FastQCFastQC Report
Fri 27 May 2016
SRR939871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400592
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162201.1580817254418132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127910.9132566800324433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97770.6980619623701978No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56740.40511440876429394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30540.21805065286678776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.1568622411094737No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.14986519985834562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.13772747523904177No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.12844568582428No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT16170.11545118064361355No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT15590.11131007459702755No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14670.10474142362658077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.10209968356237933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68500.048.8188061
GTATCAA116200.036.8968471
CATTCCG4200.034.7808849
CGTCCCG300.003744123332.01795290-91
CGGAGTC504.142437E-529.25903166-67
CATGGGT11900.028.9256614
GTACATG104400.028.4172251
ATCAACG150900.028.155193
TCAACGC152100.027.932064
TATCAAC152300.027.8973732
CAACGCA153900.027.6053715
GGCATTC5500.027.416727
AACGCAG155200.027.3565466
TACATGG108550.027.064292
ACATGGG103050.026.5401213
TTAGGCA6600.025.7197024
CATGGGA52800.025.3624844
ATGGGAT24450.025.2638665
CGGACAT500.001419478124.38252666-67
CCTTTAG6400.024.3201071
Produced by FastQC (version 0.11.5)