##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939865_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4732804 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.8990562465718 31.0 30.0 33.0 16.0 34.0 2 29.745872003150776 31.0 30.0 33.0 25.0 34.0 3 30.030455095964253 31.0 30.0 34.0 25.0 34.0 4 33.80187347711843 35.0 35.0 37.0 28.0 37.0 5 33.738220936256816 35.0 35.0 37.0 28.0 37.0 6 33.760550193923095 35.0 35.0 37.0 28.0 37.0 7 33.66893030009271 35.0 33.0 37.0 28.0 37.0 8 33.5297916414878 35.0 33.0 37.0 28.0 37.0 9 34.81784033313021 37.0 34.0 39.0 28.0 39.0 10-11 34.63954845795431 37.0 34.0 39.0 27.0 39.0 12-13 34.51173025546801 37.0 33.5 39.0 27.0 39.0 14-15 35.196949525059566 38.0 33.5 40.0 26.5 41.0 16-17 35.00656175493428 38.0 33.0 40.0 26.5 41.0 18-19 34.789521602838406 38.0 33.0 40.0 25.0 41.0 20-21 34.95159867173878 38.0 34.0 40.0 25.0 41.0 22-23 34.840567769127986 38.0 33.5 40.0 25.0 41.0 24-25 34.61703685595262 38.0 33.0 40.0 24.5 41.0 26-27 34.50678371637617 38.0 33.0 40.0 24.0 41.0 28-29 34.572477330563444 38.0 33.0 40.0 24.0 41.0 30-31 34.36735844121159 38.0 33.0 40.0 24.0 41.0 32-33 34.04046089802155 38.0 33.0 40.0 22.5 41.0 34-35 33.868583824726315 38.0 32.5 40.0 22.5 41.0 36-37 33.66934971319328 38.0 32.0 40.0 20.5 41.0 38-39 33.440141721482654 37.5 31.5 40.0 19.5 41.0 40-41 33.202024106639534 37.0 31.0 40.0 18.0 41.0 42-43 32.80803050369295 37.0 31.0 40.0 15.5 41.0 44-45 32.47836092515135 37.0 30.5 40.0 14.5 41.0 46-47 32.119365707939735 36.5 30.0 39.0 11.0 41.0 48-49 31.76418081120621 36.0 30.0 39.0 9.0 40.5 50-51 31.113483888198203 35.5 29.0 38.5 8.5 40.0 52-53 31.25408996442701 35.5 29.5 39.0 8.0 40.0 54-55 31.334135218783622 36.0 29.5 39.0 7.5 40.0 56-57 30.9993668658157 35.5 29.0 39.0 6.0 40.0 58-59 30.569698956474852 35.0 28.0 39.0 2.0 40.0 60-61 30.126836226473777 35.0 28.0 38.0 2.0 40.0 62-63 29.650239900067696 34.0 27.5 38.0 2.0 40.0 64-65 29.08269357869035 34.0 26.0 37.5 2.0 40.0 66-67 28.46828539698665 34.0 26.0 37.0 2.0 39.0 68-69 27.915296513441078 33.0 25.5 36.0 2.0 39.0 70-71 27.32948311402712 32.5 24.5 36.0 2.0 39.0 72-73 26.671583378479227 32.0 22.5 35.0 2.0 38.0 74-75 26.306405145871246 32.0 22.0 35.0 2.0 37.5 76-77 25.73625265276145 31.5 20.5 35.0 2.0 37.0 78-79 25.077476692463918 31.0 18.5 34.5 2.0 36.0 80-81 24.352886893266657 31.0 15.0 34.0 2.0 36.0 82-83 23.609583980236664 30.0 7.5 34.0 2.0 35.0 84-85 22.8389694565843 29.5 3.5 34.0 2.0 35.0 86-87 21.951360969099923 29.0 2.0 34.0 2.0 35.0 88-89 20.97715994577422 28.0 2.0 33.5 2.0 35.0 90-91 19.570558806153812 26.0 2.0 32.5 2.0 34.5 92-93 18.02122188030605 22.0 2.0 32.0 2.0 34.0 94-95 17.460196112072254 20.0 2.0 32.0 2.0 34.0 96-97 17.073280976774022 19.5 2.0 32.0 2.0 34.0 98-99 16.124483921159634 10.0 2.0 31.5 2.0 34.0 100-101 14.492972136602319 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6357067941801517 1101 2 0.9849785086260532 1101 3 0.9953041103516078 1101 4 0.8755366437707082 1101 5 1.0289242762024315 1101 6 1.0374048225282522 1101 7 0.9921500729121178 1101 8 1.082260352063102 1101 9 1.0701963503324095 1101 10-11 1.196108873451145 1101 12-13 1.1675699323746542 1101 14-15 1.3428470184513728 1101 16-17 1.0903028365570435 1101 18-19 1.1237062430950644 1101 20-21 1.1313993028415013 1101 22-23 1.275838369581784 1101 24-25 1.4846888132183622 1101 26-27 1.3805882460701753 1101 28-29 1.2731689793585304 1101 30-31 1.44638309918178 1101 32-33 1.4426181481324463 1101 34-35 1.3314421020159344 1101 36-37 1.2503490645684394 1101 38-39 1.223593184581489 1101 40-41 1.1197361336041283 1101 42-43 1.2440041320953767 1101 44-45 1.2654217042511888 1101 46-47 1.0936688885904218 1101 48-49 1.1108778884056925 1101 50-51 1.0276330941787073 1101 52-53 0.991050012367662 1101 54-55 1.1221725764219954 1101 56-57 1.0473652567580807 1101 58-59 0.8745500280728216 1101 60-61 0.7846679690292007 1101 62-63 0.47119155918152344 1101 64-65 0.6501254297319043 1101 66-67 0.8588587944756227 1101 68-69 0.5923555579786388 1101 70-71 0.6393604801883832 1101 72-73 0.529325917900632 1101 74-75 0.41103118315506393 1101 76-77 0.1930827399176387 1101 78-79 0.26435420988774894 1101 80-81 0.0809144138659299 1101 82-83 0.047901793180411545 1101 84-85 -0.057700444068689904 1101 86-87 -0.2861892940638917 1101 88-89 -4.619449479651422 1101 90-91 -8.052472588971305 1101 92-93 -10.345160486372073 1101 94-95 -8.4110262340776 1101 96-97 -5.03436426699292 1101 98-99 -3.4190592918015827 1101 100-101 -2.4628519627899284 1102 1 1.9852552229822749 1102 2 1.3575759127474143 1102 3 1.1286796533742596 1102 4 1.132667337462614 1102 5 1.1478179785374891 1102 6 1.2411324074748222 1102 7 1.1820971261546518 1102 8 1.3063476408722892 1102 9 1.6629498129592335 1102 10-11 1.484261833703286 1102 12-13 1.512169508068915 1102 14-15 1.7535324979386004 1102 16-17 1.6866360121648825 1102 18-19 1.6947013960560042 1102 20-21 1.6763316551705714 1102 22-23 1.6978067872085205 1102 24-25 1.7254334846333634 1102 26-27 1.6672024336337472 1102 28-29 1.495115541477702 1102 30-31 1.815684489133389 1102 32-33 1.8070620156306134 1102 34-35 1.8516472728581874 1102 36-37 1.9301163953043101 1102 38-39 1.7941785791316462 1102 40-41 1.6381297477578656 1102 42-43 1.6451817755246267 1102 44-45 1.7397863500642714 1102 46-47 1.9438621540914411 1102 48-49 1.7010918670261255 1102 50-51 1.8612783408948594 1102 52-53 1.911597994568492 1102 54-55 1.8491978045631186 1102 56-57 1.8537763667072475 1102 58-59 1.8126382414916442 1102 60-61 1.7693565163981013 1102 62-63 1.7759921520499162 1102 64-65 1.7029714948259418 1102 66-67 1.6339925418987775 1102 68-69 1.4718880951606117 1102 70-71 1.6307921807349715 1102 72-73 1.5404377566658276 1102 74-75 1.4203729673997039 1102 76-77 1.4488662525299816 1102 78-79 1.3907821950843307 1102 80-81 1.4152631697845202 1102 82-83 1.2967877490193445 1102 84-85 1.3233987352410388 1102 86-87 0.9687693090335294 1102 88-89 0.4159805155961074 1102 90-91 -5.456866003293399 1102 92-93 -10.293604784940568 1102 94-95 -8.181050210423608 1102 96-97 -4.560755288512269 1102 98-99 -2.88954227694812 1102 100-101 -2.0804309264885603 1103 1 2.0770872063429415 1103 2 1.2521346618461742 1103 3 0.9880096045810021 1103 4 0.8681492839393741 1103 5 0.9747434766015175 1103 6 0.7920520772342243 1103 7 0.7193521251689248 1103 8 0.8596705066560091 1103 9 0.9418765900779533 1103 10-11 0.9382301608818011 1103 12-13 0.9785818777764703 1103 14-15 1.050718357315894 1103 16-17 0.9333344123996952 1103 18-19 0.8746268182804542 1103 20-21 0.8678758371638224 1103 22-23 0.9963535639653855 1103 24-25 0.8444227408176843 1103 26-27 0.8423453453093117 1103 28-29 0.6973684317016478 1103 30-31 0.8027751909288696 1103 32-33 0.8139567850712481 1103 34-35 0.8317397812106151 1103 36-37 0.9342180839021168 1103 38-39 0.8382599841569132 1103 40-41 0.5972542993176759 1103 42-43 0.6920832364724419 1103 44-45 0.6416410965486747 1103 46-47 0.7637263215579821 1103 48-49 0.7616792553631235 1103 50-51 0.676266956051915 1103 52-53 0.7520925905061695 1103 54-55 0.5997656171011769 1103 56-57 0.6133021087141195 1103 58-59 0.7540147608260916 1103 60-61 0.6079239057719157 1103 62-63 0.5376470738934316 1103 64-65 0.5175293758474204 1103 66-67 0.5699447500258366 1103 68-69 0.4815549465983686 1103 70-71 0.373776246079629 1103 72-73 0.5956043726634057 1103 74-75 0.33329958965193285 1103 76-77 0.2847449459480522 1103 78-79 0.04920156509458451 1103 80-81 -0.18436212737492852 1103 82-83 -0.5785501894358518 1103 84-85 -0.6480299212275007 1103 86-87 -0.5324232426008955 1103 88-89 -1.204003115616164 1103 90-91 -6.870200978099003 1103 92-93 -10.146012194795757 1103 94-95 -8.841063430321928 1103 96-97 -5.768071337920677 1103 98-99 -4.396609091619151 1103 100-101 -3.5668687657335845 1104 1 2.003827251319372 1104 2 1.2896362829067343 1104 3 1.0793572942141125 1104 4 0.908887442575967 1104 5 0.9359583749855744 1104 6 0.9483356421540421 1104 7 0.8237916262107703 1104 8 0.9607822179636898 1104 9 1.0353365287503067 1104 10-11 1.0704359126125098 1104 12-13 0.9919863470373613 1104 14-15 1.0519849038508724 1104 16-17 0.9842884722567078 1104 18-19 1.0658666844613478 1104 20-21 1.0606931756118314 1104 22-23 1.0364303380454984 1104 24-25 1.0456407594631258 1104 26-27 1.062185753069116 1104 28-29 0.9780147700447586 1104 30-31 1.1575594857436897 1104 32-33 1.1859408686407917 1104 34-35 1.082462421682088 1104 36-37 1.0388871010352716 1104 38-39 1.0786078981912652 1104 40-41 1.0535295785904353 1104 42-43 1.1357041767193508 1104 44-45 1.0316049119501614 1104 46-47 0.9295432305217517 1104 48-49 0.8382609275118433 1104 50-51 0.8017190903212956 1104 52-53 0.7511309582279999 1104 54-55 0.8341121020753803 1104 56-57 0.8298005437334943 1104 58-59 0.7281553501202183 1104 60-61 0.6284433738635364 1104 62-63 0.7644382958841334 1104 64-65 0.8130099364478589 1104 66-67 0.8845886306332886 1104 68-69 0.6972722158675531 1104 70-71 0.5782397685694356 1104 72-73 0.6085134148434221 1104 74-75 0.5267080497173566 1104 76-77 0.5258976233206063 1104 78-79 0.38194878976962343 1104 80-81 0.19886092900912544 1104 82-83 -0.08070961327322834 1104 84-85 -0.08404363489037436 1104 86-87 0.05630921071765371 1104 88-89 -0.2026992368003384 1104 90-91 -3.0720110353665593 1104 92-93 -7.96007713975958 1104 94-95 -5.828100203352175 1104 96-97 -3.369422428214831 1104 98-99 -2.4459776503233073 1104 100-101 -1.8206809581141634 1105 1 1.9522006816620951 1105 2 1.1743338226035185 1105 3 1.0649910416770894 1105 4 0.816353481367905 1105 5 0.8261510009572035 1105 6 0.8457110396894123 1105 7 0.828441096317313 1105 8 0.8168394758262565 1105 9 0.8969974125468951 1105 10-11 0.7601366340110758 1105 12-13 0.7563325587549699 1105 14-15 0.8146948624710078 1105 16-17 0.6869114430794028 1105 18-19 0.7866274836253808 1105 20-21 0.6828031298336441 1105 22-23 0.6723741329466932 1105 24-25 0.7733707979202151 1105 26-27 0.733133942762386 1105 28-29 0.6513245840872344 1105 30-31 0.9332970411042396 1105 32-33 0.9488632954173042 1105 34-35 0.9612906615731731 1105 36-37 0.9871612140427857 1105 38-39 0.8995593156979282 1105 40-41 0.7468968831818117 1105 42-43 0.9567882538094707 1105 44-45 0.8215710943975338 1105 46-47 0.9664722533881402 1105 48-49 0.7413201142589436 1105 50-51 0.7371752371066442 1105 52-53 0.7038729325005484 1105 54-55 0.6895610412598359 1105 56-57 0.5961877266895037 1105 58-59 0.48350837713524086 1105 60-61 0.5258436007327063 1105 62-63 0.4748764768729501 1105 64-65 0.4617738257998347 1105 66-67 0.4442943108045121 1105 68-69 0.48696456623357065 1105 70-71 0.35438026854708937 1105 72-73 0.32631810446610743 1105 74-75 0.31670970264157816 1105 76-77 0.3663255249652302 1105 78-79 0.09814205562864942 1105 80-81 0.15823942950180836 1105 82-83 -0.2682973583653592 1105 84-85 -0.2826165866654229 1105 86-87 0.03618929212948885 1105 88-89 -0.12076349937266784 1105 90-91 -3.297961874704452 1105 92-93 -8.091388845087884 1105 94-95 -5.87282031646043 1105 96-97 -3.148703129508723 1105 98-99 -2.044606858274653 1105 100-101 -1.4530862182439996 1106 1 1.7258353511159186 1106 2 1.1544398743384434 1106 3 0.8446058605060607 1106 4 0.8551988442206024 1106 5 0.873888375878181 1106 6 0.9368744807274751 1106 7 0.8146868004579275 1106 8 0.02385016742974244 1106 9 -0.28825707436485715 1106 10-11 -0.48837015005005924 1106 12-13 -0.14017122415125982 1106 14-15 0.7033953564143403 1106 16-17 0.8787104763730795 1106 18-19 0.9254708602793755 1106 20-21 0.9090143131470683 1106 22-23 0.8306582099256801 1106 24-25 0.7833753631415377 1106 26-27 0.9306546810836167 1106 28-29 0.7925901690793467 1106 30-31 0.9060772938484547 1106 32-33 0.9557472302486545 1106 34-35 1.0500904827979483 1106 36-37 0.9074142306037416 1106 38-39 1.004384811446407 1106 40-41 0.9823099559166266 1106 42-43 1.12662263996544 1106 44-45 0.9357385542788066 1106 46-47 0.9724920400036225 1106 48-49 0.806265905996117 1106 50-51 0.8462965380338296 1106 52-53 0.8518386422111881 1106 54-55 0.9606547207549312 1106 56-57 1.03724851638216 1106 58-59 1.0589866379520139 1106 60-61 0.8003101751986996 1106 62-63 0.903536491393016 1106 64-65 0.8194852834298594 1106 66-67 0.8079411632888842 1106 68-69 0.8000881616105495 1106 70-71 0.7787358912652635 1106 72-73 0.9777435077652719 1106 74-75 0.6979985530458173 1106 76-77 0.576886373268092 1106 78-79 0.6451253472308522 1106 80-81 0.49529218731918334 1106 82-83 0.34467898943416486 1106 84-85 0.21178007794319953 1106 86-87 0.3725561608954884 1106 88-89 -0.07199729087462714 1106 90-91 -3.6851936224166266 1106 92-93 -8.743305420579501 1106 94-95 -6.214523923314864 1106 96-97 -3.4201857440036463 1106 98-99 -2.305161953973652 1106 100-101 -1.6724897488986574 1107 1 1.8168689765418868 1107 2 1.0994973800759773 1107 3 0.8322090057178286 1107 4 0.8091549173133075 1107 5 0.6737766678317243 1107 6 0.8001486566089682 1107 7 0.6024322104308055 1107 8 0.6556730949629568 1107 9 0.6112893996597677 1107 10-11 0.6658944676215484 1107 12-13 0.7016870848410193 1107 14-15 0.7470786141165533 1107 16-17 0.75700079993522 1107 18-19 0.6881522445898298 1107 20-21 0.6779655397622761 1107 22-23 0.6548765795967242 1107 24-25 0.5788218475980358 1107 26-27 0.6717784553377371 1107 28-29 0.6197440914661598 1107 30-31 0.7412761298245272 1107 32-33 0.9705119261593538 1107 34-35 1.0050729876661606 1107 36-37 0.6548926931185193 1107 38-39 0.5384217533408346 1107 40-41 0.5729811821841011 1107 42-43 0.706095873500459 1107 44-45 0.5738830271211484 1107 46-47 0.5006896285007372 1107 48-49 0.5562754033533714 1107 50-51 0.5080282735509023 1107 52-53 0.6244828412166861 1107 54-55 0.5770884957045084 1107 56-57 0.5858410601960493 1107 58-59 0.41952620520286743 1107 60-61 0.3045843554268082 1107 62-63 0.3364764993907947 1107 64-65 0.4059881913163572 1107 66-67 0.45432964649355867 1107 68-69 0.5423733083523281 1107 70-71 0.45523445253516925 1107 72-73 0.4461051593057128 1107 74-75 0.351677203334436 1107 76-77 0.35719241854245 1107 78-79 0.27423696399836217 1107 80-81 0.19090647290927976 1107 82-83 -0.05607569187190364 1107 84-85 0.11029516504939707 1107 86-87 0.37409751460576146 1107 88-89 0.23780248223198797 1107 90-91 -3.045224831123768 1107 92-93 -4.514623771125436 1107 94-95 -2.9395001690551794 1107 96-97 -1.3453741698329793 1107 98-99 -0.8883249868688488 1107 100-101 -0.5858363611456827 1108 1 1.6601812251470491 1108 2 1.0325255525166135 1108 3 -0.3622058930754761 1108 4 -0.31606687780801224 1108 5 0.3687171440222059 1108 6 0.5284619594505386 1108 7 0.5627655113260914 1108 8 0.6234099737744216 1108 9 0.8048019531825332 1108 10-11 0.6535335051847966 1108 12-13 0.6454835523380993 1108 14-15 0.6046903226282936 1108 16-17 0.6346404051642338 1108 18-19 0.5027486342356156 1108 20-21 0.4422882739649481 1108 22-23 0.4371510135528709 1108 24-25 0.3489756039234564 1108 26-27 0.42681689441909043 1108 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1.1533357799695168 2311 84-85 1.3585358328893413 2311 86-87 1.709204421484852 2311 88-89 2.11280769249505 2311 90-91 2.8072472264284087 2311 92-93 3.7699272585769243 2311 94-95 3.387868030586663 2311 96-97 2.67835105247158 2311 98-99 2.442563459469305 2311 100-101 2.2796734341488705 2312 1 1.2367326185983707 2312 2 0.6092396541695315 2312 3 -0.23270281562352224 2312 4 -0.6119048312620592 2312 5 -0.10215051921818485 2312 6 -0.2602081675503385 2312 7 0.05545658578304824 2312 8 0.11632024725802381 2312 9 0.17722237918258799 2312 10-11 0.09826701364822554 2312 12-13 0.12768358587427286 2312 14-15 0.15738766994346776 2312 16-17 0.01035168680979126 2312 18-19 -0.36576014034347537 2312 20-21 0.300198675055654 2312 22-23 0.440250879020617 2312 24-25 0.4984489375848895 2312 26-27 0.5162235520876877 2312 28-29 0.47954378149057675 2312 30-31 0.35963030431886267 2312 32-33 0.528890366418608 2312 34-35 0.4337353765132974 2312 36-37 0.4660199748831815 2312 38-39 0.34126466382364384 2312 40-41 0.3319946027176286 2312 42-43 0.3974931510381836 2312 44-45 0.21801865709171864 2312 46-47 0.3439572485771336 2312 48-49 0.49069899359071556 2312 50-51 0.32174097472192287 2312 52-53 0.2471002366390671 2312 54-55 0.2594127646004907 2312 56-57 0.4214822047573854 2312 58-59 0.470829022379899 2312 60-61 0.36600928419504797 2312 62-63 0.36507483836004795 2312 64-65 0.4143941066836341 2312 66-67 0.60048031341071 2312 68-69 0.551273403849855 2312 70-71 0.6657179870395247 2312 72-73 0.5722804638019205 2312 74-75 0.6972601491982964 2312 76-77 0.8871190341727342 2312 78-79 0.8937486546176849 2312 80-81 0.8900546075764453 2312 82-83 0.9662428432586623 2312 84-85 1.4659971305763584 2312 86-87 1.620407905049749 2312 88-89 1.981882711895711 2312 90-91 2.7911785911318496 2312 92-93 3.741816186761721 2312 94-95 3.4406632921822187 2312 96-97 2.598000689269032 2312 98-99 2.345068697770138 2312 100-101 2.3423171119897663 2313 1 -7.32909246670604 2313 2 -2.7066103529355345 2313 3 -1.591349989826174 2313 4 -0.8688060772404782 2313 5 -0.6159521042935339 2313 6 -0.3190903426483658 2313 7 -0.23503112042697438 2313 8 -0.1865158362166497 2313 9 -0.02829517145052307 2313 10-11 -0.1760045529874361 2313 12-13 -0.36732122373677356 2313 14-15 -0.4497391000188671 2313 16-17 -0.5631075442286644 2313 18-19 -0.8323989529593661 2313 20-21 -0.5805015895235286 2313 22-23 -1.2000519001037304 2313 24-25 -0.3954911861932757 2313 26-27 -0.4028896657168488 2313 28-29 -0.585017791192584 2313 30-31 -0.14547257824577287 2313 32-33 -0.265163319848817 2313 34-35 -0.27846763462620316 2313 36-37 -0.08923858086340886 2313 38-39 -0.11386484617643333 2313 40-41 -0.10126443144383046 2313 42-43 -0.18407408874734443 2313 44-45 -0.18941588054069314 2313 46-47 -1.195360702604603 2313 48-49 -0.6950063533615669 2313 50-51 -0.6944383838324377 2313 52-53 -1.0948366522230977 2313 54-55 -0.7459497825477541 2313 56-57 -0.4577433484560096 2313 58-59 -0.5506408560052023 2313 60-61 -0.343235880679142 2313 62-63 -0.360244242656492 2313 64-65 -0.4297487888173208 2313 66-67 -0.29999267062489565 2313 68-69 -0.2604804637729323 2313 70-71 -0.10296786984383388 2313 72-73 -0.15056255173525912 2313 74-75 -0.04244200232220763 2313 76-77 0.15738945716109143 2313 78-79 -0.011940432647264743 2313 80-81 0.13145213914271991 2313 82-83 0.4126296321162144 2313 84-85 0.5255656579940684 2313 86-87 1.0050109166357153 2313 88-89 1.427195700761505 2313 90-91 2.039904980734132 2313 92-93 3.149072888424012 2313 94-95 2.806658351491919 2313 96-97 1.7285640496248753 2313 98-99 1.465360648729618 2313 100-101 1.3118665955940134 2314 1 0.5944907151308385 2314 2 -0.07887187548165286 2314 3 -1.3135096333630614 2314 4 -0.3448512085351254 2314 5 -0.14944356692760152 2314 6 -3.507309427642852 2314 7 -2.8645141280709723 2314 8 -2.4450449992521293 2314 9 -3.2933297391409084 2314 10-11 -2.5518647912052828 2314 12-13 -1.1454784064722574 2314 14-15 -1.0819827572328506 2314 16-17 -2.042738384478504 2314 18-19 -3.117373979023096 2314 20-21 -2.8991734282618893 2314 22-23 -4.162176844495683 2314 24-25 -3.160310414684794 2314 26-27 -1.9725716754300606 2314 28-29 -1.7529719089074618 2314 30-31 -1.9767261780713525 2314 32-33 -2.179197057121044 2314 34-35 -1.5196308193890786 2314 36-37 -1.9174074384276558 2314 38-39 -3.2944783143782033 2314 40-41 -2.5701371042907795 2314 42-43 -1.5681799609226061 2314 44-45 -2.091446655611275 2314 46-47 -1.6269150246532575 2314 48-49 -2.1390643788559807 2314 50-51 -3.0495587882213115 2314 52-53 -1.9827639323993544 2314 54-55 -2.4119270874575847 2314 56-57 -1.4735796501532867 2314 58-59 -0.8798899127060942 2314 60-61 -1.2618446524503923 2314 62-63 -0.8995714721516457 2314 64-65 -1.337694498296944 2314 66-67 -0.6954816742611598 2314 68-69 -0.6843137907821131 2314 70-71 -0.7200596387843383 2314 72-73 -1.809875735363942 2314 74-75 -1.8509269633322312 2314 76-77 -1.0267898110484346 2314 78-79 -1.8930101209695707 2314 80-81 -0.905906081577303 2314 82-83 -0.43568671606394815 2314 84-85 -1.6276009329330385 2314 86-87 -0.29671717653316065 2314 88-89 0.9362434849407464 2314 90-91 2.2041237428845726 2314 92-93 3.2850438023536093 2314 94-95 3.0346114467054512 2314 96-97 2.119538612718859 2314 98-99 1.9740077393283002 2314 100-101 1.9707758773187312 2315 1 -2.775069457255128 2315 2 -1.9210812520724012 2315 3 -2.6976876326129293 2315 4 -3.9004358219963287 2315 5 -4.017957618618556 2315 6 -3.108934612324571 2315 7 -5.695189505179705 2315 8 -3.045804558308557 2315 9 -2.5970935215148856 2315 10-11 -3.8628299334603717 2315 12-13 -2.993731191820494 2315 14-15 -2.0724298544563524 2315 16-17 -2.489515654212127 2315 18-19 -2.069024146445713 2315 20-21 -1.3916057186096964 2315 22-23 -0.8708197431750406 2315 24-25 -2.2114409035611615 2315 26-27 -3.963625331370146 2315 28-29 -3.3196511944689284 2315 30-31 -3.221681591710759 2315 32-33 -4.080079746947291 2315 34-35 -3.808409270190616 2315 36-37 -3.020883807396679 2315 38-39 -1.867758211883281 2315 40-41 -1.3698516037509876 2315 42-43 -2.907778096102838 2315 44-45 -6.145479736918006 2315 46-47 -3.0527219505822103 2315 48-49 -2.194742596389027 2315 50-51 -3.251224366840649 2315 52-53 -3.2762637133943393 2315 54-55 -1.7948969499420677 2315 56-57 -1.3482523517050318 2315 58-59 -2.4695395147868275 2315 60-61 -2.8406521528163644 2315 62-63 -4.797213930799831 2315 64-65 -4.458392121666641 2315 66-67 -3.762532795546626 2315 68-69 -2.1504110167378307 2315 70-71 -3.498051063951177 2315 72-73 -2.4616178249807383 2315 74-75 -1.4964060977989995 2315 76-77 -3.148283817933592 2315 78-79 -1.612318452024308 2315 80-81 -1.0578568900479617 2315 82-83 -0.690549706555057 2315 84-85 -2.2806123890464285 2315 86-87 -1.711495929495218 2315 88-89 -0.9914358292054182 2315 90-91 -0.11383057558538567 2315 92-93 0.2269789787457661 2315 94-95 0.08216601461303341 2315 96-97 -0.16230326565987596 2315 98-99 -0.24913191628667164 2315 100-101 -0.4925910945305372 2316 1 -7.479739058829125 2316 2 -6.359184136648359 2316 3 -3.4358770669465315 2316 4 -4.212808990798958 2316 5 -2.1566851737676593 2316 6 -2.958114751695529 2316 7 -3.25592371036889 2316 8 -2.4885139965403127 2316 9 -7.045091319294485 2316 10-11 -6.812262130802139 2316 12-13 -5.987146777135145 2316 14-15 -4.765463557163727 2316 16-17 -4.759188045701073 2316 18-19 -4.093063393626238 2316 20-21 -2.782600228211578 2316 22-23 -3.274273867346757 2316 24-25 -3.1227448575153787 2316 26-27 -4.0460247934127445 2316 28-29 -4.1443000261809 2316 30-31 -6.734971347107418 2316 32-33 -4.8493812639598275 2316 34-35 -5.377900027520305 2316 36-37 -4.2081122809299245 2316 38-39 -6.335708989642637 2316 40-41 -3.7970811736405885 2316 42-43 -4.663105154950138 2316 44-45 -3.215364618015336 2316 46-47 -4.22729359910403 2316 48-49 -5.470024937861542 2316 50-51 -4.8169032431734315 2316 52-53 -6.373923661090121 2316 54-55 -5.2708829575826925 2316 56-57 -4.511994027270308 2316 58-59 -3.1985852182880983 2316 60-61 -3.3664643998596198 2316 62-63 -4.235522010797599 2316 64-65 -3.0483537685067894 2316 66-67 -3.94973801978864 2316 68-69 -3.1330089965304104 2316 70-71 -2.680154220090742 2316 72-73 -2.2376930192794475 2316 74-75 -2.343525963716697 2316 76-77 -2.8725334080627007 2316 78-79 -4.965424824736743 2316 80-81 -5.930424145122249 2316 82-83 -3.455386654001856 2316 84-85 -2.0922837709367634 2316 86-87 -2.0671652208805895 2316 88-89 -1.0706540008514445 2316 90-91 -0.8775446119772887 2316 92-93 -0.956758647134663 2316 94-95 -1.526323386087089 2316 96-97 -2.9929027646042226 2316 98-99 -4.334413510513599 2316 100-101 -3.697083838077633 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38007.0 3 13170.0 4 13244.0 5 14966.0 6 16581.0 7 19269.0 8 22900.0 9 27204.0 10 31360.0 11 36373.0 12 41735.0 13 45673.0 14 49339.0 15 54268.0 16 59632.0 17 64281.0 18 63780.0 19 65579.0 20 71583.0 21 78538.0 22 87542.0 23 98723.0 24 110707.0 25 124874.0 26 140830.0 27 161458.0 28 182112.0 29 206669.0 30 235283.0 31 264935.0 32 296518.0 33 330459.0 34 374557.0 35 428766.0 36 446886.0 37 328194.0 38 85615.0 39 1194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72222275116256 18.636070218733757 12.739866508153828 23.90184052194986 2 16.431570714841328 19.315403578151997 39.0223810612774 25.23064464572927 3 19.988287955507772 23.09191627749929 28.4978837503422 28.421912016650737 4 13.501453794061852 15.293253388412895 35.32838057370594 35.87691224381931 5 14.838887933345223 36.44503483644231 33.29353899453817 15.422538235674303 6 34.36016480423995 34.19167464365908 16.838901993444054 14.60925855865692 7 30.359906100961148 31.298388515210334 19.855210408222916 18.486494975605595 8 27.4004509383403 34.30015884846922 19.132373301098056 19.167016912092418 9 27.225033043761687 16.053373744257687 19.09689203446922 37.624701177511405 10-11 26.169904247685555 25.32997760555405 26.600597601446164 21.89952054531423 12-13 26.74744246647321 22.51423062166957 27.029780031907404 23.70854687994982 14-15 23.67244606789831 24.037363423169644 24.76734627193755 27.522844236994498 16-17 23.760538760068446 27.259793826868023 25.32958043453396 23.650086978529565 18-19 23.88112629249373 25.949624213953115 27.086204909616313 23.083044583936836 20-21 24.438364885903507 25.257126275154246 27.11054146557717 23.19396737336508 22-23 24.053503727928742 25.10471103311922 27.133920022832953 23.707865216119085 24-25 23.82959221879802 25.33559271350952 27.18388866958185 23.65092639811061 26-27 23.594782745723982 25.670604960377723 27.045163506754662 23.689448787143633 28-29 23.490695132393714 25.9203047466717 27.074393405062438 23.514606715872144 30-31 23.73003000958388 25.62136124133218 26.936261956422413 23.712346792661528 32-33 23.595409009509645 25.626976940006063 26.93833302555049 23.839281024933797 34-35 23.91767424196143 25.69200271597203 26.936466773652445 23.4538562684141 36-37 24.04283433044543 25.493675579914544 26.800596393073477 23.66289369656655 38-39 23.655109795079674 25.701761481348473 26.783524216239474 23.859604507332378 40-41 23.967388237331964 25.51952060882518 26.937801733078253 23.5752894207646 42-43 23.769596503053993 25.5139934904445 26.825507048383574 23.890902958117934 44-45 23.60964490499196 25.628880682369154 26.97330170986099 23.788172702777892 46-47 23.800068292088973 25.82292231907229 26.59358530820114 23.783424080637605 48-49 23.726595181844615 25.87676414933962 26.480503200611366 23.9161374682044 50-51 23.63811287343638 26.027298594142422 26.511241024387356 23.823347508033844 52-53 23.771451971385048 25.78889774054082 26.502131864050916 23.93751842402322 54-55 23.530887803505728 25.934275238406745 26.546360303899753 23.988476654187775 56-57 23.391558225439223 26.022982512412586 26.524325190362287 24.061134071785908 58-59 23.486206180850882 25.725673912675152 26.439254672065875 24.348865234408095 60-61 23.356459169322513 25.816002189252863 26.739733510336755 24.087805131087865 62-63 23.338267273474614 25.79704410303117 26.716825392187904 24.14786323130631 64-65 23.155100785224757 25.801362631397428 26.62673888360428 24.41679769977353 66-67 23.21276031055337 25.813317140196297 26.51008995681442 24.463832592435914 68-69 23.221657659015772 25.582476643132303 26.73382635101001 24.462039346841916 70-71 23.132591602111912 25.550586683351913 26.550865123704803 24.765956590831372 72-73 23.127924873309542 25.731365931257844 26.415443023952438 24.72526617148017 74-75 22.932847543415654 25.889060364643214 26.463957189710552 24.714134902230576 76-77 23.134164888389588 25.499709452157905 26.531932828474243 24.834192830978267 78-79 23.13647433679235 25.620130147652986 26.535296701462315 24.70809881409235 80-81 23.125325684529425 25.665302185983442 26.474000906593435 24.735371222893697 82-83 23.077739583850864 25.621544100814454 26.47133631650631 24.82937999882837 84-85 22.967505742538354 25.596126263509355 26.36484110307148 25.071526890880808 86-87 22.814854715515573 25.42623044923521 26.79329859336379 24.96561624188542 88-89 22.919981316864497 25.383613280277746 26.582606678691374 25.11379872416638 90-91 22.674059538574774 25.331431314672244 26.895407650011588 25.0991014967414 92-93 22.81883424179594 24.99405398914096 27.049556466911167 25.137555302151938 94-95 22.716355213055937 24.956957566484007 27.036828591306833 25.289858629153223 96-97 23.07976825649694 24.727775973086867 26.589517719486615 25.60293805092958 98-99 22.96753643641733 24.78548624654215 26.460252014512562 25.78672530252796 100-101 23.033567974711524 24.744409445229763 26.551301764077024 25.67072081598169 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5793.0 1 3317.5 2 819.0 3 761.0 4 644.5 5 591.5 6 716.0 7 1086.5 8 1591.0 9 1870.5 10 1813.0 11 1697.0 12 1774.0 13 1939.5 14 2168.5 15 2449.0 16 2784.5 17 3077.5 18 3414.5 19 3940.5 20 4619.0 21 5405.5 22 6483.0 23 7837.5 24 9652.0 25 12325.5 26 15780.5 27 20204.0 28 25021.0 29 30093.0 30 36012.0 31 43617.5 32 52591.5 33 58354.0 34 62271.5 35 68966.5 36 78199.5 37 87863.0 38 96768.0 39 103698.0 40 110658.0 41 120046.0 42 128587.0 43 137652.5 44 147449.5 45 156517.0 46 165108.0 47 174537.0 48 184322.0 49 190247.5 50 192665.0 51 194800.5 52 197183.5 53 198446.0 54 196898.5 55 189599.5 56 177835.0 57 164626.5 58 151948.0 59 136476.5 60 117027.5 61 98314.0 62 81038.0 63 64078.5 64 48925.0 65 37078.5 66 27618.5 67 20661.5 68 15147.0 69 10841.0 70 8112.0 71 6048.0 72 4462.5 73 3411.0 74 2611.0 75 2000.5 76 1471.0 77 1039.5 78 688.0 79 456.0 80 321.5 81 219.0 82 153.0 83 84.0 84 56.0 85 52.0 86 42.5 87 30.0 88 18.0 89 15.5 90 15.0 91 12.5 92 8.0 93 6.5 94 6.0 95 4.5 96 3.5 97 2.0 98 2.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13465590377290082 2 0.12100649002156015 3 0.12774668040341414 4 0.13839575862427433 5 0.1267958698479802 6 0.14181867662383651 7 0.17230800176808506 8 0.15944036558454566 9 0.16922314974378824 10-11 0.1637190130839984 12-13 0.15329179065940612 14-15 0.15820431186248152 16-17 0.1523938029126074 18-19 0.16849419498462223 20-21 0.1738609923419605 22-23 0.2071921845907838 24-25 0.2294622807113922 26-27 0.2423510460183857 28-29 0.2375547349943078 30-31 0.22845864734732305 32-33 0.2294622807113922 34-35 0.21909844565716222 36-37 0.22714864169316962 38-39 0.2152635097502453 40-41 0.21969006111387668 42-43 0.21244277176912463 44-45 0.20558637120827317 46-47 0.22061974254585656 48-49 0.2582295822941326 50-51 0.2599832995408219 52-53 0.26209621188623067 54-55 0.2598882184852785 56-57 0.23418463980338083 58-59 0.23504036930327138 60-61 0.20561806489345427 62-63 0.19858206678324308 64-65 0.19952231277694998 66-67 0.20739291126359766 68-69 0.2066850856278857 70-71 0.2127491440592089 72-73 0.24409419870334795 74-75 0.2471262279190095 76-77 0.26223355118868225 78-79 0.25867329388666843 80-81 0.2733580346872594 82-83 0.2722593202676468 84-85 0.2467353391351089 86-87 0.24985188484458687 88-89 0.25289447862197545 90-91 0.44303546058531057 92-93 0.5909604538873784 94-95 0.3430841420857487 96-97 0.2877997905681283 98-99 0.31903919959499694 100-101 0.3387315426542067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4732804.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.64047653464164 #Duplication Level Percentage of deduplicated Percentage of total 1 70.93151648018356 32.37348213480365 2 13.190030497071797 12.039985547856261 3 5.646326291510665 7.731030678438682 4 3.0103082180351723 5.495676063490928 5 1.830925525014002 4.1782156730538995 6 1.1713313579304205 3.2076072813547976 7 0.8188994693259785 2.6162473409801974 8 0.5813498480266036 2.1226467277820555 9 0.4218183713726115 1.7326792332461187 >10 2.199362348940617 17.590066781905943 >50 0.13169299060334655 4.132468826795319 >100 0.06269482363422658 4.953857616095913 >500 0.0025590387173774288 0.7822286338020984 >1k 0.001089960463060287 0.7547597417720441 >5k 9.477917070082603E-5 0.2890477186220958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7803 0.1648705503122462 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5561 0.1174990555281816 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.451649381635073E-5 2 0.0 0.0 0.0 0.0 8.451649381635073E-5 3 0.0 0.0 0.0 0.0 8.451649381635073E-5 4 0.0 0.0 0.0 0.0 8.451649381635073E-5 5 0.0 2.1129123454087682E-5 0.0 0.0 8.451649381635073E-5 6 0.0 2.1129123454087682E-5 0.0 0.0 8.451649381635073E-5 7 0.0 2.1129123454087682E-5 0.0 0.0 8.451649381635073E-5 8 0.0 2.1129123454087682E-5 0.0 0.0 8.451649381635073E-5 9 0.0 2.1129123454087682E-5 0.0 0.0 8.451649381635073E-5 10-11 0.0 2.1129123454087682E-5 0.0 0.0 1.2677474072452608E-4 12-13 0.0 2.1129123454087682E-5 0.0 0.0 2.324203579949645E-4 14-15 0.0 3.169368518113152E-5 0.0 0.0 2.7467860490313983E-4 16-17 0.0 4.2258246908175364E-5 0.0 0.0 3.380659752654029E-4 18-19 0.0 4.2258246908175364E-5 0.0 4.2258246908175364E-5 4.01453345627666E-4 20-21 0.0 6.338737036226304E-5 0.0 1.3733930245156994E-4 4.331470308087975E-4 22-23 0.0 6.338737036226304E-5 0.0 3.380659752654029E-4 4.64840715989929E-4 24-25 0.0 6.338737036226304E-5 0.0 8.66294061617595E-4 4.859698394440167E-4 26-27 0.0 8.451649381635073E-5 0.0 0.0018065400553244969 4.965344011710605E-4 28-29 0.0 8.451649381635073E-5 0.0 0.0069937398633030225 5.070989628981043E-4 30-31 0.0 8.451649381635073E-5 0.0 0.026252935891703945 5.176635246251482E-4 32-33 2.1129123454087682E-5 1.1621017899748226E-4 0.0 0.0717122450031736 6.233091418955867E-4 34-35 2.1129123454087682E-5 1.2677474072452608E-4 0.0 0.14148061064857112 6.972610739848935E-4 36-37 2.1129123454087682E-5 1.2677474072452608E-4 0.0 0.2596135398803754 6.972610739848935E-4 38-39 4.2258246908175364E-5 1.2677474072452608E-4 0.0 0.4407957734991772 7.500838826201128E-4 40-41 4.2258246908175364E-5 1.2677474072452608E-4 0.0 0.6430754368868856 7.606484443471566E-4 42-43 4.2258246908175364E-5 1.2677474072452608E-4 0.0 0.849179471619784 7.606484443471566E-4 44-45 4.2258246908175364E-5 1.2677474072452608E-4 0.0 1.0945625468538314 7.923421295282881E-4 46-47 4.2258246908175364E-5 1.2677474072452608E-4 0.0 1.3773019123547057 8.240358147094196E-4 48-49 4.2258246908175364E-5 1.2677474072452608E-4 0.0 1.6782545822730035 8.557294998905512E-4 50-51 4.2258246908175364E-5 1.2677474072452608E-4 0.0 2.003484615040048 8.874231850716826E-4 52-53 4.2258246908175364E-5 1.2677474072452608E-4 0.0 2.3126987722288943 9.402459937069018E-4 54-55 6.338737036226304E-5 1.2677474072452608E-4 0.0 2.6486835288340695 9.508105554339458E-4 56-57 6.338737036226304E-5 1.2677474072452608E-4 0.0 3.025616526693267 9.508105554339458E-4 58-59 6.338737036226304E-5 1.2677474072452608E-4 0.0 3.4292989948453387 9.508105554339458E-4 60-61 6.338737036226304E-5 1.2677474072452608E-4 0.0 3.8506242810815747 9.825042406150773E-4 62-63 6.338737036226304E-5 1.2677474072452608E-4 0.0 4.265631959405038 9.93068802342121E-4 64-65 6.338737036226304E-5 1.2677474072452608E-4 0.0 4.69929665373846 0.0010141979257962087 66-67 6.338737036226304E-5 1.4790386417861377E-4 0.0 5.177163474337835 0.0010247624875232525 68-69 6.338737036226304E-5 1.6903298763270146E-4 0.0 5.695936277944322 0.001056456172704384 70-71 6.338737036226304E-5 1.7959754935974528E-4 0.0 6.2350986856840045 0.0010987144196125593 72-73 6.338737036226304E-5 1.9016211108678914E-4 0.0 6.773743429899062 0.0011198435430666471 74-75 6.338737036226304E-5 1.9016211108678914E-4 0.0 7.3112260723241445 0.001130408104793691 76-77 6.338737036226304E-5 1.9016211108678914E-4 0.0 7.873672774110231 0.0012043600368829978 78-79 6.338737036226304E-5 1.9016211108678914E-4 0.0 8.481124508853526 0.001267747407245261 80-81 6.338737036226304E-5 1.9016211108678914E-4 0.0 9.098496367058512 0.0012888765306993486 82-83 6.338737036226304E-5 2.00726672813833E-4 0.0 9.69046045430996 0.0013522639010616116 84-85 7.395193208930688E-5 2.1129123454087683E-4 0.0 10.259710733848264 0.0013733930245156995 86-87 8.451649381635073E-5 2.1129123454087683E-4 0.0 10.827978509145952 0.0014367803948779625 88-89 8.451649381635073E-5 2.1129123454087683E-4 2.1129123454087682E-5 11.433433541722835 0.0015001677652402255 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5625 0.0 48.073517 1 GTATCAA 10675 0.0 42.308014 1 TCAACGC 13580 0.0 33.95879 4 TATCAAC 13575 0.0 33.934555 2 ATCAACG 13735 0.0 33.6443 3 CAACGCA 13880 0.0 33.225163 5 AACGCAG 14845 0.0 31.416592 6 ACGCAGA 17235 0.0 26.702147 7 TACATGG 17100 0.0 26.578602 2 CGCAGAG 17705 0.0 25.93944 8 GTACATG 17360 0.0 25.85205 1 ACATGGG 17505 0.0 24.718029 3 GCAGAGT 19345 0.0 22.317688 9 CATGGGG 11595 0.0 20.459028 4 AGAGTAC 16680 0.0 19.909279 10-11 GAGTACA 12835 0.0 19.58996 1 GAGTACT 9375 0.0 19.027393 12-13 CAGAGTA 17785 0.0 18.912369 10-11 AGTACTT 9750 0.0 18.757826 12-13 GGACCGT 625 0.0 18.218683 6 >>END_MODULE