FastQCFastQC Report
Fri 27 May 2016
SRR939864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4993453
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.17014278496263008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59460.11907591800703841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.048.3480831
GTATCAA115200.044.3113061
TATCAAC148000.034.1737632
TCAACGC149100.033.891794
ATCAACG150300.033.558253
CAACGCA151800.033.1025
AACGCAG159350.031.7701536
ACGCAGA184550.027.075077
GTACATG182450.026.7148781
TACATGG180200.026.2134972
CGCAGAG190700.026.004798
ACATGGG187700.024.1142773
GCAGAGT205250.022.7859259
CATGGGG123650.020.6620544
AGAGTAC177350.020.2151210-11
GAGTACT100350.019.12961612-13
GAGTACA137550.018.9832121
CAGAGTA191650.018.96453710-11
AGTACTT107600.018.49658412-13
TGGTATC33000.018.1065562
Produced by FastQC (version 0.11.5)