FastQCFastQC Report
Fri 27 May 2016
SRR939865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732804
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78030.1648705503122462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.1174990555281816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.048.0735171
GTATCAA106750.042.3080141
TCAACGC135800.033.958794
TATCAAC135750.033.9345552
ATCAACG137350.033.64433
CAACGCA138800.033.2251635
AACGCAG148450.031.4165926
ACGCAGA172350.026.7021477
TACATGG171000.026.5786022
CGCAGAG177050.025.939448
GTACATG173600.025.852051
ACATGGG175050.024.7180293
GCAGAGT193450.022.3176889
CATGGGG115950.020.4590284
AGAGTAC166800.019.90927910-11
GAGTACA128350.019.589961
GAGTACT93750.019.02739312-13
CAGAGTA177850.018.91236910-11
AGTACTT97500.018.75782612-13
GGACCGT6250.018.2186836
Produced by FastQC (version 0.11.5)