FastQCFastQC Report
Fri 27 May 2016
SRR939862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023368
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47220.46141759367109386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.4164679763291407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.30585283104416006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.10719506570461457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.061.3916171
GTATCAA40350.039.3933681
TGGTATC8350.038.0612342
GTGTACG550.002593269334.5079541
GTGGTAT11100.031.632291
TAAGGTG5300.029.5318165
TATCAAC56750.028.2517532
TAGCGCG856.462271E-427.8999334
ATCAACG57700.027.7016853
TCAACGC57900.027.6059994
TAACCCG700.00845182327.1027935
CAACGCA59800.026.8081975
AACGCAG62450.025.5946716
GTAAGGT5200.025.5391714
CGTATTG1000.001667799223.7149435
CGTACAC1000.001667799223.7149433
GTGTAGC6600.023.00531
ACGCAGA71600.022.3900877
GGTAAGG5950.022.3199483
CGCAGAG72150.022.153678
Produced by FastQC (version 0.11.5)