FastQCFastQC Report
Fri 27 May 2016
SRR939871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400592
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156301.1159566811748174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112870.8058735163416613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.580397431943064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49630.3543501605035585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.17906713732478838No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.13944103636176702No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.1369420930577927No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.13480014165438614No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.111238676216914No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15070.10759735883112283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72150.043.9796751
ATGCGAT250.00156915138.2292590-91
GTATCAA123450.036.884371
CATTCCG2950.033.7704779
CATGGGT10150.032.7168854
TCGCCGA452.3912937E-531.71084694-95
TCGACGC600.003975977731.6263184
CCGCAGA1805.456968E-1231.6206631
GTACATG101800.029.8656851
GCGCAGA1751.546141E-1029.8137661
TATCAAC156800.029.704872
TCAACGC158750.029.3153554
ATCAACG158750.029.2257063
CAACGCA159200.029.1430975
ACATGGG101600.028.9025963
AACGCAG161600.028.7396376
TACATGG109800.028.3376432
CATGGGG32450.027.9227774
CGCATAT350.00815088827.30660890-91
ATGGGTA8550.025.5229935