FastQCFastQC Report
Fri 27 May 2016
SRR939861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310705
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303051.3115044975451216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230330.9967953503368019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179820.7782040546067109No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92450.4000943435012258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.24763005230005558No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.19591423396755533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41580.17994508169584608No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40820.1766560422035699No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.1573112967687351No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.14277028006604045No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.12615197526296087No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG26780.11589536526731019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153100.040.053951
GTATCAA254900.035.568111
CGTCACT4150.028.4164051
GTACATG237200.028.3385351
TACATGG237750.027.6729642
ATCAACG325300.027.647713
TATCAAC327450.027.5387922
TCAACGC328200.027.4028224
ACATGGG234700.027.1076243
CAACGCA333550.026.9350155
AACGCAG337350.026.6288726
ATGGGAG54900.025.5992415
CATGGGG55250.025.4370754
GTCGGGA13450.025.2471432
CATGGGA169950.025.1691934
CGGGAGT14100.023.413274
CGCAGAG381850.023.3399498
ACGCAGA384700.023.2282947
GAGTACT205100.022.65752212-13
AGTACTT207750.022.41388112-13
Produced by FastQC (version 0.11.5)