FastQCFastQC Report
Fri 27 May 2016
SRR939867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009405
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184771.83048429520361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132551.3131498258875278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98340.9742372982103318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78810.7807569805974807No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37730.373784556248483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34410.3408938929369282No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.2727349280021399No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.26362064780737166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.24786879399250053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22370.22161570430104865No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20900.20705266964201682No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17150.16990207102203775No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.1589054938305239No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14010.13879463644424192No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10110.10015801387946363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA651.3364307E-436.49952
GTATCAA169650.031.6344661
TACATGG124500.031.2136672
GTACATG122250.030.8969761
GGTATCA111250.030.113171
ACATGGG126050.029.2879183
ATATCAA7600.028.7207661
CATGGGA93850.027.7102974
TATCAAC207500.027.0061112
CAACGCG1257.442597E-626.575595
ATGGGAG28800.026.5294515
ATCAACG213950.026.2154313
TCAACGC214700.026.1264444
CAACGCA215700.025.8953135
AACGCAG218600.025.5300736
GTACACG1351.3531388E-524.6045881
CATGGGC10500.024.4061554
GAGTACT118850.024.29539512-13
ATGGGAT34650.024.2418025
AGTACTT119650.023.91482412-13
Produced by FastQC (version 0.11.5)