FastQCFastQC Report
Fri 27 May 2016
SRR939868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544829
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139530.30700825047543046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112650.24786411105896392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78520.17276777630137458No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA65490.1440978307434669No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58990.12979586250659814No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG55440.12198478754646214No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA47910.10541650741975112No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47910.10541650741975112No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG46940.10328221369824916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101350.039.1032031
GTATCAA162700.034.8310361
ATCAACG205400.027.3694933
CAACGCA208750.026.9075475
TCAACGC209350.026.8530884
TATCAAC211400.026.5988352
GTACATG172850.026.1956161
AACGCAG216150.025.9863556
TACATGG173900.025.6494182
ACATGGG171050.025.1000393
TAAGGTG28500.022.6382525
ACGCAGA247950.022.6344287
CGCAGAG250250.022.3695268
CATGGGA95800.022.135584
GTATAGG15600.021.2972261
GGTAAGG24500.020.525263
ATGGGAT48850.020.2969425
CATGGGG66000.020.1262574
GCAGAGT273500.020.0342719
AGGTAAG24000.019.7714252
Produced by FastQC (version 0.11.5)