FastQCFastQC Report
Fri 27 May 2016
SRR939870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456600
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164311.1280378964712343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118390.8127831937388438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88540.6078539063572703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51070.3506110119456268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.18131264588768364No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.14204311410133186No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.1416998489633393No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.13531511739667718No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.11938761499382122No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15170.10414664286695044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.044.349451
GTATCAA128100.039.3254431
GTATCGA500.00169573137.629051
GTCGGGA2550.035.046672
CATTCCG3200.033.810849
CATGGGT11300.033.7174574
GTACATG107100.030.9183641
TATCAAC163250.030.6276322
ATCAACG163650.030.4675883
TCAACGC163450.030.4473134
CAACGCA164100.030.15685
AACGCAG165450.029.9391656
AACGCCG804.7570534E-429.400736
ACATGGG104500.029.0329823
TACATGG112550.028.9614982
CATGGGG33950.027.9872234
GGTCGGG3250.027.4981521
ATGGGCG1555.0424205E-827.3142245
CCGTATC1154.656613E-1027.11704894-95
ACGCAGA185850.026.6022437
Produced by FastQC (version 0.11.5)