##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939866_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105373 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.36147255270393 31.0 30.0 33.0 27.0 34.0 2 30.298207030567962 31.0 30.0 33.0 26.0 34.0 3 30.191064011876534 31.0 30.0 34.0 26.0 34.0 4 33.89817011995046 35.0 35.0 37.0 28.0 37.0 5 33.86123326696057 35.0 35.0 37.0 28.0 37.0 6 33.94701245642874 36.0 35.0 37.0 29.0 37.0 7 33.96383483222405 35.0 35.0 37.0 29.0 37.0 8 33.836334884242696 35.0 35.0 37.0 28.0 37.0 9 35.080422626570396 37.0 35.0 39.0 28.0 39.0 10-11 34.879038116545274 37.0 34.0 39.0 27.0 39.0 12-13 34.77706258430412 37.0 34.0 39.0 27.0 39.0 14-15 35.188157753084255 38.0 33.5 40.0 27.0 41.0 16-17 35.11363404027419 38.0 33.5 40.0 27.0 41.0 18-19 35.37847360121877 38.0 34.0 40.0 27.0 41.0 20-21 35.60786178059352 38.0 34.0 40.0 27.0 41.0 22-23 35.50582699233652 38.0 34.0 40.0 27.0 41.0 24-25 35.22969531551793 38.0 34.0 40.0 26.5 41.0 26-27 34.99173808298194 38.0 34.0 40.0 25.0 41.0 28-29 34.806817246305094 38.0 33.5 40.0 25.0 41.0 30-31 34.430677698840114 37.5 33.0 40.0 24.5 41.0 32-33 33.90077828931953 37.0 33.0 40.0 23.5 41.0 34-35 33.49580548828314 37.0 33.0 40.0 21.0 41.0 36-37 33.115968998699984 36.0 32.0 40.0 18.5 41.0 38-39 32.703505513523496 36.0 31.0 40.0 16.5 41.0 40-41 32.274353091671315 35.5 31.0 39.0 13.5 41.0 42-43 31.73420872411394 35.0 30.5 39.0 9.0 41.0 44-45 31.037566504700223 35.0 30.0 39.0 4.5 40.0 46-47 30.307934968558126 35.0 28.0 39.0 2.0 40.0 48-49 29.721421637763903 35.0 27.0 39.0 2.0 40.0 50-51 28.86620851061135 34.5 25.0 38.5 2.0 39.5 52-53 28.93554528652319 34.5 26.0 38.0 2.0 40.0 54-55 28.986595022675605 35.0 26.0 38.0 2.0 40.0 56-57 28.718776376842932 35.0 25.0 38.0 2.0 40.0 58-59 28.19117935755623 34.0 23.5 38.0 2.0 40.0 60-61 27.686115908385673 34.0 22.5 38.0 2.0 40.0 62-63 27.243292987977817 33.5 22.0 37.5 2.0 40.0 64-65 26.72638331133473 33.0 20.0 37.0 2.0 39.0 66-67 26.175522651629812 33.0 18.5 36.0 2.0 39.0 68-69 25.632660197055657 32.0 16.5 36.0 2.0 39.0 70-71 25.122487612778674 31.5 10.5 35.0 2.0 38.0 72-73 24.656293848320885 31.0 7.5 35.0 2.0 38.0 74-75 24.09428898661357 31.0 4.0 35.0 2.0 37.0 76-77 23.458837424109326 30.5 2.0 34.5 2.0 36.5 78-79 22.806585197937707 30.0 2.0 34.0 2.0 36.0 80-81 22.10106588454757 29.0 2.0 34.0 2.0 35.5 82-83 21.36250297410919 29.0 2.0 34.0 2.0 35.0 84-85 20.633286230077992 27.5 2.0 34.0 2.0 35.0 86-87 19.8741714335342 26.5 2.0 33.0 2.0 35.0 88-89 19.06106581217381 25.5 2.0 33.0 2.0 35.0 90-91 18.196496114886106 24.5 2.0 32.5 2.0 34.0 92-93 17.279330144666098 21.5 2.0 32.0 2.0 34.0 94-95 15.987453556401324 9.5 2.0 31.5 2.0 34.0 96-97 13.639820675916637 2.0 2.0 30.5 2.0 34.0 98-99 12.417259603771758 2.0 2.0 29.0 2.0 34.0 100-101 11.227152282532684 2.0 2.0 26.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7404428440828532 1101 2 0.713595218977094 1101 3 0.8123509840928378 1101 4 1.0073450508776602 1101 5 0.9394048894305769 1101 6 0.7959593440316013 1101 7 0.6249813547251861 1101 8 0.5836626656243595 1101 9 0.8669950941987068 1101 10-11 0.8731489047125152 1101 12-13 0.8336871964766601 1101 14-15 0.982016346120588 1101 16-17 1.0299540744241966 1101 18-19 0.8150401244588181 1101 20-21 0.977358844693768 1101 22-23 1.040960056276809 1101 24-25 1.045484335680996 1101 26-27 1.206054105253429 1101 28-29 1.4427392836790602 1101 30-31 1.233292074629425 1101 32-33 1.0831484621720122 1101 34-35 1.0289178344830603 1101 36-37 1.115857762128286 1101 38-39 1.1491803325741472 1101 40-41 1.3898895014869765 1101 42-43 1.2782130669257405 1101 44-45 1.0203595309377747 1101 46-47 0.9198376152665624 1101 48-49 0.7822966591108624 1101 50-51 0.6934915612723813 1101 52-53 0.9508499162973223 1101 54-55 0.8373062032568939 1101 56-57 0.2606517444632672 1101 58-59 0.2018457660299262 1101 60-61 0.4387853655224454 1101 62-63 0.5214609793290315 1101 64-65 0.274762010567251 1101 66-67 -0.08170247249580953 1101 68-69 0.23487079131732713 1101 70-71 0.1833977768261299 1101 72-73 -0.13327106925855858 1101 74-75 -0.14784860831658264 1101 76-77 -0.6896439493167179 1101 78-79 -0.5423153194854571 1101 80-81 -1.0449739726058986 1101 82-83 -0.8226669208925479 1101 84-85 -0.82870091997626 1101 86-87 -0.8235101770117588 1101 88-89 -1.0877187220097646 1101 90-91 -1.188936526557626 1101 92-93 -1.3266120948374684 1101 94-95 -0.6435441026865014 1101 96-97 0.8129549981931525 1101 98-99 0.8260080847065083 1101 100-101 0.3441495307613227 1102 1 0.7087823914841209 1102 2 0.6357032930928952 1102 3 0.7033246406173106 1102 4 0.8191072234469416 1102 5 0.8683409873535552 1102 6 0.8992708312651914 1102 7 0.7346469872040515 1102 8 0.8067626063150257 1102 9 1.0551181774493301 1102 10-11 0.7952916256099627 1102 12-13 0.7475079839495749 1102 14-15 0.8752435336364215 1102 16-17 0.9897213374625622 1102 18-19 0.8442499692114325 1102 20-21 0.9722337288319522 1102 22-23 0.7229696590235122 1102 24-25 0.7908859999186646 1102 26-27 0.872740144314136 1102 28-29 0.939110685256864 1102 30-31 0.8681727406821338 1102 32-33 1.2346446815748138 1102 34-35 1.216048827631191 1102 36-37 1.098043355623929 1102 38-39 1.0834538976013093 1102 40-41 1.3090216778413293 1102 42-43 1.1237249302818135 1102 44-45 0.6112551763216345 1102 46-47 0.48723082121895445 1102 48-49 0.21239919868270363 1102 50-51 0.39627358607500796 1102 52-53 0.3822112889357534 1102 54-55 0.3029939679508544 1102 56-57 0.07916445126357274 1102 58-59 0.0013034880152069661 1102 60-61 0.34130459634899424 1102 62-63 0.32774635257545626 1102 64-65 0.17601197703810811 1102 66-67 -0.10951303432504034 1102 68-69 -0.3946981133079035 1102 70-71 -0.07664643693781059 1102 72-73 0.0822032265549204 1102 74-75 0.19819674096768125 1102 76-77 -0.1328496781806905 1102 78-79 -0.3237712443086451 1102 80-81 -0.6429878551615076 1102 82-83 -0.4230017652726161 1102 84-85 -0.47882952286900604 1102 86-87 -0.6165783618780409 1102 88-89 -0.48485950566781 1102 90-91 -0.5768359331662936 1102 92-93 -0.5758420421743615 1102 94-95 -0.31753651466877386 1102 96-97 0.7934559384993545 1102 98-99 0.6926660337958559 1102 100-101 0.41347438792717206 1103 1 0.5850148095850507 1103 2 0.7144162756121055 1103 3 0.8590684354614524 1103 4 0.8361558610838173 1103 5 0.8204613007195434 1103 6 1.1224647077462677 1103 7 0.8798109351361632 1103 8 0.717242092227039 1103 9 0.9703408440560821 1103 10-11 0.9157431752137626 1103 12-13 0.6791003535177254 1103 14-15 0.805552930122289 1103 16-17 0.8659619941530465 1103 18-19 0.746572502502822 1103 20-21 0.8254890951495994 1103 22-23 0.7127208536189968 1103 24-25 0.8181433734563157 1103 26-27 1.0695405757166512 1103 28-29 1.0145716717905202 1103 30-31 1.1397911741517817 1103 32-33 1.0013783804019312 1103 34-35 0.9287343831368133 1103 36-37 0.938444649831446 1103 38-39 1.1282489814102377 1103 40-41 1.346703488533521 1103 42-43 1.2828832460145243 1103 44-45 1.452905365809194 1103 46-47 1.2350651956104244 1103 48-49 0.9477843152961967 1103 50-51 1.3921056087236359 1103 52-53 1.5110096532012456 1103 54-55 1.329169464887599 1103 56-57 1.1148862266047246 1103 58-59 1.0027182785303452 1103 60-61 1.039975562061482 1103 62-63 1.0017891782618804 1103 64-65 0.9724364291719034 1103 66-67 1.0957184700414118 1103 68-69 0.8141452636150532 1103 70-71 1.1393862328145836 1103 72-73 1.177210266804174 1103 74-75 1.037808628503445 1103 76-77 0.8959707804840562 1103 78-79 0.7497750191165125 1103 80-81 0.5781733365879198 1103 82-83 0.8184720435022292 1103 84-85 0.6853610731555015 1103 86-87 0.5957589259782772 1103 88-89 0.40309193159158596 1103 90-91 0.40855779651809243 1103 92-93 0.5421049762051808 1103 94-95 0.40125046536388 1103 96-97 1.9228692011306112 1103 98-99 2.251227051785941 1103 100-101 1.6621299999045824 1104 1 0.383707182504228 1104 2 0.41894387859331417 1104 3 0.6190697743287714 1104 4 0.45868676660441565 1104 5 0.6298601492936484 1104 6 0.6936768289583881 1104 7 0.66908437647146 1104 8 0.7132656562918456 1104 9 0.707474329127713 1104 10-11 0.5947140325351299 1104 12-13 0.5371012014670242 1104 14-15 0.7257084618757617 1104 16-17 0.945938117685877 1104 18-19 0.7351776259017555 1104 20-21 0.600601783148889 1104 22-23 0.6179000386950904 1104 24-25 0.6604236876438492 1104 26-27 0.9514033864454348 1104 28-29 1.0492829227388825 1104 30-31 0.7867686811808383 1104 32-33 1.131449340369798 1104 34-35 0.9430981980160951 1104 36-37 0.777332051089985 1104 38-39 0.6217644006988579 1104 40-41 0.7345543833431805 1104 42-43 0.6979321146716337 1104 44-45 0.5344959401832696 1104 46-47 0.2999545159121162 1104 48-49 0.21973727642441432 1104 50-51 0.4899266021631874 1104 52-53 0.7223342392681005 1104 54-55 0.5243328067004249 1104 56-57 0.480737299930027 1104 58-59 0.329849309011891 1104 60-61 0.37676875168972046 1104 62-63 0.2636091452364866 1104 64-65 0.43979038106193613 1104 66-67 0.5405392849653197 1104 68-69 0.2692084135235646 1104 70-71 0.32615264509646025 1104 72-73 0.17123668711925433 1104 74-75 0.19990306327829188 1104 76-77 0.46225252279673157 1104 78-79 0.26938384671503 1104 80-81 0.27150577734304093 1104 82-83 0.2683917114631349 1104 84-85 -0.046802982719896136 1104 86-87 0.1256926520460233 1104 88-89 0.2211789133383597 1104 90-91 -0.16277928564372957 1104 92-93 -0.08901253171644896 1104 94-95 0.6458726816355806 1104 96-97 2.00326818359145 1104 98-99 2.150051079991412 1104 100-101 1.7055037224020158 1105 1 0.6141461572792721 1105 2 0.7435942832332252 1105 3 0.6917822286542865 1105 4 0.7891796835687188 1105 5 0.7955552686278864 1105 6 0.7707369943419025 1105 7 0.8581399913095282 1105 8 0.7333242136983813 1105 9 1.1234942774490335 1105 10-11 1.0257620984063394 1105 12-13 0.8998951276059088 1105 14-15 0.8332455967371075 1105 16-17 0.9865105075159306 1105 18-19 1.205351049305321 1105 20-21 1.2805063334003748 1105 22-23 0.929031090288511 1105 24-25 0.9497658428033375 1105 26-27 1.2333402490971608 1105 28-29 1.2662734630132633 1105 30-31 1.2014911508218162 1105 32-33 1.2838264712332617 1105 34-35 1.3824529200888875 1105 36-37 1.285275663129525 1105 38-39 1.300221918652639 1105 40-41 1.1128776337495765 1105 42-43 0.9806435695233482 1105 44-45 1.1160647613039174 1105 46-47 1.0135497848734545 1105 48-49 0.9332653013759824 1105 50-51 0.9134525252801353 1105 52-53 0.6662938899226098 1105 54-55 1.19133863304479 1105 56-57 0.7075969091357663 1105 58-59 0.8133282274636464 1105 60-61 0.7672716192976559 1105 62-63 0.8420722484850707 1105 64-65 0.7980708745668252 1105 66-67 0.6889890716952465 1105 68-69 0.7205428580366018 1105 70-71 0.9808980144461259 1105 72-73 0.6574549721985825 1105 74-75 0.5033528647063612 1105 76-77 0.3575359618097167 1105 78-79 -0.10973971764373402 1105 80-81 -0.029844394184301848 1105 82-83 -0.16785141347990873 1105 84-85 -0.166016690797111 1105 86-87 -0.23612017296668952 1105 88-89 -0.32668228620658724 1105 90-91 -0.29042605396276144 1105 92-93 -0.15623081973540565 1105 94-95 -0.21196466282130544 1105 96-97 1.1962445283981538 1105 98-99 1.482406251827415 1105 100-101 0.9754384479435689 1106 1 0.9197691077248145 1106 2 1.104913175216641 1106 3 0.9480364438406852 1106 4 0.8547016820215418 1106 5 0.9366283596648941 1106 6 0.947510008964521 1106 7 0.7538197843060033 1106 8 1.0106840688336902 1106 9 1.3390836008393165 1106 10-11 1.1838714284386143 1106 12-13 1.0835281244588373 1106 14-15 1.1328399944838665 1106 16-17 1.2371906428425987 1106 18-19 0.8834351492090775 1106 20-21 0.7640335620289349 1106 22-23 0.7061540686713457 1106 24-25 0.9125754056988313 1106 26-27 1.0871011200481178 1106 28-29 1.165295893903881 1106 30-31 1.0173273454322 1106 32-33 1.3292556549213401 1106 34-35 1.2509847485334546 1106 36-37 1.343012207388881 1106 38-39 1.059023542635579 1106 40-41 1.0804585712306434 1106 42-43 1.104977894696109 1106 44-45 1.2430968057706409 1106 46-47 1.4128614257525598 1106 48-49 1.283711865207188 1106 50-51 1.2388062861510853 1106 52-53 1.3845899827153367 1106 54-55 1.404701629748189 1106 56-57 1.4359693140171572 1106 58-59 1.1933473979543123 1106 60-61 1.6021901643864815 1106 62-63 1.3864795568804986 1106 64-65 1.5822187045475538 1106 66-67 1.2947415567369944 1106 68-69 1.286325735703528 1106 70-71 1.220247413951757 1106 72-73 1.291114056972912 1106 74-75 1.0232835644843625 1106 76-77 1.2883522107679717 1106 78-79 1.1847563211631318 1106 80-81 1.2967004396596415 1106 82-83 1.309983690395665 1106 84-85 1.4311700633067161 1106 86-87 1.5680767951682704 1106 88-89 1.6713408553142877 1106 90-91 1.5983740826545265 1106 92-93 1.744569760730684 1106 94-95 1.8846055034567328 1106 96-97 3.099426467844271 1106 98-99 3.3191796756723253 1106 100-101 2.477512103235169 1107 1 0.5601667733380502 1107 2 0.6470789497255574 1107 3 0.763973892698516 1107 4 0.361091374620365 1107 5 0.7116933846977389 1107 6 0.6181155163504428 1107 7 0.5771955884793627 1107 8 0.4758406611883075 1107 9 0.6396049133201487 1107 10-11 0.7963770491118893 1107 12-13 0.9258513572635465 1107 14-15 0.8929337703528688 1107 16-17 0.8743362623718767 1107 18-19 0.9298587326802448 1107 20-21 0.7554635248649646 1107 22-23 0.6523088131551447 1107 24-25 0.5201912392347481 1107 26-27 0.6450985238134521 1107 28-29 0.6668180437260105 1107 30-31 0.6501774200302535 1107 32-33 0.5104177003220869 1107 34-35 0.39152064695934996 1107 36-37 0.39741555802515194 1107 38-39 0.5295017010567378 1107 40-41 0.5257358551306481 1107 42-43 0.3558875301587001 1107 44-45 0.3066680107635449 1107 46-47 -0.016652948076632867 1107 48-49 0.024368439289645494 1107 50-51 0.14336169288127465 1107 52-53 0.057492617559855574 1107 54-55 0.08497548960554013 1107 56-57 0.19788871634296612 1107 58-59 0.17637415633129905 1107 60-61 0.35000068374463567 1107 62-63 -0.05657051040868666 1107 64-65 0.07217736181438994 1107 66-67 0.1887729975519008 1107 68-69 0.028507240153196278 1107 70-71 0.07315022357339629 1107 72-73 0.08439125507324619 1107 74-75 0.19128529001119432 1107 76-77 0.12010778337753791 1107 78-79 0.0018834460954053611 1107 80-81 -0.11136639828860595 1107 82-83 -0.09209027845905382 1107 84-85 -0.29988330250338535 1107 86-87 0.06865554576608801 1107 88-89 0.034425623806104255 1107 90-91 0.006705072385578603 1107 92-93 0.0982014703845806 1107 94-95 0.24386424191548173 1107 96-97 1.8258871676200794 1107 98-99 1.923429460775914 1107 100-101 1.76503357301541 1108 1 0.5283950404652842 1108 2 0.35675709451542303 1108 3 0.3010124289679439 1108 4 0.35536130841426683 1108 5 0.42458738297062837 1108 6 0.4636421067361667 1108 7 0.5199399033901315 1108 8 0.39219248905244086 1108 9 0.6682507466534844 1108 10-11 0.6685434998890827 1108 12-13 0.46665446607183014 1108 14-15 0.6317794813488362 1108 16-17 0.5537246655094705 1108 18-19 0.5523490176543362 1108 20-21 0.5855495208350234 1108 22-23 0.37197184568248787 1108 24-25 0.6089667861547241 1108 26-27 0.43499534664016437 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0.4724836009272444 2311 60-61 0.24648587659595478 2311 62-63 0.4023292766449451 2311 64-65 0.07669297216873616 2311 66-67 0.1785567131902681 2311 68-69 0.24711180814329126 2311 70-71 0.4806847100060736 2311 72-73 0.5419103926777247 2311 74-75 0.7592362661519623 2311 76-77 0.7326698306429869 2311 78-79 0.5818907032598055 2311 80-81 0.5163533619120351 2311 82-83 0.6462762783815315 2311 84-85 0.5846558824589358 2311 86-87 0.7244495048845607 2311 88-89 1.1792891309984448 2311 90-91 1.5125607248523423 2311 92-93 1.141425931350124 2311 94-95 1.5749597011956382 2311 96-97 3.3465905761030648 2311 98-99 3.3001604882085918 2311 100-101 2.8801769802466453 2312 1 -0.07557600804802078 2312 2 -0.3508548968773795 2312 3 -0.6807584043653883 2312 4 -0.21097769784551446 2312 5 -2.1307970067007354 2312 6 -0.43807199733269186 2312 7 0.05532233687996069 2312 8 0.3147521525892145 2312 9 0.35674106355489243 2312 10-11 0.30007225575763385 2312 12-13 0.28605000597794117 2312 14-15 0.6412180572486861 2312 16-17 0.014069197982081505 2312 18-19 0.19561486272475292 2312 20-21 0.42839478687572097 2312 22-23 0.40144905616762117 2312 24-25 0.4516653385803906 2312 26-27 0.6861996208968222 2312 28-29 0.770227576918252 2312 30-31 0.44905813958072116 2312 32-33 -0.1570380271341918 2312 34-35 0.2702181341276102 2312 36-37 0.616766855794495 2312 38-39 0.7637060419553237 2312 40-41 0.6760549762476842 2312 42-43 0.7135639209733675 2312 44-45 0.8824870224154964 2312 46-47 0.4709227652708101 2312 48-49 0.6564099326366026 2312 50-51 0.633262779018132 2312 52-53 0.5325125341231391 2312 54-55 0.47390416094271615 2312 56-57 0.5925209744859501 2312 58-59 0.9697732599339162 2312 60-61 0.9093497618053981 2312 62-63 1.1555538115159507 2312 64-65 0.9536570864488993 2312 66-67 1.0985395632674368 2312 68-69 0.9149691463051361 2312 70-71 1.1903911258089153 2312 72-73 1.2007442738769143 2312 74-75 1.4165370596966653 2312 76-77 1.1754712682591943 2312 78-79 1.480294499635992 2312 80-81 1.5197089136868769 2312 82-83 1.7240448744271752 2312 84-85 1.5350813646011439 2312 86-87 1.4208259950594275 2312 88-89 1.5412656709977206 2312 90-91 1.730906281366579 2312 92-93 1.806707363254752 2312 94-95 2.3567409842346834 2312 96-97 3.691296050103233 2312 98-99 4.0771374383477355 2312 100-101 3.511136920214321 2313 1 -0.006327181756940803 2313 2 -0.43544917425334617 2313 3 -1.011389034996018 2313 4 -0.14774492281696894 2313 5 -0.16575608549783993 2313 6 0.11845160373316332 2313 7 0.09551235083758058 2313 8 0.1290825040674548 2313 9 0.21167698539222357 2313 10-11 -0.7208427613721753 2313 12-13 -0.1934806690633053 2313 14-15 -0.02445174988239529 2313 16-17 -0.5880569598659022 2313 18-19 0.030192486122807338 2313 20-21 -2.5165431968826866 2313 22-23 -0.4311015402033931 2313 24-25 0.0770880324009724 2313 26-27 0.08400764018370666 2313 28-29 -0.06382425660541458 2313 30-31 0.21960280396341147 2313 32-33 0.6009162299397843 2313 34-35 0.2516196060220395 2313 36-37 -0.8077937964069974 2313 38-39 -0.017480514319252904 2313 40-41 0.9176114094414345 2313 42-43 0.7958702590015356 2313 44-45 0.7913223542261818 2313 46-47 0.5948347053799345 2313 48-49 0.3867336542455355 2313 50-51 0.4562333728514041 2313 52-53 0.4547852469768401 2313 54-55 0.8500247357428705 2313 56-57 0.8628562745747743 2313 58-59 0.9280312538433186 2313 60-61 0.6141770362629551 2313 62-63 0.668192594665296 2313 64-65 0.43594994268541853 2313 66-67 0.8215228458457879 2313 68-69 0.7592195586893489 2313 70-71 0.7430073864357389 2313 72-73 0.527450117044026 2313 74-75 0.879799970494787 2313 76-77 0.9673584018716781 2313 78-79 0.7366008059422988 2313 80-81 0.9059257643403491 2313 82-83 1.127451802481989 2313 84-85 0.8143654021598294 2313 86-87 0.8455658757852262 2313 88-89 0.7082574867571374 2313 90-91 1.042505380465677 2313 92-93 0.9151715492717543 2313 94-95 1.3533213674347806 2313 96-97 2.495912374173784 2313 98-99 2.5817172822761716 2313 100-101 1.9662425540171373 2314 1 -0.3181551387461852 2314 2 -0.502701910860921 2314 3 -1.5562064330392218 2314 4 -0.5064778492918265 2314 5 -0.6917320614738216 2314 6 -1.2418070420093557 2314 7 -0.7012182507317348 2314 8 -2.363351865786271 2314 9 -1.929391502450457 2314 10-11 -1.149724869286544 2314 12-13 -1.1148867525661643 2314 14-15 -0.9497617372891582 2314 16-17 -1.0731654554582732 2314 18-19 -2.2168602117363463 2314 20-21 -2.034578167337017 2314 22-23 -2.4257712098730693 2314 24-25 -2.2164700418022605 2314 26-27 -2.0827437886644944 2314 28-29 -3.2935975783142197 2314 30-31 -1.6960999956103606 2314 32-33 -1.4126699368721916 2314 34-35 -2.0670401827990403 2314 36-37 -3.7805052175055174 2314 38-39 -3.9115883318141726 2314 40-41 -4.771265370564375 2314 42-43 -3.333610027736814 2314 44-45 -3.488441280376165 2314 46-47 -3.5500020668761927 2314 48-49 -1.7008692712606148 2314 50-51 -1.3067376627002787 2314 52-53 -2.6106342047329356 2314 54-55 -2.0578411947037054 2314 56-57 -2.163357035509506 2314 58-59 -3.8639623526664195 2314 60-61 -2.4345927012164594 2314 62-63 -2.2069502901550067 2314 64-65 -1.5732624955592804 2314 66-67 -2.6550367782944804 2314 68-69 -2.6603217568197053 2314 70-71 -2.13111835220613 2314 72-73 -1.6083655373200152 2314 74-75 -1.8021892768170424 2314 76-77 -0.8192249556794167 2314 78-79 -0.8875370415826467 2314 80-81 -0.9636446124880926 2314 82-83 -0.6500716887511793 2314 84-85 -0.24800018923802014 2314 86-87 -0.055661964958741095 2314 88-89 -0.2815831603415724 2314 90-91 0.15630950717303094 2314 92-93 0.23925853948777842 2314 94-95 0.8428205440307366 2314 96-97 2.3327863449187234 2314 98-99 2.411769108295738 2314 100-101 2.4796834142321913 2315 1 -2.4628832639054394 2315 2 -1.8133365402923687 2315 3 -1.3854977408582805 2315 4 -1.6322588206010025 2315 5 -1.499815263158915 2315 6 -2.6621683527161792 2315 7 -1.9202342014018683 2315 8 -1.2103137642387694 2315 9 -0.913317677517135 2315 10-11 -3.542361910853412 2315 12-13 -1.8985509051761937 2315 14-15 -1.1415880805258283 2315 16-17 -1.827478846207221 2315 18-19 -1.0596029965636689 2315 20-21 -1.0784726618927252 2315 22-23 -1.0609871129799657 2315 24-25 -2.8653140853565944 2315 26-27 -3.9071135758190216 2315 28-29 -5.987997007555414 2315 30-31 -8.432634504435036 2315 32-33 -6.849412534843957 2315 34-35 -5.7533033263890445 2315 36-37 -4.163081785818214 2315 38-39 -6.887569323507591 2315 40-41 -4.096408060265237 2315 42-43 -4.101286139102729 2315 44-45 -3.2572729235160196 2315 46-47 -2.398438425570923 2315 48-49 -3.101486319282497 2315 50-51 -2.853693615748572 2315 52-53 -2.1533591533476546 2315 54-55 -3.2358661314276205 2315 56-57 -3.139675641227285 2315 58-59 -2.120931215735265 2315 60-61 -2.020343266029858 2315 62-63 -2.6964566517375026 2315 64-65 -2.1141354507649055 2315 66-67 -2.4158816202316657 2315 68-69 -1.964248696295492 2315 70-71 -2.1417733658805567 2315 72-73 -1.5305491144149208 2315 74-75 -2.0056660642603497 2315 76-77 -1.3057874928381956 2315 78-79 -1.3322647420701692 2315 80-81 -1.155254373143105 2315 82-83 -1.1754878670453586 2315 84-85 -0.8995366515526513 2315 86-87 -1.4424491534620323 2315 88-89 -1.6739828104120669 2315 90-91 -1.3397690740836552 2315 92-93 -0.48332125633005774 2315 94-95 0.08918875140643223 2315 96-97 1.5865404430009047 2315 98-99 1.349950918756253 2315 100-101 0.941361037429461 2316 1 -3.36767983876177 2316 2 -2.0082262992043844 2316 3 -2.319888839147996 2316 4 -2.619895334097812 2316 5 -2.3407658778989386 2316 6 -5.719608195196205 2316 7 -3.3442056280108368 2316 8 -4.032792414329204 2316 9 -6.731409579433471 2316 10-11 -5.4644124179853115 2316 12-13 -3.4996498817542516 2316 14-15 -4.824863030728455 2316 16-17 -4.088526995118549 2316 18-19 -2.09866245577561 2316 20-21 -2.7522071216770527 2316 22-23 -3.7121238668778958 2316 24-25 -2.459979469883926 2316 26-27 -3.6916615072245698 2316 28-29 -3.420744220047112 2316 30-31 -3.3796203322162484 2316 32-33 -3.3335987197925583 2316 34-35 -5.039149423877426 2316 36-37 -5.138881405755477 2316 38-39 -5.370809930692154 2316 40-41 -4.688348898692784 2316 42-43 -3.9442263593559517 2316 44-45 -3.26282962383884 2316 46-47 -3.428138267593038 2316 48-49 -3.9327728087391876 2316 50-51 -4.8269106407273945 2316 52-53 -3.0055033024421434 2316 54-55 -3.193956201716329 2316 56-57 -2.4881817199414833 2316 58-59 -2.7926829386100067 2316 60-61 -2.8690714295942037 2316 62-63 -2.3579008901238403 2316 64-65 -2.301959706093797 2316 66-67 -3.0296783553554114 2316 68-69 -2.2532429037731134 2316 70-71 -1.7842350734154167 2316 72-73 -1.9514113531217951 2316 74-75 -3.1656106575245353 2316 76-77 -3.3919770205072197 2316 78-79 -2.931058157368838 2316 80-81 -3.107835823334284 2316 82-83 -2.1436083881434165 2316 84-85 -1.880320588178332 2316 86-87 -1.6115564639458064 2316 88-89 -1.734470023361677 2316 90-91 -1.3549028973773822 2316 92-93 -0.968416386490091 2316 94-95 -0.7696385490033943 2316 96-97 0.532248710510121 2316 98-99 0.5532409798868247 2316 100-101 0.44281260232631503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8514.0 3 2380.0 4 2332.0 5 2560.0 6 3111.0 7 4117.0 8 5294.0 9 6857.0 10 8468.0 11 11303.0 12 14453.0 13 17475.0 14 19569.0 15 22953.0 16 26287.0 17 27774.0 18 22210.0 19 18328.0 20 18708.0 21 19863.0 22 22161.0 23 24235.0 24 26547.0 25 29892.0 26 33200.0 27 37069.0 28 41085.0 29 46135.0 30 51940.0 31 58382.0 32 66855.0 33 75837.0 34 84768.0 35 89062.0 36 81428.0 37 59669.0 38 14427.0 39 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.72507596650681 19.205057490003217 13.919600038039862 18.150266505450116 2 16.01115047710131 22.86626672752697 39.83008020518617 21.292502590185553 3 19.703653115274392 30.832104517573576 29.75116933745394 19.71307302969809 4 11.651426511166955 18.08200514185393 37.316760305902264 32.94980804107686 5 11.425328504577232 41.27680620902185 31.333530203579535 15.964335082821378 6 27.066133295649514 37.545225115542586 18.5568364431545 16.831805145653405 7 26.0671995303697 38.073845378933854 19.897050949995787 15.961904140700659 8 27.927671659774155 31.920587395989507 19.50627573367879 20.645465210557546 9 30.312625421165972 16.141607800822808 20.026889392008016 33.518877386003204 10-11 25.001811889619685 28.652588284321716 24.877289775506874 21.46831005055172 12-13 28.03591124559206 27.186959978076388 26.591353307680286 18.185775468651265 14-15 24.225743028075208 26.783296820359336 28.55947145941534 20.43148869215011 16-17 19.535800536632117 31.043769396750857 28.314772239342993 21.10565782727403 18-19 19.145986790247616 27.39089270020748 34.72588404772907 18.737236461815844 20-21 21.415106651392776 25.456857300291464 34.901777039861955 18.22625900845381 22-23 20.342842329058115 26.41738694555974 34.85506270969894 18.384708015683206 24-25 19.756998873072416 26.872055187720022 35.06222580437516 18.3087201348324 26-27 19.24059068191348 26.878067024037534 35.627548534061674 18.253793759987307 28-29 18.577966886954883 27.85586640905427 35.2868990315962 18.279267672394646 30-31 18.78675370597035 27.437055170225307 35.09007661272043 18.68611451108391 32-33 18.41541930622914 27.808363004327568 34.91262810208091 18.863589587362377 34-35 19.121964395290046 27.734729411636742 34.7769516560151 18.3663545370581 36-37 19.215358765121497 27.464210491972057 34.57376136944488 18.746669373461565 38-39 18.796130708243183 27.840174176139428 34.58750547246275 18.776189643154638 40-41 18.7270225065582 28.105850200677096 34.415770677534994 18.75135661522971 42-43 18.576946135869587 28.205947381610436 33.53485423381953 19.682252248700447 44-45 18.741888060675493 27.950026828313295 33.51540634018301 19.792678770828196 46-47 19.147391180190105 28.61846548543403 32.70514058300131 19.529002751374556 48-49 18.974245983117783 28.826210368976035 32.056309115299854 20.143234532606325 50-51 18.725273389871163 29.17813679083799 31.99803025981617 20.098559559474683 52-53 19.156072101098278 29.551762797836094 30.922117244106772 20.370047856958852 54-55 18.23971730846238 29.09723825206148 31.43406003113707 21.228984408339073 56-57 19.003278248542106 29.175595976155062 30.729896529572017 21.09122924573082 58-59 19.44409563542451 28.501998486798474 30.890436140220924 21.163469737556092 60-61 19.2156523268283 28.814527233123364 31.469356787590975 20.500463652457356 62-63 19.060825934078085 28.79095421502637 31.343385716117844 20.8048341347777 64-65 18.66034323612403 29.11983266411971 31.223856600835866 20.995967498920393 66-67 18.966986768335538 29.018221462384165 30.542892007487417 21.471899761792884 68-69 19.05120113397633 28.832336089123 30.643372056981587 21.473090719919085 70-71 19.127055699311633 28.897977495502275 30.44270325875641 21.53226354642969 72-73 18.90692263892969 28.552666891521874 30.80144560136074 21.738964868187704 74-75 18.31933116008723 29.333200339135207 30.391955060051412 21.95551344072615 76-77 18.380733761899233 28.51970629208266 30.68011558219178 22.41944436382633 78-79 18.58045103781682 28.54899709673368 30.613929965217935 22.25662190023156 80-81 18.718904908648675 28.79008693475631 30.275214797590564 22.215793359004447 82-83 18.650497300949258 28.433008903126638 30.167293535654448 22.749200260269657 84-85 18.434056991356183 28.358581118881755 30.03289601938218 23.17446587037989 86-87 18.585172008616752 28.199412856945987 29.952772881896845 23.26264225254042 88-89 18.529027367955315 27.980675470023357 29.754327143421218 23.73597001860011 90-91 18.64758464552829 27.70442723562751 29.98119542343391 23.666792695410287 92-93 19.223909288334156 27.63164934010981 29.902251923451832 23.242189448104202 94-95 18.78095303730256 27.241347885048643 30.108602853063555 23.869096224585242 96-97 18.68145809112921 26.84037967695545 30.28455949326189 24.19360273865345 98-99 18.994837175962473 27.033100144268722 29.56256635000136 24.409496329767446 100-101 18.874243400455548 26.64900133397461 30.1121173897656 24.36463787580424 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6731.0 1 4565.5 2 2928.0 3 3474.5 4 2922.5 5 2687.5 6 4332.5 7 8222.5 8 12045.0 9 12485.0 10 9573.0 11 6810.0 12 5947.5 13 5614.5 14 5422.5 15 5299.0 16 5163.0 17 5066.0 18 5126.5 19 5253.5 20 5503.5 21 5999.0 22 6530.5 23 7079.0 24 7711.0 25 8545.5 26 9555.0 27 10835.0 28 12011.0 29 13132.0 30 14546.0 31 16192.0 32 18431.5 33 20422.0 34 22047.0 35 23508.5 36 25519.0 37 27730.0 38 28247.0 39 28081.5 40 28226.0 41 28988.5 42 30072.5 43 31049.5 44 31626.0 45 32253.5 46 33010.5 47 35893.5 48 42016.5 49 42207.0 50 36629.0 51 32405.5 52 29907.5 53 28876.5 54 27905.5 55 26151.5 56 24311.5 57 22487.0 58 20279.0 59 17919.0 60 15753.5 61 13894.5 62 11561.5 63 8560.5 64 5996.0 65 4509.5 66 3563.5 67 2847.5 68 2058.0 69 1378.0 70 1006.0 71 819.5 72 753.5 73 651.0 74 527.5 75 435.0 76 313.0 77 193.5 78 138.5 79 88.5 80 52.5 81 42.0 82 28.5 83 16.0 84 11.5 85 9.0 86 6.5 87 3.5 88 1.5 89 2.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11471240929532385 2 0.10901297571046153 3 0.1202309084806667 4 0.1348865948417412 5 0.12945856285615806 6 0.13126790685135245 7 0.13805294683333136 8 0.1373292092352536 9 0.14402378201747282 10-11 0.14049556122684378 12-13 0.1393647212298473 14-15 0.12914192765699906 16-17 0.13126790685135245 18-19 0.1486376092052185 20-21 0.17776804752784806 22-23 0.19491158188231483 24-25 0.2147691322295732 26-27 0.21807118502080294 28-29 0.21929249221755912 30-31 0.2192020250177994 32-33 0.22110183621275353 34-35 0.18925738189733238 36-37 0.1788536539249647 38-39 0.191971397890124 40-41 0.17944169072340285 42-43 0.18215570671619447 44-45 0.1864528987047811 46-47 0.20698895305023735 48-49 0.22585136420013877 50-51 0.24331153375376458 52-53 0.26425469049813954 54-55 0.25588647452036556 56-57 0.22594183139989849 58-59 0.21834258662008207 60-61 0.1968566266771488 62-63 0.18568392750682347 64-65 0.1776323467282085 66-67 0.17383272433830027 68-69 0.18084393231967852 70-71 0.18437215311030755 72-73 0.22083043461347435 74-75 0.2323197689829587 76-77 0.25158928253177887 78-79 0.23969284576337582 80-81 0.24489470974955965 82-83 0.2415926569583299 84-85 0.22652986819833668 86-87 0.2175736154221245 88-89 0.21915679141791955 90-91 0.22164463941131185 92-93 0.22960575299016714 94-95 0.23462668257683153 96-97 8.72194272883452 98-99 0.29519447281596345 100-101 0.3078598807823242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1105373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.200169871102496 #Duplication Level Percentage of deduplicated Percentage of total 1 77.51678996969723 35.812888644625865 2 10.459174602037773 9.66431286651332 3 4.217231230289458 5.845103976752746 4 2.26985846131918 4.194713859852219 5 1.3378461072785237 3.0904358708830504 6 0.8786367553351804 2.4355900410887794 7 0.6161051220046053 1.9924912906548367 8 0.43247491704285923 1.5984331705896848 9 0.31010452968277125 1.289419375422814 >10 1.760589351263878 14.71313785935075 >50 0.1181300513173693 3.7247211390104127 >100 0.07369734799531086 6.050750921778325 >500 0.005776278454070945 1.7054516875267132 >1k 0.0027885482192066633 2.8815903600718444 >5k 1.991820156576188E-4 0.8033558102901961 >10k+ 5.975460469728564E-4 4.197603125588465 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19936 1.8035540944097606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14615 1.3221781244882949 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11064 1.0009290981415322 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8730 0.7897786539023479 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4219 0.38168111578625497 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3741 0.3384377943011092 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3063 0.27710103286401966 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2972 0.2688685176858852 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2793 0.2526748889288955 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2528 0.22870108099256994 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2327 0.2105171738408664 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.16211722196941666 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1765 0.15967460757590424 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1618 0.14637592921122555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1237 0.11190792610277255 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.10105186213160626 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.046719975971911E-5 0.0 5 0.0 0.0 0.0 9.046719975971911E-5 0.0 6 0.0 0.0 0.0 9.046719975971911E-5 0.0 7 0.0 0.0 0.0 9.046719975971911E-5 0.0 8 0.0 0.0 0.0 9.046719975971911E-5 0.0 9 0.0 0.0 0.0 9.046719975971911E-5 0.0 10-11 0.0 0.0 0.0 9.046719975971911E-5 0.0 12-13 0.0 0.0 0.0 2.7140159927915733E-4 0.0 14-15 0.0 0.0 0.0 4.0710239891873605E-4 0.0 16-17 0.0 0.0 0.0 6.785039981978934E-4 0.0 18-19 0.0 0.0 0.0 9.499055974770507E-4 0.0 20-21 0.0 0.0 0.0 0.0021712127942332586 0.0 22-23 0.0 0.0 0.0 0.005699433584862305 0.0 24-25 0.0 0.0 0.0 0.012891575965759974 0.0 26-27 0.0 0.0 0.0 0.025104647933322056 0.0 28-29 0.0 0.0 0.0 0.05057116466568298 0.0 30-31 0.0 0.0 0.0 0.11805969568643346 0.0 32-33 0.0 0.0 0.0 0.21915679141791955 0.0 34-35 0.0 0.0 0.0 0.3571192710514912 0.0 36-37 0.0 0.0 0.0 0.5625250481059334 0.0 38-39 0.0 0.0 0.0 0.8972989208167741 0.0 40-41 0.0 0.0 0.0 1.2913288093702309 0.0 42-43 0.0 0.0 0.0 1.6315759476665344 0.0 44-45 0.0 0.0 0.0 2.006336322671171 0.0 46-47 0.0 0.0 0.0 2.448766163096077 0.0 48-49 0.0 0.0 0.0 2.9229047570367648 0.0 50-51 0.0 0.0 0.0 3.4490619908392914 0.0 52-53 0.0 0.0 0.0 3.940027483935287 0.0 54-55 0.0 0.0 0.0 4.415342151472851 0.0 56-57 0.0 0.0 0.0 4.956471706835611 0.0 58-59 0.0 0.0 0.0 5.528541044516195 0.0 60-61 0.0 0.0 0.0 6.182347497179685 0.0 62-63 0.0 0.0 0.0 6.794041468355026 0.0 64-65 0.0 0.0 0.0 7.341051391702168 0.0 66-67 0.0 0.0 0.0 7.937727807717394 0.0 68-69 0.0 0.0 0.0 8.601349951554814 0.0 70-71 0.0 0.0 0.0 9.329837077619953 0.0 72-73 0.0 0.0 0.0 10.028379560564623 0.0 74-75 0.0 0.0 0.0 10.684538160421866 0.0 76-77 0.0 0.0 0.0 11.315818280345187 0.0 78-79 0.0 0.0 0.0 12.039148776024021 0.0 80-81 0.0 0.0 0.0 12.820966316347514 0.0 82-83 0.0 0.0 0.0 13.57605079914201 0.0 84-85 0.0 0.0 0.0 14.287756259651719 0.0 86-87 0.0 0.0 0.0 14.884749310866106 0.0 88-89 0.0 0.0 0.0 15.478485542889143 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGTG 170 3.6379788E-12 33.194366 1 GTACATG 13210 0.0 32.928425 1 GTATCAA 17565 0.0 32.073086 1 TACATGG 13300 0.0 32.035263 2 ATGGGAG 2935 0.0 30.446445 5 ACATGGG 13670 0.0 30.273727 3 GGTATCA 11605 0.0 30.026527 1 GATGCGA 65 0.0061365124 28.939991 2 CATGGGA 9995 0.0 28.231886 4 GTGTAAG 285 0.0 28.05021 1 CATGGGG 3155 0.0 28.023985 4 CGTCACT 285 0.0 26.4002 1 TATCAAC 21530 0.0 26.276854 2 CATGGGT 860 0.0 26.249088 4 ATCAACG 22120 0.0 25.554718 3 TCAACGC 22270 0.0 25.257036 4 ATGGGAT 3565 0.0 25.197935 5 ATGGGCG 150 1.1556403E-6 25.083597 5 CAACGCA 22475 0.0 24.944088 5 AACGCAG 22970 0.0 24.5294 6 >>END_MODULE