FastQCFastQC Report
Fri 27 May 2016
SRR939863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947955
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.4384174354267871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.41120095363176523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.3086644408226129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.10359141520430823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.057.7183271
GTATCAA35700.037.3574451
TATCAAC48800.027.22682
TCAACGC49800.026.488064
ATCAACG50450.026.2415543
CAACGCA51700.025.514615
AACGCAG53550.024.7133056
TGGTATC9950.024.1498452
GTGGTAT11400.023.1495291
GACCGTG1754.3591044E-621.5295987
ACGCAGA62650.020.9733167
CGCAGAG62800.020.8482278
TAAGGTG5900.020.7607385
GCTACAC5050.020.5246473
GTAAGGT5750.020.4830024
GAGTACT45250.020.08788312-13
GGTAAGG5650.020.0127663
AGTACTT47600.019.68981212-13
AGAGTAC62550.019.46333310-11
GTACTTT46950.019.36103614-15
Produced by FastQC (version 0.11.5)