FastQCFastQC Report
Fri 27 May 2016
SRR939861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310705
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286161.2384099225128262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192640.8336849576211589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141330.611631515057093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76700.33193332770734474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47260.20452632421706796No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35910.1554071160100489No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.15177186183437522No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34920.15112270930300492No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.15068994094875807No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.128618754882168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA259750.036.6934551
GGTATCA155100.035.3598371
TATCAAC328200.029.9795422
GTACATG231100.029.7052231
TACATGG236550.029.6820262
ATCAACG333950.029.5233733
TCAACGC336950.029.3309214
CAACGCA338550.029.0515255
AACGCAG344250.028.57056
ACATGGG235300.028.1693213
CGTCACT4550.028.1524771
ATGGGAG54700.026.194625
CATGGGA172500.026.1573244
ATGGGCG3650.025.9973815
ACGCAGA381400.025.6010137
CGCAGAG383000.025.3701848
AACGCGG2251.03682396E-1025.304126
ATGGGAT62450.024.7672425
TAAGGTG7350.024.5295015
CATGGGG55100.024.4550554
Produced by FastQC (version 0.11.5)