##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4544829 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38530162520966 31.0 31.0 34.0 30.0 34.0 2 31.615325241059676 31.0 31.0 34.0 30.0 34.0 3 31.659263967907265 31.0 31.0 34.0 30.0 34.0 4 35.482719371839956 37.0 35.0 37.0 33.0 37.0 5 35.21887446150339 37.0 35.0 37.0 32.0 37.0 6 35.240070858551555 37.0 35.0 37.0 32.0 37.0 7 35.1443816698054 37.0 35.0 37.0 32.0 37.0 8 35.10048408862028 37.0 35.0 37.0 32.0 37.0 9 36.682794886232244 39.0 35.0 39.0 32.0 39.0 10-11 36.54618985664807 38.0 35.0 39.0 32.0 39.0 12-13 36.473144424135654 38.0 35.0 39.0 32.0 39.0 14-15 37.49994169197565 39.0 36.0 41.0 32.0 41.0 16-17 37.4241802056799 39.0 36.0 41.0 32.0 41.0 18-19 37.42229685649339 39.0 36.0 41.0 32.0 41.0 20-21 37.33901088467795 39.0 36.0 41.0 31.5 41.0 22-23 37.21722225412661 39.0 36.0 40.0 31.0 41.0 24-25 37.07070233005466 39.0 36.0 40.0 31.0 41.0 26-27 36.778002428694236 39.0 35.5 40.0 30.5 41.0 28-29 36.558787250301386 38.0 35.0 40.0 30.0 41.0 30-31 36.2856371493845 38.0 35.0 40.0 30.0 41.0 32-33 36.25165182232379 38.0 35.0 40.0 30.0 41.0 34-35 36.096202519390715 38.0 35.0 40.0 30.0 41.0 36-37 36.12427970337278 38.0 35.0 40.0 30.0 41.0 38-39 36.02292759529566 38.0 35.0 40.0 29.0 41.0 40-41 35.94343329529009 38.0 35.0 40.0 29.0 41.0 42-43 35.72353668311833 38.0 34.5 40.0 28.5 41.0 44-45 35.48505950388893 38.0 34.0 40.0 27.0 41.0 46-47 35.21744899533074 38.0 34.0 40.0 26.5 41.0 48-49 34.920043416374966 38.0 33.5 40.0 26.0 41.0 50-51 34.70426599108569 38.0 33.0 40.0 25.5 41.0 52-53 34.46228075907807 38.0 33.0 40.0 24.0 41.0 54-55 34.20821036391028 38.0 33.0 40.0 24.0 41.0 56-57 33.86441954141729 37.0 33.0 40.0 23.5 41.0 58-59 33.521111465359866 37.0 32.0 40.0 23.0 41.0 60-61 33.23295243891464 36.5 32.0 39.0 22.0 41.0 62-63 32.95790314663104 36.0 31.0 39.0 22.0 40.5 64-65 32.538608383285705 36.0 31.0 39.0 20.5 40.0 66-67 31.650600275609932 35.0 30.0 38.5 15.0 40.0 68-69 29.761046345198025 34.0 27.0 38.0 7.5 40.0 70-71 29.116093806829696 34.0 25.5 37.0 7.5 39.0 72-73 29.690534121305774 34.0 27.5 36.5 8.5 39.0 74-75 29.799767163957107 34.0 28.0 36.0 8.0 39.0 76-77 29.3254984951029 33.5 28.5 35.5 8.0 38.0 78-79 29.85887565846812 34.0 29.0 36.0 8.0 38.0 80-81 29.672937749693112 34.0 29.0 35.5 7.0 37.0 82-83 29.218922318089415 34.0 29.0 35.0 4.5 37.0 84-85 28.847515605097573 34.0 29.0 35.0 2.0 36.0 86-87 28.466760135529853 33.0 27.5 35.0 2.0 36.0 88-89 28.000146430151716 33.0 26.5 35.0 2.0 35.5 90-91 27.514854244241093 33.0 26.0 35.0 2.0 35.0 92-93 27.0425074298725 32.5 25.5 34.5 2.0 35.0 94-95 26.243305523706173 32.0 24.5 34.0 2.0 35.0 96-97 25.73777561267982 32.0 23.5 34.0 2.0 35.0 98-99 25.069264982246857 32.0 20.5 34.0 2.0 35.0 100-101 23.55579428840997 30.5 8.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28283801042785583 1101 2 0.2860304503161686 1101 3 0.3064720611954961 1101 4 0.24543020308090746 1101 5 0.28412309430789406 1101 6 0.215623406138576 1101 7 0.15641097785591285 1101 8 0.2336619671173068 1101 9 0.32785704100377444 1101 10-11 0.3412760167587692 1101 12-13 0.39757326509751323 1101 14-15 0.3917421580824083 1101 16-17 0.43512820601472413 1101 18-19 0.2720431799408587 1101 20-21 0.35355633740926606 1101 22-23 0.45659079846536343 1101 24-25 0.3693834677839547 1101 26-27 0.3058798958308202 1101 28-29 0.3686800176502416 1101 30-31 0.23723421208927675 1101 32-33 0.11225353420191198 1101 34-35 0.06246491679217314 1101 36-37 0.2593560427910262 1101 38-39 0.31686853314771923 1101 40-41 0.3643839767609691 1101 42-43 0.21886366865769702 1101 44-45 0.09283184626865193 1101 46-47 0.09284789493447221 1101 48-49 0.16479122197866047 1101 50-51 0.08531062551600854 1101 52-53 -0.10205076228829313 1101 54-55 -0.31186373230791276 1101 56-57 -0.17024539127370275 1101 58-59 -0.04979259464333552 1101 60-61 -0.29132513334052845 1101 62-63 -0.25525638502445247 1101 64-65 -0.36922107773771984 1101 66-67 -0.4283108780083893 1101 68-69 -9.017411091443861 1101 70-71 -6.762355027884684 1101 72-73 -1.7021101367135323 1101 74-75 -0.5047184888523866 1101 76-77 -0.3496472679707132 1101 78-79 -0.48186531819047573 1101 80-81 -0.5456520324738605 1101 82-83 -0.4037176215013396 1101 84-85 -0.28204729643317705 1101 86-87 -0.3552578163986979 1101 88-89 -0.28859353220908446 1101 90-91 -0.5374400285964462 1101 92-93 -0.774899376588781 1101 94-95 -0.35525635294280633 1101 96-97 -0.8471143720638743 1101 98-99 -0.6205375113033433 1101 100-101 -0.7246623776452168 1102 1 0.20658792282536353 1102 2 0.09690951215184995 1102 3 0.20474687606222375 1102 4 0.15543470000898196 1102 5 0.3035907047454458 1102 6 0.23785166319933637 1102 7 0.3129665645590194 1102 8 0.2887321075149032 1102 9 0.2713423354649578 1102 10-11 0.36235974722340814 1102 12-13 0.266209381854118 1102 14-15 0.44060843579399744 1102 16-17 0.4426833371848602 1102 18-19 0.38419306196949066 1102 20-21 0.33981194396894665 1102 22-23 0.32047944401399064 1102 24-25 0.2346876236463089 1102 26-27 0.37771452568107833 1102 28-29 0.24451384739770532 1102 30-31 0.16401867491077837 1102 32-33 0.006927886527464011 1102 34-35 -0.12089265323142229 1102 36-37 0.01845516793411406 1102 38-39 0.31543316626846973 1102 40-41 0.2011802367184501 1102 42-43 0.1620566627941713 1102 44-45 0.12172738321354615 1102 46-47 0.12488266728479402 1102 48-49 0.1482531849753599 1102 50-51 0.13711582092797414 1102 52-53 0.14984057557700936 1102 54-55 -0.05741323355771044 1102 56-57 -0.048187083053079505 1102 58-59 -0.1931477993995543 1102 60-61 0.06398635193056634 1102 62-63 0.032049575449306644 1102 64-65 -0.012025554809227401 1102 66-67 0.24006126323019217 1102 68-69 -4.433803562301431 1102 70-71 0.3100390898997887 1102 72-73 0.683424355308798 1102 74-75 0.3768425000045781 1102 76-77 0.04437809587164665 1102 78-79 -0.06822706977316173 1102 80-81 -0.016012896351266903 1102 82-83 0.014929445567130273 1102 84-85 -0.0015213310536807967 1102 86-87 -0.19045080469500775 1102 88-89 -0.34728238566762926 1102 90-91 -0.3722347175491869 1102 92-93 -0.37569860335075944 1102 94-95 -0.04223876820199024 1102 96-97 -0.648012297636793 1102 98-99 -0.32728669952022216 1102 100-101 -0.23718792837290437 1103 1 0.18393547234187224 1103 2 0.1566091290799072 1103 3 0.18497083457351948 1103 4 0.2186256721916564 1103 5 0.1657537134357483 1103 6 0.12744503366293003 1103 7 0.13124306353205384 1103 8 0.20161736247224837 1103 9 0.2814372682090678 1103 10-11 0.3026652793669058 1103 12-13 0.30957635737016886 1103 14-15 0.3367575709398025 1103 16-17 0.2554990203551313 1103 18-19 0.23319836575047503 1103 20-21 0.21859252518284933 1103 22-23 0.21789268809042284 1103 24-25 0.20460632051833727 1103 26-27 0.1935788863229888 1103 28-29 0.18401484304306592 1103 30-31 0.09098068472865606 1103 32-33 0.09602220043385046 1103 34-35 -0.035638787393921234 1103 36-37 0.16268016385281925 1103 38-39 0.28754705234158706 1103 40-41 0.32680576586267307 1103 42-43 0.20096793551178393 1103 44-45 0.16153518759248442 1103 46-47 0.19974328642624073 1103 48-49 0.15484449486120155 1103 50-51 0.20862906899078126 1103 52-53 0.22486387690542387 1103 54-55 0.20444002407241157 1103 56-57 0.2634966111423793 1103 58-59 0.11408299195341698 1103 60-61 0.10389092088921359 1103 62-63 0.09591317773027441 1103 64-65 0.05601088353289896 1103 66-67 0.2579256703798549 1103 68-69 -4.022256971034256 1103 70-71 0.4018910282286221 1103 72-73 0.6846759830798099 1103 74-75 0.4059894008804399 1103 76-77 0.2910666259232464 1103 78-79 0.06700135917629879 1103 80-81 0.18492281096506602 1103 82-83 0.08391741809529307 1103 84-85 0.008251084729337776 1103 86-87 -0.1508075333213732 1103 88-89 -0.2571466542432006 1103 90-91 -0.31219751610042223 1103 92-93 -0.335857460513143 1103 94-95 -0.19523037877462457 1103 96-97 -0.5493127585380186 1103 98-99 -0.15973567388211762 1103 100-101 -0.32799859155433353 1104 1 0.1439841328159872 1104 2 0.2457019767739652 1104 3 0.21980870365920424 1104 4 0.18793603923491276 1104 5 0.25149258412582753 1104 6 0.2224472169692575 1104 7 0.15415557397824386 1104 8 0.18117385118706153 1104 9 0.1979270861457323 1104 10-11 0.2078758514151815 1104 12-13 0.2318919964695354 1104 14-15 0.32244044106161596 1104 16-17 0.30364434338994073 1104 18-19 0.23159273162709582 1104 20-21 0.3821680329828254 1104 22-23 0.3533990123368369 1104 24-25 0.30592439879028177 1104 26-27 0.32703263647970005 1104 28-29 0.29451356839231124 1104 30-31 0.31805365102791683 1104 32-33 0.2709573941387333 1104 34-35 0.2333755714014032 1104 36-37 0.32515380861979537 1104 38-39 0.31270812051158714 1104 40-41 0.19561296293603192 1104 42-43 0.22554545793725111 1104 44-45 0.20119174864365874 1104 46-47 0.28902991517550447 1104 48-49 0.2928429912340107 1104 50-51 0.3570948397217748 1104 52-53 0.268115987388974 1104 54-55 0.16980151131975418 1104 56-57 0.24578528425195145 1104 58-59 0.12218717448553207 1104 60-61 0.06736427032707581 1104 62-63 0.23736992945136137 1104 64-65 0.27883060116595715 1104 66-67 0.34710363857058013 1104 68-69 -3.905728364765608 1104 70-71 0.8049477993324032 1104 72-73 1.0472373547284697 1104 74-75 0.8071662020714498 1104 76-77 0.6771397471372147 1104 78-79 0.3402217717964149 1104 80-81 0.2557311106127109 1104 82-83 0.12491002679118068 1104 84-85 0.050789949692713776 1104 86-87 0.10620196750266331 1104 88-89 0.07988632331857559 1104 90-91 0.016990930859360986 1104 92-93 -0.12656407810635173 1104 94-95 0.2738338959647848 1104 96-97 -0.08038194631319229 1104 98-99 0.20039900647504538 1104 100-101 0.2050431662210883 1105 1 0.15826890139354433 1105 2 0.20324777195767396 1105 3 0.15427492517172325 1105 4 0.11424224789703175 1105 5 0.2416718196878449 1105 6 0.1907732683497727 1105 7 0.1025068651736305 1105 8 0.2874225546671241 1105 9 0.23618744539588477 1105 10-11 0.14808256292603517 1105 12-13 0.21306822437330908 1105 14-15 0.2636654958393052 1105 16-17 0.1834928142691865 1105 18-19 0.1955610912696315 1105 20-21 0.2635188142360434 1105 22-23 0.17579967858503665 1105 24-25 0.23612056929250258 1105 26-27 0.22545243217049205 1105 28-29 0.2711870417328228 1105 30-31 0.4042131744839139 1105 32-33 0.34917442081135164 1105 34-35 0.3650921700549361 1105 36-37 0.1598704985769146 1105 38-39 0.3796941353385037 1105 40-41 0.3347224277236691 1105 42-43 0.3293988715391265 1105 44-45 0.23336130066464023 1105 46-47 0.21850204462005962 1105 48-49 0.25918167074107146 1105 50-51 0.2134747586134509 1105 52-53 0.17523939625414897 1105 54-55 0.2969122591819371 1105 56-57 0.26708812307895613 1105 58-59 0.300071117256941 1105 60-61 0.16836328528459887 1105 62-63 0.19338274894022334 1105 64-65 0.1288248299212924 1105 66-67 0.7371435155483681 1105 68-69 2.0649619091505294 1105 70-71 2.2574303323834997 1105 72-73 1.3271909632125904 1105 74-75 0.7370531732275545 1105 76-77 0.40700650106939307 1105 78-79 0.39017819264558184 1105 80-81 0.4320560132832618 1105 82-83 0.3823085658979757 1105 84-85 0.2590871234087828 1105 86-87 0.29879976999852076 1105 88-89 0.4299374604771202 1105 90-91 0.49025398722834623 1105 92-93 0.4158826446396624 1105 94-95 0.7185068227344829 1105 96-97 0.2782463458647335 1105 98-99 0.6418149768848203 1105 100-101 0.6482843597573691 1106 1 0.39451842093508205 1106 2 0.3423801466304113 1106 3 0.3484518658503575 1106 4 0.22548002131721034 1106 5 0.32100084233891835 1106 6 0.35087632968428295 1106 7 0.30736515109516915 1106 8 0.35695005866388385 1106 9 0.405959064745943 1106 10-11 0.30279528706942216 1106 12-13 0.42664268970717956 1106 14-15 0.5552148297624768 1106 16-17 0.5269833901206198 1106 18-19 0.551519252925381 1106 20-21 0.5053795763182265 1106 22-23 0.5094021666898527 1106 24-25 0.6556797148854443 1106 26-27 0.6679238787191579 1106 28-29 0.6103646268175495 1106 30-31 0.6496500227579887 1106 32-33 0.5816549118141694 1106 34-35 0.5559043479971635 1106 36-37 0.7768717812699677 1106 38-39 0.8169412676128047 1106 40-41 0.6520927366690685 1106 42-43 0.7299956148241051 1106 44-45 0.785378415065864 1106 46-47 0.7736333931762971 1106 48-49 0.7622724956648312 1106 50-51 0.826265866160405 1106 52-53 0.8588643946947201 1106 54-55 0.7604230072921112 1106 56-57 0.7763962453422195 1106 58-59 0.9407354091001849 1106 60-61 1.0207049223094558 1106 62-63 0.9957465310504219 1106 64-65 0.9072838260793858 1106 66-67 1.4333214592900347 1106 68-69 2.9428319651007264 1106 70-71 3.3227437715543893 1106 72-73 2.2700915552835177 1106 74-75 1.7036026387831846 1106 76-77 1.2084222448897712 1106 78-79 1.1345149730732054 1106 80-81 1.1544466275429137 1106 82-83 1.2156082282026226 1106 84-85 1.173812677803184 1106 86-87 1.1843049352797905 1106 88-89 1.4053687725984503 1106 90-91 1.2312813379859548 1106 92-93 1.107926024815015 1106 94-95 1.433630400469518 1106 96-97 1.2251192971122933 1106 98-99 1.554337430891369 1106 100-101 1.5053995588375813 1107 1 0.09403709833925333 1107 2 0.08731686099606506 1107 3 0.1060607831475302 1107 4 0.09064383335737602 1107 5 0.08634426245946969 1107 6 0.07779782689183179 1107 7 0.036468183413028044 1107 8 0.09160369098000132 1107 9 0.03514965419095972 1107 10-11 0.08505617445027269 1107 12-13 0.08618011071489917 1107 14-15 0.14380802543333004 1107 16-17 0.09428619088563295 1107 18-19 0.06650802319229143 1107 20-21 0.12200784412706156 1107 22-23 0.19701029635944423 1107 24-25 0.20667956702406798 1107 26-27 0.14930363794671564 1107 28-29 0.17742881991483728 1107 30-31 0.12350873590015965 1107 32-33 0.09356857623457415 1107 34-35 0.25644433193536287 1107 36-37 0.17611319443813755 1107 38-39 0.29457489128918013 1107 40-41 0.23357331705879147 1107 42-43 0.16595414792330843 1107 44-45 0.10858690056916487 1107 46-47 0.11285702915513696 1107 48-49 0.23281430612587428 1107 50-51 0.19885251244216562 1107 52-53 0.3159359373720534 1107 54-55 0.2158239097296999 1107 56-57 0.12005514268293638 1107 58-59 0.10271988752160155 1107 60-61 0.16377317144717551 1107 62-63 0.23852734444260904 1107 64-65 0.12074070902632883 1107 66-67 0.7388551228793148 1107 68-69 2.072684089230833 1107 70-71 2.1898824751870407 1107 72-73 1.161690299138332 1107 74-75 0.5489271510097744 1107 76-77 0.33168468046618216 1107 78-79 0.3228569976916411 1107 80-81 0.38415557740824013 1107 82-83 0.4880432924160125 1107 84-85 0.45316393361012075 1107 86-87 0.46200524153645617 1107 88-89 0.4480554089261517 1107 90-91 0.399858404203691 1107 92-93 0.42268348384147103 1107 94-95 0.7412484503809473 1107 96-97 0.44049666253186714 1107 98-99 0.6714568062398847 1107 100-101 0.8283977767697834 1108 1 -0.13774455607499547 1108 2 -0.152005096786489 1108 3 -0.07958864372405827 1108 4 -0.09930245924389425 1108 5 -0.07738827396462256 1108 6 -0.010967614898078182 1108 7 -0.08358492155737451 1108 8 -0.09468732206713071 1108 9 -0.11271428098044822 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-0.6792346502692723 2316 58-59 -0.7343521067534695 2316 60-61 -0.7928651356315086 2316 62-63 -0.693682253314762 2316 64-65 -0.6076704390469665 2316 66-67 -0.2397640068061655 2316 68-69 1.100302558774633 2316 70-71 1.3980760446304323 2316 72-73 0.4098310387558932 2316 74-75 -0.07537424527309255 2316 76-77 -0.4637313567236454 2316 78-79 -0.6225139205709844 2316 80-81 -0.6498057072237735 2316 82-83 -0.50006352479501 2316 84-85 -0.7168415953075957 2316 86-87 -0.6389848322995597 2316 88-89 -0.7208444751329992 2316 90-91 -0.7328589663983713 2316 92-93 -0.7056150980558549 2316 94-95 -0.3595090483292793 2316 96-97 -0.45727545175215667 2316 98-99 -0.559223648203865 2316 100-101 -0.6737936809598821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 25.0 4 68.0 5 178.0 6 420.0 7 1168.0 8 2586.0 9 4524.0 10 7504.0 11 11786.0 12 16607.0 13 20720.0 14 25233.0 15 30916.0 16 37810.0 17 43652.0 18 40297.0 19 34456.0 20 33519.0 21 34555.0 22 38278.0 23 43448.0 24 52177.0 25 63694.0 26 79538.0 27 98592.0 28 120685.0 29 147158.0 30 175632.0 31 209079.0 32 249790.0 33 303271.0 34 376762.0 35 477015.0 36 604333.0 37 689908.0 38 431751.0 39 37684.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.936234681934884 20.875506017186428 14.77774858059896 23.410510720279728 2 15.00622948676556 21.818367489319858 40.16133941558368 23.014063608330897 3 17.91096072764942 26.327410554331454 31.362608821547845 24.399019896471284 4 12.28426856103937 17.93704449606355 38.89380656565957 30.88488037723752 5 12.01690976712215 39.07988617393526 35.861899314583674 13.04130474435892 6 30.088419246065577 39.03182190733416 17.774809811158846 13.10494903544141 7 26.699882437821092 33.969418871425084 22.392657677549586 16.938041013204234 8 25.3486984878859 36.261166261700936 20.71510281244905 17.675032437964113 9 25.94027190021891 16.579237634683285 21.009261294539357 36.47122917055845 10-11 23.50807918185701 27.480659888413843 29.072490956205392 19.93876997352376 12-13 24.797005563905707 24.939211134236295 29.02239006132024 21.24139324053776 14-15 21.51870840465065 26.639385112179138 26.905269703216554 24.93663677995366 16-17 20.70294807282098 29.844428262395194 28.128220358291696 21.324403306492133 18-19 20.788262880737648 28.235484767413695 30.393629771329127 20.58262258051953 20-21 21.280085565375508 27.646375694223043 30.410990160465882 20.66254857993557 22-23 21.01890962234223 27.56424939200133 30.380416952981072 21.036424032675377 24-25 20.914829403241114 27.859166430042254 30.376765840624685 20.84923832609195 26-27 20.504207431059914 28.10988387648697 30.144135648175858 21.24177304427726 28-29 20.70830048918302 28.21427019427051 30.218979949161927 20.858449367384534 30-31 20.857720156908858 27.989490855931297 29.94032305903621 21.212465928123635 32-33 20.335736965886888 28.262399530355953 29.981702937156612 21.42016056660055 34-35 21.16358079361709 28.186201972215592 29.76171903794254 20.88849819622478 36-37 21.321758459303037 27.528176752502485 29.82308627038714 21.326978517807337 38-39 20.7482112244017 28.02027117503308 30.005356195832388 21.22616140473283 40-41 20.996229959165326 27.806355601863515 30.23620545329419 20.961208985676972 42-43 20.788256601063967 27.854119391002257 29.744922036108374 21.612701971825402 44-45 20.821043580608446 27.93631485377397 30.057982084271583 21.184659481346007 46-47 21.236558577365543 28.087809421265497 29.315655636624033 21.359976364744927 48-49 20.767259506834737 28.417313640315832 29.210507885693342 21.604918967156085 50-51 21.00642923165253 28.292043878135086 29.261339798756435 21.440187091455947 52-53 21.171600787439456 28.01132783690522 29.376421800856555 21.44064957479877 54-55 20.728965193067893 28.306163179441906 29.394249959544744 21.570621667945456 56-57 20.887413217324124 28.40305569663577 28.907554886930093 21.801976199110012 58-59 20.54339913454361 27.980738930045206 29.240841936256214 22.235019999154964 60-61 20.37855685102807 28.408091555868616 29.33971831147079 21.873633281632525 62-63 20.536913962603666 28.267624372339036 29.586178603203848 21.60928306185345 64-65 20.196029871407106 28.069669730990327 29.39351168146642 22.34078871613615 66-67 20.093063001909275 28.329501156704385 29.184163401998873 22.393272439387466 68-69 20.44332303488452 27.905706890121795 29.521084571592187 22.129885503401493 70-71 20.3587267629714 27.675272758799878 29.296412898038433 22.66958758019029 72-73 20.055428551842844 27.94006125199933 29.165718409422965 22.83879178673486 74-75 20.28578619375387 28.252428220833647 28.64558314771108 22.816202437701406 76-77 20.107435896504718 27.933795294702584 29.114273976832823 22.844494831959874 78-79 20.168480959360966 27.972112449290186 28.87304814045038 22.986358450898468 80-81 20.399648431015038 28.117047520231775 28.400901299834896 23.08240274891829 82-83 20.319592028340804 27.9559248204359 28.543648155468237 23.180834995755063 84-85 20.016590370763982 27.920218799266415 28.532511295864175 23.530679534105424 86-87 20.088255242699166 27.983380906304987 28.44077453821946 23.487589312776382 88-89 20.010594484817045 27.852240491504887 28.360742758097544 23.77642226558052 90-91 19.81225132288579 27.752283434316897 28.49276722688946 23.942698015907855 92-93 20.059787877574355 27.534141712144212 28.478641434240153 23.92742897604128 94-95 19.440027604242015 27.144343805813758 28.986810254526702 24.428818335417525 96-97 19.74492205432056 26.686024530475155 29.024097822608937 24.544955592595347 98-99 19.563463275124697 27.029917997927143 28.611816471959926 24.794802254988234 100-101 19.26604878748656 26.88283855907606 28.91705150249914 24.934061150938238 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1016.0 1 674.5 2 421.5 3 583.0 4 562.0 5 564.0 6 908.0 7 1763.0 8 2656.0 9 3092.5 10 2950.5 11 2771.5 12 3006.5 13 3271.0 14 3527.0 15 3902.0 16 4304.5 17 4809.5 18 5418.0 19 6180.5 20 7376.0 21 9094.0 22 11203.5 23 13939.0 24 17452.5 25 22278.5 26 29498.0 27 38073.5 28 45653.5 29 54167.0 30 64755.5 31 79373.5 32 101214.5 33 115261.5 34 119935.0 35 130526.0 36 148683.0 37 163446.5 38 167968.5 39 168952.0 40 170898.5 41 172657.5 42 170391.0 43 172210.0 44 176181.5 45 178058.5 46 174142.5 47 167850.0 48 166544.0 49 159416.0 50 149977.0 51 141353.5 52 131656.0 53 125811.5 54 119240.0 55 107251.0 56 94348.5 57 82035.0 58 73370.0 59 62564.5 60 47396.0 61 36957.0 62 30523.0 63 21877.5 64 13616.0 65 9582.5 66 7335.5 67 6053.5 68 4684.5 69 3160.5 70 2145.0 71 1444.5 72 966.0 73 759.0 74 559.0 75 342.5 76 242.5 77 190.0 78 129.0 79 80.0 80 42.5 81 25.0 82 18.5 83 8.0 84 4.5 85 4.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022245061365345098 2 0.024555379311300824 3 6.60090841701635E-5 4 0.0 5 0.0 6 8.801211222688467E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.750378507090146E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.701059819852408E-5 26-27 2.8603936473737515E-4 28-29 1.4301968236868758E-4 30-31 5.500757014180291E-4 32-33 0.0024313346002676887 34-35 0.004268587443003906 36-37 0.10231408046375343 38-39 0.09445899944750397 40-41 0.10324920915616408 42-43 0.10443737267122702 44-45 0.09815550816103312 46-47 0.09141158006164808 48-49 0.09243472086628561 50-51 0.0915766027720735 52-53 0.07202691234367674 54-55 0.061036399829344515 56-57 0.033257576907734045 58-59 0.014015928872131383 60-61 0.00907624907339748 62-63 0.0017272377024526114 64-65 0.004741652546223411 66-67 0.1478713500551946 68-69 0.40883826432193593 70-71 0.38959661628633335 72-73 0.17596921688362752 74-75 0.029209019745297347 76-77 0.030265165092019965 78-79 0.0048516676865070175 80-81 0.0013091801693749093 82-83 7.701059819852408E-5 84-85 5.28072673361308E-4 86-87 0.001243171085204746 88-89 2.530348226522934E-4 90-91 6.600908417016349E-4 92-93 0.002090287665388511 94-95 0.07696659214241064 96-97 0.016876322519505136 98-99 0.01888959958669512 100-101 0.010275414102488783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4544829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.17255136372322 #Duplication Level Percentage of deduplicated Percentage of total 1 65.06860909444578 22.235603864459762 2 14.262684152002192 9.74784613537712 3 6.631026022653688 6.797972320599555 4 3.7755107611589747 5.160753416399793 5 2.3874145757309306 4.079202360783335 6 1.6546320959301888 3.3925800171743634 7 1.1467040730265787 2.743006268414397 8 0.8755849592223995 2.3936777593864758 9 0.624737231310221 1.9213978613200886 >10 3.2164871365745378 19.081286352035246 >50 0.19229224345237256 4.55309335845974 >100 0.1456761056023878 9.933690054552814 >500 0.011997675228569229 2.79453067109565 >1k 0.006256851894388861 4.039823620735716 >5k 2.5801451110881737E-4 0.5696672831623335 >10k+ 1.2900725555440868E-4 0.5558686560434811 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13953 0.30700825047543046 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11265 0.24786411105896392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7852 0.17276777630137458 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6549 0.1440978307434669 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5899 0.12979586250659814 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5544 0.12198478754646214 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4791 0.10541650741975112 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4791 0.10541650741975112 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4694 0.10328221369824916 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4006056113442335E-5 2 0.0 0.0 0.0 0.0 4.4006056113442335E-5 3 0.0 0.0 0.0 0.0 4.4006056113442335E-5 4 0.0 0.0 0.0 0.0 4.4006056113442335E-5 5 0.0 0.0 0.0 0.0 4.4006056113442335E-5 6 0.0 0.0 0.0 0.0 6.60090841701635E-5 7 0.0 0.0 0.0 2.2003028056721167E-5 6.60090841701635E-5 8 0.0 0.0 0.0 2.2003028056721167E-5 6.60090841701635E-5 9 0.0 0.0 0.0 1.32018168340327E-4 6.60090841701635E-5 10-11 0.0 0.0 0.0 1.5402119639704816E-4 6.60090841701635E-5 12-13 0.0 0.0 0.0 1.8702573848212992E-4 1.5402119639704816E-4 14-15 0.0 0.0 0.0 1.980272525104905E-4 2.2003028056721168E-4 16-17 0.0 0.0 0.0 2.8603936473737515E-4 2.64036336680654E-4 18-19 0.0 0.0 0.0 3.520484489075386E-4 2.9704087876573573E-4 20-21 0.0 0.0 0.0 5.28072673361308E-4 3.520484489075387E-4 22-23 0.0 0.0 0.0 0.0011111529168644188 3.520484489075387E-4 24-25 0.0 0.0 0.0 0.0022883149178990012 3.520484489075387E-4 26-27 0.0 0.0 0.0 0.004466614695514397 3.850529909926204E-4 28-29 0.0 0.0 0.0 0.01166160487006222 3.96054505020981E-4 30-31 0.0 0.0 0.0 0.03579892664828534 3.96054505020981E-4 32-33 0.0 0.0 0.0 0.09400793737234117 3.96054505020981E-4 34-35 0.0 0.0 0.0 0.18165699963628995 4.1805753307770215E-4 36-37 0.0 0.0 0.0 0.3239945881352192 4.1805753307770215E-4 38-39 0.0 0.0 0.0 0.5455210746102879 4.1805753307770215E-4 40-41 0.0 0.0 0.0 0.7872463408414265 4.1805753307770215E-4 42-43 0.0 0.0 0.0 1.0362766123873968 4.1805753307770215E-4 44-45 0.0 0.0 0.0 1.3297750036360003 4.400605611344233E-4 46-47 0.0 0.0 0.0 1.6726591913579147 4.7306510321950504E-4 48-49 0.0 0.0 0.0 2.0253567295931267 5.060696453045868E-4 50-51 0.0 0.0 0.0 2.40897952376206 5.720787294747503E-4 52-53 0.0 0.0 0.0 2.768211961330118 6.820938697583561E-4 54-55 0.0 0.0 0.0 3.1530449220421715 7.040968978150774E-4 56-57 0.0 0.0 0.0 3.5772302984336704 7.260999258717985E-4 58-59 0.0 0.0 0.0 4.011086005656098 7.260999258717985E-4 60-61 0.0 0.0 0.0 4.461399977864954 7.481029539285197E-4 62-63 0.0 2.2003028056721167E-5 0.0 4.884694231620156 7.92109010041962E-4 64-65 0.0 2.2003028056721167E-5 0.0 5.323995688286622 8.141120380986831E-4 66-67 0.0 2.2003028056721167E-5 0.0 5.797192809674467 8.251135521270437E-4 68-69 0.0 2.2003028056721167E-5 0.0 6.321987031855324 8.361150661554043E-4 70-71 0.0 2.2003028056721167E-5 0.0 6.90550733591957 8.69119608240486E-4 72-73 0.0 2.2003028056721167E-5 0.0 7.505331883773845 9.24127178382289E-4 74-75 0.0 2.2003028056721167E-5 0.0 8.103583215122065 9.791347485240919E-4 76-77 0.0 2.2003028056721167E-5 0.0 8.738898647231832 0.0010231408046375342 78-79 0.0 2.2003028056721167E-5 0.0 9.441081281605975 0.0010341423186658947 80-81 0.0 2.2003028056721167E-5 0.0 10.175982418700462 0.0010451438326942552 82-83 0.0 2.2003028056721167E-5 0.0 10.91054250886007 0.001056145346722616 84-85 0.0 2.2003028056721167E-5 0.0 11.631779765531332 0.001056145346722616 86-87 0.0 2.2003028056721167E-5 0.0 12.35829774893621 0.00113315594492114 88-89 0.0 3.300454208508175E-5 0.0 13.11054827365342 0.0012211680571480247 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10135 0.0 39.103203 1 GTATCAA 16270 0.0 34.831036 1 ATCAACG 20540 0.0 27.369493 3 CAACGCA 20875 0.0 26.907547 5 TCAACGC 20935 0.0 26.853088 4 TATCAAC 21140 0.0 26.598835 2 GTACATG 17285 0.0 26.195616 1 AACGCAG 21615 0.0 25.986355 6 TACATGG 17390 0.0 25.649418 2 ACATGGG 17105 0.0 25.100039 3 TAAGGTG 2850 0.0 22.638252 5 ACGCAGA 24795 0.0 22.634428 7 CGCAGAG 25025 0.0 22.369526 8 CATGGGA 9580 0.0 22.13558 4 GTATAGG 1560 0.0 21.297226 1 GGTAAGG 2450 0.0 20.52526 3 ATGGGAT 4885 0.0 20.296942 5 CATGGGG 6600 0.0 20.126257 4 GCAGAGT 27350 0.0 20.034271 9 AGGTAAG 2400 0.0 19.771425 2 >>END_MODULE