##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4993453 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393381293465662 31.0 31.0 34.0 30.0 34.0 2 31.625771385051586 31.0 31.0 34.0 30.0 34.0 3 31.67851484734111 31.0 31.0 34.0 30.0 34.0 4 35.49098109064008 37.0 35.0 37.0 33.0 37.0 5 35.21470813883699 37.0 35.0 37.0 32.0 37.0 6 35.23082163785261 37.0 35.0 37.0 32.0 37.0 7 35.13000843304223 37.0 35.0 37.0 32.0 37.0 8 35.090040699291656 37.0 35.0 37.0 32.0 37.0 9 36.67757902197137 39.0 35.0 39.0 32.0 39.0 10-11 36.539061647321006 38.0 35.0 39.0 32.0 39.0 12-13 36.455124840466105 38.0 35.0 39.0 32.0 39.0 14-15 37.512227310440295 39.0 36.0 41.0 32.0 41.0 16-17 37.424644028891436 39.0 36.0 41.0 32.0 41.0 18-19 37.36597811173951 39.0 36.0 41.0 32.0 41.0 20-21 37.25917766723748 39.0 36.0 40.0 31.0 41.0 22-23 37.13138303294333 39.0 36.0 40.0 31.0 41.0 24-25 36.97745668177912 39.0 36.0 40.0 31.0 41.0 26-27 36.673296414324916 38.5 35.5 40.0 30.0 41.0 28-29 36.44431558682939 38.0 35.0 40.0 30.0 41.0 30-31 36.187217742912566 38.0 35.0 40.0 30.0 41.0 32-33 36.19050594848895 38.0 35.0 40.0 30.0 41.0 34-35 36.05328507147259 38.0 35.0 40.0 29.5 41.0 36-37 36.140301110273796 38.0 35.0 40.0 30.0 41.0 38-39 36.056434795721515 38.0 35.0 40.0 29.0 41.0 40-41 35.969429470949265 38.0 35.0 40.0 29.0 41.0 42-43 35.74857999063974 38.0 34.0 40.0 28.5 41.0 44-45 35.519213357970926 38.0 34.0 40.0 27.5 41.0 46-47 35.26853061398596 38.0 34.0 40.0 27.0 41.0 48-49 34.98236821293802 38.0 33.5 40.0 26.0 41.0 50-51 34.750932671239724 38.0 33.0 40.0 26.0 41.0 52-53 34.47727995036701 37.5 33.0 40.0 25.5 41.0 54-55 34.190821862146294 37.0 33.0 40.0 24.5 41.0 56-57 33.7984615054953 37.0 32.5 39.5 23.5 41.0 58-59 33.405410644698165 36.0 32.0 39.0 23.0 41.0 60-61 33.06533074407629 36.0 31.0 39.0 23.0 40.0 62-63 32.746757003620544 35.0 31.0 39.0 22.0 40.0 64-65 32.28447589273395 35.0 31.0 38.0 21.5 40.0 66-67 31.367980233317503 34.5 29.5 37.5 16.0 40.0 68-69 29.48119467630916 34.0 26.5 37.0 9.5 39.0 70-71 28.82442640393331 33.0 25.5 36.0 9.0 39.0 72-73 29.39510485029097 33.0 27.0 36.0 10.0 39.0 74-75 29.493974610354798 33.0 28.0 35.0 11.0 38.0 76-77 29.048736715855743 32.5 27.5 35.0 11.5 37.0 78-79 29.6035049293545 34.0 29.0 35.0 11.5 37.0 80-81 29.440831424667458 34.0 29.0 35.0 7.5 36.5 82-83 29.02364746398935 33.5 28.5 35.0 7.0 36.0 84-85 28.68835042604787 33.0 28.0 35.0 4.0 36.0 86-87 28.322228826425324 33.0 27.0 35.0 2.0 35.0 88-89 27.89141982511901 33.0 26.5 35.0 2.0 35.0 90-91 27.419196596022832 33.0 26.0 35.0 2.0 35.0 92-93 26.933109713859327 32.0 25.0 34.0 2.0 35.0 94-95 26.122791583299172 32.0 24.0 34.0 2.0 35.0 96-97 25.593561909964908 32.0 23.0 34.0 2.0 35.0 98-99 24.920760343593905 31.5 19.0 34.0 2.0 35.0 100-101 23.420327276535897 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2837656849161512 1101 2 0.35256172823529397 1101 3 0.354975851547465 1101 4 0.2109276320409066 1101 5 0.22866373597776857 1101 6 0.22293181106392268 1101 7 0.20955350648304716 1101 8 0.1679589484863584 1101 9 0.40867152662467987 1101 10-11 0.36412650960034654 1101 12-13 0.35355445135677144 1101 14-15 0.38942585106158134 1101 16-17 0.3312943396737822 1101 18-19 0.23652449276958265 1101 20-21 0.3663963122544729 1101 22-23 0.34973630896976715 1101 24-25 0.3292803981303578 1101 26-27 0.24204860019673902 1101 28-29 0.26036358163659656 1101 30-31 0.139282110560643 1101 32-33 0.09635350651364405 1101 34-35 0.12263931201122347 1101 36-37 0.2968069933081878 1101 38-39 0.3764424831234905 1101 40-41 0.28035620583293763 1101 42-43 0.11869913574094682 1101 44-45 0.012522985594721092 1101 46-47 0.03444096071778091 1101 48-49 0.162787852969565 1101 50-51 0.14725598510070625 1101 52-53 -0.10964139860380584 1101 54-55 -0.14775111321775114 1101 56-57 -0.2526510875733905 1101 58-59 -0.16469632399785894 1101 60-61 -0.18049533090692904 1101 62-63 -0.1783796877106525 1101 64-65 -0.2608401964557885 1101 66-67 -0.38018544864611314 1101 68-69 -9.19532261726253 1101 70-71 -6.951161029325878 1101 72-73 -2.0432658239047683 1101 74-75 -0.8873129840769245 1101 76-77 -0.6050679479471555 1101 78-79 -0.9287866920981962 1101 80-81 -0.8883018674859215 1101 82-83 -0.7799291274251594 1101 84-85 -0.6053119204807835 1101 86-87 -0.591484284522636 1101 88-89 -0.7247094248621728 1101 90-91 -1.0715081192535365 1101 92-93 -1.3467486831076592 1101 94-95 -1.082249369860623 1101 96-97 -1.5621643619562917 1101 98-99 -1.317450458556685 1101 100-101 -1.1667716544150224 1102 1 0.1334768394104202 1102 2 0.10273532874155933 1102 3 0.19454858173000744 1102 4 0.2192765824969598 1102 5 0.3116032816274128 1102 6 0.20309330066459097 1102 7 0.3330010993285697 1102 8 0.19388096956601686 1102 9 0.350584585471303 1102 10-11 0.28346478340129266 1102 12-13 0.23686644604002538 1102 14-15 0.38904214222501565 1102 16-17 0.365614178347073 1102 18-19 0.2678667142207871 1102 20-21 0.2908579796500774 1102 22-23 0.32474465710576794 1102 24-25 0.22024087357502964 1102 26-27 0.231209156435078 1102 28-29 0.13146852462900682 1102 30-31 0.10791515012103758 1102 32-33 0.015050500940347433 1102 34-35 -0.12522492937940655 1102 36-37 -0.0675352672367282 1102 38-39 0.15370403388952525 1102 40-41 0.1047117093721468 1102 42-43 0.019916334692908322 1102 44-45 0.09419391999140458 1102 46-47 0.057712163557930296 1102 48-49 -0.02532136752628844 1102 50-51 -0.04620825922195593 1102 52-53 0.07811799427087607 1102 54-55 -0.21747718847802844 1102 56-57 -0.20579230789984138 1102 58-59 -0.3539621481950732 1102 60-61 -0.02972200845467654 1102 62-63 -0.0020065618671836205 1102 64-65 -0.08474984844846745 1102 66-67 0.0840919000510496 1102 68-69 -4.75465905040129 1102 70-71 0.01225149320391239 1102 72-73 0.40378323218574863 1102 74-75 0.2067244717695793 1102 76-77 -0.034393992746917235 1102 78-79 -0.30755829849946537 1102 80-81 -0.2929371394600473 1102 82-83 -0.26921128139085226 1102 84-85 -0.39047274223057116 1102 86-87 -0.3342967226751661 1102 88-89 -0.39938751259334637 1102 90-91 -0.5376443331417384 1102 92-93 -0.6071033241418782 1102 94-95 -0.2867110413284877 1102 96-97 -0.7172081224779987 1102 98-99 -0.5859489709595707 1102 100-101 -0.6551226247127637 1103 1 0.16521801933247815 1103 2 0.2142328489309051 1103 3 0.16005542648315796 1103 4 0.14041741433368315 1103 5 0.2774185664587421 1103 6 0.13382233724526316 1103 7 0.1056795723519599 1103 8 0.1983043888106124 1103 9 0.31357078495829427 1103 10-11 0.3774807287225528 1103 12-13 0.289155959473824 1103 14-15 0.24740857934565952 1103 16-17 0.22393214914438175 1103 18-19 0.20359055727315933 1103 20-21 0.288429311373811 1103 22-23 0.21567530962700232 1103 24-25 0.1305767878144195 1103 26-27 0.21085260684084517 1103 28-29 0.09648079483520178 1103 30-31 0.1986458914476401 1103 32-33 0.20368827018118196 1103 34-35 0.10995617014207681 1103 36-37 0.11673012602437183 1103 38-39 0.22097449777864142 1103 40-41 0.2784298791886286 1103 42-43 0.15928684734569032 1103 44-45 0.1916218350078438 1103 46-47 0.09064636829395312 1103 48-49 0.04523221898163854 1103 50-51 -0.040541663916748405 1103 52-53 -0.06205553300704025 1103 54-55 -0.19239363610466853 1103 56-57 -0.026931157473853773 1103 58-59 -0.13398282319517563 1103 60-61 -0.27069597341620266 1103 62-63 -0.2501494790442891 1103 64-65 -0.27027625813459366 1103 66-67 -0.12566539170234137 1103 68-69 -4.556145747436222 1103 70-71 0.10383401281839966 1103 72-73 0.43315610373116087 1103 74-75 0.1696528882984829 1103 76-77 -0.02410357143680031 1103 78-79 -0.1825182567926582 1103 80-81 -0.1505063105174287 1103 82-83 -0.2912866509837322 1103 84-85 -0.2698888271031308 1103 86-87 -0.31646200770472177 1103 88-89 -0.47060535533648107 1103 90-91 -0.6352993163519756 1103 92-93 -0.565995853786621 1103 94-95 -0.5575112213179416 1103 96-97 -1.0107442611095223 1103 98-99 -0.6765834734314531 1103 100-101 -0.7874069446182475 1104 1 0.17853345286052047 1104 2 0.15899243816035025 1104 3 0.1295320820149719 1104 4 0.10897827421614181 1104 5 0.20115232850599085 1104 6 0.18753462836589563 1104 7 0.12459331987515299 1104 8 0.08926171387867754 1104 9 0.13984460393722742 1104 10-11 0.17640973822545192 1104 12-13 0.17716550484092863 1104 14-15 0.22613814156206047 1104 16-17 0.2486547306673188 1104 18-19 0.17583377811585166 1104 20-21 0.2687741606286167 1104 22-23 0.2745517304448697 1104 24-25 0.17106859559802245 1104 26-27 0.21380226978784123 1104 28-29 0.12816396905026295 1104 30-31 0.1448033296641995 1104 32-33 0.23184262563925984 1104 34-35 0.14497440015598073 1104 36-37 0.14836039639893528 1104 38-39 0.275724918674527 1104 40-41 0.09757789867676792 1104 42-43 0.13718924177774028 1104 44-45 0.11647899175598297 1104 46-47 0.012828892885210053 1104 48-49 0.05988300847489114 1104 50-51 0.008307663139866861 1104 52-53 -7.451319736375694E-4 1104 54-55 0.04430656323116011 1104 56-57 -0.009145647284242386 1104 58-59 -0.022421287457980554 1104 60-61 0.0219568111648627 1104 62-63 0.23657171464160598 1104 64-65 0.08545127920796602 1104 66-67 0.28752478674675075 1104 68-69 -4.125092077037717 1104 70-71 0.47456842689155465 1104 72-73 0.678072309851288 1104 74-75 0.34879180671184074 1104 76-77 0.2143652841895225 1104 78-79 -0.10618998702112137 1104 80-81 -0.05948385972635606 1104 82-83 -0.09286996127292468 1104 84-85 -0.07578934443850471 1104 86-87 -0.03954983557449765 1104 88-89 -0.18272016185888873 1104 90-91 -0.12968874204071668 1104 92-93 -0.28887371893889835 1104 94-95 -0.1600627506770742 1104 96-97 -0.5599646068542796 1104 98-99 -0.31405076884607297 1104 100-101 -0.22129801343917066 1105 1 0.1165880846242402 1105 2 0.174126776887082 1105 3 0.1558581180698937 1105 4 0.14927964658791382 1105 5 0.1800128546896076 1105 6 0.15821653091816046 1105 7 0.1442528623503705 1105 8 0.174762328431882 1105 9 0.18382494352553636 1105 10-11 0.12806976126264402 1105 12-13 0.11562609734179574 1105 14-15 0.10382293107640805 1105 16-17 0.21087910526500053 1105 18-19 0.14143314067207768 1105 20-21 0.18298513708587194 1105 22-23 0.16422505978813007 1105 24-25 0.16782293156821737 1105 26-27 0.2257502211014284 1105 28-29 0.16680170358829116 1105 30-31 0.22756240542062045 1105 32-33 0.21601890571845672 1105 34-35 0.1429660055893507 1105 36-37 0.008379223574515038 1105 38-39 0.10502007265144186 1105 40-41 0.09571953800759303 1105 42-43 0.07292464048450853 1105 44-45 0.002333467114084442 1105 46-47 0.08048286196015653 1105 48-49 0.01578662881551196 1105 50-51 0.05206374551218573 1105 52-53 0.06520355257865873 1105 54-55 0.12946912911616693 1105 56-57 0.0708069253757202 1105 58-59 0.06378858683590494 1105 60-61 0.0542213362695918 1105 62-63 0.0915793718502087 1105 64-65 0.12771556357820657 1105 66-67 0.7145921187476212 1105 68-69 2.0255340574991543 1105 70-71 2.3282963365259484 1105 72-73 1.1891219662717702 1105 74-75 0.7080417385068891 1105 76-77 0.48001711703875927 1105 78-79 0.4159643886381339 1105 80-81 0.45957231595041037 1105 82-83 0.32787824152317313 1105 84-85 0.2624502485093281 1105 86-87 0.24882686076751526 1105 88-89 0.2970921158797779 1105 90-91 0.24305399891683876 1105 92-93 0.20450332376600144 1105 94-95 0.5388497340970346 1105 96-97 0.07916367806137714 1105 98-99 0.3086416805223031 1105 100-101 0.34054484423115383 1106 1 0.3888153705782891 1106 2 0.37000240963866204 1106 3 0.32475885702302776 1106 4 0.22917950792814423 1106 5 0.310188781607458 1106 6 0.37705876300064745 1106 7 0.32819069975013093 1106 8 0.3480441239071723 1106 9 0.4236786245764179 1106 10-11 0.3403990709469227 1106 12-13 0.42767734732107954 1106 14-15 0.5616018802645826 1106 16-17 0.5032675702558151 1106 18-19 0.5443727994011027 1106 20-21 0.5125127186629115 1106 22-23 0.5279962968018452 1106 24-25 0.5650337950072597 1106 26-27 0.6245267993449843 1106 28-29 0.6241843076556535 1106 30-31 0.6082539215523255 1106 32-33 0.7065745570105051 1106 34-35 0.610065797511119 1106 36-37 0.7536108870417166 1106 38-39 0.8510926555023133 1106 40-41 0.7666672989175041 1106 42-43 0.6598672557977281 1106 44-45 0.834871393625555 1106 46-47 0.9336500460959982 1106 48-49 0.8912404994915661 1106 50-51 0.9364468186030379 1106 52-53 0.8919809903639546 1106 54-55 0.9152178585932376 1106 56-57 0.8757204395002276 1106 58-59 1.0910327370445216 1106 60-61 0.9494985851344424 1106 62-63 0.938026720725766 1106 64-65 0.8682411257912221 1106 66-67 1.4015018358803566 1106 68-69 3.0275530671828186 1106 70-71 3.2841867703090877 1106 72-73 2.2738098199808547 1106 74-75 1.6104562310924102 1106 76-77 1.2393855097693312 1106 78-79 1.155374735604095 1106 80-81 1.2672446747975918 1106 82-83 1.1248569200297212 1106 84-85 1.1907740661345088 1106 86-87 1.1772238882617856 1106 88-89 1.3777038787273597 1106 90-91 1.3393618868304245 1106 92-93 1.3295847178252167 1106 94-95 1.6485354608025276 1106 96-97 1.4665316429108728 1106 98-99 1.6012272944345938 1106 100-101 1.8845363966902298 1107 1 0.016153065979800374 1107 2 0.026898427610706932 1107 3 0.04906987264459062 1107 4 0.022713372204847815 1107 5 -0.005221624369220024 1107 6 0.09013843086481188 1107 7 0.0926005228630018 1107 8 0.18565818138590373 1107 9 0.15574051129232913 1107 10-11 0.10167837996745988 1107 12-13 0.005138875177600255 1107 14-15 0.0889645245852364 1107 16-17 0.09791751253090553 1107 18-19 0.0589699068631262 1107 20-21 0.0763736970758302 1107 22-23 0.053911040923530607 1107 24-25 0.1392860849236328 1107 26-27 0.0713120393090989 1107 28-29 0.08577075394650535 1107 30-31 0.10218377308773796 1107 32-33 0.06615554282929281 1107 34-35 0.13467086128275696 1107 36-37 0.045409576057181766 1107 38-39 0.10903965654334513 1107 40-41 0.009805213366753662 1107 42-43 -0.060488595695048275 1107 44-45 -0.05311544570140114 1107 46-47 -0.03736878194546733 1107 48-49 0.20026430526005612 1107 50-51 0.15876806730037885 1107 52-53 0.25224574678082234 1107 54-55 0.1606118141226176 1107 56-57 0.19291875941195258 1107 58-59 0.1845185474649682 1107 60-61 0.33321920657056836 1107 62-63 0.2969181636523359 1107 64-65 0.13416250194397605 1107 66-67 0.6007063664653742 1107 68-69 2.0685262885843407 1107 70-71 2.3541171879627427 1107 72-73 1.4714632976974542 1107 74-75 0.7224625162342093 1107 76-77 0.3866810008353383 1107 78-79 0.27968211448333946 1107 80-81 0.34020227656816004 1107 82-83 0.42724910844906105 1107 84-85 0.28835789018836167 1107 86-87 0.24723696989577704 1107 88-89 0.4167293153167009 1107 90-91 0.28164627401196185 1107 92-93 0.3501372370927598 1107 94-95 0.6922642926868043 1107 96-97 0.3528486540744922 1107 98-99 0.568778175434435 1107 100-101 0.6149939402453413 1108 1 -0.19106412600767442 1108 2 -0.13407494738239478 1108 3 -0.11666280076495283 1108 4 -0.10071075986861189 1108 5 -0.10745895955521689 1108 6 -0.053961377190802295 1108 7 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-0.6765773711946466 2316 52-53 -0.5547750542785721 2316 54-55 -0.5765026791373202 2316 56-57 -0.42135907323105926 2316 58-59 -0.4653709900214338 2316 60-61 -0.5075627833188392 2316 62-63 -0.4480360083729309 2316 64-65 -0.3276766884497633 2316 66-67 0.18341028084064348 2316 68-69 1.390930458730974 2316 70-71 1.666224246964049 2316 72-73 0.776071578187981 2316 74-75 0.2242417149059115 2316 76-77 0.015145456862359907 2316 78-79 -0.17721188400642873 2316 80-81 -0.3405873712985894 2316 82-83 -0.24000692069547114 2316 84-85 -0.27110310682620664 2316 86-87 -0.24560597351937474 2316 88-89 -0.3001496152137477 2316 90-91 -0.36365286554123344 2316 92-93 -0.37524799046576973 2316 94-95 -0.08811400033869532 2316 96-97 -0.07046555821865397 2316 98-99 -0.24821383201857117 2316 100-101 -0.47402476151834705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 25.0 4 118.0 5 213.0 6 543.0 7 1267.0 8 2927.0 9 5044.0 10 7731.0 11 11292.0 12 15835.0 13 20211.0 14 24557.0 15 29608.0 16 36015.0 17 41722.0 18 39776.0 19 37325.0 20 38368.0 21 40471.0 22 43879.0 23 50544.0 24 60688.0 25 75015.0 26 92949.0 27 115207.0 28 142056.0 29 171568.0 30 204139.0 31 243477.0 32 290727.0 33 353286.0 34 438698.0 35 555397.0 36 690844.0 37 719023.0 38 367217.0 39 25676.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00497408396491 18.60157571416324 13.768769995889652 24.6246802059822 2 16.579868118555087 20.08432736516946 38.06211315988046 25.27369135639499 3 19.86453857262242 23.393040204059275 29.036191603762607 27.706229619555696 4 14.013168843283394 15.955932698275122 34.998587150014224 35.03231130842725 5 14.675796487921284 36.731536273596646 33.468603789802366 15.124063448679701 6 34.42675215997914 34.70289385036521 16.38556180885724 14.484792180798408 7 30.133657010489536 31.42839233692597 19.814765453885315 18.623185198699176 8 27.213212981077422 34.753566319739065 18.908458735868745 19.124761963314764 9 26.76013972695848 15.943436335537752 19.044496864193974 38.251927073309794 10-11 26.032086413950427 25.507829952539858 26.648323314548065 21.81176031896165 12-13 26.485209733625208 22.52840869834962 27.229354116279858 23.757027451745316 14-15 23.307538891424432 24.191456292869884 24.82379427622529 27.677210539480395 16-17 23.543711120846112 27.373868202015146 25.4594355091057 23.622985168033043 18-19 23.603378624308533 26.03183869276818 27.25921594327772 23.105566739645568 20-21 24.143964106601185 25.32060479992502 27.244293678142164 23.291137415331637 22-23 23.829822769934953 25.208327784400897 27.252264114631696 23.709585331032454 24-25 23.707816723033865 25.47594367659381 27.181618793266697 23.63462080710563 26-27 23.50359201200134 25.817968010436086 27.03484430876424 23.64359566879833 28-29 23.452234772775814 25.953701944918038 27.083472056741538 23.51059122556461 30-31 23.6203788333324 25.702732547288083 26.967813345359787 23.709075274019735 32-33 23.458788339036925 25.611273727586166 27.036851456659107 23.893086476717805 34-35 23.826414721005136 25.713513814460576 26.98452069054879 23.4755507739855 36-37 23.942468642312882 25.526370125951956 26.905316523701938 23.625844708033224 38-39 23.585608839483093 25.690033381785216 26.903271398914992 23.821086379816702 40-41 23.851470250388207 25.545803468653062 26.97914774752452 23.62357853343421 42-43 23.692976091207584 25.519451184423776 26.904661025231295 23.882911699137345 44-45 23.500284799028506 25.61360083494275 27.075883230293517 23.810231135735226 46-47 23.71806551511598 25.806721888984104 26.67289045235196 23.802322143547958 48-49 23.62789861394506 25.86854562199086 26.56973626199833 23.93381950206575 50-51 23.545236270477037 25.984215194035816 26.62095729699755 23.849591238489598 52-53 23.74444162651959 25.72651274383971 26.57444871185819 23.954596917782517 54-55 23.43934476686355 25.861301986106312 26.632612412302812 24.066740834727323 56-57 23.342748129527937 25.930698579346718 26.58675608819926 24.139797202926086 58-59 23.352578000991443 25.692442503843093 26.557431062195498 24.39754843296997 60-61 23.28093733971114 25.800195035429034 26.735155386436727 24.1837122384231 62-63 23.234455356713717 25.801043786148004 26.69564181232677 24.26885904481151 64-65 23.007994587829163 25.77994971351484 26.592266007075033 24.619789691580966 66-67 22.844326521640067 25.80593693942321 26.78502090680667 24.564715632130056 68-69 22.617517338302278 25.57238089559838 27.226757414984032 24.583344351115315 70-71 22.584158623855803 25.57289666141967 26.933363195549298 24.90958151917523 72-73 22.839847230322338 25.759178910128743 26.543270063157827 24.85770379639109 74-75 22.966657515237827 25.88587650739408 26.155030679989892 24.9924352973782 76-77 22.93454330558811 25.652553662469824 26.307401063733888 25.105501968208177 78-79 23.045536416225374 25.719073388008585 26.168606596257955 25.066783599508092 80-81 23.06838794305469 25.767157065218754 26.126241954985467 25.03821303674109 82-83 22.931760606394377 25.774744915839747 26.06966125624568 25.22383322152019 84-85 22.875399975247497 25.672593249455183 25.93319547229422 25.5188113030031 86-87 22.797799935254517 25.570320494148426 26.176821257105757 25.455058313491303 88-89 22.792059506837266 25.554321937789215 25.978688452035996 25.674930103337516 90-91 22.76025974156015 25.50902436704266 25.990988149416484 25.739727741980705 92-93 22.696452460487208 25.29550479440533 26.088324000865153 25.91971874424231 94-95 22.405924139650512 25.118809880941594 26.453647311024547 26.021618668383343 96-97 22.624726769142885 24.82561596996178 26.345663849081426 26.203993411813908 98-99 22.422484267108185 24.94520515979362 26.252790067634635 26.37952050546356 100-101 22.23503592839257 24.885450240265335 26.372466598937393 26.5070472324047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 446.0 1 315.0 2 256.5 3 362.5 4 348.0 5 338.0 6 568.5 7 1162.5 8 1774.5 9 2045.0 10 1963.5 11 1860.0 12 1936.0 13 2147.5 14 2456.5 15 2761.5 16 3061.0 17 3341.0 18 3765.0 19 4305.0 20 4829.5 21 5559.0 22 6616.5 23 8004.0 24 10049.5 25 12747.0 26 16369.0 27 21107.5 28 26057.5 29 31598.0 30 37871.5 31 45970.5 32 56071.5 33 61774.5 34 65560.5 35 72540.0 36 81960.5 37 92091.0 38 101589.0 39 108946.5 40 115801.5 41 125367.0 42 134226.0 43 144372.0 44 154796.5 45 163608.5 46 172636.5 47 183307.5 48 194032.5 49 199550.5 50 202934.5 51 206611.0 52 209206.0 53 212720.0 54 213787.5 55 206128.0 56 192976.5 57 178075.5 58 165121.0 59 148994.5 60 126857.5 61 105106.0 62 85484.5 63 66308.5 64 49568.0 65 37120.5 66 26671.5 67 19833.5 68 14351.5 69 9803.0 70 7016.0 71 4938.5 72 3695.0 73 3088.5 74 2401.0 75 1677.0 76 1134.5 77 747.0 78 466.0 79 253.5 80 155.5 81 91.5 82 53.0 83 33.0 84 18.0 85 10.0 86 4.0 87 3.5 88 2.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023570863688914263 2 0.024872568140723462 3 4.0052444671052274E-5 4 0.0 5 0.0 6 4.0052444671052274E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.1040644620065514E-4 18-19 1.0013111167763069E-5 20-21 0.0 22-23 0.0 24-25 1.0013111167763069E-4 26-27 2.4031466802631365E-4 28-29 7.009177817434148E-5 30-31 3.404457797039443E-4 32-33 0.002182858234572349 34-35 0.00414542802345391 36-37 0.10099223923805832 38-39 0.09321205186070641 40-41 0.1025142321355583 42-43 0.10380592347619974 44-45 0.09707711277146296 46-47 0.09067873473526235 48-49 0.091750137630213 50-51 0.09094908873679196 52-53 0.0715236530713316 54-55 0.05977827367154552 56-57 0.03334366018865102 58-59 0.01457908986026303 60-61 0.010293478280460436 62-63 0.0018624386772039309 64-65 0.005256883363075611 66-67 0.15053711329615 68-69 0.4091657616483023 70-71 0.3904612699869209 72-73 0.1793548472369721 74-75 0.03040981861649644 76-77 0.0321020344038484 78-79 0.005477171808766399 80-81 0.001311717562976962 82-83 6.007866700657841E-5 84-85 3.804982243749966E-4 86-87 0.0010914291172861746 88-89 2.0026222335526138E-4 90-91 5.607342253947319E-4 92-93 0.002663487570624976 94-95 0.07948407644970325 96-97 0.017623075655263002 98-99 0.019355343887286013 100-101 0.010724042060674246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4993453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.977757095433 #Duplication Level Percentage of deduplicated Percentage of total 1 65.10327956840092 24.72476537564796 2 14.82961427327284 11.263909773786441 3 6.610841284951334 7.531947735490258 4 3.7569836625988637 5.707272517987594 5 2.307885132221307 4.382415047783103 6 1.5410945462338197 3.511638860277873 7 1.084850396396726 2.8840129387467344 8 0.7563791004576391 2.298046539939387 9 0.5966805070477986 2.0394528624216988 >10 3.102093497146373 20.766807065571363 >50 0.19919950179007542 5.215692373768159 >100 0.1044473770610778 6.979343045647629 >500 0.004856397931174231 1.2655854541016547 >1k 0.0016363937997019588 0.9944252489181052 >5k 1.5836069029351955E-4 0.4346851599119409 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9378 0.1878059130625641 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7259 0.14537034793358422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5046 0.1010523179050649 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.007866700657841E-5 2 0.0 0.0 0.0 0.0 6.007866700657841E-5 3 0.0 0.0 0.0 0.0 6.007866700657841E-5 4 0.0 0.0 0.0 0.0 6.007866700657841E-5 5 0.0 0.0 0.0 0.0 6.007866700657841E-5 6 0.0 0.0 0.0 0.0 1.0013111167763069E-4 7 0.0 0.0 0.0 0.0 1.0013111167763069E-4 8 0.0 0.0 0.0 0.0 1.0013111167763069E-4 9 0.0 0.0 0.0 2.0026222335526137E-5 1.0013111167763069E-4 10-11 0.0 2.0026222335526137E-5 0.0 3.0039333503289206E-5 1.2015733401315682E-4 12-13 0.0 2.0026222335526137E-5 0.0 4.0052444671052274E-5 4.8062933605262734E-4 14-15 0.0 2.0026222335526137E-5 0.0 5.006555583881534E-5 5.306948918914426E-4 16-17 0.0 2.0026222335526137E-5 0.0 1.0013111167763069E-4 5.80760447730258E-4 18-19 0.0 4.0052444671052274E-5 0.0 1.0013111167763069E-4 6.308260035690733E-4 20-21 0.0 4.0052444671052274E-5 0.0 2.1027533452302446E-4 7.309571152467041E-4 22-23 0.0 6.007866700657841E-5 0.0 3.9051133554275966E-4 7.609964487499932E-4 24-25 0.0 6.007866700657841E-5 0.0 9.011800050986762E-4 7.609964487499932E-4 26-27 0.0 6.007866700657841E-5 0.0 0.0017623075655263001 7.710095599177563E-4 28-29 0.0 6.007866700657841E-5 0.0 0.007409702264144671 7.910357822532824E-4 30-31 0.0 7.009177817434148E-5 0.0 0.028847773274325403 8.210751157565716E-4 32-33 0.0 8.010488934210455E-5 0.0 0.07889330289080522 8.61127560427624E-4 34-35 0.0 8.010488934210455E-5 0.0 0.15379137442567298 9.412324497697285E-4 36-37 0.0 8.010488934210455E-5 0.0 0.2767323533434679 9.412324497697285E-4 38-39 0.0 8.010488934210455E-5 0.0 0.4668012295299465 9.912980056085438E-4 40-41 0.0 8.010488934210455E-5 0.0 0.6809115856302241 0.001021337339111833 42-43 0.0 9.011800050986763E-5 0.0 0.8943710895045973 0.001031350450279596 44-45 0.0 1.2015733401315682E-4 0.0 1.1492247949465029 0.0010613897837828852 46-47 0.0 1.2015733401315682E-4 0.0 1.4438405648355959 0.0011114553396217007 48-49 0.0 1.4018355634868297E-4 0.0 1.759403763287649 0.0013017044518091989 50-51 0.0 1.602097786842091E-4 0.0 2.0980872354260667 0.0013818093411513035 52-53 0.0 1.8023600101973525E-4 0.0 2.4211001885869354 0.0014218617858223557 54-55 0.0 1.8023600101973525E-4 0.0 2.7671833498783305 0.0014218617858223557 56-57 0.0 1.8023600101973525E-4 0.0 3.149944537377242 0.001441888008157882 58-59 0.0 1.8023600101973525E-4 0.0 3.5473248671810866 0.0014819404528289342 60-61 0.0 1.8023600101973525E-4 0.0 3.9501523294601952 0.0015119797863322234 62-63 0.0 1.8023600101973525E-4 0.0 4.340843901003975 0.0015420191198355125 64-65 0.0 1.8023600101973525E-4 0.0 4.75241280933254 0.0015620453421710387 66-67 0.0 1.8023600101973525E-4 0.0 5.184258267775826 0.0015820715645065648 68-69 0.0 1.8023600101973525E-4 0.0 5.659450484464358 0.001602097786842091 70-71 0.0 1.8023600101973525E-4 0.0 6.19717458039557 0.0016321371203453802 72-73 0.0 2.0026222335526138E-4 0.0 6.751770768644462 0.001742281343190774 74-75 0.0 2.0026222335526138E-4 0.0 7.3074683991218095 0.0018524255660361678 76-77 0.0 2.0026222335526138E-4 0.0 7.900705183367101 0.0019225173442105092 78-79 0.0 2.0026222335526138E-4 0.0 8.550526058821422 0.0019625697888815616 80-81 0.0 2.0026222335526138E-4 0.0 9.222716224624524 0.002002622233552614 82-83 0.0 2.0026222335526138E-4 0.0 9.902115830468414 0.002052687789391429 84-85 0.0 2.0026222335526138E-4 0.0 10.573344737599413 0.002072714011726955 86-87 0.0 2.0026222335526138E-4 0.0 11.24990061987166 0.0021528189010690596 88-89 0.0 2.0026222335526138E-4 0.0 11.963444934797625 0.0022028844569078753 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6310 0.0 52.12733 1 GTATCAA 11700 0.0 42.311657 1 TATCAAC 14910 0.0 32.715626 2 TCAACGC 15080 0.0 32.24499 4 ATCAACG 15135 0.0 32.190502 3 CAACGCA 15285 0.0 31.78149 5 AACGCAG 15995 0.0 30.400406 6 ACGCAGA 19085 0.0 25.403788 7 GTACATG 18135 0.0 24.88998 1 CGCAGAG 19315 0.0 24.855675 8 TACATGG 17870 0.0 24.641275 2 ACATGGG 18405 0.0 22.759602 3 GCAGAGT 22220 0.0 21.072342 9 GGACCGA 1310 0.0 19.917332 6 TGGTATC 3510 0.0 19.331638 2 GAGTACT 10945 0.0 19.157827 12-13 AGAGTAC 19060 0.0 19.077831 10-11 GTGGTAT 3835 0.0 18.935972 1 CAGAGTA 20350 0.0 18.835913 10-11 CATGGGG 12140 0.0 18.483416 4 >>END_MODULE