FastQCFastQC Report
Fri 27 May 2016
SRR939862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023368
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.3384901618967957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26470.2586557328351092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.18761579412293525No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA13740.13426255266922554No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA11730.11462152422198074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.059.1676831
GTATCAA29200.042.5520971
TATCAAC38500.032.5177762
TCAACGC38600.032.4335334
ATCAACG38750.032.1865233
CAACGCA39700.031.4163175
AACGCAG41100.030.5752036
TGGTATC6650.027.6021462
GTGGTAT7600.027.2482721
ACGCAGA46900.026.5933427
CGCAGAG47350.026.141818
GTAAGGT3700.025.4406494
TACATGG32650.025.226372
GTACATG34900.024.544031
TAAGGTG4450.023.2681885
ACATGGG34500.023.0551263
GCAGAGT50700.022.9291979
ACCGTGC1954.611138E-721.72248
CAGAGTA49150.020.73166810-11
GAGTACT33200.020.62545812-13
Produced by FastQC (version 0.11.5)