95 -0.20498564748201176 2316 96-97 -0.1670689247352648 2316 98-99 -0.4802600634671599 2316 100-101 -0.5156382255193677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 26.0 4 54.0 5 104.0 6 237.0 7 685.0 8 1577.0 9 2730.0 10 4450.0 11 6668.0 12 9324.0 13 11956.0 14 14669.0 15 18111.0 16 22131.0 17 26079.0 18 23948.0 19 20509.0 20 19895.0 21 20342.0 22 22021.0 23 25075.0 24 29651.0 25 36502.0 26 45339.0 27 55696.0 28 69067.0 29 83717.0 30 99103.0 31 118730.0 32 142462.0 33 173017.0 34 216149.0 35 275888.0 36 347069.0 37 383513.0 38 224027.0 39 20447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.624156214706524 19.33702431245431 14.6769913449639 23.36182812787527 2 15.967876410863537 20.61705863426503 39.0012072412689 24.413857713602532 3 19.41877968237669 24.49351801071191 30.04834751086166 26.03935479604974 4 13.308852329448811 16.957082007473435 36.41737972355214 33.31668593952562 5 13.626202997853342 37.68966068488467 34.133769588400966 14.55036672886102 6 32.52914949254581 36.231836142842525 16.871135458159582 14.367878906452075 7 28.496293037694286 32.93290127900993 20.39309631022963 18.17770937306615 8 26.808566250987464 34.98434250379137 19.301602933986473 18.9054883112347 9 26.58627686871857 16.257735884429746 19.718137841198644 37.43784940565303 10-11 25.23462906844996 26.43596412720009 27.06675643812673 21.262650366223216 12-13 26.176218108863843 23.724578204438554 27.513455022670406 22.5857486640272 14-15 22.797516737826495 25.187604070521157 25.73412867424123 26.28075051741111 16-17 22.289572648708297 28.390532035739895 26.61411699772595 22.70577831782586 18-19 22.305096163981496 26.651271716972524 28.91920685281409 22.12442526623189 20-21 22.92456980295009 25.84552618794519 29.096357682480523 22.133546326624202 22-23 22.562858497541594 25.904686669210452 29.120239691354204 22.41221514189375 24-25 22.505405549425138 26.205955808879843 29.114431173615863 22.174207468079157 26-27 22.147835958061656 26.39876980020533 28.993263034164805 22.46013120756821 28-29 22.177963111991538 26.698725889945862 28.895736280183637 22.227574717878966 30-31 22.270176583638115 26.31976811801648 28.779974663190405 22.630080635154997 32-33 22.010954863116723 26.372654383870632 28.822912327254947 22.793478425757698 34-35 22.651523667678127 26.471827230403417 28.590916145474544 22.28573295644391 36-37 22.829773287891108 26.023214158132944 28.662669739149766 22.484342814826178 38-39 22.31062970721193 26.36147689099717 28.680348446828102 22.647544954962797 40-41 22.576918052777902 26.19869477350682 28.849504149816624 22.374883023898654 42-43 22.33971640980955 26.319833636640432 28.488654601351683 22.851795352198334 44-45 22.360134186929255 26.37822797022316 28.6830693245277 22.578568518319887 46-47 22.702222699770076 26.664319155612343 28.07909360379152 22.554364540826057 48-49 22.351128829848925 26.804684396175354 27.938565525816912 22.905621248158813 50-51 22.466691011244702 26.867771567920595 27.923003913300082 22.74253350753462 52-53 22.803061382565392 26.63985122020142 27.8234454293728 22.73364196786039 54-55 22.29394974636556 26.767054065351054 27.931234982171254 23.007761206112136 56-57 22.31176347568953 26.974140488592536 27.69912115596928 23.01497487974865 58-59 22.36914840603547 26.592554353978425 27.769340501041974 23.26895673894413 60-61 22.0404034352995 26.75575188604602 28.111479965550533 23.092364713103944 62-63 22.115014911933535 26.79534914928562 28.111099982399345 22.978535956381503 64-65 21.844243555690745 26.85324154021223 27.911061443213313 23.39145346088371 66-67 21.655714310196288 26.935669316731865 27.904205050547542 23.50441132252431 68-69 21.798568075754417 26.50688150281174 28.4228235549996 23.271726866434243 70-71 21.722306603533266 26.569630653208947 28.03844456343066 23.669618179827125 72-73 21.761936096413667 26.62763711477408 27.857410842330037 23.75301594648222 74-75 21.909873231753767 27.028206343314647 27.261398131353097 23.80052229357848 76-77 21.904840277307635 26.806456608234097 27.47034988728249 23.818353227175777 78-79 22.002473125473582 26.88240323381188 27.307498416857396 23.807625223857144 80-81 22.12799582097645 26.904350575889595 27.13520039689833 23.83245320623563 82-83 22.007156441507206 26.929149906620864 27.18514273112041 23.878550920751522 84-85 21.8405406225374 26.9308531203495 27.104465176848198 24.124141080264895 86-87 22.028836856161117 26.7914028532688 27.037382348754118 24.14237794181597 88-89 21.912479217446045 26.85079716130101 26.776467137942095 24.46025648331085 90-91 21.7649886793128 26.858033798309343 26.92759933363335 24.449378188744504 92-93 21.842517005148952 26.523462000359793 27.084371794061447 24.54964920042981 94-95 21.43434359162002 26.250321139129145 27.480478633543274 24.83485663570756 96-97 21.77541051016506 25.83563798934146 27.453948051776273 24.935003448717204 98-99 21.530303519541313 26.161428404144445 27.055328641337773 25.25293943497647 100-101 21.371745660962326 25.920774574265202 27.398230639290233 25.309249125482236 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 656.0 1 445.0 2 320.5 3 410.0 4 373.0 5 394.0 6 681.0 7 1347.0 8 2101.0 9 2492.5 10 2334.5 11 2020.5 12 2048.0 13 2262.5 14 2458.0 15 2643.0 16 2824.5 17 3081.5 18 3410.5 19 3801.5 20 4404.5 21 5245.5 22 6242.5 23 7536.0 24 9115.5 25 11182.5 26 14217.0 27 18012.5 28 21123.0 29 23936.0 30 27968.5 31 33642.0 32 40727.0 33 44563.5 34 46369.5 35 49657.5 36 54709.0 37 60538.0 38 64564.5 39 67098.0 40 69827.5 41 74275.5 42 77855.0 43 80553.5 44 84019.5 45 88500.5 46 91989.5 47 94464.0 48 97549.0 49 96899.5 50 94221.0 51 93269.5 52 92949.0 53 94030.0 54 94945.5 55 90504.5 56 83031.5 57 75956.5 58 69405.0 59 60950.5 60 50471.0 61 41864.5 62 34331.5 63 25672.0 64 17961.5 65 13272.5 66 9852.5 67 7702.5 68 5669.5 69 3743.0 70 2671.5 71 1768.0 72 1177.5 73 958.5 74 733.5 75 490.0 76 317.5 77 197.5 78 124.0 79 65.5 80 39.0 81 25.5 82 19.5 83 11.0 84 4.0 85 2.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021859428317605824 2 0.02528225695096759 3 1.1668733977369657E-4 4 0.0 5 0.0 6 7.779155984913104E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.30614129358807E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.555831196982621E-4 26-27 3.30614129358807E-4 28-29 7.779155984913104E-5 30-31 3.889577992456552E-4 32-33 0.0023337467954739313 34-35 0.004045161112154814 36-37 0.10048724743511503 38-39 0.09235802943088084 40-41 0.10138185037338004 42-43 0.1018680476224371 44-45 0.09593644118394087 46-47 0.08926581492687788 48-49 0.0901409699751806 50-51 0.08934360648672701 52-53 0.0702846743236899 54-55 0.05939385594481156 56-57 0.0324974241269745 58-59 0.014391438572089243 60-61 0.009762840761065946 62-63 0.0017697579865677312 64-65 0.00464804570098558 66-67 0.1357073761568091 68-69 0.3894828922746369 70-71 0.37022948121197696 72-73 0.16336227568317518 74-75 0.029988646321840017 76-77 0.03232239311731395 78-79 0.005328721849665477 80-81 0.001458591747171207 82-83 9.72394498114138E-5 84-85 4.278535791702208E-4 86-87 0.0010501860579632692 88-89 2.528225695096759E-4 90-91 6.223324787930484E-4 92-93 0.002353194685436214 94-95 0.07751928938965909 96-97 0.017580892525903617 98-99 0.0192923068425845 100-101 0.010521308469594974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2570973.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.064349785069375 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39529515228458 35.52028084145028 2 14.280574623400252 14.013342169085908 3 5.36316231947526 7.894202159905142 4 2.590271560385184 5.083599595082244 5 1.4133746345088027 3.4673153722442245 6 0.9098342482263159 2.6784255480846957 7 0.5938598240338967 2.03961422907892 8 0.4250121394879424 1.668235541978968 9 0.2937406096997605 1.297097281834973 >10 1.565292009287138 13.821244977719468 >50 0.10723807996158836 3.5948025883174646 >100 0.055613950979833036 4.82645582060857 >500 0.003959322511556755 1.3060206333920383 >1k 0.002533966407388172 1.9427712554506458 >5k 2.3755935069264114E-4 0.8465919857665303 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8909 0.34652250334795426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7773 0.3023368973536478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5108 0.19867964385468068 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.889577992456552E-5 2 0.0 0.0 0.0 0.0 3.889577992456552E-5 3 0.0 0.0 0.0 0.0 3.889577992456552E-5 4 0.0 0.0 0.0 0.0 3.889577992456552E-5 5 0.0 0.0 0.0 0.0 7.779155984913104E-5 6 0.0 0.0 0.0 0.0 1.1668733977369657E-4 7 0.0 0.0 0.0 0.0 1.1668733977369657E-4 8 0.0 0.0 0.0 0.0 1.1668733977369657E-4 9 0.0 0.0 0.0 0.0 1.1668733977369657E-4 10-11 0.0 0.0 0.0 1.944788996228276E-5 1.1668733977369657E-4 12-13 0.0 0.0 0.0 7.779155984913104E-5 1.944788996228276E-4 14-15 0.0 0.0 0.0 9.72394498114138E-5 2.7227045947195866E-4 16-17 0.0 0.0 0.0 1.1668733977369657E-4 3.500620193210897E-4 18-19 0.0 0.0 0.0 1.3613522973597933E-4 3.6950990928337246E-4 20-21 0.0 0.0 0.0 3.111662393965242E-4 3.889577992456552E-4 22-23 0.0 0.0 0.0 6.612282587176139E-4 4.6674935909478627E-4 24-25 0.0 0.0 0.0 0.001380800187322076 5.056451390193518E-4 26-27 0.0 0.0 0.0 0.0027810482646064352 6.223324787930484E-4 28-29 0.0 0.0 0.0 0.00838204057374387 6.417803687553312E-4 30-31 0.0 0.0 0.0 0.029055147603650446 6.806761486798966E-4 32-33 0.0 0.0 0.0 0.07185995341063481 7.390198185667449E-4 34-35 0.0 0.0 0.0 0.13767161304299969 9.334987181895725E-4 36-37 0.0 0.0 0.0 0.24521844453442332 9.334987181895725E-4 38-39 0.0 0.0 0.0 0.40918360480642935 9.334987181895725E-4 40-41 0.0 0.0 0.0 0.5902823561352064 9.334987181895725E-4 42-43 0.0 0.0 0.0 0.7673164984618663 9.529466081518554E-4 44-45 0.0 0.0 0.0 0.9785011355622949 9.918423880764208E-4 46-47 0.0 0.0 0.0 1.2324516826897831 0.0010307381680009864 48-49 0.0 0.0 0.0 1.4952899155300348 0.001147425507774683 50-51 0.0 0.0 0.0 1.7828658644023099 0.0011668733977369657 52-53 0.0 0.0 0.0 2.057158904430346 0.0013613522973597932 54-55 0.0 0.0 0.0 2.3420510444878264 0.0014780396371334898 56-57 0.0 0.0 0.0 2.66692804630776 0.0014780396371334898 58-59 0.0 0.0 0.0 3.007343912207557 0.0014780396371334898 60-61 0.0 0.0 0.0 3.3571142131792127 0.0014780396371334898 62-63 0.0 0.0 0.0 3.690314911903003 0.0015752790869449037 64-65 0.0 0.0 0.0 4.02808586476793 0.0016530706467940348 66-67 0.0 0.0 0.0 4.398101419190322 0.0016725185367563176 68-69 0.0 0.0 0.0 4.815919887140005 0.0016725185367563176 70-71 0.0 0.0 0.0 5.281230102377583 0.0017697579865677314 72-73 0.0 0.0 0.0 5.749068543310257 0.0019058932163037107 74-75 0.0 0.0 0.0 6.212978510470549 0.0019836847761528415 76-77 0.0 0.0 0.0 6.711175107634347 0.0020031326661151243 78-79 0.0 0.0 0.0 7.273258023324244 0.002022580556077407 80-81 0.0 0.0 0.0 7.872291929942477 0.002022580556077407 82-83 0.0 0.0 0.0 8.474340259504864 0.0021781636757756693 84-85 0.0 0.0 0.0 9.051184123676133 0.002217059455700235 86-87 0.0 0.0 0.0 9.635729352272467 0.002294851015549366 88-89 0.0 0.0 0.0 10.253277650134793 0.00239209046536078 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6945 0.0 41.47971 1 GTATCAA 11035 0.0 34.14817 1 TATCAAC 13585 0.0 27.488428 2 TCAACGC 13585 0.0 27.482546 4 ATCAACG 13645 0.0 27.326931 3 CAACGCA 13830 0.0 26.89278 5 AACGCAG 14320 0.0 26.005697 6 GTACATG 10825 0.0 24.551577 1 TACATGG 10785 0.0 23.75792 2 ACGCAGA 16240 0.0 22.872705 7 ACATGGG 10810 0.0 22.864088 3 CGCAGAG 16430 0.0 22.492708 8 AGGTAAG 1190 0.0 22.329336 2 TAAGGTG 1490 0.0 22.287102 5 GAGTACT 9210 0.0 21.788261 12-13 GTAAGGT 1475 0.0 20.584002 4 GTACTTT 9700 0.0 20.516443 14-15 GGTAAGG 1415 0.0 20.451033 3 GCAGAGT 17910 0.0 20.024794 9 AGAGTAC 14710 0.0 19.865974 10-11 >>END_MODULE