##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939871_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1400592 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404443263991226 31.0 31.0 34.0 30.0 34.0 2 31.595268286553114 31.0 31.0 34.0 30.0 34.0 3 31.619867884437436 31.0 31.0 34.0 30.0 34.0 4 35.46152626889201 37.0 35.0 37.0 33.0 37.0 5 35.22770371385814 37.0 35.0 37.0 33.0 37.0 6 35.27010863977518 37.0 35.0 37.0 32.0 37.0 7 35.1942942698516 37.0 35.0 37.0 32.0 37.0 8 35.14582762146292 37.0 35.0 37.0 32.0 37.0 9 36.69119986405748 39.0 35.0 39.0 32.0 39.0 10-11 36.555616839165154 38.5 35.0 39.0 32.0 39.0 12-13 36.520492049076395 38.0 35.0 39.0 32.0 39.0 14-15 37.431519671681684 39.0 36.0 41.0 32.0 41.0 16-17 37.392336597667274 39.0 36.0 41.0 32.0 41.0 18-19 37.51889236836995 39.0 36.0 41.0 32.0 41.0 20-21 37.519597070381664 39.0 36.0 41.0 32.0 41.0 22-23 37.353152809669055 39.0 36.0 41.0 32.0 41.0 24-25 37.19857781566652 39.0 36.0 40.5 31.0 41.0 26-27 36.89674401967169 39.0 35.5 40.0 31.0 41.0 28-29 36.66261659355473 38.5 35.0 40.0 30.0 41.0 30-31 36.32056016313102 38.0 35.0 40.0 30.0 41.0 32-33 36.087941384785864 38.0 35.0 40.0 30.0 41.0 34-35 35.97857049019272 38.0 35.0 40.0 30.0 41.0 36-37 35.82626739264539 38.0 35.0 40.0 30.0 41.0 38-39 35.625194917577716 38.0 35.0 40.0 29.0 41.0 40-41 35.39431076287741 38.0 34.5 40.0 27.5 41.0 42-43 35.07776533066018 38.0 34.0 40.0 26.5 41.0 44-45 34.6376375132801 38.0 34.0 40.0 24.5 41.0 46-47 34.09967963546843 38.0 33.0 40.0 22.5 41.0 48-49 33.635329917634834 38.0 33.0 40.0 19.0 41.0 50-51 33.34065809314918 38.0 33.0 40.0 15.0 41.0 52-53 33.08930545083793 38.0 33.0 40.0 11.0 41.0 54-55 32.81508212241681 38.0 32.5 40.0 8.5 41.0 56-57 31.525785167986108 36.5 30.0 40.0 2.0 41.0 58-59 28.400914042062215 34.5 21.5 39.0 2.0 40.0 60-61 28.362339639238265 34.0 19.5 39.0 2.0 40.0 62-63 30.247523547185764 35.0 28.5 39.0 2.0 40.0 64-65 29.904747064098608 35.0 28.0 39.0 2.0 40.0 66-67 24.644908724310863 28.5 16.5 36.0 2.0 39.0 68-69 17.231211873265018 13.0 7.0 29.5 2.0 36.5 70-71 16.17721327838514 13.0 6.5 26.0 2.0 33.5 72-73 18.245666475319005 16.5 7.0 30.0 2.0 35.0 74-75 18.872136567965548 17.5 7.5 31.0 2.0 35.5 76-77 19.72402526931469 22.5 7.5 31.5 2.0 35.5 78-79 22.590030501387986 27.5 8.0 34.0 2.0 36.0 80-81 24.990171298993566 31.5 16.5 35.0 2.0 37.0 82-83 25.872028756411574 32.5 21.0 35.0 2.0 37.0 84-85 25.781808335332485 33.0 20.0 35.0 2.0 36.0 86-87 25.463505431988757 33.0 18.5 35.0 2.0 36.0 88-89 24.97782830403144 32.5 11.5 35.0 2.0 35.5 90-91 23.33520468487611 31.5 2.0 34.5 2.0 35.0 92-93 21.283087794304123 30.0 2.0 34.0 2.0 35.0 94-95 17.164284459714178 18.5 2.0 33.0 2.0 35.0 96-97 15.955132186960942 10.0 2.0 32.5 2.0 34.5 98-99 14.945630847527331 2.0 2.0 31.5 2.0 34.0 100-101 14.347556604635754 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37725740873424485 1101 2 0.1969795290869918 1101 3 0.3608368322142397 1101 4 0.23283267471032332 1101 5 0.1945975241898097 1101 6 0.4079958299252482 1101 7 0.328097933402816 1101 8 0.4628045474932492 1101 9 0.6292629359683701 1101 10-11 0.43402788816175786 1101 12-13 0.42655266451951235 1101 14-15 0.3779415578606873 1101 16-17 0.29119055271839756 1101 18-19 0.24053679553257012 1101 20-21 0.35301136473468375 1101 22-23 0.592047632565702 1101 24-25 0.526167335722036 1101 26-27 0.3516712169509546 1101 28-29 0.5577779538423329 1101 30-31 0.37155844925868564 1101 32-33 0.5088638201660771 1101 34-35 0.6789350549674467 1101 36-37 0.5904812832336788 1101 38-39 0.6337201529704046 1101 40-41 0.4301575853865174 1101 42-43 0.8906582105314982 1101 44-45 1.00338811154122 1101 46-47 0.7884084506416471 1101 48-49 0.8383092880019376 1101 50-51 0.85434122480045 1101 52-53 1.0101426178640125 1101 54-55 0.8225619218402045 1101 56-57 1.1281404316841694 1101 58-59 -7.649032099366021 1101 60-61 -9.793136309667602 1101 62-63 -2.7790042567508166 1101 64-65 -0.5886203216258714 1101 66-67 -1.9581862587146688 1101 68-69 -4.237725620502889 1101 70-71 -4.513756417108926 1101 72-73 -6.809816332027102 1101 74-75 -7.797673704346291 1101 76-77 -8.11191245643437 1101 78-79 -11.167527069883473 1101 80-81 -7.110934212206548 1101 82-83 -3.2642529434758636 1101 84-85 -2.373154879094745 1101 86-87 -2.3007270981104213 1101 88-89 -2.486892421919734 1101 90-91 -8.284263611981938 1101 92-93 -13.620682688054128 1101 94-95 -11.647624542378846 1101 96-97 -11.810278465072997 1101 98-99 -11.378250430932917 1101 100-101 -11.06397361825649 1102 1 0.5234496510574012 1102 2 0.5200372447006139 1102 3 0.5776660214060669 1102 4 0.48665920843283317 1102 5 0.36539370167952967 1102 6 0.47027499802450023 1102 7 0.5161961150525229 1102 8 0.5699121342349187 1102 9 0.6882697075370459 1102 10-11 0.6847161399656017 1102 12-13 0.7220138602853936 1102 14-15 0.7262311088693494 1102 16-17 0.9264103909517942 1102 18-19 0.8397584430940057 1102 20-21 0.909124308745497 1102 22-23 0.6931157944883637 1102 24-25 0.7847659111810117 1102 26-27 0.7923760989666704 1102 28-29 0.910592653779176 1102 30-31 0.7201454908610359 1102 32-33 0.8775326960661687 1102 34-35 0.7193242799556856 1102 36-37 0.8364511391458223 1102 38-39 0.8576659511596105 1102 40-41 0.9790387037560748 1102 42-43 1.2264336490253172 1102 44-45 1.2287165094568309 1102 46-47 1.1235437966695372 1102 48-49 1.3562519942397273 1102 50-51 1.3826430655844604 1102 52-53 1.3156642383586785 1102 54-55 1.2380739836207084 1102 56-57 1.9788135651135725 1102 58-59 -7.7426569003277415 1102 60-61 -8.177386865024019 1102 62-63 -1.6208483814335146 1102 64-65 0.8879370538243272 1102 66-67 -0.4543530192615677 1102 68-69 -2.9108419807786277 1102 70-71 -3.6967887240997115 1102 72-73 -6.307692444263964 1102 74-75 -6.96739269765763 1102 76-77 -8.111630815749361 1102 78-79 -11.114479254366657 1102 80-81 -6.733443642902213 1102 82-83 -2.496564445388824 1102 84-85 -1.5886654778067602 1102 86-87 -1.3935740343230734 1102 88-89 -1.5825972185897896 1102 90-91 -6.965451843058975 1102 92-93 -13.09602327820719 1102 94-95 -11.347623566999852 1102 96-97 -11.358050089834943 1102 98-99 -10.989667363999972 1102 100-101 -10.765632616005803 1103 1 0.24627227419455622 1103 2 0.29406357132842587 1103 3 0.24032877965068167 1103 4 0.18750155928427148 1103 5 0.24064018306428636 1103 6 0.2206322090861903 1103 7 0.2921275673427033 1103 8 0.3300653265858884 1103 9 0.5093040895331526 1103 10-11 0.3647936564916421 1103 12-13 0.31818301611684774 1103 14-15 0.44871377751784536 1103 16-17 0.5002478156268566 1103 18-19 0.5763489354125255 1103 20-21 0.45260109405301563 1103 22-23 0.4260645746787475 1103 24-25 0.38228165492780875 1103 26-27 0.42007531459790926 1103 28-29 0.41993248632139313 1103 30-31 0.39424245439445116 1103 32-33 0.4912897511284413 1103 34-35 0.33155585396222165 1103 36-37 0.13412472301391176 1103 38-39 0.21338086797143063 1103 40-41 0.35045365070936896 1103 42-43 0.35466149101032585 1103 44-45 0.4147055526833938 1103 46-47 0.11282113740862343 1103 48-49 0.2958965548528596 1103 50-51 0.5635402693293656 1103 52-53 0.5308611818744637 1103 54-55 0.47946410411760354 1103 56-57 0.9582707653611529 1103 58-59 -5.889318213621976 1103 60-61 -7.8591290656282276 1103 62-63 -1.7143755559342821 1103 64-65 0.4259578859592885 1103 66-67 -1.2391652792513952 1103 68-69 -3.8031897260347947 1103 70-71 -4.436687301467829 1103 72-73 -7.3033202038776 1103 74-75 -7.882362659716179 1103 76-77 -8.64346861776815 1103 78-79 -11.24229869078999 1103 80-81 -7.523639953676987 1103 82-83 -3.620582833993044 1103 84-85 -2.6952806125115707 1103 86-87 -2.5376652686742354 1103 88-89 -2.709422101187574 1103 90-91 -7.758605355231765 1103 92-93 -13.22789225710125 1103 94-95 -11.62319435691373 1103 96-97 -11.72879145522102 1103 98-99 -11.378100743274137 1103 100-101 -11.17568330431298 1104 1 0.14002031118975822 1104 2 0.1009546204167151 1104 3 0.16039522431491804 1104 4 0.09636839098965311 1104 5 0.08079355592107618 1104 6 0.12202060214364252 1104 7 0.15838280771203017 1104 8 0.09987045995316635 1104 9 0.13760510766204703 1104 10-11 0.31386185103443864 1104 12-13 0.27139912514384434 1104 14-15 0.3377301194718072 1104 16-17 0.44134499143600436 1104 18-19 0.37402239675815707 1104 20-21 0.2689135907610236 1104 22-23 0.2781013201777256 1104 24-25 0.5136912923303356 1104 26-27 0.40166842588979534 1104 28-29 0.22549747080267935 1104 30-31 0.4203648054353053 1104 32-33 0.40103737455145705 1104 34-35 0.35928567427291114 1104 36-37 0.3707348356343019 1104 38-39 0.2993975435142886 1104 40-41 0.1466152325839829 1104 42-43 0.19514747735073712 1104 44-45 0.11917993559271878 1104 46-47 0.050717030363657045 1104 48-49 -0.1706918842437517 1104 50-51 -0.06251926555798093 1104 52-53 0.05543068638766613 1104 54-55 0.09590759786622982 1104 56-57 0.5473268063438148 1104 58-59 -6.491116960030045 1104 60-61 -8.331037369650609 1104 62-63 -2.5775563162438395 1104 64-65 -0.1958249808373047 1104 66-67 -1.959986493163811 1104 68-69 -4.2180284816506965 1104 70-71 -4.87310399105654 1104 72-73 -7.413508905943704 1104 74-75 -8.217241524031337 1104 76-77 -8.894819067527909 1104 78-79 -11.587935309096022 1104 80-81 -7.688764503283217 1104 82-83 -4.4168790219108836 1104 84-85 -3.566685509440404 1104 86-87 -3.3056688269557277 1104 88-89 -3.4107088569283235 1104 90-91 -8.585755589231688 1104 92-93 -13.998072921821006 1104 94-95 -11.545313543737164 1104 96-97 -11.374116236193577 1104 98-99 -10.777291546365005 1104 100-101 -10.440509477190055 1105 1 0.14484667203300816 1105 2 0.16721441674827275 1105 3 0.12972443257012856 1105 4 0.11135727641181603 1105 5 0.14540147424374794 1105 6 0.03257473699014923 1105 7 0.17085523996267682 1105 8 0.20253523709300225 1105 9 0.02008655015637828 1105 10-11 0.2539303658216383 1105 12-13 0.2912593254297988 1105 14-15 0.33061790743268915 1105 16-17 0.32654870361751875 1105 18-19 0.2579115075822145 1105 20-21 0.26805484747131914 1105 22-23 0.33323317396489927 1105 24-25 0.34641908285723844 1105 26-27 0.43995078379377617 1105 28-29 0.35201096332670545 1105 30-31 0.41547186404270775 1105 32-33 0.39176348161102936 1105 34-35 0.626869743151218 1105 36-37 0.3734252730150516 1105 38-39 0.5687518288231175 1105 40-41 0.5350046053563347 1105 42-43 0.5674090485156285 1105 44-45 0.5300140003401168 1105 46-47 0.6063455653291427 1105 48-49 0.6527022366451405 1105 50-51 0.8740158632146233 1105 52-53 0.7931249485050884 1105 54-55 0.8563937913930459 1105 56-57 1.206171959425621 1105 58-59 -6.074929721917588 1105 60-61 -7.953243538022615 1105 62-63 -1.7405977448971903 1105 64-65 0.2713551822413045 1105 66-67 -1.4837827216915827 1105 68-69 -3.6472511707467454 1105 70-71 -4.124138176523708 1105 72-73 -5.369896459171695 1105 74-75 -5.574554060905783 1105 76-77 -7.473612773770636 1105 78-79 -11.13102604623504 1105 80-81 -6.945381006122851 1105 82-83 -2.9410883414382276 1105 84-85 -2.0115580295579356 1105 86-87 -1.7634876189998856 1105 88-89 -1.9497921253820714 1105 90-91 -6.776708361752682 1105 92-93 -9.895230029561944 1105 94-95 -10.9185568135128 1105 96-97 -11.262565628658216 1105 98-99 -10.919465804672264 1105 100-101 -10.476410972968266 1106 1 0.07599088850668778 1106 2 0.1236981722102719 1106 3 0.05469319828268482 1106 4 0.06343991517109515 1106 5 0.06997134720379705 1106 6 -0.025121406209393626 1106 7 0.10318086842805485 1106 8 0.041729257050441504 1106 9 0.11611431703163788 1106 10-11 0.19025600216638594 1106 12-13 0.10566560803989233 1106 14-15 0.03827876585973655 1106 16-17 -0.14580187288100177 1106 18-19 0.08570729025165491 1106 20-21 0.20088647878016985 1106 22-23 0.06455206606736397 1106 24-25 0.11952174455531406 1106 26-27 0.35624141606834314 1106 28-29 0.1282897470091342 1106 30-31 0.01840293783737934 1106 32-33 -0.049794051774078696 1106 34-35 0.06548507314140295 1106 36-37 0.032894469876502797 1106 38-39 0.05167601802435229 1106 40-41 0.1353231778521291 1106 42-43 0.16505503956960155 1106 44-45 -0.11244857637902328 1106 46-47 -0.33685407655381283 1106 48-49 -0.39967312045005343 1106 50-51 -0.26753738576684327 1106 52-53 -0.4168876535511359 1106 54-55 -0.5488696723453899 1106 56-57 0.12356447137147342 1106 58-59 -7.068172675674099 1106 60-61 -9.131213725467735 1106 62-63 -2.9118286146655983 1106 64-65 -0.7165083826484526 1106 66-67 -2.2640868749530725 1106 68-69 -3.9861462673698966 1106 70-71 -4.53061629331655 1106 72-73 -6.038378662399181 1106 74-75 -6.277863048648941 1106 76-77 -8.319313916426012 1106 78-79 -11.526802018216026 1106 80-81 -7.677983003245899 1106 82-83 -4.101061325072298 1106 84-85 -3.146025515120236 1106 86-87 -3.062137145425549 1106 88-89 -3.128822087152386 1106 90-91 -8.034547219914792 1106 92-93 -10.97822089787267 1106 94-95 -11.162536610826038 1106 96-97 -11.67238582651887 1106 98-99 -11.10392587870873 1106 100-101 -10.82444563822584 1107 1 0.164998195838038 1107 2 0.23025000820512886 1107 3 0.23158955105518686 1107 4 0.01365844176942943 1107 5 0.07068598489536271 1107 6 0.1093696434859126 1107 7 0.1645956331498155 1107 8 0.016627298606401553 1107 9 0.20802771859314362 1107 10-11 0.22723540742736503 1107 12-13 0.1892695511759328 1107 14-15 0.3605372784921883 1107 16-17 0.32449113847743405 1107 18-19 0.340576409533746 1107 20-21 0.2533541578915006 1107 22-23 0.3472373308126464 1107 24-25 0.38180071078211597 1107 26-27 0.23175752265484562 1107 28-29 0.3195023777227064 1107 30-31 0.24425964877064388 1107 32-33 0.1470321743891887 1107 34-35 0.03089225071871482 1107 36-37 0.01573969856799806 1107 38-39 0.1766913086956876 1107 40-41 -0.13129165323238112 1107 42-43 -0.1758288726631818 1107 44-45 -0.10561271771187108 1107 46-47 -0.2015047682864477 1107 48-49 -0.13565163674731195 1107 50-51 -0.11574175332734171 1107 52-53 -0.11226306119919371 1107 54-55 -0.15200060754018807 1107 56-57 0.6727880519390688 1107 58-59 -6.343328739570534 1107 60-61 -8.340927697055562 1107 62-63 -2.2779172485586408 1107 64-65 -0.3269751257478397 1107 66-67 -1.3297275602722962 1107 68-69 -4.171840141939345 1107 70-71 -4.937149805268037 1107 72-73 -5.905692778598379 1107 74-75 -6.291342858772232 1107 76-77 -8.792248066252304 1107 78-79 -11.909086382606962 1107 80-81 -7.514394363162303 1107 82-83 -3.606061560313396 1107 84-85 -2.7675508183955344 1107 86-87 -2.602112531778097 1107 88-89 -2.7067168526665384 1107 90-91 -7.829095569388965 1107 92-93 -10.463176363018608 1107 94-95 -11.26674173115805 1107 96-97 -11.974490120040329 1107 98-99 -11.385226192446066 1107 100-101 -10.992813139296805 1108 1 0.010160772856231404 1108 2 0.1477742574977121 1108 3 -0.048690975836070294 1108 4 -0.04557834488841195 1108 5 -0.10360577621097633 1108 6 0.08128175945078908 1108 7 0.062219742132022304 1108 8 0.027564937919585475 1108 9 -0.06596512041596014 1108 10-11 0.1727292858594538 1108 12-13 0.053974218959453424 1108 14-15 0.09673008089858115 1108 16-17 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2.7527468263523716 2312 82-83 1.3007308695560766 2312 84-85 1.0321537409900579 2312 86-87 0.9621076041561345 2312 88-89 1.3046746253027415 2312 90-91 2.5138325603434915 2312 92-93 4.450902700585633 2312 94-95 8.170477025602604 2312 96-97 8.937458886594127 2312 98-99 9.347425742002626 2312 100-101 8.715219645371834 2313 1 -0.31823693250000673 2313 2 -0.2811327920746578 2313 3 -0.44356205738644405 2313 4 -0.31275775877249146 2313 5 -0.47249621913711337 2313 6 -0.32614894906258485 2313 7 -0.389817932495113 2313 8 -0.34308911041834733 2313 9 -0.44590255623747765 2313 10-11 -0.5923440956093273 2313 12-13 -0.48087846936902423 2313 14-15 -0.49180006075985006 2313 16-17 -0.48224527901339087 2313 18-19 -0.5599116349606987 2313 20-21 -0.6310800296706063 2313 22-23 -0.6426309922474545 2313 24-25 -0.5483575414934307 2313 26-27 -0.7420721099762275 2313 28-29 -0.6938769682161734 2313 30-31 -0.8452897249885964 2313 32-33 -0.6774483728725826 2313 34-35 -0.8927070547912521 2313 36-37 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3.5764108566573256 2314 60-61 3.489331581376163 2314 62-63 1.1508149429292835 2314 64-65 0.9820793632350764 2314 66-67 1.9971692009979876 2314 68-69 0.038706548434493726 2314 70-71 2.9823095811963665 2314 72-73 8.212134864263586 2314 74-75 9.401174752805268 2314 76-77 8.47298229093074 2314 78-79 6.092456459102657 2314 80-81 3.1807036539878872 2314 82-83 1.6836134413746144 2314 84-85 1.2851601266653496 2314 86-87 1.5041218541236354 2314 88-89 1.6733072296183522 2314 90-91 2.9067949124818 2314 92-93 4.79968852861111 2314 94-95 8.230663233843867 2314 96-97 8.682327192196622 2314 98-99 9.09048558536672 2314 100-101 8.463018374736189 2315 1 -0.5883549052712951 2315 2 -0.47717901253208694 2315 3 -0.4164887748099666 2315 4 -0.24522568937997136 2315 5 -0.7093365617373237 2315 6 -0.5840364137901162 2315 7 -0.6542824350395406 2315 8 -0.6489123522049951 2315 9 -1.1221416653532756 2315 10-11 -0.9263760799872571 2315 12-13 -0.5375969405490721 2315 14-15 -0.7482341194937732 2315 16-17 -0.8467178536501976 2315 18-19 -0.7030877972131933 2315 20-21 -0.9456011656716257 2315 22-23 -0.832760111376075 2315 24-25 -0.746659439465617 2315 26-27 -0.681744168342604 2315 28-29 -0.6431737086365814 2315 30-31 -0.7177549591737318 2315 32-33 -0.5738999583517383 2315 34-35 -0.7979155723944231 2315 36-37 -1.034196119315844 2315 38-39 -1.0696254430734697 2315 40-41 -1.002752163468358 2315 42-43 -1.112128951292199 2315 44-45 -0.9500115846962203 2315 46-47 -1.1116950410249231 2315 48-49 -1.099565656395086 2315 50-51 -1.1006419039540063 2315 52-53 -0.9100370548899193 2315 54-55 -0.9954755836199105 2315 56-57 0.378041748923696 2315 58-59 3.5367189115061173 2315 60-61 3.000262064120651 2315 62-63 0.768969089887154 2315 64-65 0.5986825633170447 2315 66-67 1.8377508167490895 2315 68-69 6.320890937362279 2315 70-71 5.140597947173722 2315 72-73 8.4295735179751 2315 74-75 9.377203167620593 2315 76-77 7.950185549071829 2315 78-79 5.192987102363528 2315 80-81 2.6164168419457425 2315 82-83 1.0297695862491913 2315 84-85 0.7299681046711868 2315 86-87 0.6081731852142944 2315 88-89 0.770411104210659 2315 90-91 1.9798956108988364 2315 92-93 3.547375555813513 2315 94-95 7.1713421839984015 2315 96-97 8.150700768467518 2315 98-99 8.34611860051332 2315 100-101 7.7472758156951915 2316 1 -0.4534088280123747 2316 2 -0.5046758621302985 2316 3 -0.5770825139888274 2316 4 -0.38196294408594156 2316 5 -0.5266132580457352 2316 6 -0.5162367329356314 2316 7 -0.6061312657317686 2316 8 -0.616396278908617 2316 9 -0.7406494280146774 2316 10-11 -0.5819055259806234 2316 12-13 -0.5988932282512636 2316 14-15 -0.6487876263461132 2316 16-17 -0.8552917658244894 2316 18-19 -0.8275741835506167 2316 20-21 -0.7753192716465023 2316 22-23 -0.8681195784673221 2316 24-25 -2.1579613496979064 2316 26-27 -1.419704367969203 2316 28-29 -0.7974171124270555 2316 30-31 -0.4803788779522691 2316 32-33 -0.6135802952037679 2316 34-35 -0.7028702708661712 2316 36-37 -0.6029493991250732 2316 38-39 -1.1224111836231572 2316 40-41 -0.9150264144223996 2316 42-43 -0.9159588760038417 2316 44-45 -0.9847552508714301 2316 46-47 -0.8797937869329715 2316 48-49 -0.9248495495420315 2316 50-51 -1.0102588400856334 2316 52-53 -0.7953155753969909 2316 54-55 -0.6942164046163981 2316 56-57 0.32133674790628675 2316 58-59 3.3815852737826866 2316 60-61 3.462350146855737 2316 62-63 1.1568107701490398 2316 64-65 0.6634451854508931 2316 66-67 5.425666660860426 2316 68-69 8.337908461806897 2316 70-71 5.397083937256651 2316 72-73 8.661727339689133 2316 74-75 9.271629009125931 2316 76-77 7.994774890182697 2316 78-79 5.576694884302174 2316 80-81 2.7494557212951705 2316 82-83 1.4214725167475741 2316 84-85 0.9424328076766528 2316 86-87 0.917994228113308 2316 88-89 1.0630932474120378 2316 90-91 2.217641172669918 2316 92-93 3.628603266497752 2316 94-95 7.4119900422606655 2316 96-97 8.180716600798315 2316 98-99 8.329163102392853 2316 100-101 7.31977878760396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 27.0 5 96.0 6 253.0 7 593.0 8 1159.0 9 2178.0 10 4080.0 11 6336.0 12 9018.0 13 11811.0 14 14553.0 15 20109.0 16 25669.0 17 32454.0 18 30370.0 19 25170.0 20 25096.0 21 25152.0 22 23937.0 23 24167.0 24 24953.0 25 25568.0 26 32023.0 27 44935.0 28 61657.0 29 80549.0 30 96042.0 31 104593.0 32 107433.0 33 112364.0 34 116130.0 35 115657.0 36 100744.0 37 69192.0 38 25140.0 39 1373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.81412514630825 24.123953704107056 15.921765168389276 20.140155981195417 2 14.129546908559156 24.370230315770232 41.69260955993237 19.807613215738247 3 15.997618029290528 32.037220434395486 32.94488265438362 19.020278881930366 4 10.124790088762465 20.837617236140147 42.04379291042645 26.99379976467094 5 9.264525366787035 42.249279429774234 36.52844484011701 11.957750363321725 6 23.619886176621154 43.612589256963666 19.52825628609637 13.239268280318809 7 22.86547402812525 37.31464980522522 24.773595736659924 15.046280429989602 8 24.030764221883953 36.76298811641968 22.07829854318329 17.12794911851308 9 26.416686658213102 16.73071101362852 23.634220386807865 33.218381941350515 10-11 21.422941156311044 30.06061008487839 30.00670430789266 18.509744450917896 12-13 23.23285439299953 29.055606486400038 30.12290517152747 17.588633949072964 14-15 20.946309692786173 29.573686194655384 29.41100452095834 20.0689995916001 16-17 17.12458017752493 32.665615682511394 30.69484189542708 19.514962244536598 18-19 17.04558882486353 30.497685412439974 35.022510421623714 17.434215341072782 20-21 18.213726766967113 29.74399396826485 35.12761032477695 16.91466893999109 22-23 18.12562116590699 29.726537064327086 34.717533728594766 17.43030804117116 24-25 17.433163976375702 30.550974159498267 35.05182094428642 16.96404091983961 26-27 17.041145888927485 30.560881685890116 35.35732632580781 17.040646099374584 28-29 16.769214896464664 29.87807381401946 35.959424815993316 17.393286473522565 30-31 17.397021456325273 30.39970380938406 35.31833886191541 16.884935872375262 32-33 16.93823703393252 30.937959293539823 35.02388460279376 17.0999190697339 34-35 17.15217472792473 30.62283236736275 35.16588692878474 17.05910597592779 36-37 17.461821770892776 30.086865603634887 34.668931566644694 17.782381058827646 38-39 16.665369260764304 31.0144404847775 34.82088656863515 17.499303685823044 40-41 16.714891799656264 30.5149929992319 35.4977330080949 17.272382193016945 42-43 16.80039223150229 30.36623152442859 34.72366497916181 18.109711264907315 44-45 16.847928946329084 30.54151570404112 34.50407483032551 18.10648051930429 46-47 17.270553180639588 30.623929368400116 33.84172525196111 18.26379219899919 48-49 16.914180134477537 30.970971521458807 33.522252286257384 18.592596057806272 50-51 16.969550457441233 30.81730238756437 32.94608867300441 19.26705848198998 52-53 16.859954630680672 31.274372261942297 33.04629663022291 18.819376477154123 54-55 17.135428419630436 30.90528408490412 32.82143969104999 19.137847804415447 56-57 17.409650615677243 30.996310226963946 32.07310184059609 19.520937316762723 58-59 16.409532375329636 30.475421231892685 33.43930135903993 19.675745033737748 60-61 17.90381160644493 31.290106695761494 31.4033659814653 19.402715716328277 62-63 16.847400798524045 30.913069119738623 32.63120955258664 19.608320529150696 64-65 16.479024937433888 30.439998555822985 32.84398133865915 20.236995168083975 66-67 18.243641156002745 30.51097701654923 30.576600583409075 20.66878124403895 68-69 20.058774678123388 29.917997774408633 29.32156128717645 20.701666260291525 70-71 19.214608254368464 30.08533203181284 30.201002477193295 20.4990572366254 72-73 18.69593508939023 30.104549122819773 30.15885941123867 21.040656376551333 74-75 18.67584599090815 30.454259114142534 29.65366584582418 21.21622904912514 76-77 16.670161588082667 30.714367952785715 31.351033155314912 21.264437303816706 78-79 16.770358432870143 30.724138561400853 30.688760175766415 21.81674282996259 80-81 16.978690378612413 31.012867452812674 30.186964332123367 21.821477836451546 82-83 17.439031094730726 30.631676199547194 29.98145068760259 21.94784201811949 84-85 17.077927432802497 30.309087267772224 29.837929189203276 22.775056110222003 86-87 17.0873820374981 30.549012495796298 30.07366386515022 22.289941601555388 88-89 17.71511617944412 29.96939865428333 29.95758222237454 22.357902943898008 90-91 17.661821922096507 29.92263315594115 29.774160368498727 22.64138455346362 92-93 17.665571711684265 30.00730923574145 29.775301792050097 22.55181726052419 94-95 17.526967666275155 29.424119806968662 29.533078614104706 23.515833912651477 96-97 17.489198297749304 29.36516168670998 29.733440552237596 23.412199463303125 98-99 17.316366687741006 29.473352975482875 29.589129677836546 23.62115065893957 100-101 16.746730923533587 29.393646296480124 30.231857248267048 23.62776553171924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2379.0 1 1715.5 2 1062.5 3 1109.0 4 1150.0 5 1254.0 6 1745.5 7 2630.0 8 3463.0 9 3993.5 10 4088.0 11 4119.5 12 4418.5 13 4677.5 14 4841.0 15 4981.0 16 5130.0 17 5339.0 18 5547.5 19 5876.5 20 6398.5 21 7143.0 22 8002.0 23 8955.5 24 10168.5 25 11906.0 26 14575.0 27 17620.5 28 20375.5 29 23677.0 30 28130.5 31 33749.5 32 41425.5 33 50476.0 34 58577.0 35 65704.0 36 72038.0 37 74656.0 38 73721.5 39 71542.5 40 68164.5 41 63819.5 42 59747.5 43 57134.5 44 54454.0 45 50757.5 46 46120.0 47 40946.5 48 36233.0 49 31312.0 50 26137.0 51 21217.5 52 16680.5 53 13011.5 54 10218.0 55 8022.0 56 6361.5 57 5126.0 58 4218.0 59 3445.0 60 2747.0 61 2125.5 62 1541.5 63 1014.5 64 639.0 65 434.5 66 317.0 67 239.0 68 186.5 69 147.0 70 128.0 71 121.0 72 106.0 73 116.5 74 125.5 75 103.0 76 75.5 77 45.0 78 26.0 79 16.0 80 10.0 81 8.5 82 8.0 83 5.0 84 3.5 85 2.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021062522133497835 2 0.005711870409084159 3 0.0050692849880621905 4 0.0 5 0.0718267703942333 6 7.139838011355199E-5 7 0.0 8 2.8559352045420794E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8559352045420794E-4 16-17 0.0 18-19 0.0013208700321007119 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7849595028387996E-4 28-29 0.0022490489735768875 30-31 0.010174269166181159 32-33 2.49894330397432E-4 34-35 0.0010352765116465037 36-37 0.027345579583490407 38-39 0.02584621360110582 40-41 0.027738270674114943 42-43 0.028452254475250467 44-45 0.029344734226669868 46-47 0.03038001073831637 48-49 0.030879799399111233 50-51 0.025524920890594834 52-53 0.021883603504803683 54-55 0.016850017706798268 56-57 0.15222134640209284 58-59 0.7307981196522613 60-61 0.3026220341112901 62-63 5.711870409084159E-4 64-65 0.13362206838251253 66-67 1.3728837520134343 68-69 2.6352785108011467 70-71 2.133919085643785 72-73 1.796526040417195 74-75 1.5075767960976503 76-77 1.0887181991614974 78-79 0.40500731119412364 80-81 2.8559352045420794E-4 82-83 7.139838011355199E-5 84-85 3.569919005677599E-5 86-87 0.0029273335846556315 88-89 0.0 90-91 0.21630139255400574 92-93 0.16857157544809623 94-95 1.2495073511772166 96-97 1.2230185521550887 98-99 0.959522830346025 100-101 0.3825882198384683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1400592.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.508375444722308 #Duplication Level Percentage of deduplicated Percentage of total 1 74.54338825094902 22.74197675681654 2 11.576129688205246 7.0633782144912365 3 4.675225084234735 4.2790056647525025 4 2.381786452586442 2.906577412986419 5 1.4158814499113535 2.159812142955668 6 0.8829912525835074 1.6163177188933964 7 0.6209780126153898 1.326152124625147 8 0.4554793003579024 1.111674680209466 9 0.35787223917665306 0.9826290570640313 >10 2.315580620192773 14.095259856580448 >50 0.3746222202746844 8.06038615153527 >100 0.3643327899846781 21.958223126449816 >500 0.02825914581858992 5.709366009289682 >1k 0.006539306470417502 2.821266148806118 >5k 4.6709331931553584E-4 1.1009037090919116 >10k+ 4.6709331931553584E-4 2.067071225452427 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16220 1.1580817254418132 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12791 0.9132566800324433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9777 0.6980619623701978 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5674 0.40511440876429394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3054 0.21805065286678776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2197 0.1568622411094737 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.14986519985834562 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1929 0.13772747523904177 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.12844568582428 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1617 0.11545118064361355 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1559 0.11131007459702755 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1467 0.10474142362658077 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1430 0.10209968356237933 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4279676022710397E-4 0.0 10-11 0.0 0.0 0.0 2.498943303974319E-4 0.0 12-13 0.0 0.0 0.0 4.640894707380879E-4 0.0 14-15 0.0 0.0 0.0 4.997886607948638E-4 0.0 16-17 0.0 0.0 0.0 5.711870409084159E-4 0.0 18-19 0.0 0.0 0.0 7.496829911922958E-4 0.0 20-21 0.0 0.0 0.0 0.0011780732718736078 0.0 22-23 0.0 0.0 0.0 0.002963032774712407 0.0 24-25 0.0 0.0 0.0 0.00738973234175263 0.0 26-27 0.0 0.0 0.0 0.015564846864754333 0.0 28-29 0.0 0.0 0.0 0.039376206632623915 0.0 30-31 0.0 0.0 0.0 0.11180986325782241 0.0 32-33 0.0 0.0 0.0 0.24657430572215178 0.0 34-35 0.0 0.0 0.0 0.44577578623896175 0.0 36-37 0.0 0.0 0.0 0.7498614871425797 0.0 38-39 0.0 0.0 0.0 1.1988145012965945 0.0 40-41 0.0 0.0 0.0 1.7188803020437073 0.0 42-43 0.0 0.0 0.0 2.198998709117287 0.0 44-45 0.0 0.0 0.0 2.7182077293030376 0.0 46-47 0.0 0.0 0.0 3.306387584678479 0.0 48-49 0.0 7.139838011355199E-5 0.0 3.872398243028662 0.0 50-51 0.0 7.139838011355199E-5 0.0 4.456579789117745 0.0 52-53 0.0 7.139838011355199E-5 0.0 4.974824931171962 0.0 54-55 0.0 7.139838011355199E-5 0.0 5.499603025006568 0.0 56-57 0.0 7.139838011355199E-5 0.0 5.964442178735849 0.0 58-59 0.0 7.139838011355199E-5 0.0 6.286377474667855 0.0 60-61 0.0 7.139838011355199E-5 0.0 6.5268829180803545 0.0 62-63 0.0 7.139838011355199E-5 0.0 6.724942024515348 0.0 64-65 0.0 7.139838011355199E-5 0.0 6.9259641637250535 0.0 66-67 0.0 7.139838011355199E-5 0.0 7.084290071626855 0.0 68-69 0.0 7.139838011355199E-5 0.0 7.259715891565852 0.0 70-71 0.0 7.139838011355199E-5 0.0 7.593110627506083 0.0 72-73 0.0 7.139838011355199E-5 0.0 7.980946628282898 0.0 74-75 0.0 7.139838011355199E-5 0.0 8.417547722677268 0.0 76-77 0.0 7.139838011355199E-5 0.0 9.020864034636784 0.0 78-79 0.0 7.139838011355199E-5 0.0 9.880643327964176 0.0 80-81 0.0 1.0709757017032798E-4 0.0 10.830063287524133 0.0 82-83 0.0 1.4279676022710397E-4 0.0 11.707120988839005 0.0 84-85 0.0 1.4279676022710397E-4 0.0 12.396115356934782 0.0 86-87 0.0 1.4279676022710397E-4 0.0 13.00293018951986 0.0 88-89 0.0 1.4279676022710397E-4 0.0 13.5940016792899 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6850 0.0 48.818806 1 GTATCAA 11620 0.0 36.896847 1 CATTCCG 420 0.0 34.780884 9 CGTCCCG 30 0.0037441233 32.017952 90-91 CGGAGTC 50 4.142437E-5 29.259031 66-67 CATGGGT 1190 0.0 28.925661 4 GTACATG 10440 0.0 28.417225 1 ATCAACG 15090 0.0 28.15519 3 TCAACGC 15210 0.0 27.93206 4 TATCAAC 15230 0.0 27.897373 2 CAACGCA 15390 0.0 27.605371 5 GGCATTC 550 0.0 27.41672 7 AACGCAG 15520 0.0 27.356546 6 TACATGG 10855 0.0 27.06429 2 ACATGGG 10305 0.0 26.540121 3 TTAGGCA 660 0.0 25.719702 4 CATGGGA 5280 0.0 25.362484 4 ATGGGAT 2445 0.0 25.263866 5 CGGACAT 50 0.0014194781 24.382526 66-67 CCTTTAG 640 0.0 24.320107 1 >>END_MODULE