##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939869_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4213656 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.088051801096245 31.0 30.0 33.0 16.0 34.0 2 29.971841317848444 31.0 30.0 33.0 25.0 34.0 3 30.245945800986128 31.0 30.0 34.0 26.0 34.0 4 34.04641598649724 35.0 35.0 37.0 30.0 37.0 5 34.026127666805266 35.0 35.0 37.0 30.0 37.0 6 34.06901085423205 36.0 35.0 37.0 30.0 37.0 7 34.002983869589734 36.0 35.0 37.0 29.0 37.0 8 33.86752739188961 35.0 35.0 37.0 28.0 37.0 9 35.17793906289455 38.0 35.0 39.0 28.0 39.0 10-11 35.03075096780563 37.0 34.5 39.0 28.0 39.0 12-13 34.94394381031579 37.0 34.0 39.0 27.0 39.0 14-15 35.661790853358696 38.0 34.0 40.0 27.0 41.0 16-17 35.51158186619885 38.0 34.0 40.0 27.0 41.0 18-19 35.388005926444876 38.0 34.0 40.0 27.0 41.0 20-21 35.56213191110048 38.0 34.0 40.0 27.0 41.0 22-23 35.468527331134766 38.0 34.0 40.0 27.0 41.0 24-25 35.27663114881708 38.0 34.0 40.0 26.5 41.0 26-27 35.176411766883675 38.0 34.0 40.0 25.5 41.0 28-29 35.239478020987 38.0 34.0 40.0 26.0 41.0 30-31 35.012230234266866 38.0 34.0 40.0 25.0 41.0 32-33 34.6706431422024 38.0 33.5 40.0 24.0 41.0 34-35 34.498907480819504 38.0 33.0 40.0 24.0 41.0 36-37 34.30082973076112 38.0 33.0 40.0 24.0 41.0 38-39 34.08746751039952 38.0 33.0 40.0 23.5 41.0 40-41 33.877302869527085 38.0 33.0 40.0 22.5 41.0 42-43 33.502152525028144 38.0 32.0 40.0 19.0 41.0 44-45 33.1758687704929 37.0 31.5 40.0 18.0 41.0 46-47 32.806529056951966 37.0 31.0 40.0 15.5 41.0 48-49 32.46125538012595 37.0 31.0 40.0 14.0 41.0 50-51 31.844843765129383 36.5 30.0 38.5 10.0 40.5 52-53 32.00878749950162 36.5 30.5 39.0 10.0 40.0 54-55 32.139526933380424 37.0 31.0 39.0 9.0 40.5 56-57 31.864090234228897 36.5 30.5 39.0 8.0 40.5 58-59 31.49151651202661 36.0 30.0 39.0 7.0 40.0 60-61 31.124083456266955 36.0 29.0 39.0 4.0 40.0 62-63 30.699176202328808 35.0 28.5 39.0 2.0 40.0 64-65 30.183298541693958 35.0 28.0 38.0 2.0 40.0 66-67 29.609449371282324 34.0 27.5 38.0 2.0 40.0 68-69 29.092657540150405 34.0 26.5 37.5 2.0 39.5 70-71 28.517932764326275 34.0 26.0 37.0 2.0 39.0 72-73 27.845585994680157 33.0 25.5 36.0 2.0 39.0 74-75 27.50469805793354 33.0 25.0 36.0 2.0 39.0 76-77 26.934613670408787 32.0 24.0 35.5 2.0 38.0 78-79 26.261982587093016 32.0 22.0 35.0 2.0 37.0 80-81 25.529859343050312 31.0 20.0 35.0 2.0 36.5 82-83 24.780544733599516 31.0 17.5 34.0 2.0 36.0 84-85 24.034425093078315 30.5 10.5 34.0 2.0 35.5 86-87 23.216488958756955 30.0 4.5 34.0 2.0 35.0 88-89 22.270229107454426 29.0 2.0 34.0 2.0 35.0 90-91 20.848380598700984 27.5 2.0 33.5 2.0 35.0 92-93 19.250014476739437 25.5 2.0 32.5 2.0 34.0 94-95 18.71330478805104 25.0 2.0 32.0 2.0 34.0 96-97 18.349769416392796 25.0 2.0 32.0 2.0 34.0 98-99 17.402749180284296 22.0 2.0 32.0 2.0 34.0 100-101 15.675800183973253 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6461768837580344 1101 2 0.8945667374557722 1101 3 0.9971099138399069 1101 4 0.9900846200064279 1101 5 0.9717098962955291 1101 6 0.9899858923963833 1101 7 0.955071497555096 1101 8 0.9752880001812159 1101 9 0.9106508529006021 1101 10-11 1.083646206601827 1101 12-13 1.1980550519707123 1101 14-15 1.326782890051824 1101 16-17 1.1313415241076825 1101 18-19 1.1932711953171093 1101 20-21 1.1412269871444423 1101 22-23 1.340675231545319 1101 24-25 1.4972575019678445 1101 26-27 1.3801528999140658 1101 28-29 1.3538346919964255 1101 30-31 1.247881929615481 1101 32-33 1.3036658786568793 1101 34-35 1.2704364629523255 1101 36-37 1.2236837581577262 1101 38-39 1.2385694838734693 1101 40-41 1.2541717158889085 1101 42-43 1.3310985655167755 1101 44-45 1.212871408863542 1101 46-47 0.9505949740548942 1101 48-49 0.8937714586301055 1101 50-51 0.8630160255464965 1101 52-53 0.8086499815249297 1101 54-55 0.7475197338909325 1101 56-57 0.7187698250750998 1101 58-59 0.6934074359165443 1101 60-61 0.6869304805778569 1101 62-63 0.015171058630880196 1101 64-65 0.36143128003287117 1101 66-67 0.5935717256182578 1101 68-69 0.3730234982146037 1101 70-71 0.44645309040982184 1101 72-73 0.35713234547620587 1101 74-75 0.3279979519637095 1101 76-77 0.002408549079603972 1101 78-79 -0.0650128972879962 1101 80-81 -0.2222655542599945 1101 82-83 -0.05237034721729117 1101 84-85 -0.08707378885992867 1101 86-87 -0.5041756417768788 1101 88-89 -4.539202155092603 1101 90-91 -8.256961796226205 1101 92-93 -10.810891788855573 1101 94-95 -8.68909010715292 1101 96-97 -5.132613153761035 1101 98-99 -3.6968247733170987 1101 100-101 -2.5572960280564523 1102 1 1.7260188664201586 1102 2 1.089372556510483 1102 3 0.920080253469898 1102 4 0.9620014345085863 1102 5 0.916947400186352 1102 6 0.8621939094498714 1102 7 0.8425729181029453 1102 8 1.0276412248276259 1102 9 1.067692794387746 1102 10-11 1.1246228922191719 1102 12-13 1.1231153116385002 1102 14-15 1.3713563637979291 1102 16-17 1.3644361682268524 1102 18-19 1.3591196970531598 1102 20-21 1.2808573893108814 1102 22-23 1.3272284566155221 1102 24-25 1.4141940123171821 1102 26-27 1.3736824508224288 1102 28-29 1.2170425416511037 1102 30-31 1.3039145823088987 1102 32-33 1.494777882958168 1102 34-35 1.450770314839069 1102 36-37 1.387149419821867 1102 38-39 1.3234629848851682 1102 40-41 1.3078577740886033 1102 42-43 1.3299603428371043 1102 44-45 1.2651208596315158 1102 46-47 1.15583176864763 1102 48-49 1.1246440257571848 1102 50-51 1.1624944461002649 1102 52-53 1.2572263723151664 1102 54-55 1.2900453882323504 1102 56-57 1.2722626664618915 1102 58-59 1.1152944230959854 1102 60-61 1.1905521945119233 1102 62-63 1.3074479288999292 1102 64-65 1.2005850026600697 1102 66-67 1.1779074350629948 1102 68-69 1.1054956105879405 1102 70-71 1.1115993201280538 1102 72-73 1.0524678518341801 1102 74-75 0.9408470540380591 1102 76-77 1.0309894142470526 1102 78-79 0.9000278522214913 1102 80-81 0.8615413043178997 1102 82-83 0.6178939254270794 1102 84-85 0.5652093870244705 1102 86-87 0.609520937677722 1102 88-89 0.04248863086878174 1102 90-91 -5.835435368906007 1102 92-93 -10.943000954737755 1102 94-95 -8.500595312408283 1102 96-97 -4.740920796414432 1102 98-99 -3.0683948681946394 1102 100-101 -2.109003133450006 1103 1 2.0174150713480152 1103 2 1.2008953649022729 1103 3 1.023934136393276 1103 4 0.9246179352914297 1103 5 1.0158287826927221 1103 6 0.830894897543935 1103 7 0.7323336163449312 1103 8 0.8082369356214301 1103 9 0.9454410473146311 1103 10-11 0.8178447633608528 1103 12-13 0.8551180766153053 1103 14-15 0.957223187787406 1103 16-17 0.9855062818470302 1103 18-19 0.9185426967891104 1103 20-21 0.8267831891416719 1103 22-23 0.8632993947094718 1103 24-25 0.7995419933052688 1103 26-27 0.9504859584263698 1103 28-29 0.7582417234542191 1103 30-31 0.8313165590584575 1103 32-33 0.9263042553673273 1103 34-35 0.9347868085814568 1103 36-37 0.8951239929047503 1103 38-39 0.9381791483495974 1103 40-41 0.8443905803126768 1103 42-43 0.9187650388959767 1103 44-45 0.9249937351518085 1103 46-47 0.9023974578164697 1103 48-49 0.8721707869069988 1103 50-51 0.8121592022073294 1103 52-53 0.9513599368417616 1103 54-55 0.8368643539475542 1103 56-57 0.7806206184454538 1103 58-59 0.8721371202935693 1103 60-61 0.8843894874621867 1103 62-63 0.8695701608843329 1103 64-65 0.825360560238181 1103 66-67 0.7158677290573756 1103 68-69 0.7557950739473078 1103 70-71 0.6894226824191207 1103 72-73 0.8276778428056737 1103 74-75 0.6175316150887262 1103 76-77 0.49857176238990775 1103 78-79 0.30621014789221235 1103 80-81 0.3243862267244495 1103 82-83 -0.09344831843626267 1103 84-85 -0.1990322675015328 1103 86-87 -0.05303864999219243 1103 88-89 -0.5580842562901474 1103 90-91 -6.5962245359999745 1103 92-93 -10.328514167344023 1103 94-95 -9.00181265340766 1103 96-97 -5.501954863378769 1103 98-99 -3.9322560576904912 1103 100-101 -2.950544748377691 1104 1 1.941124318121723 1104 2 1.1711014808464135 1104 3 0.9533658047178442 1104 4 0.6888353871185302 1104 5 0.699338087610478 1104 6 0.7828224705114977 1104 7 0.7036591634404061 1104 8 0.7911838585093989 1104 9 0.8402510629383215 1104 10-11 0.888449542970271 1104 12-13 0.6689190340641602 1104 14-15 0.9372537963787053 1104 16-17 0.894667983202055 1104 18-19 0.8236022790900321 1104 20-21 0.8232256549748556 1104 22-23 0.7670893899626066 1104 24-25 0.7906324636955944 1104 26-27 0.8155304810164594 1104 28-29 0.7194525132704044 1104 30-31 0.7761852462643049 1104 32-33 0.8101183369498912 1104 34-35 0.8025062738212085 1104 36-37 0.8056584385522925 1104 38-39 0.8252305737947268 1104 40-41 0.777368033141137 1104 42-43 0.7647026672730348 1104 44-45 0.6746233090438025 1104 46-47 0.7503897185772672 1104 48-49 0.701305390182263 1104 50-51 0.49777363908051697 1104 52-53 0.4969849810228908 1104 54-55 0.45218657622412906 1104 56-57 0.5462863481124742 1104 58-59 0.41423731355395077 1104 60-61 0.582764897537416 1104 62-63 0.588730988448102 1104 64-65 0.44927497530974847 1104 66-67 0.5443264629463087 1104 68-69 0.48829932228971984 1104 70-71 0.3187434770876685 1104 72-73 0.4299221147490826 1104 74-75 0.4105456443780966 1104 76-77 0.41926012286688064 1104 78-79 0.23739093338547335 1104 80-81 0.1716854012458846 1104 82-83 -0.040128324087614686 1104 84-85 -0.06595846264128014 1104 86-87 0.25475758226977163 1104 88-89 0.07065325154016122 1104 90-91 -2.9021337484167127 1104 92-93 -7.936504216290722 1104 94-95 -5.599743720005613 1104 96-97 -2.7835363747230453 1104 98-99 -1.7958555875505073 1104 100-101 -1.121833900292776 1105 1 1.8762917884510024 1105 2 1.1577846439760577 1105 3 0.9029163111259848 1105 4 0.8531535260186303 1105 5 0.7981774978123042 1105 6 0.7557670453729557 1105 7 0.7156208503175776 1105 8 0.6869708393210203 1105 9 0.7808550401269727 1105 10-11 0.7035982402546281 1105 12-13 0.6410563709127715 1105 14-15 0.7766834075297524 1105 16-17 0.6796054895684591 1105 18-19 0.6244221550513416 1105 20-21 0.6458973610599514 1105 22-23 0.6708380594026195 1105 24-25 0.6619480128378044 1105 26-27 0.4853256966335806 1105 28-29 0.6355564555806481 1105 30-31 0.853173920852889 1105 32-33 0.9034338983506203 1105 34-35 0.7857118121029529 1105 36-37 0.7286310912576752 1105 38-39 0.6718868002901104 1105 40-41 0.5749199924755501 1105 42-43 0.6685740678291694 1105 44-45 0.6287779835232286 1105 46-47 0.7198066129750913 1105 48-49 0.44946734213529993 1105 50-51 0.5059129302871632 1105 52-53 0.4848690411258261 1105 54-55 0.5886698525407539 1105 56-57 0.5447327268732494 1105 58-59 0.4769332127001995 1105 60-61 0.493373010516585 1105 62-63 0.5398958979145654 1105 64-65 0.6053204386510842 1105 66-67 0.554714754427021 1105 68-69 0.503298640774485 1105 70-71 0.2878783526009592 1105 72-73 0.19990736658428432 1105 74-75 0.2906041437778626 1105 76-77 0.18695164543028753 1105 78-79 0.08633067372087666 1105 80-81 -0.023398407615797367 1105 82-83 -0.1473702917665669 1105 84-85 -0.17340918996542953 1105 86-87 0.10583657995495699 1105 88-89 -0.1924240334549161 1105 90-91 -3.3372996864312228 1105 92-93 -8.369843562135333 1105 94-95 -5.7815954071133575 1105 96-97 -2.9836338445357136 1105 98-99 -1.9134255026952935 1105 100-101 -1.24384285835084 1106 1 1.6088970644431981 1106 2 0.9978353931205781 1106 3 0.6074305164891207 1106 4 0.7047978762536857 1106 5 0.6866725927945581 1106 6 0.7444502759687524 1106 7 0.7407967433745952 1106 8 -0.3462952848754668 1106 9 -0.41585391083084744 1106 10-11 -0.5430379799235041 1106 12-13 -0.21576255817834067 1106 14-15 0.5761928058155874 1106 16-17 0.656849750058079 1106 18-19 0.735518633392509 1106 20-21 0.6626513996045134 1106 22-23 0.6188205860264304 1106 24-25 0.6309620273169969 1106 26-27 0.7415489628876983 1106 28-29 0.6197275295676619 1106 30-31 0.5993794029197943 1106 32-33 0.6923381695537287 1106 34-35 0.7383048105598888 1106 36-37 0.7062100232670119 1106 38-39 0.7924100221712038 1106 40-41 0.5466803371859612 1106 42-43 0.8926969011465147 1106 44-45 0.7306194888879745 1106 46-47 0.7393017599257519 1106 48-49 0.7006618908496804 1106 50-51 0.6567232126354945 1106 52-53 0.7471273880508917 1106 54-55 0.8109578014678078 1106 56-57 0.721197586238759 1106 58-59 0.5497732509569424 1106 60-61 0.24922033034613378 1106 62-63 0.50502669838788 1106 64-65 0.4214198502088564 1106 66-67 0.5015649694962541 1106 68-69 0.5686543794853485 1106 70-71 0.6406272562675319 1106 72-73 0.5629569074634304 1106 74-75 0.3111590713818799 1106 76-77 0.2947758633058193 1106 78-79 0.21828889312347854 1106 80-81 0.20915794811991262 1106 82-83 -0.13711755043329887 1106 84-85 -0.1930233645472832 1106 86-87 0.15171678479041617 1106 88-89 -0.10178851485022733 1106 90-91 -3.565468110029677 1106 92-93 -8.837155136697998 1106 94-95 -6.136239158712385 1106 96-97 -3.288963654011553 1106 98-99 -2.138422629442232 1106 100-101 -1.6340523359884358 1107 1 1.8382498679946302 1107 2 1.132341372163932 1107 3 0.8394061503876991 1107 4 0.858963959680338 1107 5 0.6984632062963954 1107 6 0.6806942269365948 1107 7 0.6691642045808024 1107 8 0.6724659633248251 1107 9 0.7472692394651119 1107 10-11 0.6254581856900003 1107 12-13 0.6304886666256948 1107 14-15 0.7161079193974231 1107 16-17 0.7928261188390735 1107 18-19 0.7916557764496233 1107 20-21 0.5894822316713828 1107 22-23 0.5497781328189433 1107 24-25 0.5092153817802867 1107 26-27 0.6559819036171817 1107 28-29 0.45513311467404804 1107 30-31 0.7220256545195411 1107 32-33 0.8767462892545765 1107 34-35 0.7471342728459192 1107 36-37 0.44122292120334095 1107 38-39 0.43363392634653763 1107 40-41 0.5522053281874051 1107 42-43 0.5908646942996043 1107 44-45 0.4934605593167021 1107 46-47 0.35278660235096027 1107 48-49 0.6019920530237286 1107 50-51 0.5961487795447873 1107 52-53 0.6426890981772644 1107 54-55 0.6065060137538367 1107 56-57 0.5621997758884163 1107 58-59 0.6297000876630641 1107 60-61 0.2718570834656333 1107 62-63 0.3606457993317278 1107 64-65 0.5080639871469472 1107 66-67 0.5708353862295787 1107 68-69 0.6566436761978736 1107 70-71 0.5652901851989007 1107 72-73 0.5402739117427444 1107 74-75 0.40936248383588847 1107 76-77 0.29028345709081194 1107 78-79 0.1245881352447391 1107 80-81 0.11917709505640417 1107 82-83 -0.12479064773772919 1107 84-85 0.11270921823946978 1107 86-87 0.6180066784074363 1107 88-89 0.32088298271016313 1107 90-91 -3.0848767546465368 1107 92-93 -4.583374241641206 1107 94-95 -2.7255077507376626 1107 96-97 -1.1598375641859278 1107 98-99 -0.6393541054060776 1107 100-101 -0.39227464541892765 1108 1 1.5979159577839006 1108 2 0.9222788205560271 1108 3 -0.4140240078576909 1108 4 -0.24500970756320584 1108 5 0.3487601284858144 1108 6 0.479517849500418 1108 7 0.5241078176431202 1108 8 0.4587475926075726 1108 9 0.613938168396075 1108 10-11 0.5253835309680923 1108 12-13 0.535035817869371 1108 14-15 0.5538526681164129 1108 16-17 0.5913183196829053 1108 18-19 0.5168732366054982 1108 20-21 0.3031293410631761 1108 22-23 0.34600015143738005 1108 24-25 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1.0189413309314794 2311 82-83 1.2525584608717253 2311 84-85 1.5032226622009475 2311 86-87 1.8507643288710192 2311 88-89 2.2137471806937334 2311 90-91 2.888160855725392 2311 92-93 3.7043169905660065 2311 94-95 3.363542968401685 2311 96-97 2.702778167087164 2311 98-99 2.306772828306361 2311 100-101 2.2990974218681046 2312 1 1.3169362423181639 2312 2 0.6851982537083039 2312 3 0.05986808763311657 2312 4 -0.5222881873864864 2312 5 -0.15718608901953957 2312 6 -0.2035073765040636 2312 7 0.09196125824743717 2312 8 0.2590834352066125 2312 9 0.3247951938781384 2312 10-11 0.19839733614305288 2312 12-13 0.19324860113178488 2312 14-15 0.3134631792092364 2312 16-17 0.03690333740043883 2312 18-19 -0.4042874383730535 2312 20-21 0.3961440502425617 2312 22-23 0.4255913413509944 2312 24-25 0.3549928541974481 2312 26-27 0.4234731926603388 2312 28-29 0.3733679022873133 2312 30-31 0.404154920211532 2312 32-33 0.5705939668532451 2312 34-35 0.44498118307733847 2312 36-37 0.39688426240070385 2312 38-39 0.3402997602115292 2312 40-41 0.3413421738618112 2312 42-43 0.4923998749655212 2312 44-45 0.31771427234811966 2312 46-47 0.49908873982569446 2312 48-49 0.5084004619412568 2312 50-51 0.38191143998923494 2312 52-53 0.33742727871379685 2312 54-55 0.5074353311985149 2312 56-57 0.4344273270499457 2312 58-59 0.4998481627758302 2312 60-61 0.5564597701433343 2312 62-63 0.6993853539910297 2312 64-65 0.6957499733254124 2312 66-67 0.6965114055565245 2312 68-69 0.5675630446367741 2312 70-71 0.577515862556659 2312 72-73 0.629823440391867 2312 74-75 0.6527022267678575 2312 76-77 0.9139683368327631 2312 78-79 0.9475690001390227 2312 80-81 1.1069134954793505 2312 82-83 1.165581123854512 2312 84-85 1.3482258304022423 2312 86-87 1.6298955172290839 2312 88-89 1.9201174000241075 2312 90-91 2.904401518598668 2312 92-93 4.046194828104447 2312 94-95 3.589941761404141 2312 96-97 2.6977859172522614 2312 98-99 2.438734882427582 2312 100-101 2.445491869707574 2313 1 -7.397541493868271 2313 2 -2.731888998722969 2313 3 -1.5869436636921321 2313 4 -0.8392155836795325 2313 5 -0.7211358081117112 2313 6 -0.254558115994044 2313 7 -0.12438331863351237 2313 8 -0.09533423371877348 2313 9 0.004894583683821452 2313 10-11 -0.18888019462658434 2313 12-13 -0.32184430901305916 2313 14-15 -0.29884818512168465 2313 16-17 -0.533383413846046 2313 18-19 -0.6237402877260152 2313 20-21 -0.46314848263970276 2313 22-23 -1.109411735700256 2313 24-25 -0.3928162564297253 2313 26-27 -0.4931789318177593 2313 28-29 -0.5317723660917721 2313 30-31 -0.28705688225787895 2313 32-33 -0.40097378638474623 2313 34-35 -0.20178488506376624 2313 36-37 -0.07941557296479829 2313 38-39 -0.1449744579146497 2313 40-41 -0.11838998090370723 2313 42-43 -0.09608425196582004 2313 44-45 -0.2482569495212843 2313 46-47 -1.065052222902775 2313 48-49 -0.5139530551913438 2313 50-51 -0.4098317226733563 2313 52-53 -0.8070894614602011 2313 54-55 -0.4696042960757154 2313 56-57 -0.17989910765582806 2313 58-59 -0.2903697328851784 2313 60-61 -0.17296675974449727 2313 62-63 -0.21716803092287762 2313 64-65 -0.1488252010670763 2313 66-67 -0.014416279013165223 2313 68-69 -0.05088693192653082 2313 70-71 -0.0019010274791888548 2313 72-73 -0.03057080986306815 2313 74-75 0.08508492250279431 2313 76-77 0.20903269761299725 2313 78-79 0.09630992520074955 2313 80-81 0.2913578199300737 2313 82-83 0.5107697393949842 2313 84-85 0.6368396511932382 2313 86-87 1.1780575610842412 2313 88-89 1.7041072861872166 2313 90-91 2.336977558346188 2313 92-93 3.2389009389041945 2313 94-95 2.966088194912704 2313 96-97 2.2110784548603775 2313 98-99 1.9240790474597453 2313 100-101 1.5259898846769602 2314 1 0.5858779377277656 2314 2 0.03161981516277734 2314 3 -1.2154318973613094 2314 4 -0.24602007232861922 2314 5 -0.07887532601325375 2314 6 -3.364648233039169 2314 7 -2.6761384479681247 2314 8 -2.3756670920515717 2314 9 -3.169230085941173 2314 10-11 -2.4665885984622875 2314 12-13 -1.128583588119092 2314 14-15 -1.1602490281122257 2314 16-17 -2.007582239014525 2314 18-19 -3.0691149758965253 2314 20-21 -2.7398484964870136 2314 22-23 -3.914441648723585 2314 24-25 -3.0262280137174002 2314 26-27 -1.7978218618810544 2314 28-29 -1.7491910855402466 2314 30-31 -1.9567133646728578 2314 32-33 -2.1121199288834873 2314 34-35 -1.519999507120417 2314 36-37 -1.9223034054857848 2314 38-39 -3.2351738980875453 2314 40-41 -2.637148057031439 2314 42-43 -1.6961805473148885 2314 44-45 -2.064217629862405 2314 46-47 -1.6355905531145751 2314 48-49 -2.271640786966053 2314 50-51 -3.2394207912790236 2314 52-53 -2.016520895003339 2314 54-55 -2.4041741056115704 2314 56-57 -1.513932828337147 2314 58-59 -0.8479674482903867 2314 60-61 -1.3401785024783663 2314 62-63 -0.9735311276202232 2314 64-65 -1.6705191196236768 2314 66-67 -1.066525495106852 2314 68-69 -1.0571188412238008 2314 70-71 -1.1208978461295445 2314 72-73 -2.0754085083971603 2314 74-75 -2.2121255414740517 2314 76-77 -1.2618976581711152 2314 78-79 -2.289067921005053 2314 80-81 -1.034274564273531 2314 82-83 -0.6401115785301492 2314 84-85 -1.931168541682883 2314 86-87 -0.44552057238266585 2314 88-89 0.6629294114389914 2314 90-91 1.7857205084033119 2314 92-93 2.8074576814508454 2314 94-95 2.652521298258172 2314 96-97 1.6304847053681684 2314 98-99 1.3343256267847217 2314 100-101 1.3987421689538273 2315 1 -2.602196573437812 2315 2 -1.8031597360404987 2315 3 -2.5611412143161054 2315 4 -3.956813897605233 2315 5 -3.6777863697050535 2315 6 -2.863973613760759 2315 7 -5.706519420479019 2315 8 -2.9147327653765096 2315 9 -2.484968590034036 2315 10-11 -3.7119642748490946 2315 12-13 -2.8126783353676856 2315 14-15 -1.888899677727288 2315 16-17 -2.3994917244138847 2315 18-19 -1.896891383805567 2315 20-21 -1.2147624003908035 2315 22-23 -0.8064879849341651 2315 24-25 -2.2372074644232427 2315 26-27 -3.7736561758668614 2315 28-29 -3.113176420530607 2315 30-31 -3.198297467224961 2315 32-33 -3.9343101989687206 2315 34-35 -3.522886590206028 2315 36-37 -2.7411136476842266 2315 38-39 -1.6567332912471926 2315 40-41 -1.335087725670867 2315 42-43 -2.773672763939441 2315 44-45 -6.413639848186563 2315 46-47 -3.238331963906653 2315 48-49 -2.199643535904446 2315 50-51 -3.347891775626138 2315 52-53 -3.0271045481173324 2315 54-55 -1.6584919185272007 2315 56-57 -1.3657195412532133 2315 58-59 -2.4010596712734262 2315 60-61 -2.6848662557378553 2315 62-63 -4.623743619523079 2315 64-65 -4.108044240515881 2315 66-67 -3.4288630520617893 2315 68-69 -1.9760402230089618 2315 70-71 -3.33454021538099 2315 72-73 -2.4365309281135836 2315 74-75 -1.6001657509826401 2315 76-77 -3.149821926877628 2315 78-79 -1.6128166683684277 2315 80-81 -1.2739069658506956 2315 82-83 -0.8886487789844502 2315 84-85 -2.3962846622417295 2315 86-87 -1.983814308242998 2315 88-89 -1.3849958443123676 2315 90-91 -0.6349257758487887 2315 92-93 0.2386323285938623 2315 94-95 0.05130312964595518 2315 96-97 -0.274531843806578 2315 98-99 -0.4641597630568697 2315 100-101 -0.8610335029228882 2316 1 -7.33168662899859 2316 2 -5.986285412675048 2316 3 -3.3987281324686798 2316 4 -3.9342535027498684 2316 5 -2.0954496110691956 2316 6 -2.772057254534552 2316 7 -3.099499392873497 2316 8 -2.3779575561006823 2316 9 -7.052234661974719 2316 10-11 -6.7589835029280785 2316 12-13 -6.005645231665888 2316 14-15 -4.778130084372513 2316 16-17 -4.6763078980100445 2316 18-19 -3.9467239155398737 2316 20-21 -2.684016670425983 2316 22-23 -3.2584941699471273 2316 24-25 -3.028600838087172 2316 26-27 -3.900897497458004 2316 28-29 -4.109148137692124 2316 30-31 -6.598646223033434 2316 32-33 -4.548952104340529 2316 34-35 -5.219157775230652 2316 36-37 -3.9627004368244982 2316 38-39 -6.157553437411913 2316 40-41 -3.6292939415379806 2316 42-43 -4.326566253403566 2316 44-45 -2.8855039700596627 2316 46-47 -3.9239226935567117 2316 48-49 -5.130343844407058 2316 50-51 -4.403360750887639 2316 52-53 -5.934484838690338 2316 54-55 -4.688189059233768 2316 56-57 -3.876499573141011 2316 58-59 -2.658077775352158 2316 60-61 -2.8059497414637313 2316 62-63 -3.821736773904952 2316 64-65 -2.655216524981789 2316 66-67 -3.793136670056363 2316 68-69 -3.1086210558222263 2316 70-71 -2.4578590966811227 2316 72-73 -2.006816621387614 2316 74-75 -2.1055172599602656 2316 76-77 -2.68992998633831 2316 78-79 -4.766401466561323 2316 80-81 -5.868915418682324 2316 82-83 -3.37026010572054 2316 84-85 -2.0881158276891263 2316 86-87 -2.269757859337698 2316 88-89 -1.059701500996642 2316 90-91 -0.856147920654994 2316 92-93 -0.8456531666929124 2316 94-95 -1.6251797249299749 2316 96-97 -3.458918693411654 2316 98-99 -4.8027309828992735 2316 100-101 -4.163138905002162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29693.0 3 9179.0 4 8303.0 5 9234.0 6 10224.0 7 12259.0 8 14760.0 9 18229.0 10 21813.0 11 26968.0 12 32365.0 13 36937.0 14 41281.0 15 47170.0 16 52502.0 17 56580.0 18 52026.0 19 50656.0 20 54511.0 21 60064.0 22 67831.0 23 76587.0 24 86291.0 25 98390.0 26 112124.0 27 128911.0 28 147890.0 29 170083.0 30 196064.0 31 224931.0 32 257301.0 33 294596.0 34 340795.0 35 401130.0 36 450617.0 37 387887.0 38 125182.0 39 2292.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.949518136851275 20.66259598944428 13.644943318255432 22.742942555449016 2 14.931964570112404 20.881004703757373 41.28949527450227 22.89753545162795 3 18.178336045705674 26.153803748752818 30.711397167584693 24.956463037956816 4 11.904110647045194 17.173367237210297 39.36503900332107 31.557483112423434 5 12.245793138713674 38.73356236286623 35.73901468510498 13.28162981331511 6 30.066539650611578 38.47153439022669 18.24955494078116 13.212371018380573 7 26.976027932942305 33.86572105187838 22.411988077912902 16.746262937266412 8 25.524250718115038 35.704097385550234 20.91343519851112 17.858216697823607 9 26.513631574743552 16.68379805187149 21.05360871599238 35.74896165739258 10-11 23.682507845872667 27.291086332766735 29.078445415474107 19.94796040588649 12-13 25.128792977091365 24.83095086181459 28.916484776159702 21.123771384934344 14-15 21.98145277412135 26.380868918707566 26.91624798025103 24.721430326920053 16-17 20.970881931976397 29.625694784121965 28.063265343956466 21.340157939945172 18-19 21.04488718745539 28.011986366292792 30.405789036482663 20.53733740976916 20-21 21.63512209083882 27.469686933065624 30.424286064588873 20.470904911506683 22-23 21.282290884268555 27.414671069676764 30.34609794862423 20.95694009743045 24-25 21.03668580222467 27.624181922189994 30.50976222671771 20.829370048867624 26-27 20.633539189144344 27.9102037136355 30.23413704761113 21.222120049609025 28-29 20.819045405667328 28.095341684607476 30.315772171329296 20.7698407383959 30-31 21.007809428351177 27.851994528429024 29.997416916154666 21.14277912706513 32-33 20.49207544907929 28.10152577902214 30.01847194706833 21.387926824830235 34-35 21.295893744509065 28.052947469211524 29.862678486573703 20.788480299705707 36-37 21.510831861720174 27.398549076044176 29.83169676947563 21.258922292760023 38-39 20.89509643319882 27.91899021493654 30.00540337939524 21.1805099724694 40-41 21.158740978328964 27.69853384318786 30.25844563499293 20.884279543490244 42-43 20.95048464205217 27.69538925648186 29.8192171884875 21.534908912978466 44-45 20.93760054561266 27.930676467909464 30.046304784502738 21.085418201975134 46-47 21.383193067255295 28.00979458415293 29.32134415220016 21.285668196391615 48-49 20.86549877349804 28.381219649727218 29.213361376727633 21.539920200047106 50-51 21.127310142457915 28.269585212613823 29.281336334233927 21.32176831069433 52-53 21.250670074479686 28.07339043529799 29.320508193050927 21.3554312971714 54-55 20.799780059531916 28.34434007212306 29.395183936636972 21.460695931708056 56-57 20.982352054066578 28.47100215461363 28.92834018632722 21.618305604992578 58-59 20.748217925574263 27.943595414692236 29.25819271356527 22.049993946168232 60-61 20.47511446722031 28.347705447674198 29.42688627758354 21.75029380752195 62-63 20.70633554772839 28.14182828778219 29.733660290992315 21.41817587349711 64-65 20.421608913277833 27.996644287721416 29.49832077656014 22.083426022440612 66-67 20.462238845599064 28.252801895242523 29.099104637263405 22.185854621895 68-69 20.964470167792047 27.862176897214468 29.336486816203045 21.836866118790436 70-71 20.82172465121633 27.626368457013804 29.150378838415946 22.40152805335392 72-73 20.532756646373905 27.900633547608493 29.067075781038966 22.49953402497864 74-75 20.590688109194026 28.236246695722933 28.9053155934757 22.267749601607346 76-77 20.651893693122435 27.714356924764427 29.26264608451562 22.371103297597514 78-79 20.67114684787251 27.86046959817557 29.12569846691197 22.34268508703995 80-81 20.897350766055663 28.0021778013463 28.678921479101188 22.42154995349685 82-83 20.854208549462143 27.769374613841467 28.89383732328618 22.482579513410208 84-85 20.647798225191114 27.808614304693645 28.824758587523046 22.71882888259219 86-87 20.691904234879203 27.773617009937944 28.8902914958875 22.64418725929535 88-89 20.702146196810205 27.636019219843377 28.763267411440356 22.898567171906063 90-91 20.48838167775529 27.560921075120003 29.052742208187997 22.89795503893671 92-93 21.0026106681083 27.236761014468552 28.953146081270308 22.80748223615284 94-95 20.411657537944198 26.950570114029947 29.378443958939265 23.259328389086587 96-97 20.898465529448863 26.568866728941938 29.053780842620082 23.478886898989117 98-99 20.78006173749886 26.847051197034467 28.745634624611938 23.627252440854736 100-101 20.75808805846118 26.75089470463437 28.967392986658602 23.52362425024585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5481.0 1 3226.0 2 905.0 3 868.5 4 785.5 5 739.5 6 991.0 7 1640.0 8 2503.5 9 2892.0 10 2678.0 11 2494.0 12 2661.0 13 2938.5 14 3213.0 15 3587.0 16 4049.5 17 4550.5 18 5112.0 19 5801.5 20 6929.0 21 8526.0 22 10537.0 23 13242.0 24 16488.5 25 20867.5 26 27270.0 27 35266.0 28 42680.5 29 50501.0 30 60450.0 31 73997.5 32 91818.5 33 103829.5 34 110473.0 35 120827.0 36 136385.5 37 150329.0 38 155918.5 39 157949.5 40 159462.5 41 160794.0 42 159115.5 43 159647.0 44 162275.5 45 163903.5 46 160728.0 47 154959.0 48 153281.0 49 146690.0 50 137421.0 51 129401.5 52 120996.0 53 114778.5 54 108035.0 55 97770.5 56 87228.5 57 76909.5 58 68349.5 59 57999.0 60 44840.0 61 35385.0 62 28889.5 63 21080.0 64 14044.0 65 10165.0 66 7675.0 67 6177.5 68 4833.5 69 3478.5 70 2477.5 71 1733.5 72 1237.5 73 929.5 74 649.5 75 449.5 76 341.5 77 251.0 78 174.0 79 128.0 80 89.5 81 55.5 82 43.0 83 31.5 84 21.5 85 15.0 86 9.0 87 3.5 88 2.5 89 4.5 90 7.0 91 5.5 92 3.0 93 4.0 94 4.0 95 3.0 96 2.0 97 1.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13078903451064824 2 0.11612243619317762 3 0.12253017332216963 4 0.13482353566593952 5 0.12279122927927671 6 0.1373866305175363 7 0.16636384175642246 8 0.15333477626080536 9 0.16498736489167604 10-11 0.1582236423666289 12-13 0.14774580554274008 14-15 0.15307372030369826 16-17 0.14794753060050464 18-19 0.16319557173153196 20-21 0.16839295851393657 22-23 0.20178438866390613 24-25 0.2240572082770876 26-27 0.2369794781538882 28-29 0.23225673856622372 30-31 0.22258580197339317 32-33 0.22410467299656167 34-35 0.2135437729135933 36-37 0.2223603445558916 38-39 0.21074335446462644 40-41 0.21496771449781377 42-43 0.20762254915920997 44-45 0.20113174877113843 46-47 0.2156678191100555 48-49 0.2526547017601817 50-51 0.2538294535671636 52-53 0.25547885256888553 54-55 0.2539481153658486 56-57 0.22911220090107023 58-59 0.2303700159671316 60-61 0.20224716967877776 62-63 0.195044398498596 64-65 0.1967293960399235 66-67 0.20411015991813286 68-69 0.20338632294615414 70-71 0.20953300411803905 72-73 0.2398036289625921 74-75 0.24212940021681884 76-77 0.25686719561350047 78-79 0.2539599815457171 80-81 0.2670483779406767 82-83 0.26590922467330036 84-85 0.24248538561287394 86-87 0.2461520351922416 88-89 0.24918977723857857 90-91 0.4394046405306935 92-93 0.5904967087963516 94-95 0.34090348144224397 96-97 0.28196416603538593 98-99 0.3115940171670397 100-101 0.3309714888923064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4213656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.611472588683995 #Duplication Level Percentage of deduplicated Percentage of total 1 69.679015662877 28.29767434599412 2 13.201513941758513 10.72265843149711 3 5.860935255974268 7.140636344761518 4 3.216600928686116 5.225236017762868 5 2.0298544648573063 4.121768947928516 6 1.3276412359569407 3.23504793970031 7 0.9266214121703279 2.6342022056310053 8 0.6694257359172495 2.1749091939490395 9 0.4909872348875663 1.7945743167927135 >10 2.341506113128682 16.392776251013586 >50 0.1405606808803249 3.9742155210049472 >100 0.10301360494721953 8.178031474712071 >500 0.007672124816700388 2.098061478073412 >1k 0.004349550989742935 3.0809006078496832 >5k 2.4164172165238497E-4 0.6190799889987884 >10k+ 6.041043041309624E-5 0.310226934330256 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12645 0.3000956888744596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8916 0.2115977194151587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6158 0.14614387126049208 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5104 0.1211299640976862 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5056 0.11999081083030982 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3732359737007482E-5 0.0 5 0.0 0.0 0.0 2.3732359737007482E-5 0.0 6 0.0 0.0 0.0 2.3732359737007482E-5 0.0 7 0.0 0.0 0.0 2.3732359737007482E-5 0.0 8 0.0 0.0 0.0 2.3732359737007482E-5 2.3732359737007482E-5 9 0.0 0.0 0.0 1.1866179868503741E-4 2.3732359737007482E-5 10-11 0.0 0.0 0.0 1.5426033829054863E-4 2.3732359737007482E-5 12-13 0.0 0.0 0.0 2.1359123763306734E-4 9.492943894802993E-5 14-15 0.0 0.0 0.0 2.610559571070823E-4 1.0679561881653367E-4 16-17 0.0 0.0 0.0 3.559853960551122E-4 1.4239415842204488E-4 18-19 0.0 0.0 0.0 4.983795544771571E-4 1.6612651815905237E-4 20-21 0.0 0.0 0.0 6.289075330306983E-4 1.8985887789605985E-4 22-23 0.0 0.0 0.0 0.0011391532673763593 1.8985887789605985E-4 24-25 0.0 0.0 0.0 0.002183377095804688 1.8985887789605985E-4 26-27 0.0 0.0 0.0 0.00418876149358182 2.8478831684408977E-4 28-29 0.0 0.0 0.0 0.011047413457576982 3.3225303631810474E-4 30-31 2.3732359737007482E-5 0.0 0.0 0.0355392087061687 3.3225303631810474E-4 32-33 2.3732359737007482E-5 0.0 0.0 0.09287658983077879 3.9158393566062345E-4 34-35 2.3732359737007482E-5 0.0 0.0 0.17712646689715533 4.271824752661347E-4 36-37 2.3732359737007482E-5 0.0 0.0 0.31219919234033344 4.271824752661347E-4 38-39 2.3732359737007482E-5 0.0 0.0 0.5255293740162937 4.5091483500314216E-4 40-41 2.3732359737007482E-5 0.0 0.0 0.7603966721535882 4.5091483500314216E-4 42-43 2.3732359737007482E-5 0.0 0.0 0.9966641795153661 4.627810148716459E-4 44-45 2.3732359737007482E-5 0.0 0.0 1.2790911265656237 4.7464719474014965E-4 46-47 4.7464719474014964E-5 0.0 0.0 1.6111068392863586 4.7464719474014965E-4 48-49 7.119707921102244E-5 0.0 0.0 1.9529121503986087 5.221119142141646E-4 50-51 7.119707921102244E-5 0.0 0.0 2.3233742858932955 5.221119142141646E-4 52-53 7.119707921102244E-5 0.0 0.0 2.6809616162306558 5.45844273951172E-4 54-55 7.119707921102244E-5 0.0 0.0 3.0603708513461942 5.45844273951172E-4 56-57 7.119707921102244E-5 0.0 0.0 3.482723791405848 5.45844273951172E-4 58-59 7.119707921102244E-5 0.0 0.0 3.9359525314833483 5.695766336881795E-4 60-61 7.119707921102244E-5 0.0 0.0 4.42564604229676 5.695766336881795E-4 62-63 7.119707921102244E-5 0.0 0.0 4.896258735881619 5.93308993425187E-4 64-65 7.119707921102244E-5 0.0 0.0 5.364723650910278 6.170413531621945E-4 66-67 7.119707921102244E-5 0.0 0.0 5.900908854448488 6.170413531621945E-4 68-69 7.119707921102244E-5 0.0 0.0 6.472918055009711 6.170413531621945E-4 70-71 7.119707921102244E-5 0.0 0.0 7.064755642131203 6.170413531621945E-4 72-73 7.119707921102244E-5 0.0 0.0 7.653887740242678 6.40773712899202E-4 74-75 7.119707921102244E-5 0.0 0.0 8.237383402916612 6.763722525047133E-4 76-77 7.119707921102244E-5 0.0 0.0 8.85336866607051 6.88238432373217E-4 78-79 7.119707921102244E-5 0.0 0.0 9.531414049936682 7.119707921102244E-4 80-81 7.119707921102244E-5 0.0 0.0 10.21440763080802 7.119707921102244E-4 82-83 8.306325907952618E-5 0.0 0.0 10.864757350861105 7.475693317157356E-4 84-85 9.492943894802993E-5 0.0 0.0 11.48798098373479 7.594355115842394E-4 86-87 9.492943894802993E-5 0.0 0.0 12.119226154199584 7.713016914527432E-4 88-89 9.492943894802993E-5 0.0 0.0 12.773384917990457 7.831678713212469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15985 0.0 36.448616 1 GGTATCA 9505 0.0 35.34081 1 ATCAACG 19980 0.0 29.874887 3 TATCAAC 20075 0.0 29.755737 2 TCAACGC 20190 0.0 29.61151 4 CAACGCA 20140 0.0 29.472986 5 AACGCAG 20765 0.0 28.722992 6 TACATGG 15760 0.0 27.817383 2 GTACATG 15720 0.0 27.374744 1 ACATGGG 15965 0.0 26.272688 3 ACGCAGA 22970 0.0 25.59391 7 CGCAGAG 23195 0.0 25.244556 8 GCAGAGT 24455 0.0 22.701784 9 CATGGGG 6415 0.0 22.26364 4 GAGTACT 13085 0.0 21.900988 12-13 CATGGGA 8765 0.0 21.762083 4 AGTACTT 14465 0.0 20.680792 12-13 AGAGTAC 21125 0.0 20.629225 10-11 GTACTTT 13925 0.0 20.428225 14-15 TAAGGTG 2500 0.0 19.35916 5 >>END_MODULE