##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4993453 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.130572371463195 31.0 30.0 33.0 26.0 34.0 2 30.20143616050857 31.0 30.0 33.0 26.0 34.0 3 30.167182709039217 31.0 30.0 34.0 26.0 34.0 4 33.81318658651639 35.0 35.0 37.0 28.0 37.0 5 33.66149976779595 35.0 33.0 37.0 28.0 37.0 6 33.63252442748535 35.0 33.0 37.0 28.0 37.0 7 33.619829004097966 35.0 33.0 37.0 28.0 37.0 8 33.45303961006542 35.0 33.0 37.0 27.0 37.0 9 34.77412644116206 37.0 34.0 39.0 27.0 39.0 10-11 34.569269701747466 37.0 34.0 39.0 27.0 39.0 12-13 34.46747551243598 37.0 33.0 39.0 27.0 39.0 14-15 35.10470269771238 38.0 33.0 40.0 26.5 41.0 16-17 34.87879579521425 38.0 33.0 40.0 25.5 41.0 18-19 34.850249516717184 38.0 33.5 40.0 25.0 41.0 20-21 34.93940335475271 38.0 33.5 40.0 25.0 41.0 22-23 34.84193522999015 38.0 33.0 40.0 25.0 41.0 24-25 34.68648338133953 38.0 33.0 40.0 25.0 41.0 26-27 34.58086718749531 38.0 33.0 40.0 24.0 41.0 28-29 34.4616809250032 38.0 33.0 40.0 24.0 41.0 30-31 34.28426721949721 38.0 33.0 40.0 23.5 41.0 32-33 34.03207529939703 38.0 33.0 40.0 23.0 41.0 34-35 33.76027740723704 38.0 32.0 40.0 21.0 41.0 36-37 33.5641672205586 38.0 32.0 40.0 20.5 41.0 38-39 33.28484467561825 37.0 31.0 40.0 18.5 41.0 40-41 32.985986050134045 37.0 31.0 40.0 17.0 41.0 42-43 32.68116061170497 37.0 31.0 40.0 15.5 41.0 44-45 32.29037441626065 37.0 30.0 39.5 14.5 41.0 46-47 31.93820628731261 36.5 30.0 39.0 10.5 40.5 48-49 31.603728021471312 36.0 30.0 39.0 9.0 40.0 50-51 30.834113388070342 35.5 28.5 38.5 8.0 40.0 52-53 30.92760610743708 35.0 29.0 38.5 7.5 40.0 54-55 30.99884208382456 35.5 29.0 39.0 6.5 40.0 56-57 30.75302220727821 35.0 28.5 39.0 2.0 40.0 58-59 30.193296302178073 35.0 28.0 38.0 2.0 40.0 60-61 29.67653946076993 34.0 27.0 38.0 2.0 40.0 62-63 29.221049842663984 34.0 26.0 38.0 2.0 40.0 64-65 28.685406771626766 34.0 26.0 37.0 2.0 39.5 66-67 28.13749493586903 33.0 25.5 36.5 2.0 39.0 68-69 27.58885054089825 33.0 25.0 36.0 2.0 39.0 70-71 27.04064431967218 32.0 23.5 35.5 2.0 38.5 72-73 26.565761107594284 32.0 22.0 35.0 2.0 38.0 74-75 25.970750100181178 31.5 21.0 35.0 2.0 37.0 76-77 25.324299137290367 31.0 19.0 34.5 2.0 36.5 78-79 24.680605384690715 31.0 17.5 34.0 2.0 36.0 80-81 24.013989718136926 30.0 11.5 34.0 2.0 35.5 82-83 23.27474795497224 30.0 7.0 34.0 2.0 35.0 84-85 22.533783035506694 29.0 2.0 34.0 2.0 35.0 86-87 21.741962525731193 29.0 2.0 34.0 2.0 35.0 88-89 20.879994865276593 27.5 2.0 33.0 2.0 35.0 90-91 19.909540752661535 26.0 2.0 33.0 2.0 34.0 92-93 18.916511380000976 25.0 2.0 32.0 2.0 34.0 94-95 17.532489041150484 22.0 2.0 32.0 2.0 34.0 96-97 14.980070904842801 4.5 2.0 31.0 2.0 34.0 98-99 13.585735762407296 2.0 2.0 30.0 2.0 34.0 100-101 12.30833082838669 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8339661729172754 1101 2 0.7845128422758094 1101 3 0.8469848701821228 1101 4 0.9237687888073438 1101 5 0.8946651408062465 1101 6 1.0634911655997499 1101 7 0.8543042071662299 1101 8 0.7125977137258133 1101 9 0.9505500535647187 1101 10-11 1.0244960163223311 1101 12-13 0.8445095268913576 1101 14-15 1.043878705418912 1101 16-17 1.010391657338694 1101 18-19 0.8742897169787724 1101 20-21 0.9933757575219389 1101 22-23 0.8939171890483095 1101 24-25 1.044325693433528 1101 26-27 1.020286535607788 1101 28-29 1.1420142626639773 1101 30-31 1.1173964612235707 1101 32-33 1.1736814127119573 1101 34-35 1.034412083863053 1101 36-37 1.0669004212983495 1101 38-39 0.9980752570936389 1101 40-41 0.8653013581060449 1101 42-43 0.88925042885176 1101 44-45 0.8230662503506565 1101 46-47 0.9452718894647703 1101 48-49 0.6517483983069035 1101 50-51 0.6430600754354927 1101 52-53 0.8289843241433559 1101 54-55 0.750541996984861 1101 56-57 0.5255941250276699 1101 58-59 0.3221639840786885 1101 60-61 0.40032210489180287 1101 62-63 0.4227265227999517 1101 64-65 0.4931908344393463 1101 66-67 0.28636247514759816 1101 68-69 0.320285096005712 1101 70-71 0.44487911616643316 1101 72-73 0.2501413971319941 1101 74-75 0.032306785645221936 1101 76-77 -0.33224623033875744 1101 78-79 -0.30583389423116003 1101 80-81 -0.5268851324698112 1101 82-83 -0.6599258235608154 1101 84-85 -0.5346714648677846 1101 86-87 -0.6656197479918688 1101 88-89 -0.8285756110970688 1101 90-91 -1.133515822131276 1101 92-93 -0.9941635123059704 1101 94-95 -0.7056650439909724 1101 96-97 0.6414954637557724 1101 98-99 0.6165945328960163 1101 100-101 0.006040927637023685 1102 1 0.8193242346511305 1102 2 0.8375548215903521 1102 3 0.8621773374757531 1102 4 0.8834600380875699 1102 5 1.0059474633635617 1102 6 0.9224086037563524 1102 7 0.7511755294050957 1102 8 0.7717809076656366 1102 9 0.9754771397231821 1102 10-11 0.9075574040350176 1102 12-13 0.7249783500648022 1102 14-15 1.0324753145282344 1102 16-17 0.9877029304172211 1102 18-19 0.9557518868914414 1102 20-21 0.7950827884442404 1102 22-23 0.7168764159376124 1102 24-25 0.7661153871913271 1102 26-27 1.0887587615629144 1102 28-29 1.1400510864955038 1102 30-31 1.1212060414630045 1102 32-33 1.165401242752047 1102 34-35 1.0843771438596903 1102 36-37 0.8842527957118449 1102 38-39 0.9368336164283662 1102 40-41 1.042818524724261 1102 42-43 0.7858262270760932 1102 44-45 0.7159888431758148 1102 46-47 0.7978440926966783 1102 48-49 0.6757847383537658 1102 50-51 0.8849638291646684 1102 52-53 0.8525103080220866 1102 54-55 0.7188198946228717 1102 56-57 0.7000123821160535 1102 58-59 0.5414653021068361 1102 60-61 0.6813093473110854 1102 62-63 0.4824503705332077 1102 64-65 0.36892296596687757 1102 66-67 0.24917191611568512 1102 68-69 0.08085214619796233 1102 70-71 0.31870895183439174 1102 72-73 0.29135129859141173 1102 74-75 0.017517818378877337 1102 76-77 -0.10208248063297987 1102 78-79 -0.3477365077271237 1102 80-81 -0.348635997214533 1102 82-83 -0.5798452905180795 1102 84-85 -0.7928618618236882 1102 86-87 -0.7591377461658659 1102 88-89 -0.7711048234646931 1102 90-91 -0.8668171474894919 1102 92-93 -0.8180471832704974 1102 94-95 -0.5673529230676095 1102 96-97 0.7561477048434266 1102 98-99 0.703251381340289 1102 100-101 0.2098119028377674 1103 1 0.7111551317835918 1103 2 0.7194403102379319 1103 3 0.8012457532910382 1103 4 0.7224921784952016 1103 5 0.8812272597455362 1103 6 0.8300072114150225 1103 7 0.6619080944133984 1103 8 0.626593841533392 1103 9 0.9637633546718263 1103 10-11 0.7777487320320944 1103 12-13 0.7474486855033007 1103 14-15 0.9361920571155693 1103 16-17 1.0091218737126084 1103 18-19 0.9127986326107518 1103 20-21 0.8239200526045423 1103 22-23 0.9539838783638217 1103 24-25 0.970601247711393 1103 26-27 1.092310976345047 1103 28-29 1.0915838711385177 1103 30-31 0.9907881210151785 1103 32-33 1.040909395798593 1103 34-35 1.0034157860890147 1103 36-37 0.8752171466992067 1103 38-39 0.7620224269518019 1103 40-41 0.634450402680308 1103 42-43 0.6842721331089123 1103 44-45 0.8340081068048661 1103 46-47 0.5447097388945039 1103 48-49 0.4314124813097955 1103 50-51 0.7084818895602467 1103 52-53 0.8508041745048018 1103 54-55 0.7125127311218797 1103 56-57 0.6105883483700154 1103 58-59 0.5865460198445192 1103 60-61 0.656997192472577 1103 62-63 0.44812659963520574 1103 64-65 0.5081537290983782 1103 66-67 0.35642511449657377 1103 68-69 0.13573971225687131 1103 70-71 0.2573582181989593 1103 72-73 0.03591890762577421 1103 74-75 0.03084427326059469 1103 76-77 -0.06562103762501437 1103 78-79 -0.10093815934841288 1103 80-81 -0.23461238053656785 1103 82-83 -0.311373971877277 1103 84-85 -0.2939583504435177 1103 86-87 -0.47268879141299536 1103 88-89 -0.5502493383455374 1103 90-91 -0.7651016973374496 1103 92-93 -0.6901949054524117 1103 94-95 -0.47969270668381014 1103 96-97 0.835672120373264 1103 98-99 0.9383962054541204 1103 100-101 0.3452332526000923 1104 1 0.5226081118379007 1104 2 0.4986702586158529 1104 3 0.5974929819887862 1104 4 0.7741780200015995 1104 5 0.7442013549274833 1104 6 0.8753611185068095 1104 7 0.696792882993087 1104 8 0.697850183796831 1104 9 0.8962826901319403 1104 10-11 0.8534024153827175 1104 12-13 0.819051391305095 1104 14-15 0.9712403806326435 1104 16-17 1.0854675923352843 1104 18-19 1.0091771120279276 1104 20-21 0.8254988853801635 1104 22-23 0.7651193585079739 1104 24-25 0.9025515738300314 1104 26-27 1.150994076191104 1104 28-29 1.1000280197297059 1104 30-31 1.032969642489654 1104 32-33 1.102135800214917 1104 34-35 1.0807829735244638 1104 36-37 0.9460542745491267 1104 38-39 0.7968120155221143 1104 40-41 0.7769380669382784 1104 42-43 0.7228235056589867 1104 44-45 0.6626114050604954 1104 46-47 0.5776876639839656 1104 48-49 0.3875264492112116 1104 50-51 0.7998367332786884 1104 52-53 0.8120318280128949 1104 54-55 0.7245637186634362 1104 56-57 0.7181336505262728 1104 58-59 0.5229237229031192 1104 60-61 0.7139121548816441 1104 62-63 0.5360718840351417 1104 64-65 0.6503403622407014 1104 66-67 0.7335958294056013 1104 68-69 0.43111578592330346 1104 70-71 0.7564285864561953 1104 72-73 0.8075655814577409 1104 74-75 0.5695606988749695 1104 76-77 0.3356548615721273 1104 78-79 0.3679233088801688 1104 80-81 0.2682018232125891 1104 82-83 0.3681141157267156 1104 84-85 0.21192936850545507 1104 86-87 0.14778582118119488 1104 88-89 0.1383992039355455 1104 90-91 -0.08457382731371155 1104 92-93 -0.0487545758151775 1104 94-95 0.21140176866017413 1104 96-97 1.492019437833397 1104 98-99 1.7575468384265687 1104 100-101 1.5400898962597704 1105 1 0.6619341618380403 1105 2 0.7854193139219383 1105 3 0.7192381959358016 1105 4 0.7069285188832026 1105 5 0.768111202770811 1105 6 0.8958912355064186 1105 7 0.8135600704814578 1105 8 0.7869288611569516 1105 9 0.9062832997155112 1105 10-11 1.035407534902106 1105 12-13 0.6892420443293332 1105 14-15 0.9167721992131632 1105 16-17 0.9512263309500426 1105 18-19 0.9651813344428675 1105 20-21 0.7561352101286545 1105 22-23 0.6628728068594825 1105 24-25 0.805231198134571 1105 26-27 0.9801028215536078 1105 28-29 0.9467723805242656 1105 30-31 1.115567120023023 1105 32-33 1.0356588602926848 1105 34-35 1.1704772811590303 1105 36-37 1.044126010177152 1105 38-39 1.0284523920550441 1105 40-41 0.8836643775590076 1105 42-43 0.9627409915504188 1105 44-45 1.0567022500941121 1105 46-47 0.9297591729361017 1105 48-49 0.8809339899904849 1105 50-51 0.9679593523172656 1105 52-53 0.9028735548357822 1105 54-55 0.9725348125356383 1105 56-57 0.7320792635029889 1105 58-59 0.6779301480953315 1105 60-61 0.8961902453938073 1105 62-63 0.9105412430506483 1105 64-65 0.9139338774052881 1105 66-67 0.7731652102005881 1105 68-69 0.7352560752513924 1105 70-71 0.6806128867353252 1105 72-73 0.711296425634238 1105 74-75 0.6846715234729857 1105 76-77 0.6968184813980223 1105 78-79 0.4274990296466008 1105 80-81 0.1718250549147733 1105 82-83 0.3340390178277097 1105 84-85 0.22638583367584175 1105 86-87 0.250230478041388 1105 88-89 0.2936712873852336 1105 90-91 0.0919944530185397 1105 92-93 0.13255470009289283 1105 94-95 0.3927595126975838 1105 96-97 1.710786528045377 1105 98-99 1.9000514529137131 1105 100-101 1.3657609757748261 1106 1 0.9996526462492596 1106 2 1.0680253143910008 1106 3 1.0481611021837445 1106 4 0.9902867364852952 1106 5 1.0850930458964925 1106 6 1.047875671785107 1106 7 1.1477538117089239 1106 8 1.0711456755996736 1106 9 1.3281610858096968 1106 10-11 1.2150253207230577 1106 12-13 1.1800398452851937 1106 14-15 1.4461297701116749 1106 16-17 1.487272614548182 1106 18-19 1.4226571880799739 1106 20-21 1.4112423160293375 1106 22-23 1.1198348528081965 1106 24-25 1.313540862770374 1106 26-27 1.5539511066887073 1106 28-29 1.3591101864116908 1106 30-31 1.2758835835111455 1106 32-33 1.3005319501283026 1106 34-35 1.4794536575803576 1106 36-37 1.543984177128813 1106 38-39 1.4696834501579232 1106 40-41 1.5579600480269633 1106 42-43 1.5164051641995613 1106 44-45 1.6624497425429112 1106 46-47 1.691165217390143 1106 48-49 1.4785583759990537 1106 50-51 1.5990527746851342 1106 52-53 1.694934435682601 1106 54-55 1.5729918043261684 1106 56-57 1.5683846340522116 1106 58-59 1.4936302181082972 1106 60-61 1.7703281888504314 1106 62-63 1.5825318361238203 1106 64-65 1.4727081737214398 1106 66-67 1.4464719864029192 1106 68-69 1.5959898212135784 1106 70-71 1.7571889924592732 1106 72-73 1.718910409502712 1106 74-75 1.6800455810214139 1106 76-77 1.5947462661122636 1106 78-79 1.3371391335522524 1106 80-81 1.440464289554118 1106 82-83 1.4083159123953806 1106 84-85 1.3737649577645392 1106 86-87 1.471776317714884 1106 88-89 1.4089015740580706 1106 90-91 1.3369769785176828 1106 92-93 1.4865706187019434 1106 94-95 1.7985937270493864 1106 96-97 3.3533186233582857 1106 98-99 3.4791984671892653 1106 100-101 2.8370082770590486 1107 1 0.505770076304124 1107 2 0.4508538307218828 1107 3 0.5081634969962927 1107 4 0.10679866167068752 1107 5 0.5391942254263853 1107 6 0.48947067367971897 1107 7 0.5090646339093752 1107 8 0.5232747399990529 1107 9 0.5973170152540632 1107 10-11 0.6145681944279886 1107 12-13 0.6770341348905191 1107 14-15 0.7390155613909641 1107 16-17 0.6834613282986197 1107 18-19 0.6354334263385297 1107 20-21 0.7017940516045158 1107 22-23 0.5996454364533292 1107 24-25 0.6070538837460262 1107 26-27 0.7788687254128774 1107 28-29 0.7695137399068912 1107 30-31 0.9408299226493071 1107 32-33 1.015605128042587 1107 34-35 0.9904273403713972 1107 36-37 0.7208284162433216 1107 38-39 0.7652512699450895 1107 40-41 0.6930677438662229 1107 42-43 0.6750168734775173 1107 44-45 0.5008336625153404 1107 46-47 0.5041802592006661 1107 48-49 0.5319853909739614 1107 50-51 0.7687644874028265 1107 52-53 0.7329463322823315 1107 54-55 0.6124039452220025 1107 56-57 0.48794877895657507 1107 58-59 0.3735770988176732 1107 60-61 0.5812154999964321 1107 62-63 0.4883591621012684 1107 64-65 0.48172205429610315 1107 66-67 0.575115422021959 1107 68-69 0.30905114776085085 1107 70-71 0.3612709815737105 1107 72-73 0.2903735387078932 1107 74-75 0.3658686532317894 1107 76-77 0.2696876970273401 1107 78-79 0.3768708979983657 1107 80-81 0.2578439540652333 1107 82-83 0.2823469796311038 1107 84-85 0.229799036285943 1107 86-87 0.2770278800069832 1107 88-89 0.15129249848289916 1107 90-91 0.1815594558736393 1107 92-93 0.26548174495454546 1107 94-95 0.5638641933093034 1107 96-97 1.8763722205475855 1107 98-99 2.00098147014314 1107 100-101 1.4526508514045222 1108 1 0.5035420397235626 1108 2 0.5852201912401576 1108 3 0.5680506361177926 1108 4 0.5685748162664339 1108 5 0.7367594271721032 1108 6 0.7073546134761628 1108 7 0.5785105504230188 1108 8 0.6567097280053673 1108 9 0.5978327098636669 1108 10-11 0.5249425086558333 1108 12-13 0.410999086707875 1108 14-15 0.49211035273554415 1108 16-17 0.5011508856623195 1108 18-19 0.5865900254378502 1108 20-21 0.4112357270469076 1108 22-23 0.4044465407950213 1108 24-25 0.5794430167758122 1108 26-27 0.6340293380322137 1108 28-29 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2.9146729795860686 2311 96-97 4.341650289344111 2311 98-99 4.7072752279227945 2311 100-101 4.369711116918971 2312 1 -0.033805787803874665 2312 2 -0.4466930497436117 2312 3 -0.7151138518795861 2312 4 -0.3673211377035841 2312 5 -1.9689000819738922 2312 6 -0.4507762833797031 2312 7 0.02925986295500138 2312 8 0.3064047388465596 2312 9 0.2322732931163216 2312 10-11 0.119752447216257 2312 12-13 0.18890007290697497 2312 14-15 0.3434702877988016 2312 16-17 -0.3323126095097564 2312 18-19 0.10233709242502442 2312 20-21 0.3575537698153326 2312 22-23 0.29734730612086224 2312 24-25 0.3239869871198948 2312 26-27 0.47834093130147437 2312 28-29 0.4840916764807943 2312 30-31 0.2908696533371824 2312 32-33 -0.37039511201422215 2312 34-35 0.008276905631468878 2312 36-37 0.3785963952756646 2312 38-39 0.5797904959806317 2312 40-41 0.6957323560191639 2312 42-43 0.5976392312213434 2312 44-45 0.5180292659834151 2312 46-47 0.30302907102057475 2312 48-49 0.6412533298436607 2312 50-51 0.5798467667807898 2312 52-53 0.37763540633397596 2312 54-55 0.4779529262764477 2312 56-57 0.46619974272215003 2312 58-59 0.8773478103802326 2312 60-61 0.8990498457285945 2312 62-63 0.9680217003178164 2312 64-65 0.82195800109319 2312 66-67 0.8448086731722952 2312 68-69 0.7701986523740416 2312 70-71 0.9431707536300316 2312 72-73 1.14642761141128 2312 74-75 1.345442668203912 2312 76-77 1.0937081558961061 2312 78-79 1.1397091120272016 2312 80-81 1.1132128171536273 2312 82-83 1.5018202552981101 2312 84-85 1.5309705539320504 2312 86-87 1.51872080250374 2312 88-89 1.5226857598714894 2312 90-91 1.7120132165399475 2312 92-93 1.5931262711906768 2312 94-95 2.0800558226057184 2312 96-97 3.634858384838717 2312 98-99 3.954628982568286 2312 100-101 3.496566394788541 2313 1 -0.03531597251901175 2313 2 -0.4195717027998924 2313 3 -0.8116810534048433 2313 4 -0.07959813974571261 2313 5 -0.1609636261163061 2313 6 0.3184866660053487 2313 7 0.13697314058151022 2313 8 0.2776726147378312 2313 9 0.5040919036569704 2313 10-11 -0.5968653173382847 2313 12-13 0.13176341716246043 2313 14-15 0.23201616084031684 2313 16-17 -0.376013214650861 2313 18-19 0.05965117306050871 2313 20-21 -2.2338699126038506 2313 22-23 -0.2246398778962586 2313 24-25 0.30950939003195543 2313 26-27 0.3523367013709304 2313 28-29 0.22602026809996545 2313 30-31 0.36109486505004185 2313 32-33 0.5525465465281343 2313 34-35 0.18043599817940503 2313 36-37 -0.6449587149650142 2313 38-39 0.21402446825028676 2313 40-41 0.4104643366304188 2313 42-43 0.4332705011235092 2313 44-45 0.4772384146323745 2313 46-47 0.3592610256724811 2313 48-49 0.122441640352001 2313 50-51 0.4888877565816472 2313 52-53 0.37865934012611646 2313 54-55 0.3702614076261028 2313 56-57 0.5576243084800474 2313 58-59 0.5050547207570482 2313 60-61 0.3027487635124757 2313 62-63 0.3714169839380439 2313 64-65 0.3073524460824615 2313 66-67 0.48263258807180165 2313 68-69 0.2875448468787347 2313 70-71 0.6096249022213058 2313 72-73 0.551999531768022 2313 74-75 0.6357815746233655 2313 76-77 0.798494752078355 2313 78-79 0.6053548975280449 2313 80-81 0.7031841645300041 2313 82-83 0.733772978288858 2313 84-85 0.779378777563128 2313 86-87 0.7137954500335653 2313 88-89 0.9971007733126633 2313 90-91 1.2074130048270924 2313 92-93 1.395463260945391 2313 94-95 1.7356257599571734 2313 96-97 3.138156609363948 2313 98-99 3.3186528500198893 2313 100-101 2.8849086312146888 2314 1 -0.16892400731501667 2314 2 -0.12543888190134922 2314 3 -1.0599960728225675 2314 4 -0.11599870127744794 2314 5 -0.4497283595617958 2314 6 -0.5501020455954944 2314 7 -0.23140171718860358 2314 8 -1.7074186610729676 2314 9 -1.4209227701624982 2314 10-11 -0.7092782269836206 2314 12-13 -0.47646727214929285 2314 14-15 -0.4758910645542045 2314 16-17 -0.4907798616554828 2314 18-19 -1.7792317798878656 2314 20-21 -1.1871268170067495 2314 22-23 -1.745645854023742 2314 24-25 -1.5820194532580487 2314 26-27 -1.627907560278544 2314 28-29 -2.703603497495873 2314 30-31 -1.4521913259114712 2314 32-33 -1.138234939067381 2314 34-35 -1.3894571745243312 2314 36-37 -2.993646149812669 2314 38-39 -3.3173472742781343 2314 40-41 -3.9804322344659084 2314 42-43 -2.7592851859486416 2314 44-45 -2.9296550374411012 2314 46-47 -2.8779626258776716 2314 48-49 -1.2225071699779022 2314 50-51 -0.8625101537733855 2314 52-53 -2.227092706209998 2314 54-55 -1.4568478593926208 2314 56-57 -1.470097594136984 2314 58-59 -3.57595923289637 2314 60-61 -1.9583818157383668 2314 62-63 -1.7369303578755613 2314 64-65 -1.2124025651214154 2314 66-67 -2.498605283122018 2314 68-69 -2.1633840429777464 2314 70-71 -1.7822877654296256 2314 72-73 -1.1640561353803065 2314 74-75 -1.693688828471835 2314 76-77 -0.7015409326436561 2314 78-79 -0.42288880552736785 2314 80-81 -0.32572000583056493 2314 82-83 -0.24293566000978117 2314 84-85 -0.09712579793447418 2314 86-87 0.2947181467819959 2314 88-89 0.5500692259096773 2314 90-91 0.9576118794367616 2314 92-93 1.2419901612995403 2314 94-95 1.7956014703503662 2314 96-97 3.3065096563701974 2314 98-99 3.309285765011209 2314 100-101 2.924957832865836 2315 1 -2.042220702862899 2315 2 -1.6357759237512646 2315 3 -1.4026367324686184 2315 4 -1.4610708806183084 2315 5 -1.8199014291007067 2315 6 -2.76628623772136 2315 7 -1.9283732557978261 2315 8 -1.286293782398964 2315 9 -1.0996731508802213 2315 10-11 -3.3348583771019555 2315 12-13 -1.776434677823147 2315 14-15 -1.0962366607018694 2315 16-17 -1.8316402029859589 2315 18-19 -1.029155210441992 2315 20-21 -0.760402350870784 2315 22-23 -0.9323362733287652 2315 24-25 -2.5598688291772547 2315 26-27 -3.8875334414104685 2315 28-29 -5.652492457244676 2315 30-31 -8.432745556855735 2315 32-33 -6.6439938687498525 2315 34-35 -5.9098693296118405 2315 36-37 -3.5295583494900846 2315 38-39 -6.1603725217891 2315 40-41 -3.7498516902973194 2315 42-43 -3.6291934011261517 2315 44-45 -2.6318383351025147 2315 46-47 -1.9219268388556152 2315 48-49 -2.623722529849484 2315 50-51 -2.6134286538107148 2315 52-53 -1.735198290346542 2315 54-55 -3.037650864188773 2315 56-57 -3.095752665652661 2315 58-59 -1.7782759299019482 2315 60-61 -2.0796601518033846 2315 62-63 -2.765629604647014 2315 64-65 -2.047557084670455 2315 66-67 -2.4404235776606598 2315 68-69 -1.654268538403798 2315 70-71 -2.028764404323155 2315 72-73 -1.2204295982880318 2315 74-75 -1.9030915601289458 2315 76-77 -1.1315034252811387 2315 78-79 -1.1029948080089937 2315 80-81 -0.7780361287967636 2315 82-83 -0.5368092488358194 2315 84-85 -0.31374750065229406 2315 86-87 -0.8674872154716624 2315 88-89 -1.463057962106216 2315 90-91 -1.2190493682981938 2315 92-93 -0.3241696545219632 2315 94-95 0.19877291569115485 2315 96-97 1.7581094337466165 2315 98-99 1.600750851269698 2315 100-101 1.4990722049156435 2316 1 -3.585297481741545 2316 2 -2.1763052322351015 2316 3 -2.431450229308105 2316 4 -3.0457008963074514 2316 5 -2.5036102600329073 2316 6 -5.995007542952745 2316 7 -3.5285132371953303 2316 8 -4.681236467486642 2316 9 -6.483964548598731 2316 10-11 -5.7815107486217485 2316 12-13 -3.7574111850074345 2316 14-15 -5.087573044999836 2316 16-17 -4.560739744550244 2316 18-19 -2.6522573125360225 2316 20-21 -3.3556058947776854 2316 22-23 -4.080471337243509 2316 24-25 -2.978460146778467 2316 26-27 -4.0502053995799265 2316 28-29 -3.6745337812337766 2316 30-31 -3.452436047075693 2316 32-33 -3.519149648239825 2316 34-35 -5.538621656416389 2316 36-37 -5.67544586050472 2316 38-39 -5.6558274546147445 2316 40-41 -4.967760816863546 2316 42-43 -4.256010554884504 2316 44-45 -3.6028355895029947 2316 46-47 -3.8373886491125404 2316 48-49 -4.324565798742718 2316 50-51 -5.458100122142291 2316 52-53 -3.262765327391538 2316 54-55 -3.5283892418075133 2316 56-57 -2.667669880961629 2316 58-59 -3.1435922290558764 2316 60-61 -3.3391314701687556 2316 62-63 -2.4078444882560426 2316 64-65 -2.830140183980088 2316 66-67 -3.5005528190417827 2316 68-69 -2.9218702775222916 2316 70-71 -2.276961503921733 2316 72-73 -2.383643258645609 2316 74-75 -3.6285591075485932 2316 76-77 -3.7404628764955525 2316 78-79 -3.6204280814036025 2316 80-81 -3.9751233769526344 2316 82-83 -3.0095064958932127 2316 84-85 -2.830345012419148 2316 86-87 -2.481422618064073 2316 88-89 -2.5834190168008995 2316 90-91 -2.106368895427032 2316 92-93 -1.8618200834062186 2316 94-95 -1.7266880721993143 2316 96-97 0.12374079925104375 2316 98-99 0.5167696991327979 2316 100-101 0.258166219759433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41784.0 3 13568.0 4 13312.0 5 15373.0 6 17136.0 7 20121.0 8 24915.0 9 29892.0 10 34835.0 11 40184.0 12 46008.0 13 50862.0 14 54544.0 15 59007.0 16 65064.0 17 69777.0 18 68727.0 19 71593.0 20 78204.0 21 86906.0 22 96977.0 23 108990.0 24 121835.0 25 136846.0 26 154135.0 27 173118.0 28 195930.0 29 222026.0 30 249385.0 31 282820.0 32 323223.0 33 368428.0 34 418288.0 35 450935.0 36 433429.0 37 293305.0 38 61516.0 39 455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89253855238332 17.796092930743058 12.93489354108478 24.37647497578884 2 16.419301035288676 19.31370648261891 39.03830449801321 25.228687984079208 3 20.043633140733924 23.111006840738032 28.441649196366175 28.403710822161866 4 13.576787288609438 15.287666046028814 35.261742850564794 35.87380381479696 5 14.9090556868096 36.48503473268796 33.24046282442259 15.365446756079852 6 34.40344627666433 34.16099082258979 16.779942897629454 14.655620003116427 7 30.366122112370082 31.29221739814075 19.825370389980456 18.516290099508716 8 27.41503060077639 34.28662767330613 19.095241456932285 19.203100268985196 9 27.28824431045293 16.011201587252557 19.10397330617311 37.5965807961214 10-11 26.198898576785997 25.313008705268658 26.57900260198373 21.90909011596161 12-13 26.770281676482945 22.483807981975207 27.063942664507163 23.681967677034685 14-15 23.73279418720041 24.048868072867748 24.74650068217133 27.471837057760517 16-17 23.83429077196015 27.227916116805005 25.292583558166214 23.645209553068632 18-19 23.898364068887222 25.914615066773106 27.090084524444695 23.096936339894977 20-21 24.414532622798543 25.240494704446508 27.12575809667261 23.219214576082344 22-23 24.0662345350739 25.106822127010563 27.10994270577257 23.717000632142966 24-25 23.808917831568486 25.344763702432786 27.15839359564265 23.687924870356085 26-27 23.658331406383983 25.654299666316604 27.008935023267917 23.678433904031497 28-29 23.503332440427112 25.883989703041603 27.103815352480332 23.50886250405095 30-31 23.730329554790607 25.61916600945462 26.926437548061454 23.72406688769332 32-33 23.55270555933936 25.636286044232147 26.958820385000404 23.852188011428087 34-35 23.961730389985405 25.652648757281156 26.933539888759068 23.45208096397437 36-37 24.0631310927253 25.44906965708685 26.811552765245793 23.67624648494206 38-39 23.693459395515582 25.686172993354745 26.778671771763634 23.841695839366036 40-41 23.960135096835618 25.506876485114617 26.943090483077032 23.589897934972733 42-43 23.788831590986618 25.489345265565746 26.821427854806668 23.90039528864097 44-45 23.629828740318843 25.593476253575453 27.004645672064292 23.77204933404141 46-47 23.79182450849137 25.841793795630874 26.591245796111362 23.77513589976639 48-49 23.756710548159667 25.858025748350457 26.483557337786056 23.901706365703824 50-51 23.619571084573924 25.98655557307866 26.554083335851374 23.83979000649604 52-53 23.747489687902476 25.799366408823648 26.501806982917376 23.951336920356496 54-55 23.503047068161997 25.911384522953963 26.56591833829214 24.0196500705919 56-57 23.377466088445306 26.00164384986064 26.52415685447151 24.096733207222538 58-59 23.48480856764889 25.70398041632079 26.51401216422598 24.297198851804335 60-61 23.36655722479413 25.764414905407087 26.755703203916585 24.113324665882203 62-63 23.349271178679512 25.746399236203445 26.7438456225572 24.16048396255984 64-65 23.111970937131442 25.804762644278018 26.65127928548297 24.43198713310757 66-67 23.17177928942624 25.787794530438134 26.550697822421665 24.489728357713954 68-69 23.227176812952795 25.58216271090742 26.723839986002794 24.466820490136996 70-71 23.072321902081537 25.497759987254394 26.611837003080257 24.81808110758382 72-73 23.117150297372614 25.691783507035716 26.41351102824944 24.77755516734223 74-75 22.949854236594337 25.858617878042562 26.43781699889494 24.75371088646816 76-77 23.115567395908545 25.51562827538405 26.50582330655868 24.862981022148727 78-79 23.08821315066183 25.631031803553178 26.505684727026697 24.775070318758292 80-81 23.15216305575954 25.625425711920833 26.443003416738154 24.779407815581468 82-83 23.031838264000033 25.59493902917081 26.469279381446714 24.903943325382443 84-85 22.960313100562164 25.565285608509274 26.34737456691272 25.127026724015845 86-87 22.973203801647916 25.420298872953907 26.555423972059693 25.051073353338488 88-89 22.98893292239557 25.34832575713249 26.39985419330247 25.262887127169474 90-91 23.002795865487325 25.393287453853464 26.37461546436689 25.229301216292317 92-93 23.060973927909043 25.123432762743914 26.445435542251992 25.370157767095048 94-95 22.93419467986886 24.986112764552587 26.609950668444288 25.46974188713427 96-97 23.0968694293458 24.667240410254433 26.517015457757072 25.718874702642697 98-99 22.980774001563216 24.72341949621621 26.389330536074535 25.906475966146044 100-101 22.963350699090245 24.697033436917142 26.550371945422825 25.789243918569788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5917.0 1 3354.5 2 745.5 3 658.0 4 582.0 5 579.0 6 752.0 7 1166.0 8 1733.0 9 2012.0 10 1858.0 11 1716.0 12 1797.5 13 2006.5 14 2265.0 15 2511.0 16 2840.5 17 3188.0 18 3569.0 19 4123.0 20 4771.0 21 5622.5 22 6780.5 23 8221.5 24 10159.0 25 12910.0 26 16720.5 27 21515.5 28 26377.0 29 31933.0 30 38198.5 31 45961.0 32 55957.5 33 62035.5 34 66011.5 35 73221.5 36 82672.5 37 92674.0 38 102174.0 39 109854.5 40 117576.5 41 127686.5 42 136430.5 43 145700.0 44 155450.5 45 164389.5 46 173920.0 47 184690.0 48 194761.5 49 200283.0 50 202946.0 51 205195.5 52 207833.0 53 209571.5 54 208048.5 55 199939.5 56 187200.5 57 173451.5 58 159914.0 59 143721.0 60 123006.5 61 102976.0 62 84953.0 63 67263.0 64 51657.5 65 39274.0 66 29050.0 67 21678.0 68 16082.5 69 11664.5 70 8624.0 71 6260.5 72 4590.5 73 3581.5 74 2811.5 75 2095.5 76 1564.0 77 1101.5 78 735.0 79 511.5 80 351.0 81 218.0 82 152.0 83 102.0 84 64.0 85 47.5 86 29.5 87 24.5 88 22.0 89 17.5 90 13.5 91 8.0 92 5.0 93 3.5 94 5.0 95 4.0 96 3.0 97 2.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.1218195104670055 2 0.11539109309730161 3 0.12852829494940676 4 0.1438884074807553 5 0.1389819830085514 6 0.13958276967861719 7 0.1473329177224658 8 0.14629155416101844 9 0.15482272487595256 10-11 0.14918534328850197 12-13 0.1488248712864625 14-15 0.13622837743741653 16-17 0.13961280901212048 18-19 0.15846749734101834 20-21 0.19039930885501474 22-23 0.2083327909564784 24-25 0.22719749239654402 26-27 0.23000116352351768 28-29 0.23217400864692228 30-31 0.23130286797532687 32-33 0.23414659154697162 34-35 0.20277551425836993 36-37 0.19101010863624832 38-39 0.20422741537769556 40-41 0.1926622719789292 42-43 0.19531574643838642 44-45 0.20021215779942259 46-47 0.22053877346998157 48-49 0.23891283246282682 50-51 0.25488374477540887 52-53 0.27523038666830346 54-55 0.26875190374276076 56-57 0.23740085267649458 58-59 0.23129285486415915 60-61 0.20912382673873167 62-63 0.19880030912476798 64-65 0.1902290859651628 66-67 0.18563306793915954 68-69 0.1922817737545542 70-71 0.19577634955210352 72-73 0.23342564754289266 74-75 0.24561160383406033 76-77 0.26276406326443846 78-79 0.25158943120121485 80-81 0.25684631456429047 82-83 0.2537222238799484 84-85 0.23876263579531037 86-87 0.22835901329200456 88-89 0.2305919370824157 90-91 0.2329550313180078 92-93 0.24023456313697153 94-95 0.24552148583355046 96-97 8.643337586235416 98-99 0.3053197857274315 100-101 0.3172353880170696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4993453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.66664403709065 #Duplication Level Percentage of deduplicated Percentage of total 1 70.86105053457467 31.651253203221387 2 12.989146092519569 11.60363129720677 3 5.556571684588484 7.445802285062728 4 3.100511180169912 5.539577168706771 5 1.824511616237855 4.07474054520166 6 1.2274675808695135 3.28961145010604 7 0.8422603338734991 2.633465976378209 8 0.6179738643751713 2.2082254899416887 9 0.4447586759961782 1.787928971681612 >10 2.324608877499011 18.224001313773556 >50 0.1393282985028801 4.2917306355659415 >100 0.06761467441682037 5.253859850116848 >500 0.002919365335700889 0.8731383775312898 >1k 0.0011859909664769218 0.8287819273949542 >5k 9.123007434409439E-5 0.2942515081105616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8496 0.17014278496263008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5946 0.11907591800703841 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0026222335526137E-5 2 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 3 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 4 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 5 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 6 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 7 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 8 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 9 0.0 4.0052444671052274E-5 0.0 0.0 2.0026222335526137E-5 10-11 0.0 4.0052444671052274E-5 0.0 1.0013111167763069E-5 2.0026222335526137E-5 12-13 0.0 4.0052444671052274E-5 0.0 2.0026222335526137E-5 3.5045889087170746E-4 14-15 0.0 4.0052444671052274E-5 0.0 3.0039333503289206E-5 4.0052444671052277E-4 16-17 0.0 4.0052444671052274E-5 0.0 6.007866700657841E-5 4.806293360526273E-4 18-19 0.0 4.0052444671052274E-5 0.0 6.007866700657841E-5 5.006555583881534E-4 20-21 0.0 8.010488934210455E-5 0.0 1.7022288985197218E-4 5.507211142269688E-4 22-23 0.0 8.010488934210455E-5 0.0 3.4044577970394435E-4 5.80760447730258E-4 24-25 0.0 8.010488934210455E-5 0.0 8.411013380920979E-4 5.80760447730258E-4 26-27 0.0 8.010488934210455E-5 0.0 0.0016922157873519588 5.907735588980211E-4 28-29 0.0 8.010488934210455E-5 0.0 0.006989151595098622 6.408391147368364E-4 30-31 0.0 1.0013111167763069E-4 0.0 0.027345806599160943 6.608653370723626E-4 32-33 0.0 1.0013111167763069E-4 0.0 0.07479794042319013 6.909046705756518E-4 34-35 0.0 1.0013111167763069E-4 0.0 0.1460011739371533 7.710095599177563E-4 36-37 0.0 1.0013111167763069E-4 0.0 0.2628641943761161 7.810226710855193E-4 38-39 0.0 1.0013111167763069E-4 0.0 0.4437710738440914 8.411013380920979E-4 40-41 0.0 1.0013111167763069E-4 0.0 0.646606666769468 8.61127560427624E-4 42-43 0.0 1.0013111167763069E-4 0.0 0.8498928496973939 8.61127560427624E-4 44-45 0.0 1.0013111167763069E-4 0.0 1.093481805075566 8.71140671595387E-4 46-47 0.0 1.2015733401315682E-4 0.0 1.3767427069004154 9.111931162664392E-4 48-49 0.0 1.2015733401315682E-4 0.0 1.6797895163927645 0.001001311116776307 50-51 0.0 1.2015733401315682E-4 0.0 2.003463334890706 0.001001311116776307 52-53 0.0 1.2015733401315682E-4 0.0 2.312467945527874 0.001041363561447359 54-55 0.0 1.2015733401315682E-4 2.0026222335526137E-5 2.646425229195108 0.0011014422284539376 56-57 0.0 1.2015733401315682E-4 3.0039333503289206E-5 3.020815455757769 0.0011114553396217007 58-59 0.0 1.4018355634868297E-4 4.0052444671052274E-5 3.426116156495315 0.0011214684507894637 60-61 0.0 1.4018355634868297E-4 4.0052444671052274E-5 3.8517935384592583 0.0011414946731249898 62-63 1.0013111167763069E-5 1.4018355634868297E-4 4.0052444671052274E-5 4.269270182376804 0.0012015733401315684 64-65 2.0026222335526137E-5 1.4018355634868297E-4 4.0052444671052274E-5 4.699032913697195 0.0012115864512993314 66-67 2.0026222335526137E-5 1.5019666751644605E-4 4.0052444671052274E-5 5.170680489032339 0.0012616520071381467 68-69 2.0026222335526137E-5 1.602097786842091E-4 4.0052444671052274E-5 5.682831099041085 0.0013017044518091989 70-71 2.0026222335526137E-5 1.7022288985197218E-4 4.0052444671052274E-5 6.222627908984023 0.001321730674144725 72-73 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 6.765238403165105 0.0013617831188157774 74-75 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 7.296363858836761 0.001441888008157882 76-77 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 7.857879106902578 0.0015219928974999864 78-79 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 8.46775768190869 0.001602097786842091 80-81 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 9.095820066795461 0.0016521633426809063 82-83 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 9.730821537721493 0.0018123731213651154 84-85 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 10.359474696167162 0.0018323993437006417 86-87 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 10.917465329101926 0.0019125042330427463 88-89 2.0026222335526137E-5 1.8023600101973525E-4 4.0052444671052274E-5 11.475175594923993 0.0019825960112170877 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6150 0.0 48.348083 1 GTATCAA 11520 0.0 44.311306 1 TATCAAC 14800 0.0 34.173763 2 TCAACGC 14910 0.0 33.89179 4 ATCAACG 15030 0.0 33.55825 3 CAACGCA 15180 0.0 33.102 5 AACGCAG 15935 0.0 31.770153 6 ACGCAGA 18455 0.0 27.07507 7 GTACATG 18245 0.0 26.714878 1 TACATGG 18020 0.0 26.213497 2 CGCAGAG 19070 0.0 26.00479 8 ACATGGG 18770 0.0 24.114277 3 GCAGAGT 20525 0.0 22.785925 9 CATGGGG 12365 0.0 20.662054 4 AGAGTAC 17735 0.0 20.21512 10-11 GAGTACT 10035 0.0 19.129616 12-13 GAGTACA 13755 0.0 18.983212 1 CAGAGTA 19165 0.0 18.964537 10-11 AGTACTT 10760 0.0 18.496584 12-13 TGGTATC 3300 0.0 18.106556 2 >>END_MODULE