##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 947955 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.593722275846428 31.0 30.0 31.0 27.0 34.0 2 30.815329841606406 31.0 31.0 33.0 27.0 34.0 3 30.802283863685513 31.0 30.0 34.0 27.0 34.0 4 34.677226239642174 35.0 35.0 37.0 32.0 37.0 5 34.33608873838948 35.0 35.0 37.0 30.0 37.0 6 34.47214266499992 35.0 35.0 37.0 30.0 37.0 7 34.39198063199203 35.0 35.0 37.0 30.0 37.0 8 34.321218834227366 35.0 35.0 37.0 30.0 37.0 9 35.79383515040271 37.0 35.0 39.0 30.0 39.0 10-11 35.58336682648438 37.0 35.0 39.0 30.0 39.0 12-13 35.50201644592834 37.0 35.0 39.0 30.0 39.0 14-15 36.267867145592355 38.0 34.5 40.0 30.0 41.0 16-17 36.14346619829001 38.0 34.0 40.0 30.0 41.0 18-19 36.106845261642164 38.0 34.0 40.0 29.0 41.0 20-21 35.981294998180296 38.0 34.0 40.0 29.0 41.0 22-23 35.772342569003804 38.0 34.0 40.0 27.5 41.0 24-25 35.52752134858722 38.0 34.0 40.0 27.0 41.0 26-27 35.04200252121673 37.0 33.0 40.0 27.0 41.0 28-29 34.69425183684879 37.0 33.0 39.5 26.0 40.0 30-31 34.30070836695835 37.0 32.5 39.0 25.0 40.0 32-33 34.09902948979646 37.0 32.0 39.0 24.5 40.0 34-35 34.08578413532288 37.0 32.0 39.0 24.5 40.0 36-37 33.92549804579331 37.0 32.0 39.0 24.0 40.0 38-39 33.64967429888549 37.0 31.5 39.0 24.0 40.0 40-41 33.312007426512864 36.0 31.0 39.0 23.0 40.0 42-43 32.94609923466831 36.0 31.0 39.0 21.5 40.0 44-45 32.521185604801914 36.0 30.5 39.0 19.0 40.0 46-47 32.0157871417947 35.5 30.0 39.0 16.5 40.0 48-49 31.48618236097705 35.0 30.0 38.0 13.5 40.0 50-51 31.015511284818373 35.0 29.0 38.0 9.0 40.0 52-53 30.518163309439792 35.0 28.0 38.0 7.5 40.0 54-55 29.942212974244555 34.0 27.5 38.0 2.0 40.0 56-57 28.290024315500208 33.5 24.0 37.5 2.0 39.5 58-59 24.43990168309677 30.0 9.0 36.0 2.0 39.0 60-61 24.23508921837007 29.5 10.5 35.5 2.0 39.0 62-63 25.309443486241435 31.0 14.5 36.0 2.0 39.0 64-65 24.33073299892927 31.0 5.0 36.0 2.0 39.0 66-67 18.919772563043605 19.0 2.0 31.5 2.0 36.5 68-69 12.754143920333771 8.0 2.0 22.5 2.0 31.5 70-71 11.581693223834465 8.0 2.0 18.0 2.0 28.5 72-73 12.650614216919578 8.0 2.0 22.0 2.0 31.0 74-75 13.134676224082366 9.5 2.0 24.0 2.0 31.5 76-77 14.550758211096518 11.5 2.0 27.0 2.0 32.0 78-79 16.872450696499307 16.5 2.0 30.5 2.0 34.0 80-81 18.693403167871892 24.5 2.0 32.5 2.0 35.0 82-83 19.247596668618236 26.0 2.0 33.0 2.0 35.0 84-85 19.127958605630013 26.0 2.0 33.0 2.0 35.0 86-87 18.736438965984675 25.5 2.0 33.0 2.0 35.0 88-89 18.10027005501316 24.5 2.0 33.0 2.0 35.0 90-91 16.64032680876202 13.5 2.0 32.0 2.0 34.0 92-93 14.647730113771223 2.0 2.0 31.0 2.0 34.0 94-95 11.18038356251088 2.0 2.0 26.0 2.0 33.0 96-97 9.981205331476705 2.0 2.0 22.0 2.0 32.5 98-99 9.04786144911942 2.0 2.0 13.0 2.0 32.0 100-101 8.449637904752862 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32468388388915415 1101 2 0.1035119654245058 1101 3 0.052412948045077457 1101 4 0.10865232927056923 1101 5 0.6115440787700521 1101 6 0.41427736187362285 1101 7 0.22270406236339113 1101 8 0.24150867643594154 1101 9 0.44522365026907806 1101 10-11 0.4715378760268649 1101 12-13 0.10944754537673163 1101 14-15 0.0639987507006694 1101 16-17 0.04931169139426572 1101 18-19 0.20166097946403738 1101 20-21 -0.06554004317624162 1101 22-23 0.26181766158052255 1101 24-25 0.4182004040344225 1101 26-27 0.428494005453409 1101 28-29 0.6256875771225552 1101 30-31 0.6821240085754425 1101 32-33 0.7061518284547361 1101 34-35 0.5448647214911588 1101 36-37 0.410748970050264 1101 38-39 0.006169268131941408 1101 40-41 -0.08812524299374758 1101 42-43 0.5619476424433358 1101 44-45 0.28342339503761593 1101 46-47 -0.2860498069282933 1101 48-49 -7.327482933483509E-4 1101 50-51 -0.32887108173511237 1101 52-53 -0.13262113073427173 1101 54-55 -0.8091066067026205 1101 56-57 -0.7855754466103164 1101 58-59 -9.663035307114843 1101 60-61 -11.142580011869136 1101 62-63 -6.885786353442924 1101 64-65 -4.2489304234589085 1101 66-67 -4.319280447273117 1101 68-69 -5.258775742887358 1101 70-71 -4.901308483724916 1101 72-73 -6.598864615858453 1101 74-75 -7.633852915104311 1101 76-77 -8.449069092625223 1101 78-79 -10.443344801906864 1101 80-81 -9.64528532403541 1101 82-83 -8.592979568893506 1101 84-85 -7.741568134455495 1101 86-87 -7.439179389751638 1101 88-89 -7.641132487305281 1101 90-91 -9.845442815297755 1101 92-93 -10.885089222581332 1101 94-95 -8.644034046718426 1101 96-97 -7.649141017500252 1101 98-99 -6.803275644565319 1101 100-101 -6.199779349638901 1102 1 0.36413807241983775 1102 2 0.3690013182495768 1102 3 0.4042270597641924 1102 4 0.49383614749031324 1102 5 0.5692094563288776 1102 6 0.47730457221216227 1102 7 0.39653838258633556 1102 8 0.5017854334471323 1102 9 0.6150087811322464 1102 10-11 0.6167122800150793 1102 12-13 0.7136259129703788 1102 14-15 0.6898860152780273 1102 16-17 0.7447546149410726 1102 18-19 0.6051009182715319 1102 20-21 0.5585820529879939 1102 22-23 0.7856193529327982 1102 24-25 0.6240457859794191 1102 26-27 0.8344880894647204 1102 28-29 0.5972509814370497 1102 30-31 0.7784313297451604 1102 32-33 0.5728013783367985 1102 34-35 0.6882579100806865 1102 36-37 0.8637099063383786 1102 38-39 0.6662045180102467 1102 40-41 0.3540173505200599 1102 42-43 0.4699970778568954 1102 44-45 0.565696557524042 1102 46-47 0.4108667647937061 1102 48-49 0.2470982341565957 1102 50-51 0.07096229706467838 1102 52-53 0.1825630619151397 1102 54-55 0.009030231292364022 1102 56-57 -0.20917842279124343 1102 58-59 -8.935249684144118 1102 60-61 -9.771624520858122 1102 62-63 -4.997878559422034 1102 64-65 -2.5240305833938663 1102 66-67 -3.153192809335904 1102 68-69 -4.013184652173749 1102 70-71 -4.398722244549945 1102 72-73 -5.844719144585232 1102 74-75 -6.569418919030474 1102 76-77 -7.781256403738831 1102 78-79 -10.011406753969169 1102 80-81 -8.845843224233857 1102 82-83 -6.901517635017786 1102 84-85 -6.198050046828257 1102 86-87 -5.970002974541236 1102 88-89 -6.141966930555117 1102 90-91 -8.939109070091792 1102 92-93 -10.523371446265614 1102 94-95 -8.287035421410192 1102 96-97 -7.5255350993719725 1102 98-99 -6.759419232992233 1102 100-101 -6.2573599456239455 1103 1 0.07022705745337277 1103 2 0.24240752862099413 1103 3 0.23969493984258605 1103 4 0.29703627226737694 1103 5 0.1997181263003327 1103 6 0.2520033102006707 1103 7 0.039147715096866875 1103 8 -0.0167676951342699 1103 9 0.2131676413209007 1103 10-11 0.2693519252142096 1103 12-13 0.44018342231170493 1103 14-15 0.4317673685666463 1103 16-17 0.3562534315933874 1103 18-19 0.276960978083153 1103 20-21 0.2040554937833079 1103 22-23 0.2570701575414134 1103 24-25 0.4024444351320895 1103 26-27 0.20335349977103334 1103 28-29 0.3301395704390373 1103 30-31 0.34443534431152756 1103 32-33 0.28045482360781193 1103 34-35 0.4317379694854111 1103 36-37 -0.04665744004681471 1103 38-39 -0.1965207079786424 1103 40-41 -0.11586458596570282 1103 42-43 0.3518464231163918 1103 44-45 -0.21262173248769045 1103 46-47 -0.14426265750205758 1103 48-49 0.015338063808780333 1103 50-51 0.30010553977640697 1103 52-53 0.47799378012920357 1103 54-55 -0.05132321330051681 1103 56-57 0.31238359615588607 1103 58-59 -6.3708704839379635 1103 60-61 -8.42135157103342 1103 62-63 -3.7332199670971775 1103 64-65 -1.3139151646108473 1103 66-67 -2.721893093474975 1103 68-69 -4.09997048997713 1103 70-71 -4.343159154147607 1103 72-73 -6.464972021578104 1103 74-75 -6.502346500864561 1103 76-77 -7.822666452361197 1103 78-79 -10.011838387667114 1103 80-81 -8.623820750799844 1103 82-83 -6.857471331877337 1103 84-85 -6.09418975645033 1103 86-87 -5.8056831112534955 1103 88-89 -5.826392854346169 1103 90-91 -8.689454580491011 1103 92-93 -10.526782471404122 1103 94-95 -8.339873421450879 1103 96-97 -7.466809520752252 1103 98-99 -6.86348940652729 1103 100-101 -6.305811082519302 1104 1 0.02230994676353859 1104 2 -0.06453942416485603 1104 3 -0.04901247599866565 1104 4 0.20990079191844302 1104 5 -0.06197099391683736 1104 6 0.01842356737888906 1104 7 0.09025730562337486 1104 8 -0.011634600358227942 1104 9 0.1375674154766955 1104 10-11 0.2019991259052034 1104 12-13 0.13356738363401632 1104 14-15 -0.045659802285989315 1104 16-17 0.07328111246505387 1104 18-19 0.03570215838085744 1104 20-21 0.1355323428827333 1104 22-23 0.23663163091310935 1104 24-25 -0.046712575199158834 1104 26-27 -0.3355279731519616 1104 28-29 -0.18026982826946636 1104 30-31 0.059980829110045875 1104 32-33 -0.05481314203694865 1104 34-35 -0.013713924577196224 1104 36-37 -0.4768009109431972 1104 38-39 -0.1226984443765815 1104 40-41 -0.20150440932861358 1104 42-43 -0.25449030161916397 1104 44-45 -0.5792678338707944 1104 46-47 -0.6885617739426912 1104 48-49 -0.3473116421076661 1104 50-51 -0.6479944149799657 1104 52-53 -0.41713523898155813 1104 54-55 -0.8483876125511962 1104 56-57 -1.208659175894777 1104 58-59 -8.080885403116401 1104 60-61 -9.23521930260786 1104 62-63 -5.118322475687858 1104 64-65 -3.0366652291110547 1104 66-67 -4.1925053032707815 1104 68-69 -5.128364042199863 1104 70-71 -5.087586897089041 1104 72-73 -7.001973827583916 1104 74-75 -7.5388644618353915 1104 76-77 -8.751531510448775 1104 78-79 -10.829589592605773 1104 80-81 -9.796052009283022 1104 82-83 -7.749594828274674 1104 84-85 -7.1076304779552615 1104 86-87 -6.804100332248034 1104 88-89 -6.557544771965382 1104 90-91 -9.244893350163574 1104 92-93 -10.969517504551622 1104 94-95 -8.497611763739458 1104 96-97 -7.507080832929517 1104 98-99 -6.7882073817068616 1104 100-101 -6.291843515841044 1105 1 0.02625345945136104 1105 2 -0.0131412696664448 1105 3 -0.07439072069388786 1105 4 0.03531592586278265 1105 5 0.06835196886139272 1105 6 -0.04976439113888631 1105 7 -0.05522402114263514 1105 8 -0.14752732018698111 1105 9 -0.19575033084996107 1105 10-11 0.05178194694558158 1105 12-13 0.04667891386242218 1105 14-15 0.128609426884573 1105 16-17 0.1486429908032747 1105 18-19 0.1562910354928988 1105 20-21 0.2193436777539901 1105 22-23 0.04124720337190979 1105 24-25 0.08278037947381733 1105 26-27 -0.07377017445296019 1105 28-29 -0.201596750147381 1105 30-31 -0.3668005059690387 1105 32-33 0.0011572010850287029 1105 34-35 -0.07857145933622434 1105 36-37 -0.03131776569308187 1105 38-39 0.008898756197027069 1105 40-41 -0.2769779945479698 1105 42-43 -0.021515209655824208 1105 44-45 0.27776294527171075 1105 46-47 -0.09031816981109486 1105 48-49 0.624257657723998 1105 50-51 0.4450898899434499 1105 52-53 0.2671889084091781 1105 54-55 -0.11652179589595946 1105 56-57 -0.24299735159151226 1105 58-59 -7.33704616750321 1105 60-61 -8.994396152785551 1105 62-63 -4.896272576483835 1105 64-65 -2.5273286121149816 1105 66-67 -3.2130606683184766 1105 68-69 -4.514047636355775 1105 70-71 -4.390141087915568 1105 72-73 -6.156730722236533 1105 74-75 -6.647730611013436 1105 76-77 -7.7162711035239875 1105 78-79 -9.936389164482655 1105 80-81 -8.782709531572442 1105 82-83 -6.737426571733325 1105 84-85 -6.087949284198377 1105 86-87 -5.819705523760387 1105 88-89 -5.696393827392779 1105 90-91 -8.267520488181322 1105 92-93 -9.501665705811051 1105 94-95 -8.247035044492623 1105 96-97 -7.5522130107460885 1105 98-99 -6.779420206695951 1105 100-101 -6.226479403365204 1106 1 0.0657950736598174 1106 2 0.06297757490680311 1106 3 0.1465583176382168 1106 4 0.014780020691397056 1106 5 0.022962748726939708 1106 6 -0.07498392380550456 1106 7 -0.05383932231215738 1106 8 0.159361135839184 1106 9 -0.002262671396799476 1106 10-11 0.11603434814598756 1106 12-13 0.10689054810396925 1106 14-15 0.3352932958855277 1106 16-17 0.020309914812109753 1106 18-19 0.14302702618107332 1106 20-21 -0.052601670687309365 1106 22-23 0.21000180583764205 1106 24-25 0.08887196557259358 1106 26-27 0.2832980251812174 1106 28-29 0.06155195007669789 1106 30-31 0.07413006311348624 1106 32-33 0.14979506918330543 1106 34-35 0.2993592720698146 1106 36-37 0.042969495473585084 1106 38-39 -0.2744582328204075 1106 40-41 -0.12706677759664586 1106 42-43 -0.12756344349671167 1106 44-45 -0.0973568178151325 1106 46-47 -0.06998269459908713 1106 48-49 -0.22556141093489757 1106 50-51 -0.24276247087403036 1106 52-53 -0.3652977691846324 1106 54-55 -1.0600277662343025 1106 56-57 -0.7322661830996964 1106 58-59 -7.2476707369499636 1106 60-61 -9.232182792371905 1106 62-63 -4.857767259398692 1106 64-65 -2.881029588431584 1106 66-67 -3.408023591250437 1106 68-69 -4.392439109223991 1106 70-71 -4.401289942544955 1106 72-73 -5.875534417670096 1106 74-75 -6.497730314080163 1106 76-77 -7.790388617836172 1106 78-79 -9.940521485867395 1106 80-81 -8.832451793491344 1106 82-83 -6.714788378987999 1106 84-85 -5.828433562571341 1106 86-87 -5.474537419936679 1106 88-89 -5.178233388406628 1106 90-91 -7.877102722861546 1106 92-93 -9.04749141785704 1106 94-95 -8.099993687626338 1106 96-97 -7.352591699647119 1106 98-99 -6.641082458954623 1106 100-101 -6.309762258042101 1107 1 -0.00784992258883932 1107 2 -0.04537526843162354 1107 3 -0.005043461312670416 1107 4 -0.2181570109554869 1107 5 0.13297431913922253 1107 6 0.037221957218115165 1107 7 0.21920658872222276 1107 8 0.06060020948304867 1107 9 -0.21982935945667492 1107 10-11 -0.09542341080978645 1107 12-13 0.12461847618662603 1107 14-15 0.03044965464062699 1107 16-17 -0.039152851962725777 1107 18-19 -0.09737484225972537 1107 20-21 0.012077950924052061 1107 22-23 -0.0674943072269798 1107 24-25 -0.07370758101187391 1107 26-27 0.11217343534329416 1107 28-29 0.320677208829089 1107 30-31 -0.023965903513108344 1107 32-33 -0.05517297659213938 1107 34-35 -0.20874227637677478 1107 36-37 -0.189752285827538 1107 38-39 -0.1097256178168351 1107 40-41 -0.1837617977996686 1107 42-43 -0.3611298422962861 1107 44-45 -0.2719429269928142 1107 46-47 -0.049247476252752165 1107 48-49 -0.08096289520184641 1107 50-51 -0.27413817481610536 1107 52-53 -0.6653889539767732 1107 54-55 -0.47573493988992155 1107 56-57 -0.4070743221615878 1107 58-59 -6.970805685922635 1107 60-61 -8.931990479464568 1107 62-63 -4.8100065409484 1107 64-65 -2.6276578979762704 1107 66-67 -3.477278271391814 1107 68-69 -4.219319640197442 1107 70-71 -4.597303912913853 1107 72-73 -6.223994219526785 1107 74-75 -6.835359238303003 1107 76-77 -8.461383266520489 1107 78-79 -10.502152010061307 1107 80-81 -9.288716425995162 1107 82-83 -7.21709059725298 1107 84-85 -6.407849640924725 1107 86-87 -5.965165837953981 1107 88-89 -6.056048591747583 1107 90-91 -8.541042131866579 1107 92-93 -9.295527611552973 1107 94-95 -8.100348483471837 1107 96-97 -7.522941727217241 1107 98-99 -6.850469633875754 1107 100-101 -6.309002384774273 1108 1 -0.12179029232427752 1108 2 -0.11694316909418134 1108 3 -0.20344729766535607 1108 4 -0.03458545000474089 1108 5 -0.3269177255255542 1108 6 -0.05599029316413606 1108 7 -0.09678516404616033 1108 8 -0.15809390162291237 1108 9 0.015603620203243906 1108 10-11 -0.01924366286000634 1108 12-13 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-0.22052252433427455 2311 72-73 -1.1098693587168462 2311 74-75 -0.08947877570361662 2311 76-77 3.0094527529368147 2311 78-79 4.435466423586547 2311 80-81 3.492355923533058 2311 82-83 2.5154996565124783 2311 84-85 2.261863334519216 2311 86-87 2.127051239933845 2311 88-89 2.278900078865245 2311 90-91 3.4887811700146827 2311 92-93 4.722723379870963 2311 94-95 7.232340180108231 2311 96-97 7.582592420744234 2311 98-99 7.553569808191877 2311 100-101 6.669710082748047 2312 1 -0.032979185788601484 2312 2 -0.11475480507276714 2312 3 -0.1471086895119349 2312 4 -0.18961221628511282 2312 5 -0.2798973125600881 2312 6 -0.17253244270234092 2312 7 -0.27866056848171183 2312 8 -0.02664499286357369 2312 9 -0.01881016884316722 2312 10-11 -0.3797266324464488 2312 12-13 -0.34469270010848874 2312 14-15 -0.4781898501818844 2312 16-17 -0.16360224044836258 2312 18-19 -0.1146849247893087 2312 20-21 -0.2797722529140714 2312 22-23 -0.3266682656638906 2312 24-25 -0.14090331532628397 2312 26-27 -0.3200727613356378 2312 28-29 -0.44079738598049545 2312 30-31 -0.16524685210612944 2312 32-33 -0.11935712693517786 2312 34-35 -0.2597316370210976 2312 36-37 -0.23435430432212456 2312 38-39 -0.28273198154359136 2312 40-41 -0.10321590936376168 2312 42-43 0.10154789460339231 2312 44-45 0.21163194622981507 2312 46-47 0.07087532378702477 2312 48-49 0.08746208242567732 2312 50-51 0.22148676303812564 2312 52-53 0.2462956935324243 2312 54-55 0.37235221333668633 2312 56-57 1.5538931631720097 2312 58-59 4.854321091446359 2312 60-61 4.636203315901842 2312 62-63 3.085133659906724 2312 64-65 3.0424126996174436 2312 66-67 1.1956025578813296 2312 68-69 -0.7873829294487109 2312 70-71 0.39983365296066253 2312 72-73 5.353219412769128 2312 74-75 4.411411667533729 2312 76-77 4.839131300447791 2312 78-79 5.77607708410196 2312 80-81 4.133125326018362 2312 82-83 3.5350584034430526 2312 84-85 3.244600145293827 2312 86-87 3.0951255014116335 2312 88-89 3.065586836312473 2312 90-91 3.9093630585480597 2312 92-93 5.639025436075542 2312 94-95 7.9896385902081875 2312 96-97 8.254252018432556 2312 98-99 8.151869532873775 2312 100-101 7.44044203204983 2313 1 -0.21115845034426783 2313 2 -0.18790342611464794 2313 3 -0.18446824007373408 2313 4 -0.25535532149450546 2313 5 -0.5175888345212769 2313 6 -0.2675145673192958 2313 7 -0.4165436124040838 2313 8 -0.3752404984815527 2313 9 -0.5472831004059913 2313 10-11 -0.5462932820889534 2313 12-13 -0.5220931086886793 2313 14-15 -0.7652110452789245 2313 16-17 -0.4028897787527512 2313 18-19 -0.5707306346973695 2313 20-21 -0.539782467418668 2313 22-23 -0.3610783780234428 2313 24-25 -0.24142680868685318 2313 26-27 -0.5262014640935533 2313 28-29 -0.6267332899641573 2313 30-31 -0.5693198858139965 2313 32-33 -0.6139370554336452 2313 34-35 -0.686512791260121 2313 36-37 -1.011805785425281 2313 38-39 -1.228103534148289 2313 40-41 -1.4856724977192215 2313 42-43 -1.0229158439052952 2313 44-45 -1.0518001273146211 2313 46-47 -0.8095702839760008 2313 48-49 -1.0258040054190651 2313 50-51 -0.8654897436013016 2313 52-53 -1.057062324853682 2313 54-55 -1.0463403300749583 2313 56-57 0.008125798514196703 2313 58-59 3.4738946358794536 2313 60-61 3.410105256657719 2313 62-63 1.2751553657289918 2313 64-65 1.8414742420076387 2313 66-67 2.0024079715687684 2313 68-69 -0.010052745587627143 2313 70-71 1.4965969318166383 2313 72-73 6.5063859091940515 2313 74-75 8.554172137400942 2313 76-77 8.030742638547894 2313 78-79 6.503771159689297 2313 80-81 4.399557910288021 2313 82-83 3.381521631226505 2313 84-85 2.9744009624541974 2313 86-87 2.726215899777312 2313 88-89 2.714903741317581 2313 90-91 3.714368165800355 2313 92-93 5.054557867127638 2313 94-95 6.803638845570802 2313 96-97 6.67436820842234 2313 98-99 7.124283986397778 2313 100-101 5.8556999482908925 2314 1 -0.30959854557182 2314 2 -0.4780415614263518 2314 3 -0.33437688806398924 2314 4 -0.31920068745619545 2314 5 -0.3811919792450027 2314 6 -0.4628265482737888 2314 7 -0.46066524898918004 2314 8 -0.5571832512221135 2314 9 -0.479331046369893 2314 10-11 -0.5553572008133401 2314 12-13 -0.5436881701436675 2314 14-15 -0.7245268740907918 2314 16-17 -0.6604401607702499 2314 18-19 -0.7981922945357098 2314 20-21 -0.4829172254742531 2314 22-23 -0.5974821539065118 2314 24-25 -0.7639589203024002 2314 26-27 -0.7158260903807872 2314 28-29 -0.9079749852359953 2314 30-31 -1.0607175715630817 2314 32-33 -0.9817679988507635 2314 34-35 -1.4384346437681472 2314 36-37 -1.0756276213238607 2314 38-39 -0.9913142804665256 2314 40-41 -0.6209094592960085 2314 42-43 -0.4954604126130775 2314 44-45 -0.9818632432486254 2314 46-47 -0.8201304378902954 2314 48-49 -0.5315411884552717 2314 50-51 -0.25466591456796905 2314 52-53 -0.7809148882464427 2314 54-55 -0.3332520784970576 2314 56-57 1.1113847122557523 2314 58-59 3.982701800091615 2314 60-61 3.4578629800549017 2314 62-63 2.0025049117371836 2314 64-65 2.0152527549076495 2314 66-67 2.2783948500982767 2314 68-69 0.04544152414994329 2314 70-71 1.9388064047432252 2314 72-73 6.885347264525688 2314 74-75 8.70226620228855 2314 76-77 8.193046093314807 2314 78-79 6.766099528640684 2314 80-81 4.733464731238083 2314 82-83 3.6645647604480764 2314 84-85 3.094235421539569 2314 86-87 3.14199626739995 2314 88-89 3.4174775149860572 2314 90-91 3.969218127044954 2314 92-93 5.303170285464478 2314 94-95 7.484899918541679 2314 96-97 7.9164877134605405 2314 98-99 8.252783737255932 2314 100-101 7.531911054480682 2315 1 -0.6644639585090282 2315 2 -0.5471131507064086 2315 3 -0.5047765510656887 2315 4 -0.4996613171074742 2315 5 -0.6356975354717989 2315 6 -0.5602180769463985 2315 7 -0.6827543118922677 2315 8 -0.9178008201973711 2315 9 -1.2574281162480574 2315 10-11 -1.119046450502303 2315 12-13 -0.8356015171702751 2315 14-15 -0.9980726709186953 2315 16-17 -0.9142077168468319 2315 18-19 -0.738426527946217 2315 20-21 -0.6864068885739201 2315 22-23 -0.9365140348599041 2315 24-25 -1.0379994384699387 2315 26-27 -1.0296745325800245 2315 28-29 -0.9575263072685658 2315 30-31 -1.0664713603448277 2315 32-33 -1.3633475477563408 2315 34-35 -1.2596924768560243 2315 36-37 -1.5538786589324758 2315 38-39 -1.5775688643944505 2315 40-41 -1.1145964557986758 2315 42-43 -1.0527476742733164 2315 44-45 -1.378195447811816 2315 46-47 -1.4005814343537715 2315 48-49 -1.2382857261112399 2315 50-51 -1.489594057517948 2315 52-53 -1.0187885008224882 2315 54-55 -1.120505575017681 2315 56-57 0.21292259354222054 2315 58-59 3.6744765271785056 2315 60-61 3.5706341379640776 2315 62-63 1.4364138960726436 2315 64-65 1.7977851730337164 2315 66-67 2.1327851346201925 2315 68-69 4.680617191044108 2315 70-71 3.6986061324015758 2315 72-73 7.640117325231099 2315 74-75 9.094736968012688 2315 76-77 8.165135156217891 2315 78-79 6.495700845285203 2315 80-81 4.5853152829421795 2315 82-83 3.5578225086332793 2315 84-85 3.189286412125167 2315 86-87 3.0370524827653718 2315 88-89 2.7514364612785833 2315 90-91 3.6961254165924586 2315 92-93 5.077399385528807 2315 94-95 7.649841319370463 2315 96-97 7.895287352950735 2315 98-99 7.742400303726562 2315 100-101 6.433134142782727 2316 1 -0.7744160261018465 2316 2 -0.6110094867207074 2316 3 -0.6518924824979742 2316 4 -0.5511886548278397 2316 5 -1.1448615617940021 2316 6 -0.9307721430768723 2316 7 -0.7949527033131787 2316 8 -1.0275132257542765 2316 9 -0.8010709791938666 2316 10-11 -0.5559523729980356 2316 12-13 -0.8955779571735292 2316 14-15 -0.7597186210365052 2316 16-17 -1.1214503848133575 2316 18-19 -0.8917533619701032 2316 20-21 -1.040540043176243 2316 22-23 -0.9682753477204074 2316 24-25 -2.387260142020189 2316 26-27 -1.7201408580329414 2316 28-29 -1.3337408657217296 2316 30-31 -1.165153219147328 2316 32-33 -1.165262813009413 2316 34-35 -1.0158688518661805 2316 36-37 -1.0822603308798335 2316 38-39 -1.5142777765725342 2316 40-41 -1.3962785583252817 2316 42-43 -1.261552207541655 2316 44-45 -1.2305880061025007 2316 46-47 -1.3521839203396375 2316 48-49 -1.355728247843306 2316 50-51 -1.2813988345103944 2316 52-53 -0.9127441430354999 2316 54-55 -0.7592191179537444 2316 56-57 0.7859667076051018 2316 58-59 3.7666976661824876 2316 60-61 4.420711317263777 2316 62-63 2.553375062698688 2316 64-65 2.362875757159152 2316 66-67 6.652029183689979 2316 68-69 7.879530587745707 2316 70-71 4.878604507574213 2316 72-73 8.682143484951627 2316 74-75 9.83636910709791 2316 76-77 8.393242638547894 2316 78-79 6.96399843241657 2316 80-81 4.704482152712266 2316 82-83 3.7553852675901425 2316 84-85 3.3531888412420763 2316 86-87 3.2470492331106477 2316 88-89 3.6870628322266725 2316 90-91 4.4817924082245995 2316 92-93 5.719141200460971 2316 94-95 8.435646421328379 2316 96-97 8.865087905392034 2316 98-99 8.527882471246262 2316 100-101 7.388654493745436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 14.0 4 58.0 5 114.0 6 286.0 7 710.0 8 1550.0 9 2568.0 10 3997.0 11 5885.0 12 8134.0 13 11066.0 14 14709.0 15 20154.0 16 26078.0 17 31848.0 18 35697.0 19 38500.0 20 40705.0 21 40495.0 22 37045.0 23 33610.0 24 28313.0 25 25815.0 26 32485.0 27 46406.0 28 60621.0 29 69209.0 30 69765.0 31 64951.0 32 57298.0 33 48995.0 34 39934.0 35 28430.0 36 15863.0 37 5644.0 38 978.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.20367724795151 22.865366638038182 16.373101604390918 20.557854509619382 2 19.35362383970985 23.53894956489449 35.812838698175284 21.294587897220367 3 22.35770942583737 24.96231289632117 30.84350542282873 21.83647225501273 4 17.001123471050843 18.87505208580576 34.80186295762985 29.321961485513555 5 13.137187856836807 39.01222294688896 34.49948393878184 13.351105257492385 6 32.325374094761884 38.12659883644266 15.704331956685708 13.843695112109753 7 27.648675306317283 33.71098839079914 20.486731965124928 18.15360433775865 8 26.470827140164122 36.810580271384765 18.62687285129115 18.091719737159963 9 26.115902126155778 16.25478002647805 19.803788154501003 37.82552969286517 10-11 25.511179328132666 27.414961680670498 26.95486600102325 20.118992990173584 12-13 26.199397650732365 24.396252986692406 27.820782632086967 21.58356673048826 14-15 22.588778135624867 25.937467363888295 26.23505595741561 25.23869854307123 16-17 21.75055777964144 29.046315489659317 27.21938277660859 21.98374395409065 18-19 21.78681723838735 27.05164079044879 30.073712276852234 21.087829694311626 20-21 22.263662304645262 26.27967572300373 30.586789457305464 20.869872515045547 22-23 22.14081892072936 26.200821768965827 30.5400045360803 21.118354774224514 24-25 22.0849090937861 26.451255597575834 30.545911989493174 20.917923319144897 26-27 21.646303512617703 26.746392757454473 30.399629095926596 21.20767463400123 28-29 21.764237681229748 26.96164868105199 30.3916888684702 20.882424769248054 30-31 21.91568160874127 26.77773305769822 30.22039379810449 21.08619153545602 32-33 21.740522453164772 26.71150724035314 30.235634542291745 21.312335764190344 34-35 22.215347709679666 26.818510759051676 30.082567994379428 20.883573536889234 36-37 22.264233561239134 26.439815987169744 30.230068793787456 21.065881657803665 38-39 21.809134724953786 26.947945066725186 30.03266622211343 21.210253986207594 40-41 22.258219722268823 26.91841000431548 29.86976537086218 20.95360490255352 42-43 21.99088472217606 26.885427003654446 29.891495590343233 21.232192683826266 44-45 22.1452146259214 26.993897073254114 29.83290811212334 21.027980188701147 46-47 22.40261384667656 27.273398739292748 29.103840577722156 21.220146836308537 48-49 22.14883009311773 27.460814054709964 29.022712284681738 21.367643567490568 50-51 22.29329034075592 27.675768298003554 28.869635313984915 21.16130604725561 52-53 22.586475399458102 27.544356098013896 28.723844251432816 21.145324251095186 54-55 22.28177383142862 27.652496666047703 28.901568223636453 21.16416127888722 56-57 22.825268365744247 27.217351166993573 29.048459517781378 20.9089209494808 58-59 23.667498485869267 25.984291325665993 30.03546176600552 20.312748422459226 60-61 23.110702247012693 26.651508835484982 29.725254611359276 20.51253430614305 62-63 22.11132830425137 27.627424566407825 28.977175028719778 21.284072100621025 64-65 22.065551076050028 27.349582842378357 29.160136745272304 21.42472933629932 66-67 25.689481412302978 24.146816620595924 31.409197083888017 18.754504883213077 68-69 28.63817590793522 20.858263380769664 34.1582830388067 16.345277672488415 70-71 27.18929976736278 22.23731155575467 32.98104897256392 17.592339704318633 72-73 26.7020026663466 22.750384424316177 32.583412137851305 17.964200771485913 74-75 26.335350886979807 23.59835091272668 31.58873230975206 18.477565890541456 76-77 23.272734451195436 25.521050081844237 30.894279013088678 20.31193645387165 78-79 22.704000398445004 26.680449755396097 29.626399524833136 20.989150321325763 80-81 22.104758468783228 27.716645990514287 28.15928443634277 22.019311104359716 82-83 22.220311671734606 27.858463372373617 27.925660832161945 21.995564123729828 84-85 22.074993011271633 27.840878522714686 27.8741079481621 22.210020517851586 86-87 22.12461079237214 27.69317436765464 27.766862059489227 22.415352780483996 88-89 21.982384175611806 27.85476518124024 27.632971835673548 22.52987880747441 90-91 22.45036491140887 27.13481765002868 28.535411341462446 21.879406097100006 92-93 22.388219012900052 27.20424545026534 27.96526027055604 22.442275266278568 94-95 24.023463409832978 24.279793566498206 31.69223905889603 20.00450396477279 96-97 23.601774152827048 24.457558977957884 31.364709079160765 20.575957790054304 98-99 23.30009694686693 25.282335086340304 30.00281561857754 21.41475234821523 100-101 22.082837390660863 26.610972768046086 28.877874414867104 22.428315426425943 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 153.0 2 135.5 3 143.5 4 154.5 5 178.5 6 243.5 7 373.5 8 515.5 9 645.5 10 769.5 11 855.5 12 916.0 13 1000.5 14 1128.5 15 1294.0 16 1436.5 17 1570.0 18 1795.5 19 2005.5 20 2229.0 21 2614.0 22 3138.5 23 3699.5 24 4383.5 25 5323.0 26 6535.0 27 7908.0 28 9483.5 29 11200.0 30 12874.0 31 14799.0 32 17083.5 33 19285.0 34 21083.5 35 22780.0 36 24811.5 37 26626.0 38 28161.0 39 29402.0 40 30515.5 41 31658.5 42 32377.0 43 33135.0 44 34112.0 45 34820.5 46 34978.0 47 34941.0 48 34948.5 49 34553.0 50 33645.0 51 32902.0 52 31894.0 53 30371.0 54 28906.5 55 27076.5 56 24602.5 57 22022.0 58 19168.0 59 16194.0 60 13382.5 61 10685.5 62 8356.0 63 6418.0 64 4836.0 65 3510.0 66 2578.0 67 1969.0 68 1418.5 69 965.5 70 668.5 71 490.0 72 352.5 73 217.5 74 137.5 75 102.5 76 86.5 77 59.5 78 38.0 79 23.0 80 8.5 81 4.5 82 4.0 83 1.5 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019093733352321576 2 0.002531765748374132 3 0.0018988243112805989 4 0.0 5 0.041457664129626405 6 0.0 7 0.0 8 2.1098047903117762E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.1098047903117762E-4 16-17 0.0 18-19 0.0013186279939448602 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0549023951558881E-4 28-29 0.0022680401495851596 30-31 0.008808434999551666 32-33 4.2196095806235523E-4 34-35 9.494121556402994E-4 36-37 0.02057059670553982 38-39 0.01972667478941511 40-41 0.02152000886118012 42-43 0.021678244220453502 44-45 0.02246942101682042 46-47 0.024579225807132194 48-49 0.024262755088585428 50-51 0.020517851585782024 52-53 0.01803883095716569 54-55 0.013819221376542136 56-57 0.12722122885580012 58-59 0.80663111645595 60-61 0.2942122780089772 62-63 2.1098047903117762E-4 64-65 0.17616869999103332 66-67 1.5028139521390784 68-69 2.870600397698203 70-71 2.28069897832703 72-73 2.0009916082514465 74-75 1.6670622550648502 76-77 1.1402439989239996 78-79 0.4521839116835714 80-81 3.164707185467665E-4 82-83 2.6372559878897205E-4 84-85 0.0 86-87 0.003744903502803403 88-89 5.2745119757794404E-5 90-91 0.22970499654519466 92-93 0.1812849766075394 94-95 1.394897437114631 96-97 1.3923656713662569 98-99 1.0895031937170012 100-101 0.4267607639603146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 947955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.16041875755927 #Duplication Level Percentage of deduplicated Percentage of total 1 84.54269350389667 56.779226986136855 2 9.477544429988853 12.730317054228493 3 2.6397716252905776 5.31864503336514 4 1.1161195829399748 2.998362342950445 5 0.5682915868489119 1.9083350474585394 6 0.35030185657045193 1.411585162729321 7 0.22401287355026928 1.053135887630418 8 0.182781489847589 0.9820545119435716 9 0.12435517656480884 0.7516571159486505 >10 0.692188925862201 8.237842728565074 >50 0.05405317010910627 2.506536361251292 >100 0.025837670707597606 3.374356958257077 >500 0.0015754675561688502 0.7861613218275764 >1k 4.7264026685065505E-4 1.1617834877075086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.4384174354267871 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.41120095363176523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2926 0.3086644408226129 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.10359141520430823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0549023951558882E-4 0.0 12-13 0.0 0.0 0.0 1.0549023951558882E-4 0.0 14-15 0.0 0.0 0.0 1.0549023951558882E-4 0.0 16-17 0.0 0.0 0.0 1.0549023951558882E-4 0.0 18-19 0.0 0.0 0.0 1.5823535927338324E-4 0.0 20-21 0.0 0.0 0.0 2.6372559878897205E-4 1.0549023951558882E-4 22-23 0.0 0.0 0.0 4.747060778201497E-4 2.1098047903117764E-4 24-25 0.0 0.0 0.0 6.32941437093533E-4 2.1098047903117764E-4 26-27 0.0 0.0 0.0 8.96667035882505E-4 4.219609580623553E-4 28-29 0.0 0.0 0.0 0.0021625499100695706 4.219609580623553E-4 30-31 0.0 0.0 0.0 0.006540394849966507 4.219609580623553E-4 32-33 0.0 0.0 0.0 0.01782785047813451 4.747060778201497E-4 34-35 0.0 0.0 0.0 0.036183152153846966 5.274511975779441E-4 36-37 0.0 0.0 0.0 0.06867414592464832 5.274511975779441E-4 38-39 0.0 0.0 0.0 0.11872926457479523 5.274511975779441E-4 40-41 0.0 0.0 0.0 0.17833124990110288 5.274511975779441E-4 42-43 0.0 0.0 0.0 0.2424165704068231 5.274511975779441E-4 44-45 0.0 0.0 0.0 0.3187387586963516 5.274511975779441E-4 46-47 0.0 0.0 0.0 0.41025154147612497 5.274511975779441E-4 48-49 0.0 0.0 0.0 0.4998127548248599 5.274511975779441E-4 50-51 0.0 0.0 0.0 0.5968110300594438 5.274511975779441E-4 52-53 0.0 0.0 0.0 0.6868469494859988 5.274511975779441E-4 54-55 0.0 0.0 0.0 0.7842671856786452 5.274511975779441E-4 56-57 0.0 0.0 0.0 0.8562115290282766 5.274511975779441E-4 58-59 0.0 1.0549023951558882E-4 0.0 0.8957176237268647 5.274511975779441E-4 60-61 0.0 1.0549023951558882E-4 0.0 0.9217737128872151 5.801963173357385E-4 62-63 5.274511975779441E-5 1.0549023951558882E-4 0.0 0.9447178399818557 6.32941437093533E-4 64-65 1.0549023951558882E-4 1.0549023951558882E-4 0.0 0.9680839280345586 6.32941437093533E-4 66-67 1.0549023951558882E-4 1.0549023951558882E-4 0.0 0.9921884477638706 6.32941437093533E-4 68-69 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.0220421855467823 6.32941437093533E-4 70-71 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.07853220880738 6.32941437093533E-4 72-73 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.1623442041025154 6.32941437093533E-4 74-75 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.276431898138625 6.32941437093533E-4 76-77 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.458508051542531 6.32941437093533E-4 78-79 1.0549023951558882E-4 1.0549023951558882E-4 0.0 1.731727771887906 6.32941437093533E-4 80-81 1.0549023951558882E-4 1.0549023951558882E-4 0.0 2.0478820197161256 6.32941437093533E-4 82-83 1.0549023951558882E-4 1.0549023951558882E-4 5.274511975779441E-5 2.3406701794916422 7.384316766091217E-4 84-85 1.0549023951558882E-4 1.0549023951558882E-4 1.0549023951558882E-4 2.562727133671957 7.384316766091217E-4 86-87 1.0549023951558882E-4 1.0549023951558882E-4 1.0549023951558882E-4 2.754191918392751 7.911767963669162E-4 88-89 1.0549023951558882E-4 1.0549023951558882E-4 1.0549023951558882E-4 2.9407514069760694 8.439219161247106E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2180 0.0 57.718327 1 GTATCAA 3570 0.0 37.357445 1 TATCAAC 4880 0.0 27.2268 2 TCAACGC 4980 0.0 26.48806 4 ATCAACG 5045 0.0 26.241554 3 CAACGCA 5170 0.0 25.51461 5 AACGCAG 5355 0.0 24.713305 6 TGGTATC 995 0.0 24.149845 2 GTGGTAT 1140 0.0 23.149529 1 GACCGTG 175 4.3591044E-6 21.529598 7 ACGCAGA 6265 0.0 20.973316 7 CGCAGAG 6280 0.0 20.848227 8 TAAGGTG 590 0.0 20.760738 5 GCTACAC 505 0.0 20.524647 3 GTAAGGT 575 0.0 20.483002 4 GAGTACT 4525 0.0 20.087883 12-13 GGTAAGG 565 0.0 20.012766 3 AGTACTT 4760 0.0 19.689812 12-13 AGAGTAC 6255 0.0 19.463333 10-11 GTACTTT 4695 0.0 19.361036 14-15 >>END_MODULE