FastQCFastQC Report
Fri 27 May 2016
SRR939864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4993453
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.1878059130625641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72590.14537034793358422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50460.1010523179050649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.052.127331
GTATCAA117000.042.3116571
TATCAAC149100.032.7156262
TCAACGC150800.032.244994
ATCAACG151350.032.1905023
CAACGCA152850.031.781495
AACGCAG159950.030.4004066
ACGCAGA190850.025.4037887
GTACATG181350.024.889981
CGCAGAG193150.024.8556758
TACATGG178700.024.6412752
ACATGGG184050.022.7596023
GCAGAGT222200.021.0723429
GGACCGA13100.019.9173326
TGGTATC35100.019.3316382
GAGTACT109450.019.15782712-13
AGAGTAC190600.019.07783110-11
GTGGTAT38350.018.9359721
CAGAGTA203500.018.83591310-11
CATGGGG121400.018.4834164
Produced by FastQC (version 0.11.5)