##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1023368 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.568529600300185 31.0 30.0 31.0 27.0 34.0 2 30.817379476395587 31.0 31.0 33.0 27.0 34.0 3 30.787565176945147 31.0 30.0 34.0 27.0 34.0 4 34.68090364365507 35.0 35.0 37.0 32.0 37.0 5 34.32653063218705 35.0 35.0 37.0 30.0 37.0 6 34.46842093948609 35.0 35.0 37.0 30.0 37.0 7 34.3771605131292 35.0 35.0 37.0 30.0 37.0 8 34.344589629536976 35.0 35.0 37.0 30.0 37.0 9 35.78983415545532 37.0 35.0 39.0 30.0 39.0 10-11 35.588396353999734 37.0 35.0 39.0 30.0 39.0 12-13 35.483591435339 37.0 34.5 39.0 30.0 39.0 14-15 36.239564360034706 38.0 34.5 40.0 30.0 41.0 16-17 36.099504283893964 38.0 34.0 40.0 29.0 41.0 18-19 36.06142365209777 38.0 34.0 40.0 29.0 41.0 20-21 35.93104972991143 38.0 34.0 40.0 29.0 41.0 22-23 35.749975082277345 38.0 34.0 40.0 27.5 41.0 24-25 35.50648837954675 38.0 34.0 40.0 27.0 41.0 26-27 35.030551570891404 37.0 33.0 40.0 27.0 41.0 28-29 34.68955302491381 37.0 33.0 39.5 26.0 40.0 30-31 34.28038252124358 37.0 32.5 39.0 25.0 40.0 32-33 34.101561706052955 37.0 32.0 39.0 25.0 40.0 34-35 33.8373737502052 36.0 32.0 39.0 24.0 40.0 36-37 33.78247121270159 37.0 32.0 39.0 24.0 40.0 38-39 33.538383553130444 36.0 31.0 39.0 24.0 40.0 40-41 33.31653178524246 36.0 31.0 39.0 23.0 40.0 42-43 32.93602643428366 36.0 31.0 39.0 21.5 40.0 44-45 32.49884108160505 36.0 30.0 39.0 19.0 40.0 46-47 32.041775783491374 35.5 30.0 39.0 16.5 40.0 48-49 31.52884934842598 35.0 30.0 38.0 14.0 40.0 50-51 31.151106933185325 35.0 29.0 38.0 10.5 40.0 52-53 30.720555557727035 35.0 28.0 38.0 8.5 40.0 54-55 30.268855387309355 34.0 28.0 38.0 8.0 40.0 56-57 29.679037257369785 34.0 26.5 38.0 7.0 40.0 58-59 29.085295318985935 33.5 26.0 38.0 2.0 40.0 60-61 28.575354124811405 33.0 26.0 37.0 2.0 39.0 62-63 28.13684324700401 33.0 25.0 37.0 2.0 39.0 64-65 27.488627746812487 32.0 23.5 36.0 2.0 39.0 66-67 26.258919078962798 31.5 20.0 35.5 2.0 38.5 68-69 23.989922002642256 29.0 10.5 34.0 2.0 38.0 70-71 23.200812904057972 28.0 10.0 34.0 2.0 37.0 72-73 23.578529912993176 28.5 10.5 34.0 2.0 36.5 74-75 23.65589797609462 29.0 13.0 34.0 2.0 36.0 76-77 23.862469317000336 29.5 16.0 33.5 2.0 36.0 78-79 24.399589395017237 30.0 18.0 34.0 2.0 36.0 80-81 24.132658535346035 30.0 14.5 34.0 2.0 36.0 82-83 23.502449754145136 29.5 8.5 34.0 2.0 35.0 84-85 22.9356536456094 29.0 7.0 34.0 2.0 35.0 86-87 22.320517155119177 29.0 2.0 34.0 2.0 35.0 88-89 21.575358033473783 29.0 2.0 33.0 2.0 35.0 90-91 20.75633154446885 27.0 2.0 33.0 2.0 34.5 92-93 19.903091067924734 26.0 2.0 32.5 2.0 34.0 94-95 18.718680865534196 25.0 2.0 32.0 2.0 34.0 96-97 17.771140977634634 23.5 2.0 32.0 2.0 34.0 98-99 16.76111477005339 19.0 2.0 32.0 2.0 34.0 100-101 15.29094372698775 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.22333421310259993 1101 2 0.21732713280165683 1101 3 0.3057681850679792 1101 4 0.19626983266770992 1101 5 0.25452500561489444 1101 6 0.14811392885548003 1101 7 -0.0010555667628366905 1101 8 -0.02283598102876283 1101 9 0.4092990502919065 1101 10-11 0.3376821504499574 1101 12-13 0.3012421189778962 1101 14-15 0.2741712490830679 1101 16-17 0.26310473876846885 1101 18-19 0.17925015582205361 1101 20-21 0.4442135112109682 1101 22-23 0.2244043120910746 1101 24-25 0.10246172171282097 1101 26-27 0.1148943446893469 1101 28-29 0.02544794482810886 1101 30-31 -0.0026301050582091534 1101 32-33 0.09244380910983807 1101 34-35 0.054085640322227846 1101 36-37 0.4480663163880152 1101 38-39 0.41328646884311127 1101 40-41 0.5206593643218653 1101 42-43 0.24665498058578095 1101 44-45 0.31913312844169894 1101 46-47 0.08377435572117164 1101 48-49 0.19725283475836974 1101 50-51 0.14584726283635163 1101 52-53 -0.32897385120018185 1101 54-55 -0.7087609803358106 1101 56-57 -0.48678713590172507 1101 58-59 -0.45385438661804045 1101 60-61 -0.7623248533521298 1101 62-63 -0.4562861442141788 1101 64-65 -0.7430927823337186 1101 66-67 -1.7361607821552845 1101 68-69 -8.436799726108202 1101 70-71 -7.6768060750791705 1101 72-73 -3.434164801281149 1101 74-75 -1.900013530353089 1101 76-77 -1.445582530219216 1101 78-79 -1.7804012944039123 1101 80-81 -1.6831912843822785 1101 82-83 -1.5808113435289677 1101 84-85 -1.51561046533768 1101 86-87 -1.6456509865237585 1101 88-89 -1.3120810890304782 1101 90-91 -1.720912250049551 1101 92-93 -1.7104692312298404 1101 94-95 -1.3063895945050987 1101 96-97 -2.1130823505606724 1101 98-99 -2.0634385165035187 1101 100-101 -1.8284405207711796 1102 1 0.2095229908795524 1102 2 0.10771661199857263 1102 3 0.27686005222012966 1102 4 0.2965038875254038 1102 5 0.19243593570372042 1102 6 0.2369039918244269 1102 7 0.2295368675317846 1102 8 0.3004426733171428 1102 9 0.42142435775262754 1102 10-11 0.3148524942350903 1102 12-13 0.23097757245608364 1102 14-15 0.25378483518571215 1102 16-17 0.29571263855426366 1102 18-19 0.26428584732633453 1102 20-21 0.08039285729693546 1102 22-23 0.16896241254850963 1102 24-25 0.2754342804561247 1102 26-27 0.19747593502136596 1102 28-29 0.03478190611349419 1102 30-31 -0.07888067282119238 1102 32-33 -0.04475471805715614 1102 34-35 -0.07872497876900297 1102 36-37 0.02935415174047762 1102 38-39 0.35913832428011006 1102 40-41 0.10922108108818662 1102 42-43 0.17630077094906937 1102 44-45 -0.017317316709302588 1102 46-47 9.467347659430914E-4 1102 48-49 0.25889234808311556 1102 50-51 0.04263695101968423 1102 52-53 0.0069811199214697695 1102 54-55 -0.4836563009760724 1102 56-57 -0.4142081020543671 1102 58-59 -0.3177876623684206 1102 60-61 -0.057118870185213666 1102 62-63 -0.43744634148544037 1102 64-65 -0.3205707109358187 1102 66-67 0.07657857802858459 1102 68-69 -5.069555120166733 1102 70-71 -0.8058734918134718 1102 72-73 0.20999611329503054 1102 74-75 0.17456477377922752 1102 76-77 -0.16462083281268391 1102 78-79 -0.19301264420299447 1102 80-81 -0.23220163938616167 1102 82-83 -0.36806993567535073 1102 84-85 -0.6517870191467203 1102 86-87 -0.9641200989768173 1102 88-89 -0.5557174898971979 1102 90-91 -0.9756094803590329 1102 92-93 -1.4548800235663428 1102 94-95 -0.8828213500158135 1102 96-97 -1.3063879596865782 1102 98-99 -1.350589140608438 1102 100-101 -1.106926399295963 1103 1 0.16229269507566002 1103 2 0.09955910275136759 1103 3 0.21581121704489092 1103 4 0.056084031977114535 1103 5 0.23199847420104902 1103 6 0.1229280411562641 1103 7 0.18601047775077717 1103 8 0.15702823580666347 1103 9 0.2534833942096313 1103 10-11 0.24797875040172812 1103 12-13 0.2109803100438512 1103 14-15 0.02124496278493382 1103 16-17 -0.010746498866986087 1103 18-19 0.13221795766116884 1103 20-21 0.08420727145001194 1103 22-23 0.1349852437793615 1103 24-25 0.1511999102928172 1103 26-27 0.20200519864165045 1103 28-29 -0.20414200841097596 1103 30-31 0.22380392127805493 1103 32-33 0.08715337434019688 1103 34-35 -0.30216990647722497 1103 36-37 0.2251051769231438 1103 38-39 0.19222646769956953 1103 40-41 0.15207531772502847 1103 42-43 -0.15487985145029626 1103 44-45 -0.22537084785218298 1103 46-47 -0.2649213850826406 1103 48-49 -0.3495500335429007 1103 50-51 -0.5211642272732533 1103 52-53 -0.6645642295385166 1103 54-55 -0.8894107607011996 1103 56-57 -0.53058572089299 1103 58-59 -0.7478039937119796 1103 60-61 -0.8321029689443229 1103 62-63 -1.0174146708599459 1103 64-65 -1.211055898137218 1103 66-67 -0.9895404455065098 1103 68-69 -5.457817900965697 1103 70-71 -1.2206773744161659 1103 72-73 -0.33130818402709394 1103 74-75 -0.4163994284933459 1103 76-77 -0.8031547038685325 1103 78-79 -0.7911633283173849 1103 80-81 -1.0346523455393921 1103 82-83 -1.0044137365145822 1103 84-85 -1.0730047685105113 1103 86-87 -0.9898465172639028 1103 88-89 -1.0534457943571454 1103 90-91 -1.221140336928901 1103 92-93 -1.1049889933731372 1103 94-95 -1.4132644562235406 1103 96-97 -1.6669574658470943 1103 98-99 -1.3639063121277317 1103 100-101 -1.1237748078847059 1104 1 0.21698266516890286 1104 2 0.17014383942160904 1104 3 0.04592840798682474 1104 4 0.3493600656261009 1104 5 0.36100051117761467 1104 6 0.14939448287437074 1104 7 0.05201059177999667 1104 8 0.17585990275840402 1104 9 0.34905154481113954 1104 10-11 0.28430353672651876 1104 12-13 0.32699662140582575 1104 14-15 0.4404358217878013 1104 16-17 0.15218571076018605 1104 18-19 0.04657067337076626 1104 20-21 0.1815321847618847 1104 22-23 0.050309151560824716 1104 24-25 0.15667525665657678 1104 26-27 0.4247000590336043 1104 28-29 0.12606491575441225 1104 30-31 -0.058363657622393816 1104 32-33 0.07804269861339463 1104 34-35 0.10640651642606969 1104 36-37 0.18962362374146835 1104 38-39 0.10237435582654086 1104 40-41 0.18521311685736208 1104 42-43 0.11640214800029014 1104 44-45 0.2621497551550789 1104 46-47 0.24885185485039685 1104 48-49 0.44799043387324744 1104 50-51 0.3170739823642883 1104 52-53 0.2108500982332977 1104 54-55 0.04265147074702824 1104 56-57 0.001913255435582073 1104 58-59 -0.12263780907793276 1104 60-61 -0.19966094243770982 1104 62-63 0.09001996966292936 1104 64-65 0.29128753196545887 1104 66-67 0.11919808030296863 1104 68-69 -4.297241107467432 1104 70-71 0.21367710356323855 1104 72-73 0.7241693003845953 1104 74-75 0.6929354831080907 1104 76-77 0.6115404234554624 1104 78-79 0.21672898403975083 1104 80-81 0.3404195706180282 1104 82-83 0.2968598921544334 1104 84-85 0.33368369328710656 1104 86-87 0.25352715390691927 1104 88-89 0.05323664203269374 1104 90-91 0.33854003138548805 1104 92-93 0.18555746576034338 1104 94-95 0.5975048263771541 1104 96-97 0.042081099354302154 1104 98-99 0.30283194674970204 1104 100-101 0.4081520786510353 1105 1 0.020358171326808616 1105 2 0.08735866879809251 1105 3 -0.04290335436678916 1105 4 -0.08492971712504982 1105 5 -0.026380405199212476 1105 6 -0.1947466868754475 1105 7 -0.256808737151232 1105 8 -0.037679926198030955 1105 9 0.08883597615008654 1105 10-11 0.18568885520695488 1105 12-13 0.00956000665710377 1105 14-15 0.11010583203697166 1105 16-17 -0.011792648364099989 1105 18-19 -0.22055153540992478 1105 20-21 -0.09280434933761228 1105 22-23 -0.22955521492270492 1105 24-25 -0.21398984743132843 1105 26-27 -0.2736917869935809 1105 28-29 -0.23907130582934144 1105 30-31 -0.25163721811771467 1105 32-33 0.050903393876929215 1105 34-35 -0.009465839371436857 1105 36-37 -0.24620878548614655 1105 38-39 0.14119414681062636 1105 40-41 0.05427783786144147 1105 42-43 0.08358379358054435 1105 44-45 0.10859173811792999 1105 46-47 -0.04958073583798139 1105 48-49 -0.0035759253182412465 1105 50-51 0.17137926488861055 1105 52-53 -0.5009853857460662 1105 54-55 -0.2681515351168464 1105 56-57 -0.006842646239569206 1105 58-59 0.0917851709109776 1105 60-61 -0.16829070368239485 1105 62-63 -0.1438071301572883 1105 64-65 -0.251836701446841 1105 66-67 0.458710320503247 1105 68-69 1.6003081221644386 1105 70-71 1.5456694334857808 1105 72-73 0.8294763975165047 1105 74-75 0.4000307248037416 1105 76-77 0.07387261425808944 1105 78-79 -0.08749816954661682 1105 80-81 -0.03146157258475313 1105 82-83 0.035856733393071494 1105 84-85 -0.027509327194433553 1105 86-87 -0.0752329062439614 1105 88-89 0.046682842277999015 1105 90-91 -0.4284729649012853 1105 92-93 -0.12994062722149025 1105 94-95 0.07376791087402168 1105 96-97 -0.46710010097502064 1105 98-99 -0.26795140086526104 1105 100-101 -0.07890624082071263 1106 1 0.4689008237366785 1106 2 0.4740535675640736 1106 3 0.38006689429922247 1106 4 0.3997413292426586 1106 5 0.6041330298855812 1106 6 0.4723318703897661 1106 7 0.27195944088485646 1106 8 0.40923586183768634 1106 9 0.4273645125102945 1106 10-11 0.4207769666782113 1106 12-13 0.4491549050091237 1106 14-15 0.6894175400789777 1106 16-17 0.6844628728327464 1106 18-19 0.6377601684924841 1106 20-21 0.3851470848281977 1106 22-23 0.4877025871959404 1106 24-25 0.49208067486251394 1106 26-27 0.6473307779878539 1106 28-29 0.8727673406857406 1106 30-31 0.928724293500089 1106 32-33 0.8471769223512027 1106 34-35 1.0416272230662074 1106 36-37 1.2262876855014397 1106 38-39 1.2427965875211413 1106 40-41 0.7764203727065606 1106 42-43 0.8041211969725381 1106 44-45 1.0340250511671982 1106 46-47 0.9472214200677875 1106 48-49 0.8153888425120677 1106 50-51 1.1678305893549101 1106 52-53 0.783071136407024 1106 54-55 0.6774613125581475 1106 56-57 0.7026213164377602 1106 58-59 0.7744979776038896 1106 60-61 0.7253473459017776 1106 62-63 0.8795257478196028 1106 64-65 0.9375056810608484 1106 66-67 1.5258950586112014 1106 68-69 3.08318775489877 1106 70-71 3.2146894650295614 1106 72-73 2.3119368953452835 1106 74-75 2.060103002289676 1106 76-77 1.4160895377871832 1106 78-79 1.2835862825146975 1106 80-81 1.3828558688088037 1106 82-83 1.5604646094036525 1106 84-85 1.4900136165607627 1106 86-87 1.4560645698394765 1106 88-89 1.5989349938371795 1106 90-91 1.4684715933822723 1106 92-93 1.7029156167502109 1106 94-95 1.4609129915756007 1106 96-97 1.4670657067238189 1106 98-99 1.5289792897448713 1106 100-101 1.4777504494002986 1107 1 0.10186085887062646 1107 2 0.07829891963551461 1107 3 0.10269608475450198 1107 4 0.03395008345141548 1107 5 0.023709958592945668 1107 6 0.18510452446676595 1107 7 0.13565289718700768 1107 8 0.11051825094616419 1107 9 0.12183823524488702 1107 10-11 0.18420252662550496 1107 12-13 -0.1302939311788407 1107 14-15 0.12471983842475254 1107 16-17 0.04774423720106569 1107 18-19 -0.18963699151336755 1107 20-21 -0.03686799349123504 1107 22-23 -0.06752358759544563 1107 24-25 0.1388410320576483 1107 26-27 -0.08549750484042562 1107 28-29 -0.13778089529728277 1107 30-31 -0.08333543421181844 1107 32-33 0.23855666177441748 1107 34-35 0.1028473958081193 1107 36-37 0.001514592250082103 1107 38-39 0.3870638983102026 1107 40-41 0.08553397247234074 1107 42-43 0.03704683011555687 1107 44-45 0.027252250698751368 1107 46-47 0.02988720838574821 1107 48-49 0.029541236012288863 1107 50-51 -0.3175383171009507 1107 52-53 -0.0314033182848199 1107 54-55 -0.19774217155838159 1107 56-57 -0.03221033339388768 1107 58-59 0.07660015883650217 1107 60-61 0.2249008519770257 1107 62-63 -0.105596489041762 1107 64-65 0.0057943650017051596 1107 66-67 0.5405320078133684 1107 68-69 1.868764537214684 1107 70-71 2.1434290346386966 1107 72-73 1.5091737567125811 1107 74-75 1.0261306702738686 1107 76-77 0.8164394133544484 1107 78-79 0.7082664171948316 1107 80-81 0.659332226589509 1107 82-83 0.6303328570979865 1107 84-85 0.6832766819977429 1107 86-87 0.506386631031102 1107 88-89 0.5969784720980513 1107 90-91 1.0361410414864984 1107 92-93 0.8670072577983738 1107 94-95 1.1851934775952166 1107 96-97 0.7076856745176947 1107 98-99 0.8763420477598025 1107 100-101 0.6287224886332101 1108 1 -0.2105459965690173 1108 2 -0.24385085681754148 1108 3 -0.0720133042171831 1108 4 -0.19562145157094335 1108 5 -0.04580196391077607 1108 6 -0.04367714468375539 1108 7 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-0.190052457751694 2316 92-93 -0.2951131078694793 2316 94-95 0.20680851060689776 2316 96-97 0.34413818848417677 2316 98-99 0.15482604979129277 2316 100-101 -0.032189141635962315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 58.0 5 133.0 6 298.0 7 777.0 8 1654.0 9 2702.0 10 4203.0 11 6281.0 12 8288.0 13 10590.0 14 13157.0 15 16145.0 16 18858.0 17 20950.0 18 19993.0 19 18907.0 20 18918.0 21 19402.0 22 20177.0 23 21876.0 24 24743.0 25 29107.0 26 34783.0 27 40846.0 28 47825.0 29 54422.0 30 61327.0 31 67885.0 32 74082.0 33 80540.0 34 83798.0 35 81118.0 36 67602.0 37 40726.0 38 10817.0 39 355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.55510815216754 22.80910253052225 16.316551796932725 20.319237520377488 2 19.402092383470073 23.38885984158087 35.923321622110265 21.28572615283879 3 22.27918011897968 25.148236020669007 30.870224591740214 21.702359268611097 4 17.015091345439764 18.919782522025315 34.79735539903534 29.26777073349958 5 13.208054189695204 38.85542639597877 34.4890596540052 13.447459760320823 6 32.196140586768394 38.23355821170879 15.735688432704558 13.834612768818255 7 27.665414591818386 33.742700573009905 20.540704809999923 18.05118002517179 8 26.533465967276676 36.800251717857115 18.688291992714255 17.977990322151953 9 26.227906285910834 16.193295080557533 19.848187553255524 37.730611080276105 10-11 25.461466451950816 27.416872522885217 26.958581859116173 20.163079166047794 12-13 26.178901431352163 24.46089774157488 27.80773876064133 21.552462066431627 14-15 22.587915588527295 25.902607859538307 26.26171621547674 25.24776033645766 16-17 21.74071727061348 29.034250226336532 27.225903159721526 21.99912934332846 18-19 21.81067807474926 27.032748727730393 30.11893082449324 21.0376423730271 20-21 22.271558227343437 26.212955652316666 30.716565302022342 20.798920818317555 22-23 22.016175999249537 26.173282729184415 30.653440404624728 21.157100866941313 24-25 22.100939448838442 26.468291891659483 30.555881278056923 20.874887381445152 26-27 21.555798761927562 26.777396139207422 30.519902478587795 21.14690262027722 28-29 21.699009250835722 26.915417440664 30.57695821733552 20.808615091164754 30-31 21.834714644405434 26.78461758700599 30.35324202983593 21.027425738752655 32-33 21.64417749838886 26.797769470593725 30.251249701346904 21.306803329670508 34-35 22.235979019526887 26.91485905118815 30.094470760969678 20.754691168315283 36-37 22.342341901823392 26.396882288799027 30.254560923969116 21.00621488540847 38-39 21.812806908687836 26.993100406660886 30.066404490920906 21.12768819373037 40-41 22.16187115263969 26.966887702187357 29.95690614164496 20.914335003527995 42-43 21.97040191876542 26.91244532569869 29.870297521105687 21.2468552344302 44-45 22.103348458770146 27.021006102331402 29.895507354091695 20.98013808480676 46-47 22.36759755995001 27.30113964181568 29.138853630249113 21.1924091679852 48-49 22.14579364675786 27.53731678981729 29.05394701328775 21.2629425501371 50-51 22.260746009805395 27.64315096657981 28.985268440608035 21.110834583006756 52-53 22.554105379665305 27.513579815976446 28.807982795396136 21.124332008962117 54-55 22.27882901181068 27.65471422614657 28.94963120041101 21.116825561631742 56-57 22.329876451499395 27.848848574891505 28.616628220667007 21.204646752942097 58-59 22.344574365209013 27.473477383639366 28.693925757686074 21.488022493465547 60-61 22.13578390126584 27.714891333984188 28.83907813457406 21.310246630175918 62-63 22.042339334206932 27.711899436263803 29.00857086180597 21.237190367723294 64-65 21.86958410309205 27.677160141246347 28.938351060788992 21.514904694872612 66-67 22.162518050762415 27.68617372788017 28.649533739628456 21.501774481728958 68-69 23.168733232530034 26.632942249923758 29.41852145229432 20.779803065251883 70-71 23.049262060731735 26.603957538055518 29.282669227264872 21.06411117394788 72-73 22.601257600471662 27.26415339180459 28.58252335903999 21.55206564868376 74-75 21.838796213167896 27.969941494802658 28.277837863727022 21.91342442830242 76-77 21.958515277439712 27.730579024593858 28.353612341993333 21.957293355973096 78-79 22.088001379831304 27.817819270438633 28.25321858843844 21.840960761291626 80-81 22.150660254427716 27.757802640431407 28.050076439712303 22.041460665428584 82-83 22.262226295917014 27.849805739479834 27.937506351576364 21.95046161302679 84-85 22.08670517689445 27.83895669627 27.91278180941395 22.161556317421603 86-87 22.188205702983996 27.66028343914501 27.772805046909248 22.378705810961744 88-89 22.008928375122697 27.84027032348626 27.696040470349576 22.45476083104147 90-91 21.948929117946534 27.82275480230847 27.798081226389098 22.4302348533559 92-93 22.030633837741018 27.487735080674124 27.789783606807276 22.69184747477758 94-95 21.939633375164938 27.219365522476092 28.15348514651984 22.687515955839128 96-97 21.920823228105395 27.10738898827834 27.99065473081041 22.981133052805852 98-99 21.900801810817995 27.42747150072714 27.48997247806846 23.1817542103864 100-101 21.819703683941263 27.29492003785919 27.724378472644684 23.160997805554864 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 350.0 1 260.5 2 197.5 3 243.0 4 233.5 5 241.0 6 407.5 7 822.5 8 1261.5 9 1455.0 10 1313.5 11 1104.5 12 1113.0 13 1210.5 14 1353.0 15 1469.0 16 1574.0 17 1766.0 18 1991.5 19 2216.0 20 2521.0 21 2923.0 22 3422.0 23 4120.0 24 4919.5 25 5852.5 26 7435.5 27 9462.0 28 11101.0 29 12490.5 30 14070.0 31 16545.0 32 19535.5 33 21396.5 34 22400.5 35 24047.0 36 26538.0 37 28414.5 38 29652.0 39 30341.5 40 30937.5 41 32291.5 42 33334.0 43 34249.0 44 35150.5 45 35875.5 46 36183.0 47 36487.5 48 36179.0 49 34968.0 50 34507.0 51 33960.0 52 33018.5 53 32450.5 54 31460.0 55 29322.5 56 26918.5 57 24289.0 58 21893.0 59 19540.5 60 16275.0 61 13389.0 62 10895.0 63 8279.0 64 6117.0 65 4677.5 66 3599.0 67 2714.5 68 2040.5 69 1427.5 70 998.5 71 704.0 72 482.0 73 346.5 74 258.0 75 185.0 76 129.5 77 89.5 78 61.0 79 38.0 80 22.5 81 13.0 82 7.5 83 4.5 84 1.0 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01758898069902518 2 0.012996302405390828 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4429139859757194E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9543311887805758E-4 26-27 2.931496783170863E-4 28-29 9.771655943902879E-5 30-31 4.397245174756295E-4 32-33 0.002296339146817176 34-35 0.0034689378600855215 36-37 0.08042072841832068 38-39 0.0753883256072107 40-41 0.08134903573299145 42-43 0.08164218541130853 44-45 0.07895498002673526 46-47 0.07485088453029604 48-49 0.07543718388693021 50-51 0.07499745936945458 52-53 0.0604376920130393 54-55 0.05105690230689253 56-57 0.02814236911844029 58-59 0.013436026922866457 60-61 0.009283073146707734 62-63 0.001661181510463489 64-65 0.0048369696922319246 66-67 0.18986327499003292 68-69 0.5094452826353765 70-71 0.47451161263592373 72-73 0.22440607875172958 74-75 0.028972959873672034 76-77 0.0380117416217822 78-79 0.005325552489427068 80-81 0.0012214569929878598 82-83 0.0 84-85 3.4200795803660076E-4 86-87 8.79449034951259E-4 88-89 1.4657483915854315E-4 90-91 5.862993566341726E-4 92-93 0.0021986225873781473 94-95 0.09918230783061421 96-97 0.01724697274098858 98-99 0.017979846936781296 100-101 0.010015947342500449 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1023368.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.3254624882718 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07363910133546 55.762229964681275 2 9.752163150763922 12.93633462470998 3 2.7232240774509395 5.418572891883926 4 1.1306053893848014 2.999517013707183 5 0.6051265887310331 2.006765043076801 6 0.3584509903223403 1.4264656627504957 7 0.24295943916639706 1.1280078018021695 8 0.1800324813404077 0.9552590070250965 9 0.13493301245327863 0.8054545010309502 >10 0.7163974654697643 8.499913127426518 >50 0.0521415509905907 2.4064174944760186 >100 0.027971277514766715 3.5217698849511745 >500 0.0017666063072514534 0.8433381311637768 >1k 5.888687690838178E-4 1.2899548513146526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4722 0.46141759367109386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4262 0.4164679763291407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3130 0.30585283104416006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 0.10719506570461457 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.885827971951439E-5 0.0 20-21 0.0 0.0 0.0 9.771655943902878E-5 0.0 22-23 0.0 0.0 0.0 1.9543311887805756E-4 0.0 24-25 0.0 0.0 0.0 1.9543311887805756E-4 0.0 26-27 0.0 0.0 0.0 2.9314967831708635E-4 9.771655943902878E-5 28-29 0.0 0.0 0.0 0.0014168901118659172 9.771655943902878E-5 30-31 0.0 0.0 0.0 0.005325552489427068 9.771655943902878E-5 32-33 0.0 0.0 0.0 0.015194924992768975 9.771655943902878E-5 34-35 0.0 0.0 0.0 0.03029213342609892 9.771655943902878E-5 36-37 0.0 0.0 0.0 0.06356462191508822 9.771655943902878E-5 38-39 0.0 0.0 0.0 0.11804160380234677 9.771655943902878E-5 40-41 0.0 0.0 0.0 0.17359346784343463 9.771655943902878E-5 42-43 0.0 0.0 0.0 0.23847726331094973 9.771655943902878E-5 44-45 0.0 0.0 0.0 0.3194354328061851 9.771655943902878E-5 46-47 0.0 0.0 0.0 0.41788486644100653 9.771655943902878E-5 48-49 0.0 0.0 0.0 0.526399105698048 9.771655943902878E-5 50-51 0.0 0.0 0.0 0.6464438989688949 9.771655943902878E-5 52-53 0.0 0.0 0.0 0.7586225092048999 9.771655943902878E-5 54-55 0.0 0.0 0.0 0.8836019887274178 9.771655943902878E-5 56-57 0.0 0.0 0.0 1.028564504655217 9.771655943902878E-5 58-59 0.0 0.0 0.0 1.1734781623032964 9.771655943902878E-5 60-61 0.0 0.0 0.0 1.3188804027485714 9.771655943902878E-5 62-63 0.0 0.0 0.0 1.458663941026102 9.771655943902878E-5 64-65 0.0 9.771655943902878E-5 0.0 1.6092451591216455 9.771655943902878E-5 66-67 0.0 9.771655943902878E-5 0.0 1.7764870506015433 1.4657483915854317E-4 68-69 0.0 9.771655943902878E-5 0.0 1.9750959576613691 1.9543311887805756E-4 70-71 0.0 9.771655943902878E-5 0.0 2.1980362880215134 1.9543311887805756E-4 72-73 0.0 9.771655943902878E-5 0.0 2.4464806403952437 2.9314967831708635E-4 74-75 0.0 9.771655943902878E-5 0.0 2.6935081026571086 3.908662377561151E-4 76-77 0.0 9.771655943902878E-5 0.0 2.9775701409463653 3.908662377561151E-4 78-79 0.0 9.771655943902878E-5 0.0 3.3067772296964533 4.885827971951439E-4 80-81 0.0 9.771655943902878E-5 0.0 3.6592897178727495 4.885827971951439E-4 82-83 0.0 9.771655943902878E-5 0.0 4.007356102594571 4.885827971951439E-4 84-85 0.0 9.771655943902878E-5 0.0 4.343745358463426 5.862993566341727E-4 86-87 0.0 9.771655943902878E-5 0.0 4.678033708304344 6.351576363536871E-4 88-89 0.0 9.771655943902878E-5 0.0 5.0342105674596045 6.840159160732014E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2280 0.0 61.391617 1 GTATCAA 4035 0.0 39.393368 1 TGGTATC 835 0.0 38.061234 2 GTGTACG 55 0.0025932693 34.507954 1 GTGGTAT 1110 0.0 31.63229 1 TAAGGTG 530 0.0 29.531816 5 TATCAAC 5675 0.0 28.251753 2 TAGCGCG 85 6.462271E-4 27.899933 4 ATCAACG 5770 0.0 27.701685 3 TCAACGC 5790 0.0 27.605999 4 TAACCCG 70 0.008451823 27.102793 5 CAACGCA 5980 0.0 26.808197 5 AACGCAG 6245 0.0 25.594671 6 GTAAGGT 520 0.0 25.539171 4 CGTATTG 100 0.0016677992 23.714943 5 CGTACAC 100 0.0016677992 23.714943 3 GTGTAGC 660 0.0 23.0053 1 ACGCAGA 7160 0.0 22.390087 7 GGTAAGG 595 0.0 22.319948 3 CGCAGAG 7215 0.0 22.15367 8 >>END_MODULE