##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939870_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456600 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37149114375944 31.0 31.0 34.0 30.0 34.0 2 31.584486475353565 31.0 31.0 34.0 30.0 34.0 3 31.591982699437047 31.0 31.0 34.0 30.0 34.0 4 35.43748798572017 37.0 35.0 37.0 33.0 37.0 5 35.204618289166554 37.0 35.0 37.0 32.0 37.0 6 35.245812851846765 37.0 35.0 37.0 32.0 37.0 7 35.150757242894414 37.0 35.0 37.0 32.0 37.0 8 35.12544555814912 37.0 35.0 37.0 32.0 37.0 9 36.6424529726761 39.0 35.0 39.0 32.0 39.0 10-11 36.52692709048469 38.0 35.0 39.0 32.0 39.0 12-13 36.46831937388439 38.0 35.0 39.0 32.0 39.0 14-15 37.36527873129205 39.0 36.0 41.0 32.0 41.0 16-17 37.32220170259508 39.0 36.0 41.0 32.0 41.0 18-19 37.44607682273788 39.0 36.0 41.0 32.0 41.0 20-21 37.4368766305094 39.0 36.0 41.0 32.0 41.0 22-23 37.2873798572017 39.0 36.0 40.5 31.5 41.0 24-25 37.14493718247975 39.0 36.0 40.5 31.0 41.0 26-27 36.83294006590691 39.0 35.5 40.0 31.0 41.0 28-29 36.60313229438418 38.5 35.0 40.0 30.0 41.0 30-31 36.24046203487573 38.0 35.0 40.0 30.0 41.0 32-33 36.016663119593574 38.0 35.0 40.0 30.0 41.0 34-35 35.79000549224221 38.0 35.0 40.0 30.0 41.0 36-37 35.64376046958671 38.0 35.0 40.0 29.0 41.0 38-39 35.456241933269254 38.0 34.0 40.0 27.0 41.0 40-41 35.314880886997116 38.0 34.0 40.0 27.0 41.0 42-43 34.975904160373474 38.0 34.0 40.0 25.5 41.0 44-45 34.536880749691065 38.0 33.5 40.0 24.5 41.0 46-47 34.016440683784154 38.0 33.0 40.0 22.0 41.0 48-49 33.55985960455856 38.0 33.0 40.0 18.5 41.0 50-51 33.2979373197858 38.0 33.0 40.0 15.0 41.0 52-53 33.08276911986819 38.0 33.0 40.0 12.5 41.0 54-55 32.83797301936015 37.5 32.5 40.0 9.0 41.0 56-57 32.499353975010294 37.0 31.5 40.0 8.0 41.0 58-59 32.17798057119319 37.0 31.0 40.0 4.5 41.0 60-61 31.896491487024576 36.5 31.0 40.0 2.0 41.0 62-63 31.61875875326102 36.0 31.0 39.0 2.0 41.0 64-65 31.22143519154195 36.0 30.0 39.0 2.0 40.0 66-67 30.383746395716052 35.5 28.5 39.0 2.0 40.0 68-69 28.59072806535768 34.0 24.5 38.0 2.0 40.0 70-71 27.969557187971986 34.0 23.5 37.0 2.0 39.5 72-73 28.44508684607991 34.0 26.0 37.0 2.0 39.0 74-75 28.46905396127969 34.0 26.0 36.5 2.0 39.0 76-77 27.900389949196757 32.5 26.0 36.0 2.0 38.5 78-79 28.331742757105587 34.0 27.0 36.0 2.0 38.5 80-81 28.06803720994096 34.0 26.0 36.0 2.0 37.5 82-83 27.569811547439244 33.5 26.0 35.0 2.0 37.0 84-85 27.14575072085679 33.0 26.0 35.0 2.0 36.5 86-87 26.738816765069338 33.0 25.0 35.0 2.0 36.0 88-89 26.24087704242757 33.0 24.0 35.0 2.0 36.0 90-91 25.72077783880269 32.0 22.0 35.0 2.0 35.0 92-93 25.221569408210904 32.0 19.5 34.5 2.0 35.0 94-95 24.435311684745297 31.0 12.5 34.0 2.0 35.0 96-97 23.907271728683234 31.0 4.5 34.0 2.0 35.0 98-99 23.27195661128656 31.0 2.0 34.0 2.0 35.0 100-101 21.888496155430452 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31178524141581576 1101 2 0.2994452654669679 1101 3 0.3810686302519706 1101 4 0.33440654445792006 1101 5 0.18015419537690747 1101 6 0.06033738279578671 1101 7 0.23278470976283216 1101 8 0.2880743315383185 1101 9 0.40853746614669006 1101 10-11 0.40829687860178865 1101 12-13 0.3851709354290378 1101 14-15 0.3739614649113747 1101 16-17 0.5117310239066271 1101 18-19 0.3601914357986047 1101 20-21 0.4401590237103008 1101 22-23 0.27269970246224773 1101 24-25 0.3044422337905033 1101 26-27 0.36113006492562505 1101 28-29 0.34260466654215094 1101 30-31 0.2511530555212218 1101 32-33 0.23706581839671514 1101 34-35 0.36551896990180666 1101 36-37 0.5025080272933948 1101 38-39 0.5596570651646005 1101 40-41 0.4635082317675625 1101 42-43 0.2936847068545205 1101 44-45 0.17575751192439526 1101 46-47 0.4201924541128861 1101 48-49 0.5581495748209093 1101 50-51 0.5409240042335526 1101 52-53 0.22342235732185145 1101 54-55 -0.05912006124190583 1101 56-57 -0.044480868826369147 1101 58-59 0.013979781483058673 1101 60-61 -0.17684535698660042 1101 62-63 -0.16049824000893054 1101 64-65 -0.31844150812355565 1101 66-67 -0.514457836474314 1101 68-69 -8.67344472433022 1101 70-71 -5.910559602569677 1101 72-73 -1.6187712893577455 1101 74-75 -0.7034811477897058 1101 76-77 -0.5344956294336818 1101 78-79 -0.739116316676931 1101 80-81 -0.7387334307843787 1101 82-83 -0.5971776280219636 1101 84-85 -0.6571868089638038 1101 86-87 -0.7351786930793551 1101 88-89 -0.8082626174979985 1101 90-91 -1.2080603190096468 1101 92-93 -1.2311764720959353 1101 94-95 -1.0831456231166143 1101 96-97 -1.0421690989996577 1101 98-99 -0.7290721201466503 1101 100-101 -0.43795371848534614 1102 1 0.2552389930232195 1102 2 0.2464283291550764 1102 3 0.2973559992921153 1102 4 0.1760809366347189 1102 5 0.36899806032502624 1102 6 0.35482984170106135 1102 7 0.4378955085307368 1102 8 0.31836829965937596 1102 9 0.3990335934232263 1102 10-11 0.491020644264637 1102 12-13 0.34844336183733304 1102 14-15 0.46428638491497054 1102 16-17 0.5253199470203569 1102 18-19 0.5077282441162438 1102 20-21 0.36021569406528187 1102 22-23 0.4865565263305811 1102 24-25 0.4235232469786965 1102 26-27 0.5243112804723395 1102 28-29 0.4594128844912504 1102 30-31 0.37361609767759063 1102 32-33 0.34085131836182825 1102 34-35 0.12386900030542591 1102 36-37 0.4625673891164226 1102 38-39 0.60018833105174 1102 40-41 0.39293071250363454 1102 42-43 0.3735642387384459 1102 44-45 0.4385634172046551 1102 46-47 0.543383937111777 1102 48-49 0.5402792238335437 1102 50-51 0.5348888855597451 1102 52-53 0.4109735699777275 1102 54-55 0.14989236935311112 1102 56-57 0.2582527157106469 1102 58-59 0.16010880248647652 1102 60-61 0.18550449398582103 1102 62-63 0.34176932182007036 1102 64-65 0.4658540250363288 1102 66-67 0.6787138094082401 1102 68-69 -3.6045962240760296 1102 70-71 0.6696458959998566 1102 72-73 0.6474223767207867 1102 74-75 0.7873701037429335 1102 76-77 0.6362790149436428 1102 78-79 0.43072229492429415 1102 80-81 0.5179506307405859 1102 82-83 0.45627916394749235 1102 84-85 0.35781239356418126 1102 86-87 0.1888419116037987 1102 88-89 0.02373270732232058 1102 90-91 0.2310564308931653 1102 92-93 0.13281492517471705 1102 94-95 0.261222978416523 1102 96-97 -0.03616712525642285 1102 98-99 0.07544281750100623 1102 100-101 0.03379384174871092 1103 1 0.19575577944793565 1103 2 0.19205005610672643 1103 3 0.17808510252761067 1103 4 0.1731884753023607 1103 5 0.2720447862617732 1103 6 0.15745233997573393 1103 7 0.19523322622651307 1103 8 0.2379477256326652 1103 9 0.326407441513183 1103 10-11 0.2879800077201651 1103 12-13 0.22978140430068805 1103 14-15 0.17554262654205388 1103 16-17 0.34783446007195806 1103 18-19 0.476824578302093 1103 20-21 0.40529242040798863 1103 22-23 0.3061126096334732 1103 24-25 0.34136719615256794 1103 26-27 0.429408101809571 1103 28-29 0.4178527821894633 1103 30-31 0.45734016701546665 1103 32-33 0.5165404345880376 1103 34-35 0.3963693047750354 1103 36-37 0.4278223316731484 1103 38-39 0.47984671614490537 1103 40-41 0.4107574083993839 1103 42-43 0.38187118470523984 1103 44-45 0.5653851603946833 1103 46-47 0.13606448759284007 1103 48-49 0.268854711999829 1103 50-51 0.2700865878290628 1103 52-53 0.08771310637199292 1103 54-55 0.26220106094568507 1103 56-57 0.46216109065482414 1103 58-59 0.3686045196139034 1103 60-61 0.18163772988887317 1103 62-63 0.1528205539071088 1103 64-65 0.03515638366093654 1103 66-67 0.24123157013572083 1103 68-69 -3.297401607098802 1103 70-71 0.6091833051664217 1103 72-73 0.712040977784401 1103 74-75 0.5922000067186168 1103 76-77 0.4445037541146739 1103 78-79 0.22248537696870585 1103 80-81 0.13312641119769708 1103 82-83 0.1303495167262838 1103 84-85 0.06219777090351997 1103 86-87 -0.10330175745802705 1103 88-89 -0.12528994521695225 1103 90-91 -0.1261422456789134 1103 92-93 -0.0730522042857622 1103 94-95 -0.4432029948713385 1103 96-97 -0.5225268865522459 1103 98-99 -0.07007843447804518 1103 100-101 -0.00400098632744772 1104 1 0.15125383190635588 1104 2 0.13972928466321832 1104 3 0.2430668908645366 1104 4 0.1735253194252735 1104 5 0.24825194022960773 1104 6 0.15307289658128553 1104 7 0.18423012134114458 1104 8 0.05995778773507254 1104 9 0.20338229001553998 1104 10-11 0.21438473462508512 1104 12-13 0.07611400803998691 1104 14-15 0.20259304181870874 1104 16-17 0.1624966693090002 1104 18-19 0.10773291582835753 1104 20-21 0.21586622521034116 1104 22-23 0.0936753098548877 1104 24-25 0.151219158563201 1104 26-27 0.2825334937960591 1104 28-29 0.047323542287379894 1104 30-31 -0.16432797716132796 1104 32-33 0.008669228371303461 1104 34-35 -0.08093599861722822 1104 36-37 -0.012283306778002157 1104 38-39 0.028601731810276476 1104 40-41 0.05862704549564057 1104 42-43 -1.7535240093735638E-4 1104 44-45 0.0015590715589794968 1104 46-47 -0.12837012870787845 1104 48-49 0.06176909956599985 1104 50-51 0.05326420709748447 1104 52-53 0.05612962423060708 1104 54-55 -0.11661069204843955 1104 56-57 0.0790408335445747 1104 58-59 0.04242336888485454 1104 60-61 0.022088480882143102 1104 62-63 0.07419963226964299 1104 64-65 -0.05587491841307468 1104 66-67 0.22246296325630155 1104 68-69 -3.791324318989961 1104 70-71 0.4350508667015447 1104 72-73 0.7375039505347907 1104 74-75 0.39504424896263046 1104 76-77 0.39041443357671923 1104 78-79 -0.03602369550727502 1104 80-81 0.0202676569384046 1104 82-83 -0.04779404464159498 1104 84-85 0.04967698827885414 1104 86-87 0.1880489123074156 1104 88-89 0.14690406572272607 1104 90-91 0.24658963532537825 1104 92-93 0.018650437051352498 1104 94-95 0.3104387504979904 1104 96-97 0.014850831279492382 1104 98-99 0.3887018515434839 1104 100-101 0.3424633953427829 1105 1 0.03620912755590666 1105 2 0.008076650455429046 1105 3 0.06028652361451847 1105 4 -0.016121296371160554 1105 5 0.07358834714397489 1105 6 0.04362930514841423 1105 7 -0.03014804691961359 1105 8 -0.0415164993306405 1105 9 -0.0306819547678856 1105 10-11 -0.02280742217320153 1105 12-13 0.034007728429038764 1105 14-15 0.02990065879353665 1105 16-17 0.12959264201384713 1105 18-19 0.01387295552192569 1105 20-21 0.14857822217511085 1105 22-23 -0.05092818586415149 1105 24-25 -0.040348217099897 1105 26-27 -0.1145513068161037 1105 28-29 0.11490465866570787 1105 30-31 0.10832336086455285 1105 32-33 -0.14826968215105296 1105 34-35 -0.21143695306184185 1105 36-37 -0.16119972336237964 1105 38-39 0.004195708650662766 1105 40-41 -0.12786563420405628 1105 42-43 -0.0016132970646083322 1105 44-45 0.23487067250113824 1105 46-47 0.1522922891850058 1105 48-49 0.2861663110628072 1105 50-51 0.10390592899684492 1105 52-53 -0.04959868709411808 1105 54-55 -0.07800442316671763 1105 56-57 0.08312844064850822 1105 58-59 0.06643400862527216 1105 60-61 0.19569307646105827 1105 62-63 0.1822386081261449 1105 64-65 0.23027880903443076 1105 66-67 0.7218155571495863 1105 68-69 1.7823085378287367 1105 70-71 1.9608391338583786 1105 72-73 1.083302362517923 1105 74-75 0.664027104571435 1105 76-77 0.2572400754241748 1105 78-79 0.12776731011346953 1105 80-81 0.12101509596329763 1105 82-83 0.230714994617788 1105 84-85 0.17804259832213987 1105 86-87 0.24990929630415692 1105 88-89 0.20260496044892662 1105 90-91 0.046453716567995684 1105 92-93 -0.005283271850572646 1105 94-95 0.4780977564967692 1105 96-97 0.14645803748473085 1105 98-99 0.241384546305202 1105 100-101 0.05103454084525083 1106 1 0.337991606263639 1106 2 0.337396423709972 1106 3 0.37875929231664074 1106 4 0.3047445972209033 1106 5 0.33543585495507244 1106 6 0.4617506588083913 1106 7 0.3707710977908292 1106 8 0.42699711703998844 1106 9 0.40692455468578004 1106 10-11 0.45704851882909736 1106 12-13 0.4845539036150157 1106 14-15 0.694174681697092 1106 16-17 0.5471481905128783 1106 18-19 0.517651725703999 1106 20-21 0.6143917547334539 1106 22-23 0.611488206319649 1106 24-25 0.6882291293316314 1106 26-27 0.6557077414957675 1106 28-29 0.5682979019128496 1106 30-31 0.4531320504001357 1106 32-33 0.6782496923455739 1106 34-35 0.7517811407713637 1106 36-37 0.8012382233037485 1106 38-39 1.003729015618255 1106 40-41 0.7956344769362431 1106 42-43 0.641643421631116 1106 44-45 0.7889084332598557 1106 46-47 0.6327465114097919 1106 48-49 0.5688540684055212 1106 50-51 0.4619145738838597 1106 52-53 0.3869163861285756 1106 54-55 0.5704361966401166 1106 56-57 0.5194315774992688 1106 58-59 0.7580882381631895 1106 60-61 0.6249046442898454 1106 62-63 0.8370808610699179 1106 64-65 0.8652271889360321 1106 66-67 1.1242627606960731 1106 68-69 2.484843210234285 1106 70-71 2.9066736339242922 1106 72-73 1.888967775368048 1106 74-75 1.357235854921452 1106 76-77 0.8579774504153939 1106 78-79 0.9213282934212543 1106 80-81 0.8610110256302512 1106 82-83 0.9455614622326607 1106 84-85 0.8760111364759808 1106 86-87 0.7999558693820319 1106 88-89 1.0573888527073052 1106 90-91 0.8410431084910321 1106 92-93 1.0807636168465073 1106 94-95 1.2326225138404503 1106 96-97 0.8236914523209506 1106 98-99 1.162986371507973 1106 100-101 1.4120646622720443 1107 1 0.1434976454460113 1107 2 0.12112556236852257 1107 3 0.05873355768318689 1107 4 0.014774954094349368 1107 5 0.016255288735479212 1107 6 0.03651899425424432 1107 7 0.025266826783422403 1107 8 0.02935570868862669 1107 9 0.15132861725994928 1107 10-11 0.03409842460651191 1107 12-13 0.0855601777532442 1107 14-15 0.13717794609402745 1107 16-17 0.1433638156059729 1107 18-19 0.11504933732244638 1107 20-21 0.020839923663352522 1107 22-23 0.10279387860572342 1107 24-25 0.05762946816776804 1107 26-27 0.1179081968008262 1107 28-29 0.03153235518642106 1107 30-31 0.19758992699497924 1107 32-33 0.05884574989197233 1107 34-35 0.2663255810623113 1107 36-37 0.23764590679274988 1107 38-39 0.2761403930553783 1107 40-41 0.2073984620950995 1107 42-43 0.15307145354667284 1107 44-45 -0.026090495264547542 1107 46-47 0.04282952914631011 1107 48-49 0.2025725403564067 1107 50-51 0.18433536877460455 1107 52-53 0.25772549914859155 1107 54-55 0.1838060017853138 1107 56-57 0.24336132713914083 1107 58-59 0.04526075342089797 1107 60-61 0.20954713180450213 1107 62-63 0.14124178997679238 1107 64-65 -0.18734526539652308 1107 66-67 0.16403372438025698 1107 68-69 1.4801205494384675 1107 70-71 1.801238754174605 1107 72-73 0.8505481682296185 1107 74-75 0.27835675292370254 1107 76-77 -0.11343721448181654 1107 78-79 0.05507102972966749 1107 80-81 0.14549283985464356 1107 82-83 0.3525033267539399 1107 84-85 0.16885238223212085 1107 86-87 0.05060788390028392 1107 88-89 0.04488862447820097 1107 90-91 0.03768554603553653 1107 92-93 0.331947525301171 1107 94-95 0.5709426130888815 1107 96-97 0.2886568431789094 1107 98-99 0.45630209356240314 1107 100-101 0.548714076419941 1108 1 -0.1950466548327583 1108 2 -0.2313453890278474 1108 3 -0.22614351909231445 1108 4 -0.10819680608292259 1108 5 -0.09510497219233116 1108 6 -0.027063627148933733 1108 7 -0.05029660625984178 1108 8 -0.12157922085080486 1108 9 -0.12487557498722168 1108 10-11 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1.3792842117274837 2316 70-71 1.5221471529128792 2316 72-73 0.5816388641046935 2316 74-75 0.25141203215137864 2316 76-77 -0.1417197585302432 2316 78-79 -0.2976325578649508 2316 80-81 -0.4031932706069341 2316 82-83 -0.2321714628515501 2316 84-85 -0.5325523322077821 2316 86-87 -0.4304688304444966 2316 88-89 -0.3608778430122115 2316 90-91 -0.521503666123948 2316 92-93 -0.5484892533014083 2316 94-95 -0.4356033852650434 2316 96-97 -0.3781690147696253 2316 98-99 -0.5396289508513057 2316 100-101 -0.5064715740823118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 12.0 4 28.0 5 105.0 6 270.0 7 623.0 8 1308.0 9 2370.0 10 4168.0 11 6577.0 12 9332.0 13 11842.0 14 14354.0 15 18246.0 16 22678.0 17 27145.0 18 22060.0 19 14924.0 20 13603.0 21 13926.0 22 14635.0 23 15894.0 24 18320.0 25 21105.0 26 24966.0 27 30153.0 28 36805.0 29 43710.0 30 51509.0 31 61608.0 32 72515.0 33 87502.0 34 107927.0 35 137056.0 36 169282.0 37 210018.0 38 153946.0 39 16077.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.855767117869384 24.107780500131845 15.89741089478773 20.13904148721104 2 14.13322858934152 24.37831716965986 41.664440627942064 19.824013613056557 3 16.09008381853894 31.916745789335295 32.97537620168626 19.01779419043951 4 10.18804064259234 20.791020183990113 42.009474117808594 27.01146505560895 5 9.30179870932308 42.189756968282296 36.57167376081285 11.936770561581765 6 23.662295757242894 43.5091308526706 19.557119318961966 13.271454071124536 7 22.853631745159962 37.25676232321845 24.82967183852808 15.059934093093505 8 24.039612796924345 36.84450089248936 22.066456130715366 17.049430179870935 9 26.412261430729096 16.78896059316216 23.648496498695593 33.15028147741315 10-11 21.437285459288756 30.006933955787453 30.051180832074692 18.504599752849103 12-13 23.244198819167924 28.99258547301936 30.152581353837704 17.610634353975012 14-15 20.894720582177673 29.585850611011942 29.46014691747906 20.05928188933132 16-17 17.126896207036697 32.71684864395795 30.684673539330166 19.471581609675187 18-19 17.04905258821914 30.50120142798297 35.03295345324729 17.416792530550598 20-21 18.191816559110258 29.744610737333517 35.167376081285184 16.89619662227104 22-23 18.162192777701495 29.70458602224358 34.698510229301114 17.434710970753812 24-25 17.40575134268251 30.613470145180656 35.02261089016263 16.9581676219742 26-27 17.055667498515373 30.59220304889142 35.34122388172416 17.01090557086905 28-29 16.761367829660795 29.876743744591426 35.95573113954797 17.40615728619981 30-31 17.382521192440574 30.422098150306283 35.29765531540222 16.89772534185093 32-33 16.96501057107853 30.898203551703883 35.05976546932053 17.077020407897052 34-35 17.20635890535104 30.643065072019994 35.1251596248644 17.025416397764566 36-37 17.49552510938526 30.154113548996314 34.61398763696567 17.736373704652753 38-39 16.664851304672602 31.002935388804154 34.844710957147036 17.487502349376207 40-41 16.71404796914448 30.514807329972825 35.47872755344443 17.292417147438265 42-43 16.791652677832307 30.35223684468253 34.74709619289506 18.109014284590106 44-45 16.88907703705129 30.574084026175196 34.489574041417946 18.047264895355568 46-47 17.276386519540893 30.621814264877926 33.83480476286191 18.266994452719267 48-49 16.929121007307902 31.01242401846337 33.474962814530315 18.583492159698405 50-51 16.989688808870167 30.767962122435517 32.966125280456836 19.276223788237477 52-53 16.913174642106558 31.21578997630418 33.07088615769778 18.800149223891488 54-55 17.13311897857128 30.925340037157728 32.830111428525306 19.11142955574569 56-57 17.108211735603142 31.052490249632754 32.32156604736354 19.517731967400568 58-59 16.748353951991497 30.61457036584872 32.83355088380882 19.803524798350967 60-61 17.307470483064325 31.452873146523196 31.844990428795455 19.394665941617024 62-63 16.86452433661355 30.90427757721015 32.6221258917841 19.609072194392198 64-65 16.851645690247928 30.511978408758157 32.32177596340309 20.31459993759082 66-67 16.79500031627902 30.823418673663312 31.640862424127413 20.74071858593026 68-69 16.95924249541707 30.63735060883459 31.6372379095559 20.766168986192444 70-71 17.266870303083767 30.47037393380346 31.680648291604406 20.582107471508362 72-73 17.228574150089358 30.387157540075947 31.198769205798683 21.185499104036015 74-75 17.02725782808851 30.890282288815612 30.588493428392187 21.493966454703685 76-77 16.855527921603667 30.777648707421235 31.170021828265764 21.196801542709334 78-79 16.98782545412103 30.687682476583785 30.574089998609704 21.750402070685485 80-81 17.206842752960277 30.952626962863288 30.003278232706464 21.837252051469967 82-83 17.46867788206565 30.675646034236614 29.951390173750692 21.904285909947045 84-85 17.11880592670298 30.307999346420484 29.822929381831862 22.750265345044674 86-87 17.0929899267635 30.511557017882613 30.094656499031124 22.300796556322762 88-89 17.75343488967779 29.97932853081532 29.877893473623796 22.389343105883096 90-91 17.551067064536714 29.829166007306796 30.019851166028587 22.599915762127903 92-93 17.865426844945965 29.943493321735176 29.62503608689966 22.5660437464192 94-95 17.58676755868411 29.058247654473586 30.012810176137343 23.342174610704966 96-97 18.0807399358324 29.011616429514348 29.660776912024474 23.246866722628777 98-99 17.869680682136156 29.110163890524493 29.59515761609413 23.42499781124522 100-101 17.679755267189883 28.95302295897787 29.89761717826036 23.46960459557189 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4073.0 1 2647.0 2 1438.0 3 1723.0 4 1500.0 5 1410.5 6 2418.0 7 4567.5 8 6420.5 9 6805.5 10 5755.5 11 4588.5 12 4340.5 13 4335.0 14 4406.5 15 4502.5 16 4609.5 17 4841.0 18 5205.5 19 5619.0 20 6134.5 21 6901.0 22 7757.5 23 8702.5 24 9716.5 25 11455.0 26 14809.5 27 18582.0 28 20411.0 29 23007.5 30 28497.5 31 33788.5 32 41460.5 33 51826.5 34 61087.0 35 68041.5 36 76071.5 37 81404.5 38 78161.0 39 73506.0 40 68719.5 41 62916.0 42 57173.5 43 55453.5 44 55652.5 45 53408.5 46 48718.0 47 42306.0 48 38167.0 49 33827.5 50 28773.5 51 23687.5 52 18602.5 53 14348.5 54 10986.5 55 8772.0 56 7073.0 57 5682.5 58 4680.5 59 3990.0 60 3412.5 61 2801.5 62 2120.5 63 1298.0 64 714.5 65 504.5 66 412.5 67 343.0 68 259.5 69 182.5 70 132.5 71 110.5 72 113.0 73 120.5 74 164.0 75 172.0 76 102.5 77 59.5 78 42.5 79 35.5 80 22.0 81 14.0 82 13.5 83 8.0 84 5.0 85 2.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02361664149388988 2 0.0240972126870795 3 6.86530275985171E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.492242207881368E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.86530275985171E-5 26-27 3.4326513799258544E-4 28-29 3.432651379925855E-5 30-31 5.148977069888782E-4 32-33 0.0021625703693532883 34-35 0.0041191816559110256 36-37 0.10548537690512153 38-39 0.09793354386928464 40-41 0.10713304956748593 42-43 0.1078195798434711 44-45 0.10174378690100234 46-47 0.09491281065494989 48-49 0.09573664698613209 50-51 0.09508444322394617 52-53 0.07339008650281477 54-55 0.06271454071124537 56-57 0.03439516682685707 58-59 0.015996155430454483 60-61 0.01016064808458053 62-63 0.0027117945901414253 64-65 0.005732527804476177 66-67 0.1502128243855554 68-69 0.400693395578745 70-71 0.3877522998764245 72-73 0.17709048469037486 74-75 0.030859535905533433 76-77 0.031614719209117126 78-79 0.005320609638885075 80-81 0.0015446931209666347 82-83 1.0297954139777564E-4 84-85 4.1191816559110257E-4 86-87 0.0012014279829740493 88-89 2.0595908279555128E-4 90-91 7.208567897844296E-4 92-93 0.003192365783331045 94-95 0.07665110531374433 96-97 0.017678154606618153 98-99 0.02008101057256625 100-101 0.01060689276397089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1456600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.146906606712086 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06588350898079 22.32857272888861 2 11.861242940392307 7.151595663270599 3 4.680538063348008 4.233112315947404 4 2.4575211399514605 2.9634664116054923 5 1.4595444012367125 2.200037437621634 6 0.9108012954053288 1.64747049539141 7 0.6427200721261407 1.3563215392052215 8 0.4653445478135657 1.1222958898302584 9 0.361357793720572 0.9804437693011455 >10 2.31150953274666 13.882848970815687 >50 0.3725709795059274 7.870648125032975 >100 0.3750516848429498 22.415532620627918 >500 0.027958398173066515 5.661889669638443 >1k 0.007046425555813513 3.053137600372052 >5k 4.5460810037506526E-4 1.0920166266306592 >10k+ 4.5460810037506526E-4 2.040610135820432 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16280 1.1176712893038583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13499 0.9267472195523824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9959 0.6837155018536317 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5977 0.4103391459563367 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3334 0.228889194013456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2414 0.16572840862282026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.1511053137443361 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1992 0.13675683097624605 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1865 0.1280378964712344 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.12117259371138267 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1579 0.10840313057805849 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1504 0.10325415350816972 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1488 0.10215570506659344 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1469 0.10085129754222161 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.86530275985171E-5 0.0 10-11 0.0 0.0 0.0 1.373060551970342E-4 0.0 12-13 0.0 0.0 0.0 1.373060551970342E-4 0.0 14-15 0.0 0.0 0.0 1.7163256899629275E-4 0.0 16-17 0.0 0.0 0.0 2.0595908279555128E-4 0.0 18-19 0.0 0.0 0.0 3.0893862419332694E-4 0.0 20-21 0.0 0.0 0.0 5.835507345873953E-4 0.0 22-23 0.0 0.0 0.0 0.00212824385555403 0.0 24-25 0.0 0.0 0.0 0.006556364135658382 0.0 26-27 0.0 0.0 0.0 0.014794727447480433 0.0 28-29 0.0 0.0 0.0 0.03669504325140739 0.0 30-31 0.0 0.0 0.0 0.11097761911300288 0.0 32-33 0.0 0.0 0.0 0.23911849512563504 0.0 34-35 0.0 0.0 0.0 0.4415076204860634 0.0 36-37 0.0 0.0 0.0 0.7462240834820816 0.0 38-39 0.0 0.0 0.0 1.1902718659892901 0.0 40-41 0.0 0.0 0.0 1.6989221474667033 0.0 42-43 0.0 0.0 0.0 2.1765412604695866 0.0 44-45 0.0 0.0 0.0 2.7126184264726074 0.0 46-47 0.0 0.0 0.0 3.332452286145819 0.0 48-49 0.0 0.0 0.0 3.9774818069476865 0.0 50-51 0.0 0.0 0.0 4.67636962790059 0.0 52-53 0.0 0.0 0.0 5.314945764108197 0.0 54-55 0.0 0.0 0.0 5.947720719483729 0.0 56-57 0.0 0.0 0.0 6.647638335850611 0.0 58-59 0.0 0.0 0.0 7.36942194150762 0.0 60-61 0.0 0.0 0.0 8.104489908004943 0.0 62-63 0.0 0.0 0.0 8.778559659480983 0.0 64-65 0.0 0.0 0.0 9.455478511602362 0.0 66-67 0.0 0.0 0.0 10.14674584649183 0.0 68-69 0.0 0.0 0.0 10.908485514211176 0.0 70-71 0.0 0.0 0.0 11.802244954002472 0.0 72-73 0.0 0.0 0.0 12.64671151997803 0.0 74-75 0.0 0.0 0.0 13.479953315941234 0.0 76-77 0.0 0.0 0.0 14.354970479198133 0.0 78-79 0.0 0.0 0.0 15.310620623369491 0.0 80-81 0.0 0.0 0.0 16.304201565289027 0.0 82-83 0.0 0.0 0.0 17.265549910751062 0.0 84-85 0.0 0.0 0.0 18.18011121790471 0.0 86-87 0.0 0.0 0.0 19.094947137168752 0.0 88-89 0.0 0.0 0.0 20.03515035013044 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGCG 30 1.4128764E-6 47.882153 64-65 GGTATCA 7475 0.0 45.954803 1 GTATCAA 12440 0.0 36.95787 1 TACTCGC 150 9.713403E-10 31.61901 7 CATGGGT 1155 0.0 29.976463 4 GTCGCGT 50 4.692217E-5 28.729294 64-65 GTACATG 10955 0.0 28.671417 1 GTATGGT 315 0.0 28.618483 1 TCAACGC 16235 0.0 27.957556 4 TCGCGTA 60 5.06001E-6 27.931255 66-67 TATCAAC 16285 0.0 27.906588 2 ATCAACG 16325 0.0 27.832476 3 CAACGCA 16410 0.0 27.68831 5 CATGGGG 3255 0.0 27.684849 4 ACATGGG 10680 0.0 27.666634 3 AACGCAG 16555 0.0 27.474445 6 GGATGAC 485 0.0 27.38141 8 TACATGG 11380 0.0 27.137367 2 GTACACG 70 0.00843861 27.112247 1 GACCGGT 105 6.9782596E-5 27.102009 6 >>END_MODULE