FastQCFastQC Report
Fri 27 May 2016
SRR939869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4213656
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133180.3160675669774657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104740.2485727358854164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74430.1766399535225467No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60800.1442927472010055No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA54260.1287717839330026No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52040.12350320007138693No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG44210.10492076239731007No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43500.10323576485598254No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43090.10226273810676524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94850.042.263041
GTATCAA156450.034.99741
ATCAACG193800.027.8565733
CAACGCA195250.027.6007485
TCAACGC195900.027.5332364
TATCAAC199450.027.0687392
AACGCAG202000.026.7300596
TACATGG157200.023.9957332
GTACATG159650.023.9569231
ACATGGG152550.023.52063
ACGCAGA228450.023.4908757
CGCAGAG229250.023.326978
AGGTAAG21000.022.6776812
TAAGGTG28250.022.0303365
CATGGGA83550.021.1616954
GCAGAGT249000.021.0036819
GAGTACT140400.020.8066212-13
GGTAAGG24750.020.3837723
GTACTTT148350.019.56467814-15
AGAGTAC222450.019.46502110-11
Produced by FastQC (version 0.11.5)