FastQCFastQC Report
Fri 27 May 2016
SRR939870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456600
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162801.1176712893038583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134990.9267472195523824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99590.6837155018536317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59770.4103391459563367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.228889194013456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.16572840862282026No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.1511053137443361No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.13675683097624605No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT18650.1280378964712344No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.12117259371138267No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT15790.10840313057805849No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.10325415350816972No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14880.10215570506659344No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA14690.10085129754222161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCG301.4128764E-647.88215364-65
GGTATCA74750.045.9548031
GTATCAA124400.036.957871
TACTCGC1509.713403E-1031.619017
CATGGGT11550.029.9764634
GTCGCGT504.692217E-528.72929464-65
GTACATG109550.028.6714171
GTATGGT3150.028.6184831
TCAACGC162350.027.9575564
TCGCGTA605.06001E-627.93125566-67
TATCAAC162850.027.9065882
ATCAACG163250.027.8324763
CAACGCA164100.027.688315
CATGGGG32550.027.6848494
ACATGGG106800.027.6666343
AACGCAG165550.027.4744456
GGATGAC4850.027.381418
TACATGG113800.027.1373672
GTACACG700.0084386127.1122471
GACCGGT1056.9782596E-527.1020096
Produced by FastQC (version 0.11.5)