FastQCFastQC Report
Fri 27 May 2016
SRR939863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947955
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33850.3570844607602681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.2578181453760991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16610.17521928783539303No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA12110.12774868005337806No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA10440.11013181005427473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.054.455071
GTATCAA29100.050.699831
TCAACGC37300.039.4268384
TATCAAC37850.039.104592
ATCAACG37850.038.979263
CGCACTA250.001560853338.26988690-91
CAACGCA38150.038.175345
AACGCAG40000.036.5302626
ACGCAGA44400.032.696447
CGCAGAG46050.031.5249068
GCAGAGT49100.028.7936539
GTATAGG2600.025.5442871
TACATGG30750.024.375362
AGAGTAC43950.023.90732410-11
TGGTATC6900.023.375932
ACATGGG30800.023.10363
CAGAGTA45700.023.09563810-11
GTACATG31250.022.9227121
GTGGTAT8400.022.025431
CATGGGG17100.021.9164434
Produced by FastQC (version 0.11.5)