##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4213656 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.406815364139835 31.0 31.0 34.0 30.0 34.0 2 31.621218485799506 31.0 31.0 34.0 30.0 34.0 3 31.680875705088408 31.0 31.0 34.0 30.0 34.0 4 35.502147303909005 37.0 35.0 37.0 33.0 37.0 5 35.23382710881002 37.0 35.0 37.0 33.0 37.0 6 35.25688404558891 37.0 35.0 37.0 32.0 37.0 7 35.174655690924936 37.0 35.0 37.0 32.0 37.0 8 35.10411291287186 37.0 35.0 37.0 32.0 37.0 9 36.714270220445144 39.0 35.0 39.0 32.0 39.0 10-11 36.563812636817055 38.5 35.0 39.0 32.0 39.0 12-13 36.51564199830266 38.0 35.0 39.0 32.0 39.0 14-15 37.55209300426993 39.0 36.0 41.0 32.0 41.0 16-17 37.4853809850638 39.0 36.0 41.0 32.0 41.0 18-19 37.48043136411705 39.0 36.0 41.0 32.0 41.0 20-21 37.40637038239477 39.0 36.0 41.0 32.0 41.0 22-23 37.271357937145325 39.0 36.0 40.0 31.0 41.0 24-25 37.113885542626164 39.0 36.0 40.0 31.0 41.0 26-27 36.8255942108231 39.0 36.0 40.0 30.5 41.0 28-29 36.604483730992754 38.5 35.0 40.0 30.0 41.0 30-31 36.347590073798145 38.0 35.0 40.0 30.0 41.0 32-33 36.28659506139087 38.0 35.0 40.0 30.0 41.0 34-35 36.34630283060601 38.0 35.0 40.0 30.0 41.0 36-37 36.30322847902154 38.0 35.0 40.0 30.0 41.0 38-39 36.165462012086415 38.0 35.0 40.0 30.0 41.0 40-41 35.999791985866906 38.0 35.0 40.0 29.0 41.0 42-43 35.80110846732624 38.0 35.0 40.0 29.0 41.0 44-45 35.56660225704234 38.0 34.0 40.0 27.5 41.0 46-47 35.274396035177055 38.0 34.0 40.0 27.0 41.0 48-49 34.971759678530944 38.0 34.0 40.0 26.0 41.0 50-51 34.71318434632538 38.0 33.0 40.0 25.5 41.0 52-53 34.441824273267684 38.0 33.0 40.0 24.0 41.0 54-55 34.15713729359967 38.0 33.0 40.0 24.0 41.0 56-57 32.98199342803494 36.5 32.0 40.0 16.0 41.0 58-59 29.766109407127683 35.0 25.0 39.0 8.0 40.0 60-61 29.743352684699463 34.5 25.5 38.5 9.5 40.0 62-63 31.554670457199165 35.0 30.0 39.0 12.5 40.0 64-65 31.124443001516973 35.0 30.0 39.0 6.5 40.0 66-67 25.670135507027624 29.0 18.0 36.0 2.0 39.0 68-69 17.827866346944315 13.5 7.0 29.5 2.0 35.5 70-71 16.692060172923465 15.0 7.5 26.0 2.0 33.5 72-73 18.899818115194975 19.5 7.5 30.0 2.0 35.0 74-75 19.64445294062923 20.0 8.5 31.5 2.0 35.5 76-77 20.678560376072465 23.5 9.5 31.5 2.0 34.5 78-79 23.84864806239522 29.0 12.0 34.0 2.0 36.0 80-81 26.52941910777719 31.5 23.5 35.0 2.0 36.5 82-83 27.407807139453244 33.0 26.0 35.0 2.0 36.0 84-85 27.357262908979756 33.0 26.0 35.0 2.0 36.0 86-87 27.059501772332624 33.0 26.0 35.0 2.0 36.0 88-89 26.607662443255926 33.0 25.5 35.0 2.0 35.5 90-91 25.03573761123357 32.5 15.5 34.5 2.0 35.0 92-93 23.007191735632905 31.0 2.0 34.0 2.0 35.0 94-95 18.506147393142676 24.0 2.0 33.0 2.0 35.0 96-97 17.185404076649824 16.0 2.0 32.5 2.0 34.5 98-99 16.06106086970555 6.5 2.0 32.0 2.0 34.0 100-101 15.404699505607482 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.45461760648203864 1101 2 0.4327638019147493 1101 3 0.49316717913373864 1101 4 0.38840504362929096 1101 5 0.5056660124492538 1101 6 0.49767836155906053 1101 7 0.4654465483978143 1101 8 0.4668860425413328 1101 9 0.5883853714681564 1101 10-11 0.5664483946215313 1101 12-13 0.5457104356388811 1101 14-15 0.614808239738494 1101 16-17 0.6253442143516494 1101 18-19 0.6814372796988053 1101 20-21 0.7170732576359597 1101 22-23 0.7848538429648428 1101 24-25 0.7228535557888804 1101 26-27 0.9839443210453851 1101 28-29 0.8493628358242589 1101 30-31 0.8034629163646727 1101 32-33 0.7163073504757236 1101 34-35 0.9348933326483717 1101 36-37 0.8782951874982814 1101 38-39 0.8240487444253901 1101 40-41 0.9368961932211235 1101 42-43 1.019559864798147 1101 44-45 0.8164571097667093 1101 46-47 0.6399198777440276 1101 48-49 0.6006385463871027 1101 50-51 0.581282844052275 1101 52-53 0.7982979956187108 1101 54-55 0.8183320172149067 1101 56-57 1.315378180388052 1101 58-59 -7.8726219820469545 1101 60-61 -9.351747573757834 1101 62-63 -2.1215513917248323 1101 64-65 0.465017255967755 1101 66-67 -0.7860764476018005 1101 68-69 -3.4514063723252804 1101 70-71 -3.963815686772481 1101 72-73 -6.478303292456525 1101 74-75 -7.570588598799212 1101 76-77 -8.803008609860298 1101 78-79 -11.785156224451814 1101 80-81 -7.428894861790614 1101 82-83 -3.4493898488085826 1101 84-85 -2.4115461969083256 1101 86-87 -2.15021728334958 1101 88-89 -2.3707229535645666 1101 90-91 -8.225485511509522 1101 92-93 -14.386793176003591 1101 94-95 -12.642626411734923 1101 96-97 -12.5345443076244 1101 98-99 -12.145039267446249 1101 100-101 -11.962709184961057 1102 1 0.41968813312805864 1102 2 0.47215772677666124 1102 3 0.39931856676503585 1102 4 0.3253040570915715 1102 5 0.4133614617109629 1102 6 0.4036201939860504 1102 7 0.3569507075500269 1102 8 0.3767319140751795 1102 9 0.6030172095171125 1102 10-11 0.5217742696929548 1102 12-13 0.5079845322847589 1102 14-15 0.6360541151311025 1102 16-17 0.6604773878549963 1102 18-19 0.634412266569548 1102 20-21 0.7539683565757969 1102 22-23 0.7317103531892784 1102 24-25 0.7460515622597796 1102 26-27 0.771437444143956 1102 28-29 0.7127345154923077 1102 30-31 0.6383228762759501 1102 32-33 0.5749927556973873 1102 34-35 0.5304099107624438 1102 36-37 0.5618953793656445 1102 38-39 0.4782928060892644 1102 40-41 0.552442412411402 1102 42-43 0.678958493778488 1102 44-45 0.44917603779871484 1102 46-47 0.4440617101487163 1102 48-49 0.42638508540836995 1102 50-51 0.45951409552510114 1102 52-53 0.475103814450371 1102 54-55 0.3388183525761761 1102 56-57 0.9649380865778028 1102 58-59 -8.518078052350443 1102 60-61 -9.663872689924911 1102 62-63 -2.7815602289544152 1102 64-65 -0.12429078917884695 1102 66-67 -1.1513722656092966 1102 68-69 -3.584869059621518 1102 70-71 -4.167545947848824 1102 72-73 -6.623507651160164 1102 74-75 -7.672773359574492 1102 76-77 -8.813773713866706 1102 78-79 -12.024415237695502 1102 80-81 -7.908788881950514 1102 82-83 -3.7615613035533038 1102 84-85 -2.7139000628226633 1102 86-87 -2.598419508248149 1102 88-89 -2.635923540497579 1102 90-91 -8.31788034161869 1102 92-93 -14.283684822133885 1102 94-95 -12.487203142006816 1102 96-97 -12.37678749181071 1102 98-99 -11.881537036781843 1102 100-101 -11.644677377849094 1103 1 0.23400372641708245 1103 2 0.26135598063272525 1103 3 0.23412776523111134 1103 4 0.15761614149657532 1103 5 0.1968893459641663 1103 6 0.2332747610907191 1103 7 0.2744099067656265 1103 8 0.37034026326309544 1103 9 0.29934153869105984 1103 10-11 0.34309393190180515 1103 12-13 0.38612278909737086 1103 14-15 0.4360149378891123 1103 16-17 0.43590434708623604 1103 18-19 0.4628756557513114 1103 20-21 0.4166683602419994 1103 22-23 0.4669006342688604 1103 24-25 0.5273291973602099 1103 26-27 0.4005397345304118 1103 28-29 0.4265424889537428 1103 30-31 0.48280357036777133 1103 32-33 0.36408498172098547 1103 34-35 0.39307304470262494 1103 36-37 0.19315615163340993 1103 38-39 0.2539358072052451 1103 40-41 0.31402913225836215 1103 42-43 0.4460047627300838 1103 44-45 0.37010313595813926 1103 46-47 0.32645753043443193 1103 48-49 0.3117427512262694 1103 50-51 0.3814655398869391 1103 52-53 0.4257804921057229 1103 54-55 0.2588423754456244 1103 56-57 0.9658928142934471 1103 58-59 -6.679002616172376 1103 60-61 -8.628938189411446 1103 62-63 -2.3793496118095483 1103 64-65 -0.11048381507956151 1103 66-67 -1.6509099763996176 1103 68-69 -4.108167419584015 1103 70-71 -4.836048483954851 1103 72-73 -7.635231825410521 1103 74-75 -8.32653900948749 1103 76-77 -9.11940002170472 1103 78-79 -12.309852914243633 1103 80-81 -8.404516356117142 1103 82-83 -4.490865538080918 1103 84-85 -3.5822750286660963 1103 86-87 -3.3593572322098346 1103 88-89 -3.4173522870150386 1103 90-91 -9.134326850114025 1103 92-93 -15.053577869610717 1103 94-95 -12.83233903588938 1103 96-97 -12.894897618263148 1103 98-99 -12.475742723814967 1103 100-101 -12.06129778658709 1104 1 0.14858698601528886 1104 2 0.1437516860685797 1104 3 0.1506814672813377 1104 4 0.18707729428142272 1104 5 0.015470186186874457 1104 6 0.13415134349455826 1104 7 0.061852605577840336 1104 8 0.16442257670171045 1104 9 0.14154812155241814 1104 10-11 0.19347425170556676 1104 12-13 0.16625525527680196 1104 14-15 0.1995014168086442 1104 16-17 0.2549623435183719 1104 18-19 0.24738762894558874 1104 20-21 0.17290160438339797 1104 22-23 0.24667857235360913 1104 24-25 0.3787768901148709 1104 26-27 0.2571582621086961 1104 28-29 0.22515288269597278 1104 30-31 0.3548066509855161 1104 32-33 0.19677364222200566 1104 34-35 0.19954691234924127 1104 36-37 0.19170099184806588 1104 38-39 0.21187990298043502 1104 40-41 0.3725267474604479 1104 42-43 0.18782910747535908 1104 44-45 0.0447252835835954 1104 46-47 0.021748366244068507 1104 48-49 0.06955790763921499 1104 50-51 -0.03001947747043232 1104 52-53 -0.04777473337222915 1104 54-55 -0.22283239465183868 1104 56-57 0.5487775982497567 1104 58-59 -6.4050487941700815 1104 60-61 -8.724085497093682 1104 62-63 -2.6834750335625053 1104 64-65 -0.4816657141531202 1104 66-67 -2.0022662615724727 1104 68-69 -4.229729456221671 1104 70-71 -4.967092858259404 1104 72-73 -7.961415632725256 1104 74-75 -8.876977458950176 1104 76-77 -9.466680228913141 1104 78-79 -12.294440209970947 1104 80-81 -8.629178471779674 1104 82-83 -4.89949531540994 1104 84-85 -3.9586057259505587 1104 86-87 -3.787117618887887 1104 88-89 -3.844723735871252 1104 90-91 -9.439758378785458 1104 92-93 -15.224233714693954 1104 94-95 -12.901290134367764 1104 96-97 -12.628581724594815 1104 98-99 -11.874356153286442 1104 100-101 -11.433379733819882 1105 1 0.1143362485340802 1105 2 0.15342759711726828 1105 3 0.1332574430958644 1105 4 0.08478112789947545 1105 5 0.0902513789348518 1105 6 0.14677382287325003 1105 7 0.1474604047973216 1105 8 0.23627267020209786 1105 9 0.24267111856163126 1105 10-11 0.18157356554961268 1105 12-13 0.07882276103903507 1105 14-15 0.1781784541816478 1105 16-17 0.2530011995184367 1105 18-19 0.23247638207624988 1105 20-21 0.2671986498762209 1105 22-23 0.2755066667757191 1105 24-25 0.2748984263173355 1105 26-27 0.43054493167070973 1105 28-29 0.2826701416679711 1105 30-31 0.21820173806332122 1105 32-33 0.2182798259147276 1105 34-35 0.3091299651230415 1105 36-37 0.08869711494576649 1105 38-39 0.22969904547374398 1105 40-41 0.19162071746618636 1105 42-43 0.21128176872668547 1105 44-45 0.1671591306363993 1105 46-47 0.18984657974016983 1105 48-49 0.18140105172729903 1105 50-51 0.1477858771086673 1105 52-53 0.23940520620494965 1105 54-55 0.016866655809188558 1105 56-57 0.7153815859457069 1105 58-59 -6.31176358719522 1105 60-61 -8.680436197536654 1105 62-63 -2.6933283282873397 1105 64-65 -0.44435800871130127 1105 66-67 -1.6712145449275368 1105 68-69 -4.033997770082932 1105 70-71 -4.601840386781619 1105 72-73 -6.012753795914737 1105 74-75 -6.699603341526997 1105 76-77 -8.843734515760715 1105 78-79 -12.144981802977053 1105 80-81 -8.05112739228581 1105 82-83 -3.950891925462681 1105 84-85 -2.966608568175552 1105 86-87 -2.77537702314142 1105 88-89 -2.902604518279926 1105 90-91 -8.349265706022805 1105 92-93 -11.571449382014116 1105 94-95 -11.834476686539873 1105 96-97 -12.144001335130017 1105 98-99 -11.75435452757344 1105 100-101 -11.309264718565323 1106 1 0.10003579321091749 1106 2 0.134375230955893 1106 3 0.08951638765757153 1106 4 0.057567124013836235 1106 5 0.0566272723916299 1106 6 0.011171718952432741 1106 7 0.006963426959849528 1106 8 0.09525001197829397 1106 9 0.1342240543766522 1106 10-11 0.12028218071159102 1106 12-13 0.10602139926699294 1106 14-15 0.10753058308727503 1106 16-17 0.06224033212581048 1106 18-19 0.1621053176834053 1106 20-21 0.11816026521409384 1106 22-23 0.11232020914195573 1106 24-25 0.11719031285031889 1106 26-27 0.222007347521874 1106 28-29 0.14639612127236745 1106 30-31 0.1469435130641159 1106 32-33 0.15299439445706042 1106 34-35 0.2909885700912227 1106 36-37 0.21665976365535045 1106 38-39 0.11589853618338708 1106 40-41 0.1836468155985287 1106 42-43 0.11598980623987387 1106 44-45 0.13741586454218435 1106 46-47 0.03457765824443726 1106 48-49 -0.03445233441392048 1106 50-51 0.02282575286420041 1106 52-53 0.08486266806813347 1106 54-55 -0.12879428234042223 1106 56-57 0.7005704910913835 1106 58-59 -6.639152609669555 1106 60-61 -8.955297082118829 1106 62-63 -2.587272635148768 1106 64-65 -0.2975102421533684 1106 66-67 -1.7204332949275383 1106 68-69 -4.055624496033554 1106 70-71 -4.700006052057132 1106 72-73 -6.130578933231249 1106 74-75 -6.683796602024614 1106 76-77 -8.741687310461538 1106 78-79 -12.155287686999568 1106 80-81 -8.129001869603602 1106 82-83 -4.193287174151479 1106 84-85 -3.161794120359822 1106 86-87 -2.968904458460745 1106 88-89 -3.0653388508057517 1106 90-91 -8.153918177320254 1106 92-93 -11.454528045186906 1106 94-95 -11.918740379214725 1106 96-97 -12.265714308642123 1106 98-99 -11.810201447646037 1106 100-101 -11.586681724026004 1107 1 0.06665138471627685 1107 2 0.11548650315234354 1107 3 0.09264963633778223 1107 4 -0.024464500926171695 1107 5 -0.01350825592383842 1107 6 0.004522032902201545 1107 7 0.022192990229726206 1107 8 0.020088037894147703 1107 9 -0.06326934667917783 1107 10-11 0.06254816815359732 1107 12-13 0.07350447697455564 1107 14-15 -0.027299969024383586 1107 16-17 0.1056451373569729 1107 18-19 0.14412294486305655 1107 20-21 0.07714582752412014 1107 22-23 0.058074513453270526 1107 24-25 0.17531301321826476 1107 26-27 0.24903914743388356 1107 28-29 0.19349820993818412 1107 30-31 0.1605735822530434 1107 32-33 0.2106815395138497 1107 34-35 0.05558610948491349 1107 36-37 -0.02823880257404454 1107 38-39 0.08129957201766302 1107 40-41 -0.00994795923750047 1107 42-43 0.09417979584153358 1107 44-45 0.1268628030320258 1107 46-47 0.09410550878835267 1107 48-49 0.17700870681947833 1107 50-51 0.2210935300198571 1107 52-53 0.1417430672042741 1107 54-55 0.07641738379300023 1107 56-57 0.7720825491620928 1107 58-59 -6.472993570115879 1107 60-61 -8.979777165305517 1107 62-63 -2.6712723201991615 1107 64-65 -0.4145062727884685 1107 66-67 -1.8663993153642657 1107 68-69 -4.273582997673291 1107 70-71 -4.754040156600405 1107 72-73 -6.085056091885864 1107 74-75 -6.861402435091325 1107 76-77 -9.304830957478014 1107 78-79 -12.596237884967895 1107 80-81 -8.409836061132957 1107 82-83 -4.3622992572181865 1107 84-85 -3.445523848603301 1107 86-87 -3.1969894648597226 1107 88-89 -3.2927828347683175 1107 90-91 -8.461089529903843 1107 92-93 -11.71772580854477 1107 94-95 -12.071904497955726 1107 96-97 -12.694926509689955 1107 98-99 -11.90312895424498 1107 100-101 -11.503697376521604 1108 1 0.010511033839083694 1108 2 -0.045616124467642294 1108 3 -0.015853084035036602 1108 4 -0.018012523141607062 1108 5 -0.21306485212097215 1108 6 -0.06871198702834391 1108 7 -0.020459740888853162 1108 8 -0.057175074755299704 1108 9 -0.0740603473917929 1108 10-11 0.0807141504853206 1108 12-13 -0.019846728246747602 1108 14-15 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2311 62-63 1.4099375611148766 2311 64-65 1.3018598475071776 2311 66-67 -0.23114317632972003 2311 68-69 -3.2530712132279884 2311 70-71 -0.8906863402303351 2311 72-73 -1.0514884517930803 2311 74-75 1.350416281806499 2311 76-77 4.436418385532225 2311 78-79 5.257143155929544 2311 80-81 3.0150312245849555 2311 82-83 1.6719375956997844 2311 84-85 1.3695005787225796 2311 86-87 1.1571237141433102 2311 88-89 1.3766163451580233 2311 90-91 2.9322867675040207 2311 92-93 4.876588044983418 2311 94-95 8.915177790540582 2311 96-97 9.581715353556508 2311 98-99 10.124057634607773 2311 100-101 9.419599668411323 2312 1 -0.2996183627217448 2312 2 -0.23020530966358166 2312 3 -0.2629697673464726 2312 4 -0.18361022486134004 2312 5 -0.18919877492286474 2312 6 -0.25702349829886373 2312 7 -0.2657981582781517 2312 8 -0.2707682458175853 2312 9 -0.39254574612060367 2312 10-11 -0.4003705935297859 2312 12-13 -0.40111708158404724 2312 14-15 -0.5432232858355306 2312 16-17 -0.4253029627398419 2312 18-19 -0.34443436007173744 2312 20-21 -0.1989444362806836 2312 22-23 -0.25646665000042645 2312 24-25 -0.29650453183109704 2312 26-27 -0.38882551931903464 2312 28-29 -0.4482893988651213 2312 30-31 -0.3970704217979417 2312 32-33 -0.22502512988916834 2312 34-35 -0.22136476562605623 2312 36-37 -0.29409809775614093 2312 38-39 -0.3627415088994326 2312 40-41 -0.4306008460106767 2312 42-43 -0.32113453474206466 2312 44-45 -0.31713767880322763 2312 46-47 -0.2814123525769361 2312 48-49 -0.25194245382351 2312 50-51 -0.2039900103985346 2312 52-53 -0.25225362240905014 2312 54-55 -0.251637027899541 2312 56-57 0.874924484890613 2312 58-59 4.427735874925897 2312 60-61 4.1932628136364976 2312 62-63 1.4859563240145839 2312 64-65 1.4122181843661146 2312 66-67 -0.2307658135373103 2312 68-69 -0.9601973465487852 2312 70-71 0.1644351024975812 2312 72-73 7.303621225719418 2312 74-75 5.973782246116656 2312 76-77 6.0141261368769285 2312 78-79 5.922248081580246 2312 80-81 3.3763763122179853 2312 82-83 1.9694255791585888 2312 84-85 1.4985551545330864 2312 86-87 1.2835310064465446 2312 88-89 1.456081506299661 2312 90-91 2.9732965539801057 2312 92-93 5.028731140813452 2312 94-95 8.990096992164421 2312 96-97 9.655016606981096 2312 98-99 10.133452156920661 2312 100-101 9.391912568915014 2313 1 -0.33425536169010783 2313 2 -0.299173615335544 2313 3 -0.3435785771647524 2313 4 -0.3162974270236063 2313 5 -0.46273274728401503 2313 6 -0.32744062602728974 2313 7 -0.3965006798848947 2313 8 -0.4789897924576678 2313 9 -0.520699329835125 2313 10-11 -0.4991724907646784 2313 12-13 -0.5403868630153852 2313 14-15 -0.5960414044514835 2313 16-17 -0.5456668196748069 2313 18-19 -0.6457705319469298 2313 20-21 -0.631966434046582 2313 22-23 -0.5926868338463578 2313 24-25 -0.5878538083039828 2313 26-27 -0.6764478531101403 2313 28-29 -0.8465431897817624 2313 30-31 -0.8486641216842727 2313 32-33 -0.6646434468856555 2313 34-35 -0.6494126566557341 2313 36-37 -0.8287034160489171 2313 38-39 -1.0110474154025795 2313 40-41 -1.1299729822788933 2313 42-43 -0.8253834646878673 2313 44-45 -0.7966859867576019 2313 46-47 -0.7735547573887587 2313 48-49 -0.9292164874666327 2313 50-51 -0.6873631035631789 2313 52-53 -0.714114049079491 2313 54-55 -0.8486435847491975 2313 56-57 0.33133679803151495 2313 58-59 3.850471279848694 2313 60-61 3.6000481361020213 2313 62-63 1.0328272709447255 2313 64-65 0.8506023115074903 2313 66-67 1.4961477255232154 2313 68-69 0.24663801738166313 2313 70-71 2.6930146819042307 2313 72-73 8.149826467138414 2313 74-75 9.721171872218171 2313 76-77 8.562836225789358 2313 78-79 6.134701208182879 2313 80-81 3.30447747841691 2313 82-83 1.790704933229872 2313 84-85 1.283332616165339 2313 86-87 1.1858974435187442 2313 88-89 1.1207165913001376 2313 90-91 2.6755513254224113 2313 92-93 4.626941628972407 2313 94-95 8.70401797548358 2313 96-97 9.263687931698922 2313 98-99 9.593755854703119 2313 100-101 8.736915454306931 2314 1 -0.4074109489751656 2314 2 -0.32991889046965284 2314 3 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1.3034089005050653 2314 64-65 1.2639479337428803 2314 66-67 1.888561163917153 2314 68-69 -0.14379012424527104 2314 70-71 2.7272054035682842 2314 72-73 8.289658988826062 2314 74-75 10.027029621450389 2314 76-77 8.829145676510226 2314 78-79 6.490103594513037 2314 80-81 3.3262805063863645 2314 82-83 1.7089512504449793 2314 84-85 1.3372310764994992 2314 86-87 1.2668239965903396 2314 88-89 1.4916889040911698 2314 90-91 2.767057164255224 2314 92-93 4.8149428434383275 2314 94-95 8.809443234608501 2314 96-97 9.302904530530238 2314 98-99 9.666715521929564 2314 100-101 8.893573919148533 2315 1 -0.723592699051899 2315 2 -0.5513570425442467 2315 3 -0.5805280992079318 2315 4 -0.3166652508808738 2315 5 -0.6120724690522223 2315 6 -0.7318814371483953 2315 7 -0.6339625394083583 2315 8 -0.6978165496540001 2315 9 -0.9752031053275871 2315 10-11 -0.7977646382289123 2315 12-13 -0.6696411508453224 2315 14-15 -0.8052622668409697 2315 16-17 -0.9440047443880886 2315 18-19 -0.8706261641591695 2315 20-21 -0.9301571699713804 2315 22-23 -0.8756757711485008 2315 24-25 -0.929111905350787 2315 26-27 -0.8399931136284167 2315 28-29 -0.798559425985296 2315 30-31 -0.8852391706940352 2315 32-33 -0.8254250153875731 2315 34-35 -0.9512750002064081 2315 36-37 -1.041318867440019 2315 38-39 -0.915244317059674 2315 40-41 -0.9141377618981252 2315 42-43 -0.9225515354442066 2315 44-45 -0.887177241017362 2315 46-47 -0.9316652392612568 2315 48-49 -0.9705400007400087 2315 50-51 -0.9027372794572628 2315 52-53 -0.7719638185321784 2315 54-55 -0.7775347473515026 2315 56-57 0.584565066482142 2315 58-59 3.9110896404708377 2315 60-61 3.5377807889072876 2315 62-63 1.0545676615863222 2315 64-65 0.931023504687797 2315 66-67 1.8686413187981685 2315 68-69 6.265607014082505 2315 70-71 5.120157963401638 2315 72-73 8.858717286116395 2315 74-75 10.066447183658475 2315 76-77 8.787310316515654 2315 78-79 6.1696219840920605 2315 80-81 3.1832066453949928 2315 82-83 1.467930793940429 2315 84-85 0.9754042551822479 2315 86-87 0.9495574444812611 2315 88-89 1.0448045786314317 2315 90-91 2.278257535561533 2315 92-93 4.261365138563445 2315 94-95 8.307683166358231 2315 96-97 8.90717373518373 2315 98-99 9.237250112075365 2315 100-101 8.376789397046906 2316 1 -0.5126127515272643 2316 2 -0.534761620848883 2316 3 -0.6535417274924029 2316 4 -0.37377422702836327 2316 5 -0.551950987649036 2316 6 -0.5808203692529545 2316 7 -0.6603408435027873 2316 8 -0.7454147058661391 2316 9 -0.8206405872095175 2316 10-11 -0.6716288830911523 2316 12-13 -0.6851187411753088 2316 14-15 -0.8744935177007278 2316 16-17 -0.9841095979707859 2316 18-19 -0.950525751203223 2316 20-21 -0.8930560540538295 2316 22-23 -0.8647065924594628 2316 24-25 -2.326712203672301 2316 26-27 -1.6209145354216972 2316 28-29 -1.3221533036132413 2316 30-31 -1.0031637251158543 2316 32-33 -0.9107388446125881 2316 34-35 -0.9996304722321128 2316 36-37 -1.0315823245500368 2316 38-39 -1.2816437236030822 2316 40-41 -1.0527498226831185 2316 42-43 -1.1071180356513324 2316 44-45 -1.1970234783658 2316 46-47 -1.1934349402558126 2316 48-49 -1.3487654540123017 2316 50-51 -1.3189335887728078 2316 52-53 -1.2924806847534214 2316 54-55 -1.132869333179265 2316 56-57 0.07509864309951553 2316 58-59 3.4111973045399075 2316 60-61 3.3107965884454877 2316 62-63 0.8467715152070987 2316 64-65 0.4520107606427679 2316 66-67 5.697780116994174 2316 68-69 8.371161615430605 2316 70-71 5.131432210525482 2316 72-73 8.863976941832298 2316 74-75 9.663447773928613 2316 76-77 8.29228336188213 2316 78-79 5.789336899934849 2316 80-81 2.5761403997151575 2316 82-83 1.0951577133675876 2316 84-85 0.7239880828684448 2316 86-87 0.6338742019358001 2316 88-89 0.7700199602008588 2316 90-91 2.2005572293904727 2316 92-93 4.120343666175575 2316 94-95 8.064415680139621 2316 96-97 8.859840549644606 2316 98-99 9.146007642974197 2316 100-101 8.20948669552153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 36.0 4 76.0 5 186.0 6 370.0 7 1037.0 8 2377.0 9 4391.0 10 7206.0 11 11244.0 12 15618.0 13 20631.0 14 27087.0 15 37053.0 16 48613.0 17 62192.0 18 66439.0 19 67405.0 20 70023.0 21 68936.0 22 66139.0 23 64176.0 24 68125.0 25 76375.0 26 100446.0 27 143333.0 28 198537.0 29 259608.0 30 313605.0 31 342673.0 32 357805.0 33 374444.0 34 382678.0 35 372958.0 36 315565.0 37 198339.0 38 64977.0 39 2946.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86936541100947 20.876291963442615 14.778670776072964 23.475671849474956 2 14.953145484906969 21.820338970178486 40.25420390044495 22.972311644469592 3 17.88962019115754 26.35011657799903 31.336816433320802 24.423446797522622 4 12.218676607677512 17.980679960585295 38.93381424587105 30.866829185866145 5 11.97848674325229 39.135233004472546 35.87390421627819 13.01237603599697 6 30.086967252895647 39.031126183800055 17.79455128623487 13.087355277069431 7 26.731750290009437 33.93952899809571 22.403252662296115 16.92546804959873 8 25.346130780109327 36.28659028163923 20.682552644937775 17.684726293313663 9 25.899717489989694 16.5764599673063 21.00204667870372 36.52177586400029 10-11 23.54569286149605 27.47831099643635 29.082037071844496 19.8939590702231 12-13 24.80704404915826 24.90879654153068 29.028366340299254 21.2557930690118 14-15 21.521206621767337 26.632252310556993 26.92496775656536 24.92157331111031 16-17 20.741417904071906 29.81117822622445 28.106257368897698 21.34114650080595 18-19 20.75734481361258 28.238128774605574 30.39020648008444 20.614319931697402 20-21 21.27595370860839 27.623386911508675 30.425098774081228 20.675560605801707 22-23 21.0105309973097 27.588963123710148 30.377669653146818 21.022836225833338 24-25 20.88949596265096 27.82724788164957 30.412817277917327 20.870438877782142 26-27 20.517525615876888 28.118151344684467 30.134504638678163 21.22981840076048 28-29 20.715814020878444 28.211386966500346 30.242438705039575 20.830360307581643 30-31 20.828394094915737 27.95920854620665 29.97987674461575 21.232520614261865 32-33 20.33234218688407 28.278917581691264 29.9460039172791 21.442736314145563 34-35 21.104415602415415 28.215537103154446 29.801922534723246 20.878124759706893 36-37 21.322535878625132 27.516382055844744 29.848516428108862 21.31256563742126 38-39 20.758389715005055 28.031610530687402 29.996081976772686 21.213917777534856 40-41 20.995827996518443 27.808280362812017 30.24531010143776 20.950581539231777 42-43 20.79584443232698 27.81961125411249 29.780195415087924 21.604348898472605 44-45 20.79390700722419 27.976421297320048 30.04676262280426 21.182909072651505 46-47 21.23770170345829 28.087607172395977 29.331312619113415 21.34337850503232 48-49 20.75544932372562 28.42054510507776 29.19566501649127 21.62834055470535 50-51 21.006177334422926 28.28998398249053 29.278618612951334 21.42522007013521 52-53 21.172455301184616 28.04166948056287 29.36707096964707 21.418804248605444 54-55 20.711934203753646 28.295428934459277 29.438394111673176 21.554242750113907 56-57 20.920745899968686 28.29291685013486 29.02089487317074 21.765442376725716 58-59 19.367287816566247 27.786023929957622 30.763702639646922 22.082985613829205 60-61 20.64473744824499 28.24151573698794 29.368445699181166 21.745301115585907 62-63 20.532235786635784 28.246646604835725 29.62052999307013 21.60058761545837 64-65 19.79700148453478 28.040215646709004 29.84769288703098 22.31508998172524 66-67 20.568104594519273 27.898773827774896 29.458097004301926 22.075024573403905 68-69 21.773646964833038 26.99579420534688 29.630641405951362 21.59991742386872 70-71 20.756439136786618 27.149993410195773 29.850526735309046 22.243040717708563 72-73 20.270027109368367 27.432617777254297 29.838661891831954 22.45869322154538 74-75 20.722340265467096 27.65791405208649 29.217752653802897 22.401993028643517 76-77 18.92255348341121 27.82306347103387 30.389323876645236 22.86505916890968 78-79 19.62045960571073 28.034682735344113 29.341619365834166 23.003238293110993 80-81 20.2234595505534 28.150259804537566 28.513863935257056 23.112416709651978 82-83 20.31857901768503 27.944559210385012 28.577205716138877 23.159656055791082 84-85 20.031217568224054 27.89642383936219 28.54504155895134 23.527317033462413 86-87 20.11245669607904 27.97356755008208 28.42257317078091 23.491402583057972 88-89 20.026919631621347 27.86972160857555 28.35017165032673 23.75318710947637 90-91 19.742691916903947 27.828083386248874 28.487082562395837 23.94214213445134 92-93 19.677482779435795 27.610996055074423 28.739732625673327 23.971788539816455 94-95 18.791837614118435 27.331000236018998 29.425461460429787 24.45170068943278 96-97 18.59584390219979 26.967564127806774 29.79600555146464 24.640586418528795 98-99 18.64180764685153 27.170331711971308 29.258172935160704 24.92968770601646 100-101 18.22645125707162 27.130177856728306 29.548930706614502 25.094440179585575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 578.0 1 441.5 2 305.0 3 308.0 4 359.0 5 449.0 6 602.0 7 884.0 8 1255.0 9 1595.5 10 1827.0 11 2045.5 12 2349.0 13 2729.0 14 3164.0 15 3614.5 16 4011.0 17 4527.0 18 5125.0 19 5869.5 20 6982.0 21 8572.5 22 10547.5 23 13177.0 24 16756.0 25 21421.0 26 27646.5 27 35038.0 28 42859.5 29 51475.5 30 61630.0 31 75014.0 32 91080.0 33 105079.0 34 116117.0 35 127538.0 36 140660.5 37 151200.0 38 157366.0 39 161043.5 40 163412.5 41 165620.5 42 166991.5 43 168299.0 44 168869.5 45 167457.0 46 163791.5 47 159719.5 48 156138.0 49 150080.5 50 141564.0 51 132600.5 52 123515.0 53 115011.0 54 106238.5 55 95797.5 56 84106.0 57 71628.5 58 59818.0 59 48343.0 60 37453.5 61 28808.0 62 21981.5 63 15745.5 64 10790.0 65 7633.5 66 5605.5 67 4242.0 68 3053.0 69 2060.5 70 1369.0 71 939.0 72 669.0 73 483.0 74 312.5 75 198.5 76 134.0 77 91.5 78 53.5 79 31.0 80 22.5 81 13.0 82 5.5 83 2.5 84 2.0 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0209319412880406 2 0.004461683630557407 3 0.003987036435817257 4 0.0 5 0.06780335176863038 6 2.3732359737007482E-5 7 0.0 8 7.119707921102244E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.779926980275561E-4 16-17 0.0 18-19 0.0012696812459299003 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.2545741750157108E-4 28-29 0.0017917931601440648 30-31 0.00881657164229828 32-33 4.034501155291272E-4 34-35 0.001234082706324389 36-37 0.026580242905448383 38-39 0.025452955817940524 40-41 0.027802459431904264 42-43 0.02817031100782788 44-45 0.028728021461647556 46-47 0.03047234990231761 48-49 0.030270624844553045 50-51 0.025595349976362567 52-53 0.02213042545475948 54-55 0.016861841593143814 56-57 0.15481804874436833 58-59 0.7652380735399378 60-61 0.3136824648238964 62-63 3.797177557921197E-4 64-65 0.14098208301769297 66-67 1.4306697082058906 68-69 2.7439235666129367 70-71 2.2226541511694355 72-73 1.8730290275238415 74-75 1.5758286865372968 76-77 1.1290551483082625 78-79 0.42911666258470077 80-81 1.779926980275561E-4 82-83 1.779926980275561E-4 84-85 7.119707921102244E-5 86-87 0.0029190802476519204 88-89 5.9330899342518706E-5 90-91 0.22853075808751355 92-93 0.17782657150939707 94-95 1.3074750288110848 96-97 1.2823305936697253 98-99 1.0030719166443582 100-101 0.4085881714121893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4213656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.63362396569957 #Duplication Level Percentage of deduplicated Percentage of total 1 64.79456746061136 22.440706844509677 2 14.66563683810738 10.158483029370444 3 6.6812951234039115 6.941923887235002 4 3.8003983053225507 5.264862633056926 5 2.4102439987092152 4.173774215843952 6 1.6029543117756964 3.3309670120941735 7 1.1527079428122473 2.794571740354415 8 0.8124976684831765 2.2511790978603177 9 0.6288170067468419 1.9600390579416205 >10 3.102328093756924 18.702027499727784 >50 0.1863296181567374 4.46416693006519 >100 0.14421516177315685 9.825713763173763 >500 0.011478681525137936 2.6790473066853147 >1k 0.0061173811011111965 3.871192376137916 >5k 2.749384764544357E-4 0.57496915772978 >10k+ 1.3746923822721785E-4 0.5663754482137592 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13318 0.3160675669774657 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10474 0.2485727358854164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7443 0.1766399535225467 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6080 0.1442927472010055 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5426 0.1287717839330026 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5204 0.12350320007138693 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4421 0.10492076239731007 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4350 0.10323576485598254 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4309 0.10226273810676524 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3732359737007482E-5 2 0.0 0.0 0.0 0.0 2.3732359737007482E-5 3 0.0 0.0 0.0 0.0 2.3732359737007482E-5 4 0.0 0.0 0.0 2.3732359737007482E-5 2.3732359737007482E-5 5 0.0 0.0 0.0 2.3732359737007482E-5 2.3732359737007482E-5 6 0.0 0.0 0.0 2.3732359737007482E-5 7.119707921102244E-5 7 0.0 0.0 0.0 2.3732359737007482E-5 7.119707921102244E-5 8 0.0 0.0 0.0 2.3732359737007482E-5 7.119707921102244E-5 9 0.0 0.0 0.0 2.3732359737007482E-5 7.119707921102244E-5 10-11 0.0 0.0 0.0 7.119707921102244E-5 7.119707921102244E-5 12-13 0.0 0.0 0.0 1.4239415842204488E-4 1.5426033829054863E-4 14-15 0.0 0.0 0.0 1.6612651815905237E-4 2.017250577645636E-4 16-17 0.0 0.0 0.0 2.610559571070823E-4 2.610559571070823E-4 18-19 0.0 0.0 0.0 3.20386856449601E-4 2.8478831684408977E-4 20-21 0.0 0.0 0.0 4.865133746086534E-4 3.0852067658109725E-4 22-23 0.0 0.0 0.0 9.848929290858105E-4 3.0852067658109725E-4 24-25 0.0 0.0 0.0 0.002052849117251147 3.0852067658109725E-4 26-27 0.0 0.0 0.0 0.004259958572792843 3.441192161866085E-4 28-29 0.0 0.0 0.0 0.011379666493895088 3.559853960551122E-4 30-31 0.0 0.0 0.0 0.03692755175078364 3.559853960551122E-4 32-33 0.0 0.0 0.0 0.09678056300751652 4.034501155291272E-4 34-35 0.0 0.0 0.0 0.18434110425720562 4.5091483500314216E-4 36-37 0.0 0.0 0.0 0.32526385637555605 4.5091483500314216E-4 38-39 0.0 0.0 2.3732359737007482E-5 0.5476716656509216 4.865133746086534E-4 40-41 0.0 0.0 2.3732359737007482E-5 0.7926726813959184 4.983795544771571E-4 42-43 0.0 0.0 2.3732359737007482E-5 1.038634857710264 4.983795544771571E-4 44-45 0.0 0.0 2.3732359737007482E-5 1.3318006975415173 5.102457343456608E-4 46-47 0.0 0.0 2.3732359737007482E-5 1.6661421815164787 5.339780940826684E-4 48-49 0.0 0.0 2.3732359737007482E-5 1.9858170671739697 5.577104538196757E-4 50-51 0.0 0.0 2.3732359737007482E-5 2.313679616940728 5.93308993425187E-4 52-53 0.0 0.0 2.3732359737007482E-5 2.6235055733073605 6.170413531621945E-4 54-55 0.0 0.0 2.3732359737007482E-5 2.9554144904092787 6.170413531621945E-4 56-57 0.0 0.0 2.3732359737007482E-5 3.2394315055619156 6.170413531621945E-4 58-59 0.0 0.0 2.3732359737007482E-5 3.434380974621564 6.170413531621945E-4 60-61 0.0 0.0 2.3732359737007482E-5 3.5821623787039094 6.170413531621945E-4 62-63 1.4239415842204488E-4 0.0 2.3732359737007482E-5 3.7071963159783334 6.40773712899202E-4 64-65 1.6612651815905237E-4 0.0 2.3732359737007482E-5 3.836229155868443 6.645060726362095E-4 66-67 1.6612651815905237E-4 0.0 2.3732359737007482E-5 3.9463591712280266 6.645060726362095E-4 68-69 1.6612651815905237E-4 0.0 2.3732359737007482E-5 4.068402831175588 6.645060726362095E-4 70-71 1.6612651815905237E-4 0.0 3.559853960551122E-5 4.284794487257622 6.645060726362095E-4 72-73 1.6612651815905237E-4 0.0 4.7464719474014964E-5 4.560778098639281 7.119707921102244E-4 74-75 1.6612651815905237E-4 0.0 4.7464719474014964E-5 4.881070025649935 7.713016914527432E-4 76-77 1.6612651815905237E-4 0.0 4.7464719474014964E-5 5.343471322765788 7.831678713212469E-4 78-79 1.6612651815905237E-4 0.0 4.7464719474014964E-5 6.001522193553532 8.069002310582544E-4 80-81 1.6612651815905237E-4 0.0 4.7464719474014964E-5 6.711250277668609 8.187664109267582E-4 82-83 1.6612651815905237E-4 0.0 4.7464719474014964E-5 7.381428384281963 9.018296700062843E-4 84-85 1.6612651815905237E-4 0.0 4.7464719474014964E-5 7.93152075062606 9.255620297432918E-4 86-87 1.6612651815905237E-4 0.0 4.7464719474014964E-5 8.420929473122627 9.374282096117955E-4 88-89 1.6612651815905237E-4 0.0 4.7464719474014964E-5 8.90848707155971 9.492943894802993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9485 0.0 42.26304 1 GTATCAA 15645 0.0 34.9974 1 ATCAACG 19380 0.0 27.856573 3 CAACGCA 19525 0.0 27.600748 5 TCAACGC 19590 0.0 27.533236 4 TATCAAC 19945 0.0 27.068739 2 AACGCAG 20200 0.0 26.730059 6 TACATGG 15720 0.0 23.995733 2 GTACATG 15965 0.0 23.956923 1 ACATGGG 15255 0.0 23.5206 3 ACGCAGA 22845 0.0 23.490875 7 CGCAGAG 22925 0.0 23.32697 8 AGGTAAG 2100 0.0 22.677681 2 TAAGGTG 2825 0.0 22.030336 5 CATGGGA 8355 0.0 21.161695 4 GCAGAGT 24900 0.0 21.003681 9 GAGTACT 14040 0.0 20.80662 12-13 GGTAAGG 2475 0.0 20.383772 3 GTACTTT 14835 0.0 19.564678 14-15 AGAGTAC 22245 0.0 19.465021 10-11 >>END_MODULE