##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939861_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2310705 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.04990598107504 31.0 30.0 33.0 16.0 34.0 2 29.796498471245787 31.0 30.0 33.0 25.0 34.0 3 30.01591029577553 31.0 30.0 33.0 25.0 34.0 4 33.83891063549869 35.0 35.0 37.0 28.0 37.0 5 33.8634295593769 35.0 35.0 37.0 28.0 37.0 6 33.95154033076485 35.0 35.0 37.0 29.0 37.0 7 33.89509132494195 35.0 35.0 37.0 29.0 37.0 8 33.77585801735834 35.0 35.0 37.0 28.0 37.0 9 34.99295020350932 37.0 34.0 39.0 28.0 39.0 10-11 34.842841254076134 37.0 34.0 39.0 27.0 39.0 12-13 34.73908006430938 37.0 34.0 39.0 27.0 39.0 14-15 35.268560893753204 38.0 33.5 40.0 27.0 41.0 16-17 35.18631673017542 38.0 33.5 40.0 27.0 41.0 18-19 35.23351314858452 38.0 33.5 40.0 27.0 41.0 20-21 35.49489939217685 38.0 34.0 40.0 27.0 41.0 22-23 35.3808772647309 38.0 34.0 40.0 27.0 41.0 24-25 35.14103336427627 38.0 34.0 40.0 25.5 41.0 26-27 34.96269493509557 38.0 34.0 40.0 25.0 41.0 28-29 34.92615587017815 38.0 34.0 40.0 25.0 41.0 30-31 34.571800381268915 38.0 33.5 40.0 24.5 41.0 32-33 34.078747611659644 37.5 33.0 40.0 23.5 41.0 34-35 33.79880469380557 37.0 33.0 40.0 22.5 41.0 36-37 33.487216671968085 37.0 33.0 40.0 20.0 41.0 38-39 33.17569010323689 37.0 32.0 40.0 18.5 41.0 40-41 32.84305309418554 37.0 31.0 40.0 16.0 41.0 42-43 32.29517008878243 36.0 31.0 40.0 13.0 41.0 44-45 31.731939386464305 36.0 30.0 39.5 8.5 41.0 46-47 31.09403320631582 36.0 29.5 39.0 2.0 41.0 48-49 30.56325731757191 35.0 28.5 39.0 2.0 40.0 50-51 29.867076714682316 35.0 27.5 38.5 2.0 40.0 52-53 29.965362519231142 35.0 28.0 38.5 2.0 40.0 54-55 30.049422362439167 35.0 28.0 39.0 2.0 40.0 56-57 29.751184378793486 35.0 27.0 39.0 2.0 40.0 58-59 29.362865229442964 35.0 26.5 39.0 2.0 40.0 60-61 28.948898496346352 35.0 26.0 38.0 2.0 40.0 62-63 28.5147253760216 34.0 25.5 38.0 2.0 40.0 64-65 27.978146063647245 34.0 24.0 38.0 2.0 40.0 66-67 27.382722805377583 33.5 22.0 37.0 2.0 39.5 68-69 26.83521847228443 33.0 21.5 36.5 2.0 39.0 70-71 26.252801850517482 32.5 19.5 36.0 2.0 39.0 72-73 25.59675250626973 32.0 17.5 35.5 2.0 38.5 74-75 25.247177376601513 32.0 14.5 35.0 2.0 38.0 76-77 24.678087207151066 31.0 7.5 35.0 2.0 37.0 78-79 23.998970444085245 31.0 4.5 35.0 2.0 36.5 80-81 23.23540153329828 30.5 2.0 34.0 2.0 36.0 82-83 22.468256873984345 29.5 2.0 34.0 2.0 35.5 84-85 21.6816168658483 29.0 2.0 34.0 2.0 35.0 86-87 20.75512733126903 28.5 2.0 34.0 2.0 35.0 88-89 19.754403742580728 26.5 2.0 33.5 2.0 35.0 90-91 18.379291385096757 23.5 2.0 32.5 2.0 34.5 92-93 16.85744329111678 18.5 2.0 31.5 2.0 34.0 94-95 16.348170579974507 16.5 2.0 31.0 2.0 34.0 96-97 16.052522498544818 8.5 2.0 31.0 2.0 34.0 98-99 15.247894906532855 2.0 2.0 31.0 2.0 34.0 100-101 13.692235270188103 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.712836041667007 1101 2 1.04428636235275 1101 3 1.0376106960345304 1101 4 0.9813848983519549 1101 5 1.0452362144381269 1101 6 0.944879668386065 1101 7 0.8854229042039918 1101 8 0.9807864330853775 1101 9 1.0805058630295434 1101 10-11 1.173648835520261 1101 12-13 1.0261073409936543 1101 14-15 1.4618941387403979 1101 16-17 1.3786741824493305 1101 18-19 1.2650478064579431 1101 20-21 1.1945137514403115 1101 22-23 1.334465659511224 1101 24-25 1.3794729827445877 1101 26-27 1.3919920096381446 1101 28-29 1.3034657549920183 1101 30-31 1.583659680978215 1101 32-33 1.8132837517954528 1101 34-35 1.6076432838902832 1101 36-37 1.693325227243669 1101 38-39 1.5461518431744778 1101 40-41 1.4663485632369841 1101 42-43 1.4711597127726108 1101 44-45 1.4924522624663084 1101 46-47 1.397397120559777 1101 48-49 1.2662135264473555 1101 50-51 1.1357768628646312 1101 52-53 1.2103700434710838 1101 54-55 1.153327083415359 1101 56-57 1.3661584049786697 1101 58-59 1.3424459231619075 1101 60-61 1.2883855205914791 1101 62-63 0.9309214247236568 1101 64-65 1.0800560581516265 1101 66-67 1.1233409882699732 1101 68-69 0.841270743274606 1101 70-71 0.9642947773795321 1101 72-73 0.7828447959004059 1101 74-75 0.7050142968725481 1101 76-77 0.4664807308923571 1101 78-79 0.30848727852447055 1101 80-81 0.09689828687941926 1101 82-83 0.07927032215876295 1101 84-85 0.5134111914515351 1101 86-87 -0.023740604374903995 1101 88-89 -3.681491153284437 1101 90-91 -7.245501617399588 1101 92-93 -9.592251648618015 1101 94-95 -7.166978315433655 1101 96-97 -3.9874309138900106 1101 98-99 -2.861666953640974 1101 100-101 -2.0260191439860336 1102 1 1.8138998714542396 1102 2 1.2206731404682536 1102 3 0.9810841847207854 1102 4 1.005036114157427 1102 5 0.9251965318984432 1102 6 0.96123816213818 1102 7 0.9223086691482649 1102 8 1.145542849836474 1102 9 1.2514890612733751 1102 10-11 1.243985081720865 1102 12-13 1.167107003269912 1102 14-15 1.2427272609963893 1102 16-17 1.1715914823480205 1102 18-19 1.3070785815339292 1102 20-21 1.3680034930816447 1102 22-23 1.2215709448877874 1102 24-25 1.2109541124305068 1102 26-27 1.2900258242278113 1102 28-29 1.152302718855573 1102 30-31 1.249687838695202 1102 32-33 1.2443722776313138 1102 34-35 1.3452688749068287 1102 36-37 1.1452470864128728 1102 38-39 1.0638612318945135 1102 40-41 1.022769535881359 1102 42-43 1.1725802632623115 1102 44-45 1.2865041030607003 1102 46-47 1.1831958372095563 1102 48-49 0.7898541883858918 1102 50-51 0.9247206318615895 1102 52-53 1.146260578763215 1102 54-55 1.0742786200583865 1102 56-57 1.1952965762046084 1102 58-59 0.8827986333949376 1102 60-61 0.8757240464273437 1102 62-63 0.9335018451897135 1102 64-65 0.8740973448790825 1102 66-67 0.9160947032311348 1102 68-69 0.8200627476650162 1102 70-71 0.8302131748803774 1102 72-73 0.7463073085650471 1102 74-75 0.5406642039985208 1102 76-77 0.6796688430166391 1102 78-79 0.8197957469473032 1102 80-81 0.7002048133907337 1102 82-83 0.5157202123985485 1102 84-85 0.5536953015494746 1102 86-87 0.5368280379756563 1102 88-89 0.11890883827247123 1102 90-91 -5.160696019628563 1102 92-93 -9.31991966617965 1102 94-95 -7.085581616683232 1102 96-97 -3.5393190006850137 1102 98-99 -2.1369170802873754 1102 100-101 -1.4569810234186598 1103 1 1.9475512297983535 1103 2 1.1492695952209182 1103 3 0.9109941931398495 1103 4 0.8636439654480057 1103 5 0.8505267491513635 1103 6 0.8163083381171532 1103 7 0.8438150142477525 1103 8 0.8202531878310921 1103 9 0.7822590719512164 1103 10-11 0.9250004131445095 1103 12-13 0.8859102641171503 1103 14-15 0.907360390013416 1103 16-17 0.9045679038351153 1103 18-19 0.8374959797351238 1103 20-21 0.7125475930631637 1103 22-23 0.6589576853752703 1103 24-25 0.6373328625075132 1103 26-27 0.6907527385381655 1103 28-29 0.6211101507330312 1103 30-31 0.8034838612008031 1103 32-33 0.9735639704792192 1103 34-35 0.8072249083982754 1103 36-37 0.9336170349744393 1103 38-39 1.0172830167360871 1103 40-41 0.7309594499453098 1103 42-43 1.003609904528048 1103 44-45 0.7350919847420485 1103 46-47 0.8895768075580328 1103 48-49 0.7223996461981663 1103 50-51 0.7565928212289315 1103 52-53 0.7027462740546966 1103 54-55 0.5739304994604275 1103 56-57 0.5129760331749758 1103 58-59 0.5823776160229208 1103 60-61 0.7082940812329888 1103 62-63 0.6767197500748239 1103 64-65 0.4895575571011008 1103 66-67 0.6280802547824997 1103 68-69 0.6890128287755068 1103 70-71 0.6037432396661053 1103 72-73 0.6467858861539071 1103 74-75 0.5372853486832163 1103 76-77 0.6357049012519198 1103 78-79 0.3625622821944319 1103 80-81 0.19347501657765775 1103 82-83 -0.08367864589423135 1103 84-85 0.00260659849602618 1103 86-87 -4.734851562382403E-4 1103 88-89 -0.3780520849497755 1103 90-91 -5.751721053510163 1103 92-93 -8.997180749569452 1103 94-95 -7.543479558632759 1103 96-97 -4.477635560059948 1103 98-99 -3.0851119225839323 1103 100-101 -2.317029805221159 1104 1 2.0037185898353513 1104 2 1.2802596858789954 1104 3 1.0135646445832016 1104 4 0.9378158660244438 1104 5 0.7746312397332389 1104 6 0.8008634866918314 1104 7 0.8832869474747724 1104 8 0.9566484401895323 1104 9 1.026231258602003 1104 10-11 1.0666671161703931 1104 12-13 0.8611733594357602 1104 14-15 1.0825140258990942 1104 16-17 0.9052525479547029 1104 18-19 1.044788621283388 1104 20-21 0.8658993314338517 1104 22-23 1.0785460880929065 1104 24-25 1.0158630701113864 1104 26-27 0.9720903869837656 1104 28-29 1.0356913434172554 1104 30-31 1.1322479313101041 1104 32-33 1.1729211212834514 1104 34-35 1.170993181633456 1104 36-37 1.1582798395647202 1104 38-39 1.1808527868076482 1104 40-41 1.051998185604326 1104 42-43 1.2551791684792661 1104 44-45 1.0464858009112845 1104 46-47 1.1157417933379357 1104 48-49 0.89128640686069 1104 50-51 0.830308140895557 1104 52-53 0.8183070568541702 1104 54-55 0.8202764887111904 1104 56-57 0.9884551022625843 1104 58-59 0.8583630054338052 1104 60-61 0.8350749558467605 1104 62-63 0.7406592570583399 1104 64-65 0.8324269140592016 1104 66-67 0.7616820282398322 1104 68-69 0.678975593297924 1104 70-71 0.6112519640610294 1104 72-73 0.7005628092476108 1104 74-75 0.5328920640857824 1104 76-77 0.6715550016397316 1104 78-79 0.5657287885169389 1104 80-81 0.25755587003036595 1104 82-83 0.192983388607761 1104 84-85 0.18839594262659531 1104 86-87 0.3465563664908693 1104 88-89 -0.051445025588982674 1104 90-91 -2.6601978966863022 1104 92-93 -7.031393139773696 1104 94-95 -4.728117133560021 1104 96-97 -2.303000861147016 1104 98-99 -1.6320174075177611 1104 100-101 -1.032383285925686 1105 1 1.8971713848073541 1105 2 1.1338116525496424 1105 3 0.8407204477324193 1105 4 0.6723607220421215 1105 5 0.5576222890460087 1105 6 0.5875304941055859 1105 7 0.660695344293103 1105 8 0.5316689743713496 1105 9 0.5888774909281267 1105 10-11 0.6236282038064971 1105 12-13 0.5697022649156125 1105 14-15 0.7431084842077382 1105 16-17 0.6028167543559348 1105 18-19 0.683820580262207 1105 20-21 0.6717405108857974 1105 22-23 0.48840290640632844 1105 24-25 0.6152611987665253 1105 26-27 0.6085930137148807 1105 28-29 0.6475521477567128 1105 30-31 0.7629496963561735 1105 32-33 0.7507441513066055 1105 34-35 0.678607359905179 1105 36-37 0.8845676848302801 1105 38-39 0.6020806987119016 1105 40-41 0.2991379653006092 1105 42-43 0.5665354467204651 1105 44-45 0.2618305714324194 1105 46-47 0.49473836346833266 1105 48-49 0.33080403766289024 1105 50-51 0.2626683851468812 1105 52-53 0.15580367023323305 1105 54-55 0.2293753830464702 1105 56-57 0.2953146806273885 1105 58-59 0.2646981847585117 1105 60-61 0.35384639699961795 1105 62-63 0.19470450874771927 1105 64-65 0.13334605549092515 1105 66-67 0.17710675518346974 1105 68-69 0.28153418094653304 1105 70-71 -0.016799300980391507 1105 72-73 0.01854042287902047 1105 74-75 0.12867892892904464 1105 76-77 -0.011207705426677705 1105 78-79 -0.0849455511545294 1105 80-81 -0.28214631820928204 1105 82-83 -0.5185751575793169 1105 84-85 -0.6382454336961558 1105 86-87 -0.37612280630864703 1105 88-89 -0.6449506125306002 1105 90-91 -3.3894895585574893 1105 92-93 -7.420653852039948 1105 94-95 -5.280743614469488 1105 96-97 -2.9890983622318075 1105 98-99 -2.1666414514898626 1105 100-101 -1.5157607853234207 1106 1 1.6496705802501417 1106 2 1.164772353595005 1106 3 0.8879920304095883 1106 4 0.7763739511709673 1106 5 0.8386553006891972 1106 6 0.869950529806566 1106 7 0.7415997715841129 1106 8 -0.15984623221251582 1106 9 -0.4750669788768107 1106 10-11 -0.457797963905584 1106 12-13 -0.29869688162022356 1106 14-15 0.6489027079756369 1106 16-17 0.803798133155226 1106 18-19 0.8028945050425307 1106 20-21 0.7080421505172936 1106 22-23 0.6573106887642766 1106 24-25 0.5777892858679436 1106 26-27 0.7763927877510497 1106 28-29 0.5538510071859974 1106 30-31 0.571115562930359 1106 32-33 0.6979337940397556 1106 34-35 0.7979016555311844 1106 36-37 0.6150029167979696 1106 38-39 0.41345474849272534 1106 40-41 0.4023424741514603 1106 42-43 0.5043640798347724 1106 44-45 0.5076448828853017 1106 46-47 0.45299750075855627 1106 48-49 0.3120366772368257 1106 50-51 0.4168099633243223 1106 52-53 0.5304740189784702 1106 54-55 0.4312903740198628 1106 56-57 0.42573593918293184 1106 58-59 0.357651602977306 1106 60-61 0.24008562688360868 1106 62-63 0.3690105170615361 1106 64-65 0.25103847679726243 1106 66-67 0.2595013780986335 1106 68-69 0.2558353202864332 1106 70-71 0.3902315335617388 1106 72-73 0.466633088321867 1106 74-75 0.32593011259651306 1106 76-77 0.28337209488385184 1106 78-79 0.43425920241203997 1106 80-81 0.33005393671498595 1106 82-83 0.1426484246171107 1106 84-85 0.1816820819433893 1106 86-87 0.29986698006829116 1106 88-89 0.03950648239701238 1106 90-91 -3.21501530818942 1106 92-93 -7.750189717553459 1106 94-95 -5.454743818640587 1106 96-97 -2.8375249415441335 1106 98-99 -1.5954721664977782 1106 100-101 -1.0771551979133918 1107 1 1.7616179993661412 1107 2 0.9906763927221398 1107 3 0.8547618535858739 1107 4 0.837066655090446 1107 5 0.703339242815936 1107 6 0.6513903877891352 1107 7 0.5695336646102405 1107 8 0.7325324476662871 1107 9 0.739947971944801 1107 10-11 0.6528132492648879 1107 12-13 0.6775848072674151 1107 14-15 0.8109949693927518 1107 16-17 0.8040924655875727 1107 18-19 0.8763052636298951 1107 20-21 0.6738448814158886 1107 22-23 0.5657214889470055 1107 24-25 0.5160514531456997 1107 26-27 0.5754073211722073 1107 28-29 0.6086453072462916 1107 30-31 0.6387015233728022 1107 32-33 0.8090410771714645 1107 34-35 0.8348279423923586 1107 36-37 0.6287598609636049 1107 38-39 0.5064463451435373 1107 40-41 0.4662304256375194 1107 42-43 0.6495812817976443 1107 44-45 0.2990183474285004 1107 46-47 0.3706885889693048 1107 48-49 0.40428751466006574 1107 50-51 0.37725096101993927 1107 52-53 0.2303619104225163 1107 54-55 0.10792211504638516 1107 56-57 0.07727663156855513 1107 58-59 0.05146865063944617 1107 60-61 -0.15071658859413617 1107 62-63 -0.08615727322258238 1107 64-65 0.05353761685826797 1107 66-67 0.06622561275292682 1107 68-69 0.2981491113930552 1107 70-71 0.24071789190908177 1107 72-73 -0.0550915387756703 1107 74-75 -0.15695339397247565 1107 76-77 0.03751761573199275 1107 78-79 -0.11075602691768793 1107 80-81 0.27462060522631404 1107 82-83 0.15912443982437452 1107 84-85 0.12137684790796044 1107 86-87 0.2920552797618221 1107 88-89 0.3103027189929044 1107 90-91 -2.5183541321111687 1107 92-93 -4.280682105420489 1107 94-95 -2.8433911228448654 1107 96-97 -1.3839328018386805 1107 98-99 -0.9931570588073733 1107 100-101 -0.5384811822496616 1108 1 1.5279945424271908 1108 2 0.7617492550077287 1108 3 -0.7026974796069005 1108 4 -0.2766212046037495 1108 5 0.26461701782932323 1108 6 0.4731257021090869 1108 7 0.3535775331734854 1108 8 0.24062374486437932 1108 9 0.7208786758865458 1108 10-11 0.34262200570725554 1108 12-13 0.4023345891232495 1108 14-15 0.30609576712579667 1108 16-17 0.26248784795926383 1108 18-19 0.42117379319549997 1108 20-21 0.29674523856869683 1108 22-23 0.05739815442185403 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0.4362575404478015 2311 76-77 0.47920328342867435 2311 78-79 0.4498293808781675 2311 80-81 0.6871344341612904 2311 82-83 0.9037980378320292 2311 84-85 0.949206729754323 2311 86-87 1.1873610716279153 2311 88-89 1.4494286520486348 2311 90-91 2.0826865961198138 2311 92-93 2.9204964964362823 2311 94-95 2.576998229535471 2311 96-97 1.9165439112619058 2311 98-99 1.6730620932527103 2311 100-101 1.7538422059885779 2312 1 1.349804126773389 2312 2 0.6523790675199237 2312 3 0.03476115196158247 2312 4 -0.3109783235325452 2312 5 -0.0021612019752481615 2312 6 -0.17024191216104612 2312 7 0.09600421119487379 2312 8 0.12288369752306494 2312 9 0.15174446485325177 2312 10-11 0.21012299965399706 2312 12-13 0.30935202184470967 2312 14-15 0.262093606825502 2312 16-17 0.07011605175024727 2312 18-19 -0.40298030904357773 2312 20-21 0.24352590949501973 2312 22-23 0.5110898996328075 2312 24-25 0.4291975268175392 2312 26-27 0.5972066752916376 2312 28-29 0.552373429764053 2312 30-31 0.4044708511909789 2312 32-33 0.5305614873577582 2312 34-35 0.4343910042550263 2312 36-37 0.6008100296752801 2312 38-39 0.48460169119879737 2312 40-41 0.2892493991032481 2312 42-43 0.47923342097930544 2312 44-45 0.19068554514107916 2312 46-47 0.3098686251190479 2312 48-49 0.2809361708242548 2312 50-51 0.1864037625606798 2312 52-53 0.23791563617625044 2312 54-55 0.27457518371931044 2312 56-57 0.352575578230951 2312 58-59 0.3996267742257409 2312 60-61 0.41386044460363536 2312 62-63 0.429924084298122 2312 64-65 0.5454340976653036 2312 66-67 0.6538786022517336 2312 68-69 0.6319717311165505 2312 70-71 0.566973348808105 2312 72-73 0.61880604210101 2312 74-75 0.604995299912062 2312 76-77 0.879664621469864 2312 78-79 0.8912665338436234 2312 80-81 1.015781265602822 2312 82-83 1.1777220698791275 2312 84-85 1.3443382431232678 2312 86-87 1.8313600795156724 2312 88-89 2.176174120271792 2312 90-91 2.7501290816885593 2312 92-93 3.460559901533962 2312 94-95 3.192444176967564 2312 96-97 2.4981313961403853 2312 98-99 2.1172464202192103 2312 100-101 1.9424459016066713 2313 1 -7.62378760511119 2313 2 -2.8789823713427722 2313 3 -1.5532162152292202 2313 4 -0.6988094988909452 2313 5 -0.641549031156174 2313 6 -0.14997449877920843 2313 7 -0.2226341504992817 2313 8 -0.10734350811878102 2313 9 0.022068520091025334 2313 10-11 -0.2321995358269433 2313 12-13 -0.23965465152324583 2313 14-15 -0.31458979312184 2313 16-17 -0.7015923384243834 2313 18-19 -0.604789856786276 2313 20-21 -0.35707969964425956 2313 22-23 -0.8683377384017987 2313 24-25 -0.3172728279519461 2313 26-27 -0.3721414048685574 2313 28-29 -0.7025767493359041 2313 30-31 -0.19951382359700887 2313 32-33 -0.32090471899410034 2313 34-35 -0.38893427157486116 2313 36-37 -0.11569099945139527 2313 38-39 -0.3349561034385786 2313 40-41 -0.45908624777067786 2313 42-43 -0.47190948801877397 2313 44-45 -0.48816859410215585 2313 46-47 -1.4774227573411203 2313 48-49 -0.8712389562210348 2313 50-51 -1.0273439538152012 2313 52-53 -1.4049654398594562 2313 54-55 -0.9247976672806963 2313 56-57 -0.7496146067725213 2313 58-59 -0.70586020612512 2313 60-61 -0.5333205261260865 2313 62-63 -0.6765586160026373 2313 64-65 -0.7415581200627983 2313 66-67 -0.5365108304521549 2313 68-69 -0.553640539424098 2313 70-71 -0.37555212008275163 2313 72-73 -0.3255313678504024 2313 74-75 -0.27671995110709346 2313 76-77 -0.19897132696211628 2313 78-79 -0.35867413181785324 2313 80-81 -0.16793081028130885 2313 82-83 0.12653448561439617 2313 84-85 0.046645401536977715 2313 86-87 0.5974591309640296 2313 88-89 0.9714272734680698 2313 90-91 1.681794372197455 2313 92-93 2.5421835084235234 2313 94-95 2.1730403524456268 2313 96-97 1.5274246094887172 2313 98-99 1.1150924341483197 2313 100-101 1.0779774973912417 2314 1 0.6542197049137393 2314 2 0.10396975017950894 2314 3 -1.0773439240952882 2314 4 -0.13679704794920156 2314 5 0.006463504369996542 2314 6 -3.5697711441833917 2314 7 -2.699422252592118 2314 8 -2.538251368708881 2314 9 -3.2953568376968576 2314 10-11 -2.2362568210584115 2314 12-13 -0.8626173517761728 2314 14-15 -0.9806353460953972 2314 16-17 -1.8913196870402516 2314 18-19 -2.740168160652722 2314 20-21 -2.665586792957072 2314 22-23 -3.7663442423200735 2314 24-25 -2.750897253778355 2314 26-27 -1.7011064348832576 2314 28-29 -1.6226240967482823 2314 30-31 -1.737340236476328 2314 32-33 -1.8992655238178031 2314 34-35 -1.40905103215011 2314 36-37 -1.6508833027223488 2314 38-39 -3.0389746728433664 2314 40-41 -2.3910589355147636 2314 42-43 -1.700657153076918 2314 44-45 -2.1319059969116623 2314 46-47 -1.392273271390227 2314 48-49 -1.9811867948759492 2314 50-51 -2.9199891388748647 2314 52-53 -1.7434095319633904 2314 54-55 -2.1153596485091946 2314 56-57 -1.2621147238566728 2314 58-59 -0.641828159979486 2314 60-61 -0.9877164613048954 2314 62-63 -0.7059296370928294 2314 64-65 -1.282635356717833 2314 66-67 -0.7483619749107255 2314 68-69 -0.6569628342053839 2314 70-71 -0.6067008618041889 2314 72-73 -1.5735588191880971 2314 74-75 -1.547787718847328 2314 76-77 -0.7311250480951941 2314 78-79 -1.7601168072744855 2314 80-81 -0.6707614083868982 2314 82-83 -0.27977952925941096 2314 84-85 -1.5309398804314682 2314 86-87 -0.24972717362450325 2314 88-89 0.7587079322729267 2314 90-91 1.5354650100747627 2314 92-93 2.4560384523693095 2314 94-95 2.3178091602053694 2314 96-97 1.5379145690613303 2314 98-99 1.2518582207111457 2314 100-101 1.3429771837476299 2315 1 -2.5065452529042282 2315 2 -1.6143069085983548 2315 3 -2.296191098994491 2315 4 -3.675151481014872 2315 5 -3.6195264188030905 2315 6 -2.771226244206016 2315 7 -5.505213514221726 2315 8 -2.9808602561486524 2315 9 -2.4735250774917503 2315 10-11 -3.9980407134965503 2315 12-13 -2.904506429575264 2315 14-15 -1.6596316411774552 2315 16-17 -2.135856748622224 2315 18-19 -1.6984528676229118 2315 20-21 -1.1662964265506375 2315 22-23 -0.7221071275716184 2315 24-25 -2.2226291363052297 2315 26-27 -3.8592607360338818 2315 28-29 -3.146659217708759 2315 30-31 -3.213933236132938 2315 32-33 -4.074918442012244 2315 34-35 -3.60512687343725 2315 36-37 -2.6776974857112847 2315 38-39 -1.7571635426685575 2315 40-41 -1.4535422556137583 2315 42-43 -2.841598842384421 2315 44-45 -5.955646924016797 2315 46-47 -2.7141807168796355 2315 48-49 -1.5943753703279775 2315 50-51 -2.9479264974599353 2315 52-53 -2.8225680460871736 2315 54-55 -1.4800014113053912 2315 56-57 -1.0803432518742753 2315 58-59 -2.205431667114528 2315 60-61 -2.6555374094973097 2315 62-63 -4.136119040077144 2315 64-65 -3.755801972230312 2315 66-67 -3.220944102783445 2315 68-69 -1.705112870680761 2315 70-71 -3.5148908762479003 2315 72-73 -2.3498055493297905 2315 74-75 -1.3669304473967152 2315 76-77 -2.7885381265720923 2315 78-79 -1.4884798563588966 2315 80-81 -1.348343143263964 2315 82-83 -1.2999643388268645 2315 84-85 -2.5284017710603273 2315 86-87 -2.10610459648753 2315 88-89 -1.6136750230125365 2315 90-91 -0.969507505632361 2315 92-93 -0.39827858245309145 2315 94-95 -0.452570204624001 2315 96-97 -0.7134401452278407 2315 98-99 -0.971059671887776 2315 100-101 -1.006060260818316 2316 1 -7.069735959927492 2316 2 -5.822920731506766 2316 3 -3.16380587512829 2316 4 -4.04880365945694 2316 5 -1.8220305693245606 2316 6 -2.6769188766336143 2316 7 -3.1754177164879565 2316 8 -2.4149648915114312 2316 9 -7.263286179423993 2316 10-11 -6.722801120464929 2316 12-13 -5.983036114086325 2316 14-15 -5.001556176422291 2316 16-17 -4.7675917729213 2316 18-19 -3.9484712818540473 2316 20-21 -2.8132147934132803 2316 22-23 -3.5598050959657073 2316 24-25 -3.2592595878036867 2316 26-27 -4.292917189043074 2316 28-29 -4.491574948331518 2316 30-31 -7.243985723625901 2316 32-33 -5.199641188410837 2316 34-35 -5.920559416792361 2316 36-37 -4.3434458292779965 2316 38-39 -6.699425913564767 2316 40-41 -4.248173574969535 2316 42-43 -4.960697774728839 2316 44-45 -3.5095455326405727 2316 46-47 -4.562921291335847 2316 48-49 -5.4153159261079935 2316 50-51 -4.703279953618502 2316 52-53 -6.292479500601868 2316 54-55 -5.402647772083167 2316 56-57 -4.541810664232553 2316 58-59 -3.257021331476256 2316 60-61 -3.5415402978349526 2316 62-63 -4.3846821907197295 2316 64-65 -3.1595555630053838 2316 66-67 -4.1251857092070665 2316 68-69 -3.3682016291239556 2316 70-71 -2.890118434539346 2316 72-73 -2.480883362785459 2316 74-75 -2.5274913710730367 2316 76-77 -3.3525976154793256 2316 78-79 -5.206437013916606 2316 80-81 -5.850565255565812 2316 82-83 -3.631007604411238 2316 84-85 -2.4051178001939526 2316 86-87 -2.499386345318648 2316 88-89 -1.4613669879049986 2316 90-91 -1.2192318314942945 2316 92-93 -1.244506577446888 2316 94-95 -1.7248079697927814 2316 96-97 -3.1756586116660177 2316 98-99 -4.384514172402911 2316 100-101 -3.9369161327082765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17861.0 3 5101.0 4 4791.0 5 5141.0 6 6173.0 7 7893.0 8 10165.0 9 12956.0 10 16075.0 11 20593.0 12 26612.0 13 31517.0 14 35734.0 15 42067.0 16 48082.0 17 51370.0 18 41112.0 19 34889.0 20 36248.0 21 38958.0 22 42568.0 23 47558.0 24 52772.0 25 59895.0 26 67260.0 27 75532.0 28 85194.0 29 95818.0 30 107656.0 31 120877.0 32 134239.0 33 149104.0 34 168398.0 35 190086.0 36 200169.0 37 166455.0 38 52841.0 39 945.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.714450075510214 20.319894610716165 13.810502960823525 19.155152352950097 2 15.546120543234867 22.291408140203956 40.623548496675845 21.538922819885336 3 18.93583582165221 30.062575207461013 30.34362416223709 20.657964808649687 4 11.586107851277633 17.811330468531967 38.61497138954423 31.987590290646175 5 11.565471535010087 40.777481925700926 32.593057995913924 15.063988543375068 6 27.3446729726942 38.08887147647895 18.995528342886566 15.570927207940292 7 26.003086553550837 36.99962718594751 21.06113283451391 15.93615342598774 8 26.914811353631535 33.35926812733738 20.379026539313102 19.34689397971798 9 29.467589210896953 16.050526940028412 20.82135972245643 33.6605241266182 10-11 24.412762332868795 28.3125085340136 26.61946860458312 20.65526052853449 12-13 26.946631272878264 26.81419713074529 27.607154954447726 18.632016641928722 14-15 23.532825821138577 26.56775625891011 28.51097777224888 21.388440147702433 16-17 19.772438354905667 30.496025700427225 28.648395738729082 21.083140205938022 18-19 19.571649095063524 27.495820052218463 33.91926295935534 19.013267893362674 20-21 21.59138546854889 26.097852913265534 33.715118565123355 18.595643053062222 22-23 20.904585570118357 26.822512707338536 33.429461423941795 18.843440298601312 24-25 19.97102056537875 27.08392975187254 34.0328997478993 18.912149934849406 26-27 19.7534527546553 27.224291532400102 34.07808977942975 18.944165933514842 28-29 19.37282302516295 27.44104775457768 34.119674158963 19.066455061296367 30-31 19.463878487799008 27.504855348039808 33.84783214538609 19.1834340187751 32-33 19.144293518926176 27.74974462187772 33.45282065407163 19.65314120512448 34-35 19.716389909984773 27.528234256009814 33.69820990945998 19.05716592454543 36-37 19.94150937053716 27.2651130612807 33.206347003195624 19.587030564986517 38-39 19.57308700807447 27.51636535220528 33.324211353525435 19.586336286194815 40-41 19.46332171421657 27.54305340326489 33.454425434892826 19.539199447625712 42-43 19.276205799634404 27.771761639492855 32.862368729766004 20.089663831106737 44-45 19.369084174871 27.756345736789772 32.662336507809854 20.212233580529375 46-47 19.708997147222203 28.090395431218347 31.936798665017097 20.26380875654236 48-49 19.598225316894528 28.370480175828217 31.235498695194426 20.795795812082833 50-51 19.619973055119853 28.54925033789574 31.109838352413867 20.72093825457054 52-53 19.752387093079058 28.920729331457736 30.531577837501505 20.7953057379617 54-55 19.074745479726893 28.663100374534434 30.836784210544582 21.425369935194087 56-57 19.520609372441385 28.74884099730081 30.308340806980365 21.422208823277437 58-59 19.693167850858423 28.173736210848094 30.533516360889333 21.599579577404153 60-61 19.593790573890992 28.447430981561837 30.668375847471225 21.290402597075946 62-63 19.72330019940199 28.26367633785466 30.6134630273762 21.399560435367153 64-65 19.326398863888905 28.428209811967108 30.634173311145922 21.611218012998066 66-67 19.382784792999637 28.488797183618985 30.11234473780301 22.016073285578365 68-69 19.689597772018267 28.173608763614546 30.129857908487352 22.00693555587984 70-71 19.414667487495123 28.421647926609435 30.10230069774033 22.061383888155113 72-73 19.482674773882515 28.2202301238077 29.851022100058692 22.446073002251087 74-75 19.01238156387742 28.53885398951862 29.86473119772325 22.584033248880715 76-77 19.10699583567005 28.08090450244447 30.1179875406372 22.69411212124828 78-79 19.137386547470054 28.002374213232724 30.062607291377187 22.797631947920035 80-81 19.158310824107787 28.22749097334672 29.784430002184926 22.82976820036057 82-83 19.17307727774228 27.969003416220158 29.649276019523118 23.208643286514448 84-85 18.97864258195282 27.682692268063263 29.645724861341314 23.692940288642603 86-87 18.9961993154386 27.541503080613083 29.80085072340123 23.661446880547093 88-89 19.28422971654103 27.19632487548817 29.582435518394167 23.937009889576625 90-91 19.35083229526423 27.150411214449576 29.747503635777267 23.751252854508927 92-93 19.930565576483968 26.886370398326193 29.52963194880196 23.65343207638788 94-95 19.280184948528188 26.52382137216766 30.0437793731499 24.152214306154253 96-97 19.4543861746814 26.287687644808504 29.626141514734144 24.631784665775942 98-99 19.53017259811931 26.330617948198643 29.25172460276991 24.887484850912138 100-101 19.69859412534066 26.122797784366547 29.77457197595388 24.404036114338915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7255.0 1 4905.0 2 2901.0 3 3340.5 4 3070.5 5 3049.5 6 4538.5 7 7960.0 8 11999.5 9 13086.0 10 10989.5 11 9110.5 12 8491.5 13 8320.5 14 8273.5 15 8159.5 16 8134.0 17 8315.0 18 8598.0 19 9039.5 20 9642.0 21 10518.0 22 11779.5 23 13029.5 24 14449.0 25 16362.0 26 19121.5 27 22673.0 28 25519.5 29 29198.0 30 34341.5 31 38950.5 32 44115.5 33 49645.5 34 55087.0 35 59839.5 36 64785.0 37 70110.5 38 71959.0 39 71687.0 40 71416.0 41 72027.0 42 73024.0 43 74170.5 44 75581.5 45 76886.5 46 77697.0 47 78583.5 48 82999.5 49 82297.5 50 73729.5 51 66234.5 52 60967.0 53 57756.5 54 55107.0 55 51261.0 56 47456.0 57 43716.0 58 39839.0 59 35457.5 60 30963.0 61 26957.5 62 21953.5 63 15535.5 64 10243.0 65 7334.0 66 5423.5 67 4015.0 68 2934.0 69 2121.0 70 1642.5 71 1348.0 72 1136.5 73 1029.0 74 878.5 75 717.5 76 557.5 77 360.5 78 214.5 79 129.0 80 94.5 81 63.5 82 35.0 83 29.0 84 20.5 85 10.0 86 8.0 87 5.5 88 1.5 89 2.5 90 2.5 91 1.5 92 2.5 93 2.0 94 1.0 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13285988475378727 2 0.11914112792416166 3 0.12645491311093368 4 0.136624969435735 5 0.12420451766884998 6 0.14207785069924547 7 0.16986157904189414 8 0.15739785043958446 9 0.16860655081457823 10-11 0.1627209011968209 12-13 0.15235609911260847 14-15 0.1572680199333104 16-17 0.15172858499895053 18-19 0.16696203106844015 20-21 0.17302078802789625 22-23 0.20573807560895918 24-25 0.22837186053607017 26-27 0.24187423318857232 28-29 0.23674592819074697 30-31 0.22752796224528876 32-33 0.22936722775083795 34-35 0.21809361212270714 36-37 0.22536412047405446 38-39 0.21287875345403243 40-41 0.21733626750277515 42-43 0.21075818851822278 44-45 0.2037473411794236 46-47 0.2188725951603515 48-49 0.2577351933717199 50-51 0.2596610125481184 52-53 0.26048327242118746 54-55 0.2586656453333506 56-57 0.23293756667337456 58-59 0.23393293388814235 60-61 0.20456960105249264 62-63 0.19732073111885765 64-65 0.1987488666878723 66-67 0.2063006744694801 68-69 0.20606265187464431 70-71 0.2122512393403745 72-73 0.24226372470739452 74-75 0.2451632726808485 76-77 0.260201972990927 78-79 0.2571293176757743 80-81 0.2717568880493183 82-83 0.2708047976699752 84-85 0.24557440261738303 86-87 0.247781521224042 88-89 0.2511571143871676 90-91 0.4309940039944519 92-93 0.5811862613358261 94-95 0.33729965530000583 96-97 0.28495632285384764 98-99 0.31801982511830806 100-101 0.3374294858062799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2310705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.23117186871468 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98315614408267 30.821642106419954 2 11.751057768686088 9.925218805371543 3 5.115917434142003 6.481535652822143 4 2.8246096351539047 4.771462998568481 5 1.765548330969428 3.7280587503846143 6 1.164742466697288 2.951306357633032 7 0.8216087575369121 2.4288250453867724 8 0.59749057721813 2.0186181805149115 9 0.4271407889498145 1.6234790463250202 >10 2.304633116131945 17.266806421434328 >50 0.14940590646844193 4.319221721254489 >100 0.08638322416026994 6.981916864711786 >500 0.0059631742176721155 1.7387639126248313 >1k 0.0019167345699660347 1.8095016763532368 >5k 1.0648525388700192E-4 0.34491967437818294 >10k+ 3.1945576166100576E-4 2.7887227858167223 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28616 1.2384099225128262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19264 0.8336849576211589 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14133 0.611631515057093 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7670 0.33193332770734474 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4726 0.20452632421706796 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3591 0.1554071160100489 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3507 0.15177186183437522 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3492 0.15112270930300492 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.15068994094875807 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2972 0.128618754882168 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.3276835424686403E-5 0.0 10-11 0.0 0.0 0.0 4.3276835424686403E-5 0.0 12-13 0.0 4.3276835424686403E-5 0.0 6.49152531370296E-5 0.0 14-15 0.0 4.3276835424686403E-5 0.0 1.9474575941108883E-4 0.0 16-17 0.0 4.3276835424686403E-5 0.0 4.976836073838937E-4 0.0 18-19 0.0 4.3276835424686403E-5 0.0 7.357062022196689E-4 0.0 20-21 0.0 4.3276835424686403E-5 0.0 0.001060282467904817 0.0 22-23 0.0 4.3276835424686403E-5 0.0 0.0030510168974403917 0.0 24-25 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.006751186326251079 0.0 26-27 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.01425971727243417 0.0 28-29 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.03330152485929619 0.0 30-31 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.09512248426346072 0.0 32-33 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.20822649364587864 0.0 34-35 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.3665331576293815 0.0 36-37 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.6133193116386557 0.0 38-39 0.0 4.3276835424686403E-5 4.3276835424686403E-5 0.9936794181862245 0.0 40-41 0.0 4.3276835424686403E-5 4.3276835424686403E-5 1.445770879450211 0.0 42-43 0.0 4.3276835424686403E-5 4.3276835424686403E-5 1.8468173133307801 0.0 44-45 0.0 4.3276835424686403E-5 4.3276835424686403E-5 2.2930447633947217 0.0 46-47 0.0 4.3276835424686403E-5 4.3276835424686403E-5 2.8129077489337666 0.0 48-49 0.0 4.3276835424686403E-5 4.3276835424686403E-5 3.377280959707102 0.0 50-51 0.0 4.3276835424686403E-5 4.3276835424686403E-5 4.018016146587296 0.0 52-53 0.0 4.3276835424686403E-5 4.3276835424686403E-5 4.591477492799816 0.0 54-55 0.0 1.0819208856171601E-4 4.3276835424686403E-5 5.148320534209256 0.0 56-57 0.0 1.298305062740592E-4 4.3276835424686403E-5 5.779361709954321 0.0 58-59 0.0 1.298305062740592E-4 4.3276835424686403E-5 6.466987348017163 0.0 60-61 0.0 1.298305062740592E-4 4.3276835424686403E-5 7.230672024338892 0.0 62-63 0.0 1.298305062740592E-4 4.3276835424686403E-5 7.954606927323047 0.0 64-65 0.0 1.298305062740592E-4 4.3276835424686403E-5 8.620096464066162 0.0 66-67 0.0 1.298305062740592E-4 4.3276835424686403E-5 9.325270858893713 0.0 68-69 0.0 1.298305062740592E-4 4.3276835424686403E-5 10.104665026474605 0.0 70-71 0.0 1.514689239864024E-4 4.3276835424686403E-5 10.96500851471737 0.0 72-73 0.0 1.7310734169874561E-4 4.3276835424686403E-5 11.798953133351077 0.0 74-75 0.0 1.7310734169874561E-4 4.3276835424686403E-5 12.58124684890542 0.0 76-77 0.0 1.9474575941108883E-4 4.3276835424686403E-5 13.351574519464839 0.0 78-79 0.0 2.1638417712343202E-4 4.3276835424686403E-5 14.202267273407898 0.0 80-81 0.0 2.1638417712343202E-4 4.3276835424686403E-5 15.078233699238977 0.0 82-83 0.0 2.1638417712343202E-4 4.3276835424686403E-5 15.899195267245277 0.0 84-85 0.0 2.596610125481184E-4 4.3276835424686403E-5 16.661365254327144 0.0 86-87 0.0 2.596610125481184E-4 4.3276835424686403E-5 17.40276236040516 0.0 88-89 0.0 2.8129943026046164E-4 4.3276835424686403E-5 18.178932403746906 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 25975 0.0 36.693455 1 GGTATCA 15510 0.0 35.359837 1 TATCAAC 32820 0.0 29.979542 2 GTACATG 23110 0.0 29.705223 1 TACATGG 23655 0.0 29.682026 2 ATCAACG 33395 0.0 29.523373 3 TCAACGC 33695 0.0 29.330921 4 CAACGCA 33855 0.0 29.051525 5 AACGCAG 34425 0.0 28.5705 6 ACATGGG 23530 0.0 28.169321 3 CGTCACT 455 0.0 28.152477 1 ATGGGAG 5470 0.0 26.19462 5 CATGGGA 17250 0.0 26.157324 4 ATGGGCG 365 0.0 25.997381 5 ACGCAGA 38140 0.0 25.601013 7 CGCAGAG 38300 0.0 25.370184 8 AACGCGG 225 1.03682396E-10 25.30412 6 ATGGGAT 6245 0.0 24.767242 5 TAAGGTG 735 0.0 24.529501 5 CATGGGG 5510 0.0 24.455055 4 >>END_MODULE