FastQCFastQC Report
Fri 27 May 2016
SRR939868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544829
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137220.30192555099432783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97320.2141334690480104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67370.14823440001813049No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA56210.12367902070682968No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA55480.12207279965868903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101950.038.575351
GTATCAA171650.037.409311
ATCAACG211500.029.9833373
CAACGCA212850.029.8390145
TCAACGC214600.029.5727834
TATCAAC215700.029.5297222
AACGCAG219700.029.0381076
GTACATG171800.026.1499631
ACGCAGA246850.025.57757
TACATGG172500.025.362072
CGCAGAG248000.025.3442338
TAAGGTG26400.024.5923185
ACATGGG171650.024.3371433
AGGTAAG23350.023.7731082
GCAGAGT261650.022.8892759
GGTAAGG24850.022.5279143
GTAAGGT27750.021.6998624
CATGGGA99250.020.9982554
GAGTACT144250.020.92151812-13
CATGGGG70150.020.4541854
Produced by FastQC (version 0.11.5)