FastQCFastQC Report
Fri 27 May 2016
SRR939865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732804
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89280.18864081419809484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69980.14786160593170558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49020.10357496317193783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61600.049.2389561
GTATCAA112500.042.852581
TATCAAC146200.032.80482
TCAACGC145450.032.7738724
ATCAACG146750.032.5505563
CAACGCA149000.031.9930175
AACGCAG156650.030.471196
ACGCAGA184600.025.7299677
CGCAGAG187200.025.271928
TACATGG178600.024.5564352
GTACATG183250.024.480211
ACATGGG181400.022.927593
GCAGAGT209300.021.9055429
GTGGTAT38200.020.6220441
AGAGTAC185600.019.68857210-11
TGGTATC34600.019.6268312
AGTACTT107650.018.93188712-13
CATGGGG117850.018.8043614
GAGTACT103850.018.76250612-13
CAGAGTA194250.018.67841710-11
Produced by FastQC (version 0.11.5)