##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939866_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105373 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.335799770756115 31.0 31.0 34.0 30.0 34.0 2 31.498541216403876 31.0 31.0 34.0 30.0 34.0 3 31.497990271157338 31.0 31.0 34.0 30.0 34.0 4 35.3554601026079 37.0 35.0 37.0 33.0 37.0 5 35.12976796067934 37.0 35.0 37.0 32.0 37.0 6 35.20263929008579 37.0 35.0 37.0 32.0 37.0 7 35.0964977432957 37.0 35.0 37.0 32.0 37.0 8 35.08041086583443 37.0 35.0 37.0 32.0 37.0 9 36.56338448650365 39.0 35.0 39.0 32.0 39.0 10-11 36.444889191250375 38.0 35.0 39.0 32.0 39.0 12-13 36.34485101409207 38.0 35.0 39.0 32.0 39.0 14-15 37.12395453842278 39.0 36.0 40.5 31.0 41.0 16-17 37.12748275921341 39.0 36.0 40.0 31.5 41.0 18-19 37.29311463189349 39.0 36.0 40.5 32.0 41.0 20-21 37.30891337132353 39.0 36.0 41.0 31.5 41.0 22-23 37.15323379528901 39.0 36.0 40.0 31.0 41.0 24-25 36.95164347238443 39.0 35.0 40.0 31.0 41.0 26-27 36.592019164571596 38.0 35.0 40.0 30.0 41.0 28-29 36.29805323632837 38.0 35.0 40.0 30.0 41.0 30-31 35.844670984364555 38.0 34.5 40.0 29.5 41.0 32-33 35.47280827376822 37.5 34.0 40.0 29.0 41.0 34-35 35.14469866732768 37.0 34.0 40.0 27.5 41.0 36-37 34.896873272641905 37.0 33.5 40.0 26.0 41.0 38-39 34.63938959970979 37.0 33.0 40.0 25.0 41.0 40-41 34.40574041522635 37.0 33.0 40.0 24.5 41.0 42-43 33.95265625268574 37.0 33.0 40.0 23.5 41.0 44-45 33.40689658603928 37.0 33.0 40.0 20.5 41.0 46-47 32.75574082232875 37.0 32.5 40.0 15.5 41.0 48-49 32.17224004928653 36.0 31.0 40.0 7.5 41.0 50-51 31.833436315162395 36.0 31.0 40.0 2.0 41.0 52-53 31.552722474675967 36.0 31.0 39.5 2.0 41.0 54-55 31.247383010078952 35.0 30.5 39.0 2.0 41.0 56-57 30.84347636499173 35.0 29.5 39.0 2.0 40.5 58-59 30.473827839109514 35.0 29.0 39.0 2.0 40.0 60-61 30.108944672974644 35.0 28.0 38.5 2.0 40.0 62-63 29.79715761105075 35.0 28.0 38.0 2.0 40.0 64-65 29.354602021218177 34.5 28.0 38.0 2.0 40.0 66-67 28.46387147143996 34.0 25.5 37.0 2.0 39.5 68-69 26.65807605215615 33.0 19.5 36.0 2.0 39.0 70-71 26.013582745371924 32.0 16.5 35.0 2.0 39.0 72-73 26.522061783669404 32.5 21.0 35.0 2.0 38.0 74-75 26.599808842806908 33.0 22.5 35.0 2.0 38.0 76-77 26.124726675972724 31.5 23.0 35.0 2.0 36.5 78-79 26.588447067189087 33.0 24.0 35.0 2.0 37.0 80-81 26.370130716056934 33.0 23.5 35.0 2.0 36.5 82-83 25.920562561234988 33.0 21.0 35.0 2.0 36.0 84-85 25.561533979932566 32.5 20.0 35.0 2.0 35.5 86-87 25.199424085806328 32.0 19.0 35.0 2.0 35.0 88-89 24.746997619807974 32.0 16.5 35.0 2.0 35.0 90-91 24.247090801023727 31.0 8.5 34.0 2.0 35.0 92-93 23.764899721632425 31.0 2.0 34.0 2.0 35.0 94-95 22.98617887355671 31.0 2.0 34.0 2.0 35.0 96-97 22.49160781021429 30.5 2.0 34.0 2.0 35.0 98-99 21.89381367194603 30.0 2.0 34.0 2.0 35.0 100-101 20.64906190037209 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19011696196492878 1101 2 0.31626543500055604 1101 3 0.34437760421151253 1101 4 0.38582438037614253 1101 5 0.42363935860002044 1101 6 0.23451368229462588 1101 7 0.13098493740996986 1101 8 0.3197024696601787 1101 9 0.4117108923854502 1101 10-11 0.3317226866118901 1101 12-13 0.4213819190024495 1101 14-15 0.1834874516077818 1101 16-17 0.33499591749671254 1101 18-19 0.36769968778450135 1101 20-21 0.5512778636193687 1101 22-23 0.36163853894107945 1101 24-25 0.33525457584951823 1101 26-27 0.6181476538513238 1101 28-29 0.5227961262813423 1101 30-31 0.22190264004331084 1101 32-33 0.2855081945721665 1101 34-35 0.2415336842907081 1101 36-37 0.5702245670601371 1101 38-39 0.5599074139556777 1101 40-41 0.5526993911366276 1101 42-43 0.36824476462359 1101 44-45 0.30061744355180053 1101 46-47 0.19720908560787365 1101 48-49 0.22646340319688107 1101 50-51 0.01676899385412156 1101 52-53 0.025769286550584525 1101 54-55 -0.2377240522594768 1101 56-57 -0.19474834007328923 1101 58-59 -0.028306302319396792 1101 60-61 -0.04791893049297258 1101 62-63 -0.2982149378824772 1101 64-65 -0.3736444573239446 1101 66-67 -0.26691943353639047 1101 68-69 -8.074366383175082 1101 70-71 -5.748857304771523 1101 72-73 -1.1105913372675147 1101 74-75 -0.042380082768897154 1101 76-77 -0.021231918354260415 1101 78-79 -0.43411084385312293 1101 80-81 -0.3439732213443847 1101 82-83 -0.11427042155809986 1101 84-85 -0.2585953991339025 1101 86-87 -0.4362398264412626 1101 88-89 -0.40284459576401943 1101 90-91 -0.60452054640702 1101 92-93 -0.5043022813236888 1101 94-95 -0.15362626538558644 1101 96-97 -0.9901140232422243 1101 98-99 -1.05546246477196 1101 100-101 -1.0153621320106758 1102 1 0.18630060033896 1102 2 0.06626990555302825 1102 3 0.2510384293562815 1102 4 0.14243260202017183 1102 5 0.12922516489396685 1102 6 0.11259575567724767 1102 7 0.22876486105293026 1102 8 0.10159986153986722 1102 9 0.3119803176810336 1102 10-11 0.35661248749348573 1102 12-13 0.182003951673245 1102 14-15 0.2301860976264578 1102 16-17 0.16525015781573416 1102 18-19 0.20400854633323462 1102 20-21 0.26385747670794757 1102 22-23 0.257390470964836 1102 24-25 0.27412200608655013 1102 26-27 0.2349065629173097 1102 28-29 0.28709816872775207 1102 30-31 0.17588687091455313 1102 32-33 0.31472325496932996 1102 34-35 0.14747847594806984 1102 36-37 0.4310186414918533 1102 38-39 0.6596283024630836 1102 40-41 0.7270375258433148 1102 42-43 0.6231675879861029 1102 44-45 0.6096007952144049 1102 46-47 0.5972028268344118 1102 48-49 0.41556553743863134 1102 50-51 0.3091218903250734 1102 52-53 0.22486347811078033 1102 54-55 -0.02204448357838018 1102 56-57 -0.0559078821694996 1102 58-59 -0.014025122451190697 1102 60-61 0.059377309176326776 1102 62-63 -0.013472784447156272 1102 64-65 -0.049947027295541346 1102 66-67 0.3999547412741329 1102 68-69 -3.6794705619495005 1102 70-71 0.40605665199525376 1102 72-73 0.46044015330408783 1102 74-75 0.10024320761732852 1102 76-77 -0.09604624140830254 1102 78-79 -0.1921727401422686 1102 80-81 0.04524797861150631 1102 82-83 -0.013097721135782336 1102 84-85 -0.05486561720745087 1102 86-87 -0.22889485775003138 1102 88-89 -0.31490510320152865 1102 90-91 -0.478426751235812 1102 92-93 -0.2859988708882284 1102 94-95 -0.06431654849077617 1102 96-97 -0.7946812141556769 1102 98-99 -0.7387446847887098 1102 100-101 -0.8729739181752123 1103 1 0.17549007291943752 1103 2 0.24423617227362016 1103 3 0.2482043545498911 1103 4 0.15324163151431947 1103 5 0.15280700404673553 1103 6 0.14574784004040708 1103 7 0.09915836604851336 1103 8 0.1363674584181922 1103 9 0.27980937443742704 1103 10-11 0.2862916318785125 1103 12-13 0.29024329018940165 1103 14-15 0.38762629295592177 1103 16-17 0.38454456006861193 1103 18-19 0.31785349607783786 1103 20-21 0.4643431790102639 1103 22-23 0.347341638597662 1103 24-25 0.16044669639047981 1103 26-27 0.2399380942464191 1103 28-29 0.16724616375464052 1103 30-31 0.3274151292767229 1103 32-33 0.44267454243618687 1103 34-35 0.4832017492145937 1103 36-37 0.16675577056808777 1103 38-39 0.2931825797889829 1103 40-41 0.4136880149159481 1103 42-43 0.3067113798343897 1103 44-45 0.38444575993825936 1103 46-47 0.36627516165069807 1103 48-49 0.24804130849572203 1103 50-51 0.23343733842944658 1103 52-53 0.34021384285560785 1103 54-55 0.35473542624651344 1103 56-57 0.5053377925709803 1103 58-59 0.2153394576054346 1103 60-61 0.31661902939382713 1103 62-63 0.39061130908330455 1103 64-65 0.1905400231396044 1103 66-67 0.18943368793301119 1103 68-69 -2.9894317656823297 1103 70-71 0.9294081851218756 1103 72-73 1.0877072633566662 1103 74-75 0.7147169115373977 1103 76-77 0.6225928184937324 1103 78-79 0.42763507603232753 1103 80-81 0.5187346995030708 1103 82-83 0.502393448594141 1103 84-85 0.34210932250105586 1103 86-87 0.3749540614968119 1103 88-89 0.3045057627491339 1103 90-91 0.14475851217436642 1103 92-93 0.29494489699325754 1103 94-95 0.23043472100097873 1103 96-97 0.03361272374033675 1103 98-99 0.2791270628408249 1103 100-101 0.2874265521733541 1104 1 0.08397179738054206 1104 2 0.05818716972977356 1104 3 0.09913709878160404 1104 4 0.05233343318344197 1104 5 0.07175420804549049 1104 6 0.17417199320682641 1104 7 0.07976487294367729 1104 8 0.19588457360541156 1104 9 0.19450567470073565 1104 10-11 0.16740690835461436 1104 12-13 0.08051641912232554 1104 14-15 0.13203540617947596 1104 16-17 -0.01411434116760546 1104 18-19 0.0017513851303689876 1104 20-21 0.12254485705729934 1104 22-23 0.09446195814065561 1104 24-25 0.04161329611378051 1104 26-27 -0.02067019780518109 1104 28-29 0.02157628380640375 1104 30-31 0.016848118967445203 1104 32-33 -0.12580071083287692 1104 34-35 -0.023984621116106553 1104 36-37 -0.3281833109689387 1104 38-39 -0.1986547924607578 1104 40-41 -0.4719749475511392 1104 42-43 -0.49061646414293847 1104 44-45 -0.48667710345264936 1104 46-47 -0.5778215491985961 1104 48-49 -0.4633658410557615 1104 50-51 -0.45949902074402615 1104 52-53 -0.5971617540560707 1104 54-55 -0.6001771010886934 1104 56-57 -0.4359852314840005 1104 58-59 -0.4984140569212805 1104 60-61 -0.48565146643337087 1104 62-63 -0.3371300217508093 1104 64-65 -0.2824215513683619 1104 66-67 -0.3799296806571597 1104 68-69 -3.7589022436579604 1104 70-71 0.18074035810353095 1104 72-73 0.24141257865992216 1104 74-75 -0.03140372040797601 1104 76-77 0.013296616414024243 1104 78-79 -0.3116093565110738 1104 80-81 -0.6094060600809854 1104 82-83 -0.7468936045588919 1104 84-85 -0.7872955509759869 1104 86-87 -0.5622831857919799 1104 88-89 -0.6365171318820124 1104 90-91 -0.7213235157633342 1104 92-93 -0.5930610832807659 1104 94-95 -0.5406926052809844 1104 96-97 -1.0691107555295325 1104 98-99 -0.6688434374492651 1104 100-101 -0.815929541904385 1105 1 0.16091143798152174 1105 2 0.1850350939108658 1105 3 0.1557036416898967 1105 4 0.09512928477921889 1105 5 0.22496398877857615 1105 6 0.17095456943875575 1105 7 0.24847531656306643 1105 8 0.12038902567329046 1105 9 0.22733250998451382 1105 10-11 0.0626583789039401 1105 12-13 0.18524290135668053 1105 14-15 0.20669205753067388 1105 16-17 0.2882566164752802 1105 18-19 -0.029851094740521944 1105 20-21 0.2101424894802335 1105 22-23 0.12555404495378042 1105 24-25 0.19528092403908204 1105 26-27 0.024428852988670258 1105 28-29 0.239688292484189 1105 30-31 0.3232805413097992 1105 32-33 0.11610171406695002 1105 34-35 0.23525867432540792 1105 36-37 0.1370982025871612 1105 38-39 0.22950214844388483 1105 40-41 7.269241105447577E-4 1105 42-43 -0.2031027581234568 1105 44-45 -0.24009736161085016 1105 46-47 -0.04062532309409761 1105 48-49 -0.001991094231293289 1105 50-51 0.0027947275473110267 1105 52-53 -0.14039377308135315 1105 54-55 0.0028910630249043834 1105 56-57 0.0034558421860388933 1105 58-59 -0.35098762848213383 1105 60-61 -0.012037942376920796 1105 62-63 -0.05694811751803641 1105 64-65 -0.192302084852205 1105 66-67 0.4226011193040371 1105 68-69 1.6606213747899758 1105 70-71 1.920921262862496 1105 72-73 0.9750546465124366 1105 74-75 0.38397061192882376 1105 76-77 -0.07967796461537446 1105 78-79 0.07778931301291436 1105 80-81 0.05046344979469808 1105 82-83 0.006064029510230995 1105 84-85 -0.17897059558485395 1105 86-87 -0.19107751172039045 1105 88-89 0.07948826527654518 1105 90-91 -0.08631959135882639 1105 92-93 -0.16869405886126998 1105 94-95 0.15664982326398658 1105 96-97 -0.36767820090387815 1105 98-99 -0.08762773924332024 1105 100-101 -0.002925211831101393 1106 1 0.32471367973273857 1106 2 0.22636339800710203 1106 3 0.23184653267228938 1106 4 0.20998145715939387 1106 5 0.2592662608279852 1106 6 0.38495579553170955 1106 7 0.14597772271147846 1106 8 0.20537505309275872 1106 9 0.21366999403861087 1106 10-11 0.15815748451713318 1106 12-13 0.23916502163183395 1106 14-15 0.4635774194130349 1106 16-17 0.29603892280492516 1106 18-19 0.5300189142230209 1106 20-21 0.4894398547969061 1106 22-23 0.4672877152134731 1106 24-25 0.568644723447747 1106 26-27 0.5476829099949114 1106 28-29 0.45535434505509187 1106 30-31 0.616130247338674 1106 32-33 0.5861840228361928 1106 34-35 0.5940895734418845 1106 36-37 0.7139721849418095 1106 38-39 0.8027820572817461 1106 40-41 0.7772773847610708 1106 42-43 0.643427469632357 1106 44-45 0.9359756467947307 1106 46-47 0.9865493983002764 1106 48-49 0.86511160403888 1106 50-51 0.8830130004246115 1106 52-53 0.9395344241449131 1106 54-55 1.0639489970976221 1106 56-57 1.0627434963428293 1106 58-59 1.150841673880418 1106 60-61 1.1945004855641486 1106 62-63 1.2919382863072464 1106 64-65 1.1313919648195103 1106 66-67 1.6796462352752748 1106 68-69 2.8887372287700117 1106 70-71 3.0794701133806726 1106 72-73 2.084223350765118 1106 74-75 1.6503644899632661 1106 76-77 1.2879830859999153 1106 78-79 1.0581344448021675 1106 80-81 1.0415904086513308 1106 82-83 1.0798818295783796 1106 84-85 0.9413789274744282 1106 86-87 0.6871489503246337 1106 88-89 1.225986306124799 1106 90-91 1.2122352243612902 1106 92-93 0.9286410383250931 1106 94-95 1.4708282740900245 1106 96-97 1.2562712979168502 1106 98-99 1.723342644866893 1106 100-101 1.8042252395332206 1107 1 0.046380322566161425 1107 2 0.06704531477188169 1107 3 0.13493023661256132 1107 4 0.047468130404034525 1107 5 0.08995569300889628 1107 6 0.22176560465921114 1107 7 -0.0066487801731440754 1107 8 -0.06792790079944666 1107 9 -0.11002755892282323 1107 10-11 0.03186393522283737 1107 12-13 0.18456623839836794 1107 14-15 0.21361714148563493 1107 16-17 0.23513783553235612 1107 18-19 0.22906741169590106 1107 20-21 -0.04712868246159729 1107 22-23 0.04772967888207802 1107 24-25 0.1986383838049619 1107 26-27 0.32806473444559714 1107 28-29 0.11296874859639416 1107 30-31 0.15427177867223207 1107 32-33 -0.06270979688597578 1107 34-35 0.06801322444056979 1107 36-37 0.20990226138296464 1107 38-39 0.23733954544854186 1107 40-41 0.259712284256274 1107 42-43 0.375338434989942 1107 44-45 0.3938047084163827 1107 46-47 0.3087251986603192 1107 48-49 0.1340292411157762 1107 50-51 0.02486568555727331 1107 52-53 0.11220428764397994 1107 54-55 -0.02494741250368193 1107 56-57 -0.12626842504559832 1107 58-59 -0.10282665952441405 1107 60-61 0.16971550114314482 1107 62-63 0.29401058683491144 1107 64-65 0.14908139541154597 1107 66-67 0.6290597785953729 1107 68-69 1.7432499739942315 1107 70-71 2.0667284641519963 1107 72-73 1.4012976453408292 1107 74-75 0.7976229519563951 1107 76-77 0.3205718688043895 1107 78-79 0.32574085626245974 1107 80-81 0.4258573695791128 1107 82-83 0.57560296791765 1107 84-85 0.5552275514223908 1107 86-87 0.36273640005635244 1107 88-89 0.32167598458256563 1107 90-91 0.25425204109794564 1107 92-93 0.006332645686704552 1107 94-95 0.39261312981151164 1107 96-97 0.15794486827973842 1107 98-99 0.4117011381610105 1107 100-101 0.7047246254299786 1108 1 -0.15826939677753415 1108 2 -0.06462926784181988 1108 3 -0.1240426692596337 1108 4 0.08595492516511172 1108 5 0.05886761010734176 1108 6 0.15874387178644156 1108 7 0.16037770226785142 1108 8 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-0.18814442119066754 2316 74-75 -0.8637447234459721 2316 76-77 -0.8212618775348766 2316 78-79 -0.7716496971654863 2316 80-81 -0.8874702067018347 2316 82-83 -0.8450122857681173 2316 84-85 -1.0343509322200717 2316 86-87 -1.0666671192548058 2316 88-89 -0.8639238752457246 2316 90-91 -0.9250388402316823 2316 92-93 -0.5982729962246367 2316 94-95 -0.22473812547621108 2316 96-97 -0.429251947819985 2316 98-99 -0.2429320374791466 2316 100-101 -0.7181258664225432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 45.0 5 146.0 6 349.0 7 831.0 8 1478.0 9 2668.0 10 4503.0 11 6703.0 12 9072.0 13 11388.0 14 13897.0 15 17567.0 16 21514.0 17 24779.0 18 20184.0 19 13759.0 20 12378.0 21 12477.0 22 13180.0 23 14484.0 24 16192.0 25 18602.0 26 21953.0 27 26239.0 28 31288.0 29 36316.0 30 42664.0 31 50237.0 32 59308.0 33 71316.0 34 89729.0 35 114388.0 36 124292.0 37 125748.0 38 70020.0 39 5667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.40813789246899 20.147201604124685 14.928095798673665 18.51656470473266 2 15.917041750596786 23.834706077174083 38.701603314131205 21.54664885809792 3 19.48898697543725 30.494231359007323 30.647935131399084 19.368846534156344 4 11.785523981497649 18.80442167485546 36.80983704143308 32.60021730221382 5 11.182198226300082 41.95000239738079 31.52899518985899 15.338804186460136 6 27.228299613161905 38.11712256145442 17.884386432802714 16.770191392580962 7 25.484791106712397 38.081534468455445 19.899979463945655 16.533694960886507 8 27.908769257074304 32.6912273051721 19.221656400147282 20.178347037606308 9 29.260620623083796 16.350679815772594 20.109953834587962 34.278745726555655 10-11 24.851023138795682 28.971985022250408 24.886033945102696 21.290957893851214 12-13 27.530797296478205 27.369720447306022 26.827912387945062 18.27156986827071 14-15 23.45303350090874 27.182046241404485 28.71478677333353 20.650133484353244 16-17 19.17751522114767 31.101893483629915 28.677049313804424 21.043541981417988 18-19 18.90637820898466 27.499676579760855 34.738273867735145 18.855671343519337 20-21 20.92216835403072 25.72461965327541 34.943770111989345 18.40944188070452 22-23 19.99822684288471 26.60391560134 34.94883627517589 18.4490212805994 24-25 19.458327396420664 27.107739698608384 35.09088565166946 18.34304725330149 26-27 18.947318425772426 27.207376732172783 35.65420550179012 18.191099340264664 28-29 18.406390973532428 27.965665841755467 35.35259412785656 18.275349056855546 30-31 18.650358364978164 27.63470067647185 35.07738376603257 18.63755719251742 32-33 18.2727977979676 27.942334615741277 34.86647803735041 18.918389548940713 34-35 18.93487376901607 27.913943084098197 34.83160908881179 18.319574058073943 36-37 19.07109451979366 27.572242870805074 34.66045760157654 18.696205007824727 38-39 18.604993382575007 27.938709468554894 34.59760939579265 18.85868775307745 40-41 18.491226481309575 28.140285733692576 34.522245115131206 18.84624266986664 42-43 18.38698780253658 28.291811723620768 33.592383015566526 19.728817458276126 44-45 18.49302942227225 28.11359942983848 33.50871656153483 19.88465458635443 46-47 18.917886119965228 28.758602578962623 32.63813930744712 19.685371993625036 48-49 18.828402816954977 28.875282641787248 32.00229463929158 20.2940199019662 50-51 18.551146435186716 29.17882541612919 31.989075575843657 20.280952572840434 52-53 19.058657902837382 29.520252774910823 30.899786336393433 20.521302985858366 54-55 18.14064160569801 28.99962522608644 31.422076899261857 21.437656268953692 56-57 18.928947160035747 29.0989264584441 30.647722259301574 21.324404122218578 58-59 19.16606460468165 28.53276174767322 30.846353630626037 21.454820017019088 60-61 18.983689542624106 28.99244304006478 31.359551603790013 20.6643158135211 62-63 18.90200488351664 29.04864608617534 31.1402893741445 20.909059656163517 64-65 18.39826585254861 29.299881392565275 31.06501514936304 21.23683760552307 66-67 18.658311982221377 29.134923169977505 30.58679878304325 21.619966064757868 68-69 18.83517923359929 28.944106885030806 30.613958331629636 21.606755549740267 70-71 19.004385337393913 29.05414336033575 30.2203569587424 21.721114343527937 72-73 18.731089892915996 28.86092494330367 30.52986369313825 21.878121470642085 74-75 18.398585416816545 29.583810117183628 29.898001059670488 22.119603406329336 76-77 18.24073076947441 29.050602383471176 30.123381600630573 22.58528524642384 78-79 18.304691422807075 29.0491850739382 30.153440421961157 22.492683081293567 80-81 18.395998749722374 29.348955470846683 29.756924136102942 22.498121643328002 82-83 18.396513392544144 29.141443269124213 29.49137055607951 22.970672782252137 84-85 18.098447979424 28.83770119476318 29.464236037763076 23.599614788049745 86-87 18.230017401641373 28.956611696418026 29.345400642598698 23.467970259341904 88-89 18.13354550993628 28.70970178723204 29.174433684332417 23.98231901849926 90-91 18.273540166786386 28.441077434985335 29.332908737920288 23.952473660307987 92-93 18.594189341275328 28.5719520006369 29.231879914741782 23.601978743345985 94-95 18.139324528291638 27.91474978790214 29.791442383174143 24.154483300632084 96-97 18.016429694696136 27.709479059784982 30.052732823377614 24.22135842214127 98-99 18.28847818228623 27.92940623986297 29.256262732786904 24.5258528450639 100-101 17.866817339567742 27.61177420055299 29.894396270936173 24.627012188943095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7047.0 1 4645.5 2 2789.0 3 3483.5 4 3030.0 5 2890.5 6 4924.0 7 9340.5 8 12742.0 9 12733.5 10 9941.5 11 6958.5 12 6094.0 13 5751.0 14 5458.5 15 5201.5 16 4903.5 17 4839.5 18 4890.0 19 5021.5 20 5275.0 21 5610.0 22 6077.5 23 6634.5 24 7284.0 25 8137.0 26 9153.5 27 10480.5 28 11615.5 29 12776.5 30 14394.0 31 15985.5 32 18205.0 33 20322.5 34 21774.5 35 23216.5 36 25407.0 37 27598.5 38 28126.0 39 27942.5 40 27936.5 41 28757.5 42 29900.5 43 31120.5 44 31951.0 45 32549.0 46 33213.5 47 36464.0 48 42935.0 49 42754.5 50 37165.0 51 32892.0 52 29783.0 53 28573.0 54 28103.5 55 26907.0 56 25068.5 57 22966.0 58 20780.0 59 18386.0 60 16096.5 61 14012.5 62 11391.0 63 8097.5 64 5474.0 65 4202.5 66 3311.0 67 2614.5 68 1801.0 69 1059.5 70 772.5 71 694.0 72 614.5 73 555.0 74 461.0 75 334.0 76 229.5 77 129.0 78 63.0 79 29.5 80 18.0 81 14.5 82 8.0 83 4.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020083718346657644 2 0.025964086331039388 3 0.0 4 0.0 5 0.0 6 9.046719975971913E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.714015992791574E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.5233599879859564E-5 26-27 4.975695986784551E-4 28-29 1.357007996395787E-4 30-31 4.975695986784551E-4 32-33 0.002759249592671433 34-35 0.005247097586063709 36-37 0.10684176291622827 38-39 0.09874494853773341 40-41 0.1083797053121435 42-43 0.10937484450950041 44-45 0.10173036612980416 46-47 0.09526196134698424 48-49 0.09589523174530226 50-51 0.09575953094566268 52-53 0.07535917739984602 54-55 0.06396031023012141 56-57 0.03487510550737172 58-59 0.012982043165519694 60-61 0.00909195357585177 62-63 0.0019902783947138205 64-65 0.004161491188947079 66-67 0.16288619316737427 68-69 0.4365947060404045 70-71 0.41121865650780326 72-73 0.19242373388892256 74-75 0.028270999924912228 76-77 0.03130165111686282 78-79 0.005247097586063709 80-81 0.0016736431955548037 82-83 4.5233599879859564E-5 84-85 6.785039981978934E-4 86-87 0.0015831759957950844 88-89 4.0710239891873605E-4 90-91 6.332703983180338E-4 92-93 0.0022616799939929777 94-95 0.08413449577653878 96-97 0.016193628756989722 98-99 0.01850054235086256 100-101 0.009860924773809383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1105373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.21979481675289 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06140161096182 28.262333853895495 2 12.624740305554718 9.902794487972926 3 5.181426642945658 6.096434693831563 4 2.826413666362365 4.434054562479928 5 1.7217468837932177 3.376327975437684 6 1.091501537114008 2.5685079796669066 7 0.7997939268237367 2.195742759399842 8 0.6046874633528719 1.8972574592769935 9 0.438153361917633 1.5465856457412033 >10 2.3644440801118485 16.91693158240406 >50 0.17049669880141385 4.61360016756813 >100 0.10229211424290967 7.154421345338657 >500 0.008063567511175682 2.036749781079919 >1k 0.003916589933999617 3.4921390150914653 >5k 0.0 0.0 >10k+ 9.215505727057923E-4 5.5061186908152715 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20824 1.8838889677963908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16736 1.514059055178659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13283 1.201675814408349 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10094 0.9131759143746048 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4767 0.43125714125458103 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4673 0.42275322447716746 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3301 0.2986322264068328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3297 0.29827035760779397 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3007 0.27203486967747537 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2748 0.24860386493970815 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2655 0.24019041536205427 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 0.19142859469156565 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2097 0.189709717896131 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1810 0.16374563156509161 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1236 0.11181745890301283 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1235 0.1117269917032531 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1219 0.11027951650709761 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.1083797053121435 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1134 0.10258980452752146 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.046719975971911E-5 0.0 5 0.0 0.0 0.0 9.046719975971911E-5 0.0 6 0.0 0.0 0.0 9.046719975971911E-5 0.0 7 0.0 0.0 0.0 9.046719975971911E-5 0.0 8 0.0 0.0 0.0 9.046719975971911E-5 0.0 9 0.0 0.0 0.0 9.046719975971911E-5 0.0 10-11 0.0 0.0 0.0 9.046719975971911E-5 0.0 12-13 0.0 0.0 0.0 2.7140159927915733E-4 0.0 14-15 0.0 0.0 0.0 4.0710239891873605E-4 0.0 16-17 0.0 0.0 0.0 6.785039981978934E-4 0.0 18-19 0.0 0.0 0.0 8.594383977173316E-4 0.0 20-21 0.0 0.0 0.0 0.0021712127942332586 0.0 22-23 0.0 0.0 0.0 0.005880367984381743 0.0 24-25 0.0 0.0 0.0 0.013660547163717587 0.0 26-27 0.0 0.0 0.0 0.026190254330438686 0.0 28-29 0.0 0.0 0.0 0.05269714386003639 0.0 30-31 0.0 0.0 0.0 0.1223568876750201 0.0 32-33 0.0 0.0 0.0 0.22802257699437203 0.0 34-35 0.0 0.0 0.0 0.3729962646093219 0.0 36-37 0.0 0.0 0.0 0.5883986672372131 0.0 38-39 0.0 0.0 0.0 0.9373758903103298 0.0 40-41 0.0 0.0 0.0 1.3463781004240198 0.0 42-43 0.0 0.0 0.0 1.6995620482859632 0.0 44-45 0.0 0.0 0.0 2.09019941684843 0.0 46-47 0.0 0.0 0.0 2.550179894026722 0.0 48-49 0.0 0.0 0.0 3.0389289407286046 0.0 50-51 0.0 0.0 0.0 3.580827467289322 0.0 52-53 0.0 0.0 0.0 4.088077056342067 0.0 54-55 0.0 0.0 0.0 4.5802186230349395 0.0 56-57 0.0 0.0 0.0 5.1300782631745125 0.0 58-59 0.0 0.0 0.0 5.684235095302672 0.0 60-61 0.0 0.0 0.0 6.294164956082698 0.0 62-63 0.0 0.0 0.0 6.857866077785507 0.0 64-65 0.0 0.0 0.0 7.37551939481062 0.0 66-67 0.0 0.0 0.0 7.910225778990441 0.0 68-69 0.0 0.0 0.0 8.514275271786085 0.0 70-71 0.0 0.0 0.0 9.229599420286185 0.0 72-73 0.0 0.0 0.0 9.934067504815117 0.0 74-75 0.0 0.0 0.0 10.616868695001596 0.0 76-77 0.0 0.0 0.0 11.280174203639858 0.0 78-79 0.0 0.0 0.0 12.046386152004798 0.0 80-81 0.0 0.0 0.0 12.870994677814638 0.0 82-83 0.0 0.0 0.0 13.673529206883106 0.0 84-85 0.0 0.0 0.0 14.425944907284691 0.0 86-87 0.0 0.0 0.0 15.145656714973136 0.0 88-89 0.0 0.0 0.0 15.902233906563666 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 70 2.0650572E-4 33.895554 2 GGTAAGG 380 0.0 32.456623 3 GGTATCA 13275 0.0 31.824665 1 GTCGGGA 675 0.0 31.63585 2 GTAAGGT 415 0.0 30.862368 4 GTATCAA 19300 0.0 30.37512 1 CGGGAGT 730 0.0 29.241743 4 TAAGGTG 455 0.0 29.191755 5 AGGTAAG 380 0.0 28.722021 2 GTACATG 14250 0.0 27.914993 1 TACATGG 14400 0.0 27.285921 2 TATCACG 70 0.008431377 27.116444 2 TCGGGAG 790 0.0 27.020851 3 ACATGGG 14340 0.0 26.033895 3 GGTCGGG 895 0.0 25.458134 1 AGGTGTG 485 0.0 25.429932 7 ATGGGAG 3005 0.0 25.257425 5 AAGGCGT 95 0.0012361113 24.966633 6 TATCAAC 23835 0.0 24.548147 2 CATGGGC 1055 0.0 24.280346 4 >>END_MODULE