FastQCFastQC Report
Fri 27 May 2016
SRR939866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105373
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199361.8035540944097606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146151.3221781244882949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110641.0009290981415322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87300.7897786539023479No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42190.38168111578625497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.3384377943011092No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.27710103286401966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.2688685176858852No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.2526748889288955No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.22870108099256994No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23270.2105171738408664No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.16211722196941666No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17650.15967460757590424No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16180.14637592921122555No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12370.11190792610277255No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.10105186213160626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTG1703.6379788E-1233.1943661
GTACATG132100.032.9284251
GTATCAA175650.032.0730861
TACATGG133000.032.0352632
ATGGGAG29350.030.4464455
ACATGGG136700.030.2737273
GGTATCA116050.030.0265271
GATGCGA650.006136512428.9399912
CATGGGA99950.028.2318864
GTGTAAG2850.028.050211
CATGGGG31550.028.0239854
CGTCACT2850.026.40021
TATCAAC215300.026.2768542
CATGGGT8600.026.2490884
ATCAACG221200.025.5547183
TCAACGC222700.025.2570364
ATGGGAT35650.025.1979355
ATGGGCG1501.1556403E-625.0835975
CAACGCA224750.024.9440885
AACGCAG229700.024.52946
Produced by FastQC (version 0.11.5)