FastQCFastQC Report
Fri 27 May 2016
SRR939869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4213656
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126450.3000956888744596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89160.2115977194151587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61580.14614387126049208No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA51040.1211299640976862No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA50560.11999081083030982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159850.036.4486161
GGTATCA95050.035.340811
ATCAACG199800.029.8748873
TATCAAC200750.029.7557372
TCAACGC201900.029.611514
CAACGCA201400.029.4729865
AACGCAG207650.028.7229926
TACATGG157600.027.8173832
GTACATG157200.027.3747441
ACATGGG159650.026.2726883
ACGCAGA229700.025.593917
CGCAGAG231950.025.2445568
GCAGAGT244550.022.7017849
CATGGGG64150.022.263644
GAGTACT130850.021.90098812-13
CATGGGA87650.021.7620834
AGTACTT144650.020.68079212-13
AGAGTAC211250.020.62922510-11
GTACTTT139250.020.42822514-15
TAAGGTG25000.019.359165
Produced by FastQC (version 0.11.5)